Standard name
Description Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.06 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.05 0.06 0.06 0.09 0.1 0.1 0.1 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.93 0.95 0.89 0.82 0.83 0.77 0.79 0.73 0.99 0.98 0.99 0.98 0.96 0.99 0.98 0.95 0.96 0.94 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.1 0.07 0.11 0.09 0.08 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 14 20 27 9 31 0 1 0 0 0 0 0 1 0 0 0 0
Bud 0 2 2 2 3 0 0 0 4 2 0 1 0 0 0 1 0 0 1 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 4 12 5 19 18 24 36 40 43 23 49 2 2 1 5 9 2 0 0 0 0 0 0
Cytoplasm 258 345 403 317 323 306 355 344 335 339 191 238 138 177 185 250 201 247 242 333 396 77 187 196
Endoplasmic Reticulum 1 2 9 1 15 11 31 44 28 50 22 27 1 2 1 10 13 10 0 3 4 0 4 4
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 0 2 0 0 2 0 0 0 0 1 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 7 10 14 13 12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 0 2 2 5 2 2 8 0 1 1 1 0 3 0 6 4 0 0 1
Unique Cell Count 258 349 410 321 347 321 401 420 403 438 242 325 139 180 186 256 210 249 247 351 412 83 200 213
Labelled Cell Count 259 353 426 325 364 337 415 451 443 478 265 366 141 183 188 268 223 263 247 351 412 83 200 213


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 128.7 80.0 73.4 64.6 54.7 47.8 44.2 38.5 36.8 34.0 31.9 31.4 97.4 91.6 78.5 135.4 137.8 131.8 139.6 158.3 137.7
Std Deviation (1e-4) 31.6 21.2 17.7 13.9 15.9 11.7 11.8 10.1 9.6 8.8 9.3 7.8 21.2 21.0 18.2 50.2 51.1 49.2 40.6 41.8 42.5
Intensity Change (Log2) -0.18 -0.42 -0.62 -0.73 -0.93 -0.99 -1.11 -1.2 -1.22 0.41 0.32 0.1 0.88 0.91 0.84 0.93 1.11 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.7 4.6 5.1 0 6.4 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 1.8 1.8 2.1 3.5 4.1 4.1 3.6 5.9 0 0 0 -0.8 0.9 -1.8
Cytoplasm 0.5 -3.6 -2.3 -5.6 -7.9 -7.5 -9.2 -8.4 -10.1 0.8 0 1.1 -0.6 -1.9 1.0
Endoplasmic Reticulum 0 1.7 1.0 3.6 4.9 3.3 5.3 4.0 3.8 0 0 0 1.3 2.5 1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.7 0 3.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available