Standard name
Human Ortholog
Description Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.21 0.19 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0.09 0.16 0 0.07 0.14 0 0 0 0 0 0
Mitochondria 0.81 0.83 0.81 0.88 0.84 0.91 0.86 0.86 – 0.92 0.93 0.95 0.49 0.45 0.36 0.13 0.18 0.06 0.76 0.65 0.79 0.43 0.36 0.32
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0.05 0.06 0 0 0 0 0.07 0.1 0.13
Nuclear Periphery 0 0 0.07 0 0 0.05 0 0 – 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.13 0.12 0.12 0.08 0.16 0.16 0.19 – 0.16 0.12 0.15 0.16 0.14 0.1 0.21 0.24 0.25 0 0.05 0 0.18 0.22 0.16
Peroxisomes 0 0 0.08 0 0 0 0 0 – 0 0 0 0.06 0.06 0.08 0.26 0.15 0.17 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.09 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.1 0.09 0.06 0.11 0 0.09 0 – 0 0 0 0.28 0.33 0.38 0.05 0.21 0.19 0.06 0.14 0 0.11 0.13 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 – 0 0 0 1 7 4 0 0 0 3 1 5 3 2 2
Bud 2 3 2 1 4 7 7 10 – 5 7 11 3 5 3 1 1 0 4 5 2 8 12 17
Bud Neck 0 0 0 1 0 0 0 2 – 0 0 0 1 0 0 0 0 0 1 1 2 5 12 14
Bud Site 1 5 1 0 1 4 1 5 – 3 3 3 0 0 2 2 0 0 – – – – – –
Cell Periphery 1 3 2 6 2 0 1 2 – 2 0 2 4 10 10 0 0 0 1 0 0 0 0 0
Cytoplasm 0 2 4 2 9 3 7 4 – 2 6 2 7 10 14 8 13 4 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 1 1 1 – 4 3 2 1 0 1 0 2 0 0 1 0 1 1 2
Endosome 4 0 9 0 10 3 0 2 – 1 0 1 8 24 10 2 5 2 3 6 7 5 5 5
Golgi 1 0 5 5 5 0 2 2 – 2 2 2 27 59 69 1 5 5 10 9 10 6 6 7
Mitochondria 188 192 223 166 238 384 362 513 – 514 339 398 170 288 152 5 12 2 204 160 254 115 120 118
Nucleus 4 10 0 2 3 1 6 3 – 1 3 2 21 24 9 2 4 1 2 6 2 19 32 48
Nuclear Periphery 4 4 19 4 8 20 15 24 – 36 24 22 2 16 12 0 1 1 1 0 2 3 3 4
Nucleolus 40 29 32 23 24 66 69 114 – 92 42 62 55 87 43 8 16 9 10 13 8 47 72 59
Peroxisomes 5 5 23 4 3 5 1 6 – 6 2 2 22 36 32 10 10 6 5 1 12 10 13 12
SpindlePole 3 0 9 1 2 2 3 5 – 2 0 4 6 14 9 3 6 4 0 1 4 3 5 5
Vac/Vac Membrane 34 22 24 11 30 18 36 14 – 16 9 7 98 208 162 2 14 7 14 34 7 30 43 63
Unique Cell Count 232 231 276 188 284 422 419 597 560 363 418 345 634 424 39 68 36 268 248 321 266 335 370
Labelled Cell Count 287 275 354 227 339 514 511 707 686 440 520 426 788 532 44 89 41 268 248 321 266 335 370


