Standard name | |
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Human Ortholog | |
Description | Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 7 | 4 | 0 | 0 | 0 | 3 | 1 | 5 | 3 | 2 | 2 |
Bud | 2 | 3 | 2 | 1 | 4 | 7 | 7 | 10 | – | 5 | 7 | 11 | 3 | 5 | 3 | 1 | 1 | 0 | 4 | 5 | 2 | 8 | 12 | 17 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 5 | 12 | 14 |
Bud Site | 1 | 5 | 1 | 0 | 1 | 4 | 1 | 5 | – | 3 | 3 | 3 | 0 | 0 | 2 | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 2 | 6 | 2 | 0 | 1 | 2 | – | 2 | 0 | 2 | 4 | 10 | 10 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2 | 4 | 2 | 9 | 3 | 7 | 4 | – | 2 | 6 | 2 | 7 | 10 | 14 | 8 | 13 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 4 | 3 | 2 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 2 |
Endosome | 4 | 0 | 9 | 0 | 10 | 3 | 0 | 2 | – | 1 | 0 | 1 | 8 | 24 | 10 | 2 | 5 | 2 | 3 | 6 | 7 | 5 | 5 | 5 |
Golgi | 1 | 0 | 5 | 5 | 5 | 0 | 2 | 2 | – | 2 | 2 | 2 | 27 | 59 | 69 | 1 | 5 | 5 | 10 | 9 | 10 | 6 | 6 | 7 |
Mitochondria | 188 | 192 | 223 | 166 | 238 | 384 | 362 | 513 | – | 514 | 339 | 398 | 170 | 288 | 152 | 5 | 12 | 2 | 204 | 160 | 254 | 115 | 120 | 118 |
Nucleus | 4 | 10 | 0 | 2 | 3 | 1 | 6 | 3 | – | 1 | 3 | 2 | 21 | 24 | 9 | 2 | 4 | 1 | 2 | 6 | 2 | 19 | 32 | 48 |
Nuclear Periphery | 4 | 4 | 19 | 4 | 8 | 20 | 15 | 24 | – | 36 | 24 | 22 | 2 | 16 | 12 | 0 | 1 | 1 | 1 | 0 | 2 | 3 | 3 | 4 |
Nucleolus | 40 | 29 | 32 | 23 | 24 | 66 | 69 | 114 | – | 92 | 42 | 62 | 55 | 87 | 43 | 8 | 16 | 9 | 10 | 13 | 8 | 47 | 72 | 59 |
Peroxisomes | 5 | 5 | 23 | 4 | 3 | 5 | 1 | 6 | – | 6 | 2 | 2 | 22 | 36 | 32 | 10 | 10 | 6 | 5 | 1 | 12 | 10 | 13 | 12 |
SpindlePole | 3 | 0 | 9 | 1 | 2 | 2 | 3 | 5 | – | 2 | 0 | 4 | 6 | 14 | 9 | 3 | 6 | 4 | 0 | 1 | 4 | 3 | 5 | 5 |
Vac/Vac Membrane | 34 | 22 | 24 | 11 | 30 | 18 | 36 | 14 | – | 16 | 9 | 7 | 98 | 208 | 162 | 2 | 14 | 7 | 14 | 34 | 7 | 30 | 43 | 63 |
Unique Cell Count | 232 | 231 | 276 | 188 | 284 | 422 | 419 | 597 | 560 | 363 | 418 | 345 | 634 | 424 | 39 | 68 | 36 | 268 | 248 | 321 | 266 | 335 | 370 | |
Labelled Cell Count | 287 | 275 | 354 | 227 | 339 | 514 | 511 | 707 | 686 | 440 | 520 | 426 | 788 | 532 | 44 | 89 | 41 | 268 | 248 | 321 | 266 | 335 | 370 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.6 | 10.7 | 11.6 | 13.7 | 13.0 | 12.5 | 14.3 | 13.4 | – | 13.8 | 15.0 | 14.3 | 16.3 | 15.9 | 15.3 | 10.3 | 14.8 | 14.9 | 15.3 | 15.5 | 15.8 |
Std Deviation (1e-4) | 2.5 | 2.9 | 4.5 | 4.5 | 3.6 | 4.3 | 5.8 | 4.6 | – | 5.0 | 6.1 | 5.3 | 6.1 | 5.9 | 5.6 | 4.2 | 9.1 | 5.3 | 7.0 | 10.3 | 9.0 |
