Standard name
Description Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.21 0.19 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0.09 0.16 0 0.07 0.14 0 0 0 0 0 0
Mitochondria 0.81 0.83 0.81 0.88 0.84 0.91 0.86 0.86 – 0.92 0.93 0.95 0.49 0.45 0.36 0.13 0.18 0.06 0.76 0.65 0.79 0.43 0.36 0.32
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0.05 0.06 0 0 0 0 0.07 0.1 0.13
Nuclear Periphery 0 0 0.07 0 0 0.05 0 0 – 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.13 0.12 0.12 0.08 0.16 0.16 0.19 – 0.16 0.12 0.15 0.16 0.14 0.1 0.21 0.24 0.25 0 0.05 0 0.18 0.22 0.16
Peroxisomes 0 0 0.08 0 0 0 0 0 – 0 0 0 0.06 0.06 0.08 0.26 0.15 0.17 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.09 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.1 0.09 0.06 0.11 0 0.09 0 – 0 0 0 0.28 0.33 0.38 0.05 0.21 0.19 0.06 0.14 0 0.11 0.13 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 – 0 0 0 1 7 4 0 0 0 3 1 5 3 2 2
Bud 2 3 2 1 4 7 7 10 – 5 7 11 3 5 3 1 1 0 4 5 2 8 12 17
Bud Neck 0 0 0 1 0 0 0 2 – 0 0 0 1 0 0 0 0 0 1 1 2 5 12 14
Bud Site 1 5 1 0 1 4 1 5 – 3 3 3 0 0 2 2 0 0 – – – – – –
Cell Periphery 1 3 2 6 2 0 1 2 – 2 0 2 4 10 10 0 0 0 1 0 0 0 0 0
Cytoplasm 0 2 4 2 9 3 7 4 – 2 6 2 7 10 14 8 13 4 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 1 1 1 – 4 3 2 1 0 1 0 2 0 0 1 0 1 1 2
Endosome 4 0 9 0 10 3 0 2 – 1 0 1 8 24 10 2 5 2 3 6 7 5 5 5
Golgi 1 0 5 5 5 0 2 2 – 2 2 2 27 59 69 1 5 5 10 9 10 6 6 7
Mitochondria 188 192 223 166 238 384 362 513 – 514 339 398 170 288 152 5 12 2 204 160 254 115 120 118
Nucleus 4 10 0 2 3 1 6 3 – 1 3 2 21 24 9 2 4 1 2 6 2 19 32 48
Nuclear Periphery 4 4 19 4 8 20 15 24 – 36 24 22 2 16 12 0 1 1 1 0 2 3 3 4
Nucleolus 40 29 32 23 24 66 69 114 – 92 42 62 55 87 43 8 16 9 10 13 8 47 72 59
Peroxisomes 5 5 23 4 3 5 1 6 – 6 2 2 22 36 32 10 10 6 5 1 12 10 13 12
SpindlePole 3 0 9 1 2 2 3 5 – 2 0 4 6 14 9 3 6 4 0 1 4 3 5 5
Vac/Vac Membrane 34 22 24 11 30 18 36 14 – 16 9 7 98 208 162 2 14 7 14 34 7 30 43 63
Unique Cell Count 232 231 276 188 284 422 419 597 560 363 418 345 634 424 39 68 36 268 248 321 266 335 370
Labelled Cell Count 287 275 354 227 339 514 511 707 686 440 520 426 788 532 44 89 41 268 248 321 266 335 370


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.6 10.7 11.6 13.7 13.0 12.5 14.3 13.4 – 13.8 15.0 14.3 16.3 15.9 15.3 10.3 14.8 14.9 15.3 15.5 15.8
Std Deviation (1e-4) 2.5 2.9 4.5 4.5 3.6 4.3 5.8 4.6 – 5.0 6.1 5.3 6.1 5.9 5.6 4.2 9.1 5.3 7.0 10.3 9.0
Intensity Change (Log2) – – – 0.24 0.17 0.11 0.3 0.21 – 0.26 0.37 0.31 0.5 0.46 0.41 -0.16 0.35 0.37 0.4 0.42 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 1.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 0 0 0 – 0 0 0 0 0 1.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0.2 0 0 0 – 0 0 0 -0.7 0.4 -0.7 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 3.4 4.1 6.1 0 0 0
Mitochondria 2.2 0.9 3.9 2.0 1.9 – 4.6 4.8 6.1 -8.1 -9.9 -11.7 -8.9 -10.0 -9.5
Nucleus 0 0 0 0 0 – 0 0 0 4.2 3.3 0 0 0 0
Nuclear Periphery -2.3 -2.2 -1.2 -2.0 -1.8 – -0.2 -0.1 -0.9 -4.3 -3.1 -2.5 0 0 0
Nucleolus 0.2 -1.2 1.5 1.8 2.8 – 1.8 0 1.2 1.6 0.9 -0.6 0 2.5 0
Peroxisomes -2.8 -4.1 -4.7 -5.7 -5.6 – -5.4 -5.0 -5.4 -0.9 -1.5 -0.4 0 1.6 0
SpindlePole 0 -2.2 0 0 0 – 0 0 -2.2 -1.2 -0.9 -0.9 0 0 0
Vacuole -1.1 0.7 -2.4 0 -4.3 – -3.7 -3.5 -4.4 6.1 7.7 8.6 0 2.8 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tom70-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available