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.6 10.7 11.6 13.7 13.0 12.5 14.3 13.4 – 13.8 15.0 14.3 16.3 15.9 15.3 10.3 14.8 14.9 15.3 15.5 15.8
Std Deviation (1e-4) 2.5 2.9 4.5 4.5 3.6 4.3 5.8 4.6 – 5.0 6.1 5.3 6.1 5.9 5.6 4.2 9.1 5.3 7.0 10.3 9.0
Intensity Change (Log2) – – – 0.24 0.17 0.11 0.3 0.21 – 0.26 0.37 0.31 0.5 0.46 0.41 -0.16 0.35 0.37 0.4 0.42 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 1.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 0 0 0 – 0 0 0 0 0 1.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0.2 0 0 0 – 0 0 0 -0.7 0.4 -0.7 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 3.4 4.1 6.1 0 0 0
Mitochondria 2.2 0.9 3.9 2.0 1.9 – 4.6 4.8 6.1 -8.1 -9.9 -11.7 -8.9 -10.0 -9.5
Nucleus 0 0 0 0 0 – 0 0 0 4.2 3.3 0 0 0 0
Nuclear Periphery -2.3 -2.2 -1.2 -2.0 -1.8 – -0.2 -0.1 -0.9 -4.3 -3.1 -2.5 0 0 0
Nucleolus 0.2 -1.2 1.5 1.8 2.8 – 1.8 0 1.2 1.6 0.9 -0.6 0 2.5 0
Peroxisomes -2.8 -4.1 -4.7 -5.7 -5.6 – -5.4 -5.0 -5.4 -0.9 -1.5 -0.4 0 1.6 0
SpindlePole 0 -2.2 0 0 0 – 0 0 -2.2 -1.2 -0.9 -0.9 0 0 0
Vacuole -1.1 0.7 -2.4 0 -4.3 – -3.7 -3.5 -4.4 6.1 7.7 8.6 0 2.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.4663 24.7957 24.4066 20.3696 23.436 23.8481 33.4861 31.2626 29.2654 33.0315 21.1472 30.0418 38.4733 31.7547 33.8673 33.2593 31.4694 37.7394
Actin 0.0036 0.0014 0.0091 0.0068 0.0004 0.0012 0.0118 0.0031 0.0007 0.0001 0.0072 0.0005 0.007 0.004 0.0123 0.0001 0.0034 0.0001
Bud 0.0003 0.0004 0.0006 0.0001 0.0001 0.0001 0.0001 0.0005 0.0004 0 0.0005 0 0.0001 0.0002 0.0003 0 0.0002 0
Bud Neck 0.0041 0.0001 0.0003 0.0003 0 0.0001 0.0005 0.0001 0.0001 0 0.0004 0 0.0001 0 0.0005 0 0.0002 0
Bud Periphery 0.0006 0.0005 0.0011 0.0001 0.0001 0.0002 0.0002 0.0008 0.0006 0.0002 0.0012 0.0001 0.0002 0.0003 0.0005 0 0.0005 0
Bud Site 0.0024 0.0005 0.008 0.0004 0.0002 0.0001 0.0005 0.0012 0.003 0 0.0012 0.0001 0.0004 0.0002 0.0008 0 0.0002 0
Cell Periphery 0.0013 0.0013 0.0008 0.0001 0.0001 0.0002 0.0003 0.0009 0.003 0.0003 0.0006 0.0005 0.0004 0.0003 0.0004 0.0001 0.0007 0.0002
Cytoplasm 0.002 0 0.0005 0.0032 0.0014 0.0029 0.0001 0 0.0001 0 0.0001 0.0154 0.0001 0 0.0005 0 0.0008 0
Cytoplasmic Foci 0.0351 0.0004 0.0006 0.0087 0.0261 0.0033 0.0006 0.0008 0.0012 0 0.0674 0.0015 0.0074 0.0001 0.0025 0.0002 0.0137 0.0001
Eisosomes 0.0004 0.0003 0.0007 0.0006 0.0004 0.0001 0.0005 0.0007 0.0003 0.0001 0.0006 0.0004 0.0005 0.0002 0.002 0.0002 0.0006 0.0001
Endoplasmic Reticulum 0.0067 0 0.0003 0.0023 0.0005 0.0038 0.0001 0 0.0001 0 0.0001 0.0014 0.0002 0 0.0002 0 0.0019 0
Endosome 0.01 0.0012 0.0022 0.0142 0.0024 0.0033 0.0056 0.0027 0.0016 0.0004 0.0086 0.0217 0.0019 0.0004 0.0015 0.0002 0.0092 0.0001
Golgi 0.0023 0.0011 0.0034 0.0122 0.0007 0.001 0.0015 0.0068 0.0011 0.0001 0.013 0.0005 0.0024 0.0006 0.0031 0.0005 0.003 0.0003
Lipid Particles 0.0145 0.0059 0.0039 0.0243 0.0091 0.0024 0.0038 0.009 0.0038 0.0008 0.0298 0.0014 0.01 0.0014 0.002 0.0052 0.0297 0.0065
Mitochondria 0.8293 0.9307 0.9202 0.8891 0.9369 0.9507 0.9601 0.9447 0.9593 0.9516 0.7796 0.9332 0.941 0.9773 0.9539 0.9902 0.8687 0.9616
None 0.0024 0 0.0003 0.0205 0.0062 0.0005 0.0001 0 0.0001 0 0.0001 0.0009 0.0005 0 0.0009 0 0.003 0
Nuclear Periphery 0.0018 0.0011 0.0011 0.0045 0.0012 0.0053 0.0001 0.0001 0.0001 0.0001 0.0003 0.0046 0.0007 0 0.0004 0 0.0283 0
Nucleolus 0.0165 0.0054 0.0012 0.0007 0.0017 0.0063 0.0004 0.0006 0.001 0.0002 0.0011 0.0007 0.0042 0.0002 0.0015 0 0.0032 0.0002
Nucleus 0.0018 0.0008 0.0005 0.0014 0.0008 0.0025 0.0001 0.0002 0.0003 0.0001 0.0004 0.003 0.0008 0.0001 0.0003 0 0.0035 0
Peroxisomes 0.0045 0.0019 0.0014 0.0047 0.0012 0.0004 0.0011 0.0029 0.002 0.0002 0.02 0.0006 0.0081 0.0005 0.0014 0.001 0.0168 0.0004
Punctate Nuclear 0.0151 0 0.0006 0.004 0.0089 0.0003 0 0 0 0 0.0003 0.0005 0.0013 0 0.0005 0 0.0015 0
Vacuole 0.0098 0.0121 0.0085 0.0005 0.0006 0.0027 0.0035 0.0052 0.0078 0.0097 0.0126 0.0059 0.0026 0.0022 0.0021 0.0003 0.003 0.0021
Vacuole Periphery 0.0356 0.0348 0.0346 0.0014 0.001 0.0126 0.0092 0.0196 0.0136 0.036 0.0548 0.0072 0.01 0.0119 0.0125 0.002 0.008 0.0282

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 110.6975 98.9568 91.9852 95.7091 98.3835 120.7482 130.9862 132.1564 130.1989 138.0815
Translational Efficiency 1.702 1.6889 1.4608 1.3615 1.169 1.5092 1.241 1.2175 1.4229 1.3472

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