Intensity Change (Log2) | – | – | – | 0.24 | 0.17 | 0.11 | 0.3 | 0.21 | – | 0.26 | 0.37 | 0.31 | 0.5 | 0.46 | 0.41 | -0.16 | 0.35 | 0.37 | 0.4 | 0.42 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | -0.7 | 0.4 | -0.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.4 | 4.1 | 6.1 | 0 | 0 | 0 |
Mitochondria | 2.2 | 0.9 | 3.9 | 2.0 | 1.9 | – | 4.6 | 4.8 | 6.1 | -8.1 | -9.9 | -11.7 | -8.9 | -10.0 | -9.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.2 | 3.3 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.3 | -2.2 | -1.2 | -2.0 | -1.8 | – | -0.2 | -0.1 | -0.9 | -4.3 | -3.1 | -2.5 | 0 | 0 | 0 |
Nucleolus | 0.2 | -1.2 | 1.5 | 1.8 | 2.8 | – | 1.8 | 0 | 1.2 | 1.6 | 0.9 | -0.6 | 0 | 2.5 | 0 |
Peroxisomes | -2.8 | -4.1 | -4.7 | -5.7 | -5.6 | – | -5.4 | -5.0 | -5.4 | -0.9 | -1.5 | -0.4 | 0 | 1.6 | 0 |
SpindlePole | 0 | -2.2 | 0 | 0 | 0 | – | 0 | 0 | -2.2 | -1.2 | -0.9 | -0.9 | 0 | 0 | 0 |
Vacuole | -1.1 | 0.7 | -2.4 | 0 | -4.3 | – | -3.7 | -3.5 | -4.4 | 6.1 | 7.7 | 8.6 | 0 | 2.8 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.4663 | 24.7957 | 24.4066 | 20.3696 | 23.436 | 23.8481 | 33.4861 | 31.2626 | 29.2654 | 33.0315 | 21.1472 | 30.0418 | 38.4733 | 31.7547 | 33.8673 | 33.2593 | 31.4694 | 37.7394 |
Actin | 0.0036 | 0.0014 | 0.0091 | 0.0068 | 0.0004 | 0.0012 | 0.0118 | 0.0031 | 0.0007 | 0.0001 | 0.0072 | 0.0005 | 0.007 | 0.004 | 0.0123 | 0.0001 | 0.0034 | 0.0001 |
Bud | 0.0003 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0004 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 |
Bud Neck | 0.0041 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0 |
Bud Periphery | 0.0006 | 0.0005 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0008 | 0.0006 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0 | 0.0005 | 0 |
Bud Site | 0.0024 | 0.0005 | 0.008 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0012 | 0.003 | 0 | 0.0012 | 0.0001 | 0.0004 | 0.0002 | 0.0008 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0013 | 0.0013 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0009 | 0.003 | 0.0003 | 0.0006 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0002 |
Cytoplasm | 0.002 | 0 | 0.0005 | 0.0032 | 0.0014 | 0.0029 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0154 | 0.0001 | 0 | 0.0005 | 0 | 0.0008 | 0 |
Cytoplasmic Foci | 0.0351 | 0.0004 | 0.0006 | 0.0087 | 0.0261 | 0.0033 | 0.0006 | 0.0008 | 0.0012 | 0 | 0.0674 | 0.0015 | 0.0074 | 0.0001 | 0.0025 | 0.0002 | 0.0137 | 0.0001 |
Eisosomes | 0.0004 | 0.0003 | 0.0007 | 0.0006 | 0.0004 | 0.0001 | 0.0005 | 0.0007 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.002 | 0.0002 | 0.0006 | 0.0001 |
