Standard name
Description Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic12p whose assembly and stability requires cardiolipin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0 0.08 0 0.08 0.09 0.14
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.46 0.34 0.7 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.1 0 0.11 – 0 0 0 0 0.05 0.05 0.08 0 0 0.05 0 0.05 0 0 0.06 0
Golgi 0 0 0.2 0.13 0.15 0 0 – 0 0 0 0 0.06 0.09 0.15 0.26 0.32 0.1 0.1 0.14 0.09 0.12 0.12 0.13
Mitochondria 0.91 0.94 0.82 0.89 0.73 0.88 0.66 – 0.9 0.86 0.88 0.87 0.85 0.84 0.77 0.1 0.18 0.1 0.7 0.59 0.61 0.36 0.39 0.37
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.13 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.1 0.05 0 0 0 0.06 0.09 – 0.06 0.06 0.06 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0.08 0.05 – 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0.07 0 0 – 0 0 0 0 0.19 0.15 0.17 0.05 0.08 0.05 0.1 0.09 0.2 0.33 0.29 0.28
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.05 0 0 0.08 0.06 0.15 – 0 0.07 0 0.05 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 4 6 0 1 2 – 1 0 0 1 4 5 2 2 2 0 3 30 7 18 26 42
Bud 2 3 4 2 5 11 5 – 11 8 11 8 3 4 8 0 0 0 0 1 1 1 1 0
Bud Neck 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 2 0 1 0 1 1 – 4 3 3 0 1 1 0 0 0 0 – – – – – –
Cell Periphery 1 16 8 3 6 1 4 – 4 1 5 4 5 3 0 3 0 0 0 2 0 0 0 0
Cytoplasm 1 3 1 7 8 9 20 – 8 11 9 11 6 9 8 18 13 14 0 0 0 0 1 1
Endoplasmic Reticulum 1 8 4 2 1 2 2 – 2 1 1 4 0 8 1 0 2 0 0 1 0 0 0 1
Endosome 8 2 11 4 21 3 43 – 5 5 13 2 16 19 40 1 1 1 8 17 8 9 15 9
Golgi 7 9 35 24 31 1 16 – 1 4 6 4 17 35 71 10 12 2 22 52 17 27 34 39
Mitochondria 187 328 141 162 155 287 263 – 272 254 419 355 259 309 376 4 7 2 157 223 112 79 109 113
Nucleus 0 1 0 0 2 5 3 – 2 3 8 4 0 0 2 5 1 1 0 0 0 0 0 0
Nuclear Periphery 21 16 6 5 8 18 38 – 19 17 29 34 11 15 12 0 0 1 0 1 0 4 2 2
Nucleolus 18 8 4 1 3 27 22 – 16 20 30 15 10 6 8 0 0 0 1 1 0 0 2 1
Peroxisomes 9 15 12 8 15 3 6 – 8 5 4 2 59 54 85 2 3 1 22 35 37 73 81 86
SpindlePole 1 0 4 3 6 7 16 – 9 5 8 7 6 2 4 1 0 0 0 1 0 1 0 2
Vac/Vac Membrane 22 19 4 8 18 18 61 – 13 20 17 21 13 14 23 2 3 0 3 3 1 1 2 1
Unique Cell Count 206 348 171 183 212 325 401 302 297 474 406 305 369 486 39 38 20 224 377 186 223 282 307
Labelled Cell Count 278 436 238 236 279 394 503 375 357 563 472 410 484 640 48 44 22 224 377 186 223 282 307


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 9.2 8.7 8.5 9.1 5.9 7.6 – 6.8 7.2 7.3 7.1 8.8 9.7 9.8 10.2 11.7 9.8 8.5 9.1 9.2
Std Deviation (1e-4) 1.6 1.7 1.6 1.8 2.1 1.5 1.7 – 1.8 1.7 1.9 1.7 1.6 1.8 1.9 2.9 3.1 3.3 2.4 2.3 2.5
Intensity Change (Log2) – – – -0.02 0.06 -0.56 -0.2 – -0.35 -0.28 -0.26 -0.29 0.02 0.16 0.17 0.23 0.42 0.17 -0.04 0.06 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.6 0 – 0.8 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.6 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -2.0 1.2 0 1.6 – -2.8 -2.7 -2.2 0 -0.5 -0.6 0.8 0 0 0
Golgi -1.9 -1.5 -8.2 -6.3 – -7.9 -7.2 -8.8 -8.5 -5.0 -3.5 -1.8 0.7 1.5 0
Mitochondria 1.6 -2.2 1.8 -4.1 – 2.4 0.9 2.0 1.6 0.7 0.4 -1.4 -8.8 -7.9 -7.1
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.4 0.1 1.0 2.5 – 1.3 1.1 1.3 2.1 0.1 0.3 0 0 0 0
Nucleolus 0 0 2.6 1.7 – 1.5 2.1 2.0 0.8 0.6 0 0 0 0 0
Peroxisomes -1.1 0 -3.8 -3.5 – -2.3 -3.0 0 0 3.6 2.5 3.3 0 0 0
SpindlePole 0 0 0 1.0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 2.6 1.6 4.4 – 1.1 2.1 0.8 1.5 1.1 0.9 1.4 0 0 0