Standard name | |
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Description | Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 7 | 4 | 0 | 0 | 0 | 3 | 1 | 5 | 3 | 2 | 2 |
Bud | 2 | 3 | 2 | 1 | 4 | 7 | 7 | 10 | – | 5 | 7 | 11 | 3 | 5 | 3 | 1 | 1 | 0 | 4 | 5 | 2 | 8 | 12 | 17 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 5 | 12 | 14 |
Bud Site | 1 | 5 | 1 | 0 | 1 | 4 | 1 | 5 | – | 3 | 3 | 3 | 0 | 0 | 2 | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 2 | 6 | 2 | 0 | 1 | 2 | – | 2 | 0 | 2 | 4 | 10 | 10 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2 | 4 | 2 | 9 | 3 | 7 | 4 | – | 2 | 6 | 2 | 7 | 10 | 14 | 8 | 13 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 4 | 3 | 2 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 2 |
Endosome | 4 | 0 | 9 | 0 | 10 | 3 | 0 | 2 | – | 1 | 0 | 1 | 8 | 24 | 10 | 2 | 5 | 2 | 3 | 6 | 7 | 5 | 5 | 5 |
Golgi | 1 | 0 | 5 | 5 | 5 | 0 | 2 | 2 | – | 2 | 2 | 2 | 27 | 59 | 69 | 1 | 5 | 5 | 10 | 9 | 10 | 6 | 6 | 7 |
Mitochondria | 188 | 192 | 223 | 166 | 238 | 384 | 362 | 513 | – | 514 | 339 | 398 | 170 | 288 | 152 | 5 | 12 | 2 | 204 | 160 | 254 | 115 | 120 | 118 |
Nucleus | 4 | 10 | 0 | 2 | 3 | 1 | 6 | 3 | – | 1 | 3 | 2 | 21 | 24 | 9 | 2 | 4 | 1 | 2 | 6 | 2 | 19 | 32 | 48 |
Nuclear Periphery | 4 | 4 | 19 | 4 | 8 | 20 | 15 | 24 | – | 36 | 24 | 22 | 2 | 16 | 12 | 0 | 1 | 1 | 1 | 0 | 2 | 3 | 3 | 4 |
Nucleolus | 40 | 29 | 32 | 23 | 24 | 66 | 69 | 114 | – | 92 | 42 | 62 | 55 | 87 | 43 | 8 | 16 | 9 | 10 | 13 | 8 | 47 | 72 | 59 |
Peroxisomes | 5 | 5 | 23 | 4 | 3 | 5 | 1 | 6 | – | 6 | 2 | 2 | 22 | 36 | 32 | 10 | 10 | 6 | 5 | 1 | 12 | 10 | 13 | 12 |
SpindlePole | 3 | 0 | 9 | 1 | 2 | 2 | 3 | 5 | – | 2 | 0 | 4 | 6 | 14 | 9 | 3 | 6 | 4 | 0 | 1 | 4 | 3 | 5 | 5 |
Vac/Vac Membrane | 34 | 22 | 24 | 11 | 30 | 18 | 36 | 14 | – | 16 | 9 | 7 | 98 | 208 | 162 | 2 | 14 | 7 | 14 | 34 | 7 | 30 | 43 | 63 |
Unique Cell Count | 232 | 231 | 276 | 188 | 284 | 422 | 419 | 597 | 560 | 363 | 418 | 345 | 634 | 424 | 39 | 68 | 36 | 268 | 248 | 321 | 266 | 335 | 370 | |
Labelled Cell Count | 287 | 275 | 354 | 227 | 339 | 514 | 511 | 707 | 686 | 440 | 520 | 426 | 788 | 532 | 44 | 89 | 41 | 268 | 248 | 321 | 266 | 335 | 370 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 11.6 | 10.7 | 11.6 | 13.7 | 13.0 | 12.5 | 14.3 | 13.4 | – | 13.8 | 15.0 | 14.3 | 16.3 | 15.9 | 15.3 | 10.3 | 14.8 | 14.9 | 15.3 | 15.5 | 15.8 |
Std Deviation (1e-4) | 2.5 | 2.9 | 4.5 | 4.5 | 3.6 | 4.3 | 5.8 | 4.6 | – | 5.0 | 6.1 | 5.3 | 6.1 | 5.9 | 5.6 | 4.2 | 9.1 | 5.3 | 7.0 | 10.3 | 9.0 |
Intensity Change (Log2) | – | – | – | 0.24 | 0.17 | 0.11 | 0.3 | 0.21 | – | 0.26 | 0.37 | 0.31 | 0.5 | 0.46 | 0.41 | -0.16 | 0.35 | 0.37 | 0.4 | 0.42 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | -0.7 | 0.4 | -0.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.4 | 4.1 | 6.1 | 0 | 0 | 0 |
Mitochondria | 2.2 | 0.9 | 3.9 | 2.0 | 1.9 | – | 4.6 | 4.8 | 6.1 | -8.1 | -9.9 | -11.7 | -8.9 | -10.0 | -9.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.2 | 3.3 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.3 | -2.2 | -1.2 | -2.0 | -1.8 | – | -0.2 | -0.1 | -0.9 | -4.3 | -3.1 | -2.5 | 0 | 0 | 0 |
Nucleolus | 0.2 | -1.2 | 1.5 | 1.8 | 2.8 | – | 1.8 | 0 | 1.2 | 1.6 | 0.9 | -0.6 | 0 | 2.5 | 0 |
Peroxisomes | -2.8 | -4.1 | -4.7 | -5.7 | -5.6 | – | -5.4 | -5.0 | -5.4 | -0.9 | -1.5 | -0.4 | 0 | 1.6 | 0 |
SpindlePole | 0 | -2.2 | 0 | 0 | 0 | – | 0 | 0 | -2.2 | -1.2 | -0.9 | -0.9 | 0 | 0 | 0 |
Vacuole | -1.1 | 0.7 | -2.4 | 0 | -4.3 | – | -3.7 | -3.5 | -4.4 | 6.1 | 7.7 | 8.6 | 0 | 2.8 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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