Endoplasmic Reticulum | 0.0067 | 0 | 0.0003 | 0.0023 | 0.0005 | 0.0038 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0014 | 0.0002 | 0 | 0.0002 | 0 | 0.0019 | 0 |
Endosome | 0.01 | 0.0012 | 0.0022 | 0.0142 | 0.0024 | 0.0033 | 0.0056 | 0.0027 | 0.0016 | 0.0004 | 0.0086 | 0.0217 | 0.0019 | 0.0004 | 0.0015 | 0.0002 | 0.0092 | 0.0001 |
Golgi | 0.0023 | 0.0011 | 0.0034 | 0.0122 | 0.0007 | 0.001 | 0.0015 | 0.0068 | 0.0011 | 0.0001 | 0.013 | 0.0005 | 0.0024 | 0.0006 | 0.0031 | 0.0005 | 0.003 | 0.0003 |
Lipid Particles | 0.0145 | 0.0059 | 0.0039 | 0.0243 | 0.0091 | 0.0024 | 0.0038 | 0.009 | 0.0038 | 0.0008 | 0.0298 | 0.0014 | 0.01 | 0.0014 | 0.002 | 0.0052 | 0.0297 | 0.0065 |
Mitochondria | 0.8293 | 0.9307 | 0.9202 | 0.8891 | 0.9369 | 0.9507 | 0.9601 | 0.9447 | 0.9593 | 0.9516 | 0.7796 | 0.9332 | 0.941 | 0.9773 | 0.9539 | 0.9902 | 0.8687 | 0.9616 |
None | 0.0024 | 0 | 0.0003 | 0.0205 | 0.0062 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0009 | 0.0005 | 0 | 0.0009 | 0 | 0.003 | 0 |
Nuclear Periphery | 0.0018 | 0.0011 | 0.0011 | 0.0045 | 0.0012 | 0.0053 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0046 | 0.0007 | 0 | 0.0004 | 0 | 0.0283 | 0 |
Nucleolus | 0.0165 | 0.0054 | 0.0012 | 0.0007 | 0.0017 | 0.0063 | 0.0004 | 0.0006 | 0.001 | 0.0002 | 0.0011 | 0.0007 | 0.0042 | 0.0002 | 0.0015 | 0 | 0.0032 | 0.0002 |
Nucleus | 0.0018 | 0.0008 | 0.0005 | 0.0014 | 0.0008 | 0.0025 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.003 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0035 | 0 |
Peroxisomes | 0.0045 | 0.0019 | 0.0014 | 0.0047 | 0.0012 | 0.0004 | 0.0011 | 0.0029 | 0.002 | 0.0002 | 0.02 | 0.0006 | 0.0081 | 0.0005 | 0.0014 | 0.001 | 0.0168 | 0.0004 |
Punctate Nuclear | 0.0151 | 0 | 0.0006 | 0.004 | 0.0089 | 0.0003 | 0 | 0 | 0 | 0 | 0.0003 | 0.0005 | 0.0013 | 0 | 0.0005 | 0 | 0.0015 | 0 |
Vacuole | 0.0098 | 0.0121 | 0.0085 | 0.0005 | 0.0006 | 0.0027 | 0.0035 | 0.0052 | 0.0078 | 0.0097 | 0.0126 | 0.0059 | 0.0026 | 0.0022 | 0.0021 | 0.0003 | 0.003 | 0.0021 |
Vacuole Periphery | 0.0356 | 0.0348 | 0.0346 | 0.0014 | 0.001 | 0.0126 | 0.0092 | 0.0196 | 0.0136 | 0.036 | 0.0548 | 0.0072 | 0.01 | 0.0119 | 0.0125 | 0.002 | 0.008 | 0.0282 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 110.6975 | 98.9568 | 91.9852 | 95.7091 | 98.3835 | 120.7482 | 130.9862 | 132.1564 | 130.1989 | 138.0815 |
Translational Efficiency | 1.702 | 1.6889 | 1.4608 | 1.3615 | 1.169 | 1.5092 | 1.241 | 1.2175 | 1.4229 | 1.3472 |
Dataset | Hit |
---|---|
Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |