Standard name
Description Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.1 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0.07 0.05 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.99 0.93 0.87 0.82 0.77 0.76 0.7 0.76 0.72 0.97 0.98 0.98 0.98 0.98 0.98 0.99 0.96 0.93 0.87 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.12 0.15 0.14 0.17 0.16 0.12 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.05 0 0 0 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 5 7 8 20 6 15 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 3 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 4 1 5 8 8 17 11 18 11 6 1 1 1 0 5 1 0 0 0 0 2 1
Cytoplasm 257 409 151 110 111 148 150 196 158 139 136 157 119 171 138 131 271 294 251 390 139 109 113 114
Endoplasmic Reticulum 2 5 0 1 1 13 22 39 28 33 28 27 3 3 1 11 11 15 0 2 4 4 2 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 2 3 9 5 9 5 13 21 0 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 0 0 1 11 4 3 17 0 0 0 2 2 0 0 0 0 1 0 0
Nucleus 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 4
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 2 0 0 2 0 0 4 0 0 1 3 1 0 1 0 4 7 0 4 1 2 2 2
Unique Cell Count 259 416 151 111 119 171 182 256 207 198 179 218 123 174 141 133 277 300 255 405 150 126 125 135
Labelled Cell Count 259 419 155 112 124 174 194 269 227 219 198 246 124 175 142 146 295 318 255 405 150 126 125 135


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 116.8 76.2 93.1 83.0 52.7 48.7 42.1 39.8 36.3 36.0 34.9 32.3 89.5 85.7 80.4 92.0 128.0 120.8 88.6 105.3 96.5
Std Deviation (1e-4) 18.9 18.4 18.5 12.9 14.0 9.4 8.5 8.0 7.9 8.1 7.7 7.6 26.9 22.0 22.0 31.6 34.9 38.1 36.3 39.0 48.0
Intensity Change (Log2) – – – -0.17 -0.82 -0.94 -1.14 -1.23 -1.36 -1.37 -1.42 -1.53 -0.06 -0.12 -0.21 -0.02 0.46 0.38 -0.07 0.18 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 4.0 0 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.0 0.9 1.8 1.2 2.5 1.5 0 0 0 0 0 0 0
Cytoplasm -1.2 -3.2 -4.7 -5.4 -6.4 -6.4 -7.4 -6.5 -7.1 -2.2 -1.6 -1.8 -1.5 -1.8 -1.7
Endoplasmic Reticulum 0 0 3.5 4.4 5.0 4.7 5.3 5.1 4.5 0 0 0 3.6 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 3.4 3.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0