Standard name
Human Ortholog
Description Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic12p whose assembly and stability requires cardiolipin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.08 0 0.08 0.09 0.14
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.46 0.34 0.7 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.1 0 0.11 0 0 0 0 0.05 0.05 0.08 0 0 0.05 0 0.05 0 0 0.06 0
Golgi 0 0 0.2 0.13 0.15 0 0 0 0 0 0 0.06 0.09 0.15 0.26 0.32 0.1 0.1 0.14 0.09 0.12 0.12 0.13
Mitochondria 0.91 0.94 0.82 0.89 0.73 0.88 0.66 0.9 0.86 0.88 0.87 0.85 0.84 0.77 0.1 0.18 0.1 0.7 0.59 0.61 0.36 0.39 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.1 0.05 0 0 0 0.06 0.09 0.06 0.06 0.06 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0.08 0.05 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0.07 0 0 0 0 0 0 0.19 0.15 0.17 0.05 0.08 0.05 0.1 0.09 0.2 0.33 0.29 0.28
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.05 0 0 0.08 0.06 0.15 0 0.07 0 0.05 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 4 6 0 1 2 1 0 0 1 4 5 2 2 2 0 3 30 7 18 26 42
Bud 2 3 4 2 5 11 5 11 8 11 8 3 4 8 0 0 0 0 1 1 1 1 0
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 2 0 1 0 1 1 4 3 3 0 1 1 0 0 0 0
Cell Periphery 1 16 8 3 6 1 4 4 1 5 4 5 3 0 3 0 0 0 2 0 0 0 0
Cytoplasm 1 3 1 7 8 9 20 8 11 9 11 6 9 8 18 13 14 0 0 0 0 1 1
Endoplasmic Reticulum 1 8 4 2 1 2 2 2 1 1 4 0 8 1 0 2 0 0 1 0 0 0 1
Endosome 8 2 11 4 21 3 43 5 5 13 2 16 19 40 1 1 1 8 17 8 9 15 9
Golgi 7 9 35 24 31 1 16 1 4 6 4 17 35 71 10 12 2 22 52 17 27 34 39
Mitochondria 187 328 141 162 155 287 263 272 254 419 355 259 309 376 4 7 2 157 223 112 79 109 113
Nucleus 0 1 0 0 2 5 3 2 3 8 4 0 0 2 5 1 1 0 0 0 0 0 0
Nuclear Periphery 21 16 6 5 8 18 38 19 17 29 34 11 15 12 0 0 1 0 1 0 4 2 2
Nucleolus 18 8 4 1 3 27 22 16 20 30 15 10 6 8 0 0 0 1 1 0 0 2 1
Peroxisomes 9 15 12 8 15 3 6 8 5 4 2 59 54 85 2 3 1 22 35 37 73 81 86
SpindlePole 1 0 4 3 6 7 16 9 5 8 7 6 2 4 1 0 0 0 1 0 1 0 2
Vac/Vac Membrane 22 19 4 8 18 18 61 13 20 17 21 13 14 23 2 3 0 3 3 1 1 2 1
Unique Cell Count 206 348 171 183 212 325 401 302 297 474 406 305 369 486 39 38 20 224 377 186 223 282 307
Labelled Cell Count 278 436 238 236 279 394 503 375 357 563 472 410 484 640 48 44 22 224 377 186 223 282 307


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 9.2 8.7 8.5 9.1 5.9 7.6 6.8 7.2 7.3 7.1 8.8 9.7 9.8 10.2 11.7 9.8 8.5 9.1 9.2
Std Deviation (1e-4) 1.6 1.7 1.6 1.8 2.1 1.5 1.7 1.8 1.7 1.9 1.7 1.6 1.8 1.9 2.9 3.1 3.3 2.4 2.3 2.5
Intensity Change (Log2) -0.02 0.06 -0.56 -0.2 -0.35 -0.28 -0.26 -0.29 0.02 0.16 0.17 0.23 0.42 0.17 -0.04 0.06 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.6 0 0.8 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.0 1.2 0 1.6 -2.8 -2.7 -2.2 0 -0.5 -0.6 0.8 0 0 0
Golgi -1.9 -1.5 -8.2 -6.3 -7.9 -7.2 -8.8 -8.5 -5.0 -3.5 -1.8 0.7 1.5 0
Mitochondria 1.6 -2.2 1.8 -4.1 2.4 0.9 2.0 1.6 0.7 0.4 -1.4 -8.8 -7.9 -7.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.4 0.1 1.0 2.5 1.3 1.1 1.3 2.1 0.1 0.3 0 0 0 0
Nucleolus 0 0 2.6 1.7 1.5 2.1 2.0 0.8 0.6 0 0 0 0 0
Peroxisomes -1.1 0 -3.8 -3.5 -2.3 -3.0 0 0 3.6 2.5 3.3 0 0 0
SpindlePole 0 0 0 1.0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 2.6 1.6 4.4 1.1 2.1 0.8 1.5 1.1 0.9 1.4 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0019 6.2464 5.1814 5.1368 4.8094 5.336 7.2533 8.6723 7.832 7.4543 7.3933 7.904 10.8011 11.1997 10.3815 10.4444 9.6752 9.9992
Actin 0.0451 0.019 0.0173 0.0073 0.0081 0.0156 0.0534 0.029 0.0142 0.0032 0.0049 0.0118 0.0196 0.0061 0.0183 0.0174 0.0031 0.008
Bud 0.0004 0.0006 0.0002 0.0001 0.0004 0.0001 0.0008 0.0013 0.0003 0.0001 0.0012 0.0001 0.0003 0.0005 0.0001 0.0001 0 0.0001
Bud Neck 0.001 0.0003 0.001 0.0001 0.0003 0.0015 0.001 0.0004 0.0002 0.0001 0.0003 0.0001 0.0002 0.0002 0.0003 0.0001 0 0.0001
Bud Periphery 0.0007 0.0009 0.0003 0.0001 0.0006 0.0003 0.0014 0.002 0.0005 0.0002 0.002 0.0002 0.0005 0.0005 0.0002 0.0001 0.0001 0.0004
Bud Site 0.0026 0.0013 0.0011 0.0002 0.0007 0.0002 0.0023 0.0086 0.0005 0.0001 0.0009 0.0001 0.0008 0.0029 0.0009 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0006 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0014 0.0001 0.0004 0.0003 0.0007 0.0016 0.0006 0.0002 0.0002 0 0.0004 0 0.0019 0.0004 0.0006 0 0 0.0001
Cytoplasmic Foci 0.0092 0.0041 0.0077 0.0095 0.0133 0.0042 0.0074 0.0062 0.0044 0.0036 0.0059 0.0019 0.0053 0.0046 0.0043 0.0019 0.0013 0.0022
Eisosomes 0.0009 0.0003 0.0004 0.0003 0.0003 0.0003 0.001 0.0006 0.001 0.0002 0.0014 0.0004 0.0007 0.0002 0.0008 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.002 0.0001 0.0005 0.0004 0.0048 0.0004 0.0011 0.0003 0.0002 0.0001 0.0004 0.0001 0.001 0.0001 0.0002 0 0.0001 0.0001
Endosome 0.0215 0.0133 0.0181 0.0118 0.0324 0.0088 0.0332 0.0183 0.0127 0.0065 0.0426 0.0129 0.0153 0.0215 0.0084 0.0026 0.0044 0.007
Golgi 0.0343 0.0868 0.0288 0.0109 0.0268 0.0391 0.0941 0.0817 0.0469 0.0202 0.0768 0.0626 0.0567 0.0551 0.0452 0.0117 0.0486 0.0555
Lipid Particles 0.0274 0.0096 0.0313 0.0113 0.0066 0.0107 0.0291 0.0149 0.0078 0.016 0.0028 0.0124 0.0398 0.0044 0.005 0.0016 0.0061 0.006
Mitochondria 0.7722 0.842 0.8712 0.9334 0.877 0.8943 0.713 0.803 0.8892 0.9387 0.8446 0.8888 0.8149 0.8797 0.8848 0.9412 0.9132 0.8934
None 0.0013 0.0001 0.0005 0.0004 0.0004 0.0019 0.0007 0.0001 0.0003 0 0.0001 0 0.0031 0 0.0004 0 0 0.0001
Nuclear Periphery 0.0174 0.0001 0.0012 0.0007 0.0074 0.0018 0.0041 0.0002 0.0017 0 0.0002 0.0001 0.0061 0.0001 0.0009 0 0.0003 0.0004
Nucleolus 0.0019 0.0002 0.0004 0.0001 0.0004 0.0002 0.0017 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0004 0.0002 0 0.0001 0.0001
Nucleus 0.0042 0.0001 0.0003 0.0001 0.0008 0.0015 0.0152 0.0002 0.0004 0 0.0002 0 0.0008 0.0001 0.0004 0 0.0001 0.0002
Peroxisomes 0.0418 0.0172 0.0128 0.0096 0.0109 0.0086 0.0167 0.0239 0.0159 0.0075 0.0092 0.0052 0.0251 0.0105 0.0234 0.0209 0.0164 0.0166
Punctate Nuclear 0.0034 0 0.0023 0.0013 0.0004 0.0056 0.0011 0.0001 0.0007 0 0.0001 0 0.0021 0 0.0038 0 0 0.0039
Vacuole 0.0032 0.0016 0.0013 0.0007 0.0027 0.0008 0.0117 0.0027 0.0007 0.0013 0.0026 0.0007 0.001 0.0058 0.0004 0.0004 0.0011 0.0019
Vacuole Periphery 0.0077 0.0022 0.0027 0.0016 0.0049 0.0024 0.0098 0.0053 0.0019 0.0019 0.003 0.0025 0.0044 0.0066 0.0012 0.0015 0.0044 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.3907 9.7938 9.3438 23.693 15.2193 12.514 21.2197 26.7341 23.6736 25.9613
Translational Efficiency 2.9775 2.6711 3.1724 1.3047 1.514 2.9952 1.6682 1.5836 1.6699 1.3525

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic12p whose assembly and stability requires cardiolipin
Localization
Cell Percentages mitochondrion (77%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mic27

Mic27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mic27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available