Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.09 0 0.08 0.07 0.06 0.1 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.14 0.43 0.23 0.29 0.38 0.48 0.48 0.53 0.56 0.58 0.28 0.18 0.2 0.45 0.43 0.43 0 0.05 0.09 0.1 0.07 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0.47 0.5 0 0.19 0.33 0.14 0.06 0 0 0 0 0.52 0.65 0.61 0.4 0.47 0.49 0.73 0.68 0.55 0.57 0.63 0.63
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0.14 0.44 0.39 0.16 0.43 0.36 0.53 0.45 0.54 0.49 0.07 0 0 0.05 0 0 0 0 0.08 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.1 0 0.07 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0 0
SpindlePole 0.32 0.15 0.22 0.32 0.33 0.19 0.18 0.12 0.11 0.09 0.08 0.14 0.26 0.27 0.19 0.19 0.2 0.1 0.08 0.1 0.08 0.1 0.06
Vac/Vac Membrane 0.11 0.32 0 0 0 0 0 0 0 0 0 0.19 0.1 0.1 0.13 0.08 0.07 0.06 0.09 0.1 0.08 0.12 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 2 1 0 2 0 0 1 3 0 0 3 4 1 0 2 3 1 1 1 1 2 1
Bud 9 6 5 19 16 41 66 34 59 32 34 6 10 9 1 4 1 2 1 0 1 4 4
Bud Neck 4 0 2 1 3 7 16 5 9 2 5 0 2 3 0 1 2 2 2 1 1 5 4
Bud Site 1 0 0 2 1 2 0 1 2 1 3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 48 35 49 48 117 200 429 267 330 223 360 90 96 126 48 46 70 17 13 13 17 38 53
Endoplasmic Reticulum 3 2 1 0 3 0 4 3 0 0 0 2 2 11 3 8 6 3 0 0 0 0 3
Endosome 185 128 0 41 132 72 57 14 21 8 4 167 355 390 42 51 80 348 204 84 102 341 442
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 1 0 4
Mitochondria 91 37 51 83 65 228 327 299 276 214 303 21 12 15 5 3 4 15 12 12 14 16 24
Nucleus 2 1 0 0 0 0 5 2 1 0 2 0 1 1 0 0 1 0 2 0 1 4 6
Nuclear Periphery 0 1 1 1 0 0 1 0 0 0 0 1 1 5 1 0 1 0 0 0 0 0 2
Nucleolus 17 5 0 2 9 1 8 0 0 0 0 4 10 15 1 2 1 4 3 1 2 6 6
Peroxisomes 9 2 0 22 18 35 20 2 11 3 4 4 0 42 0 5 0 2 2 0 1 0 1
SpindlePole 125 38 25 67 133 98 163 66 69 36 52 44 140 174 20 21 32 46 23 15 15 51 44
Vac/Vac Membrane 44 81 0 5 13 17 15 12 13 5 12 61 53 61 14 9 12 26 28 15 15 64 85
Unique Cell Count 394 256 115 212 404 528 897 561 617 395 621 322 544 639 106 108 164 480 302 153 181 545 699
Labelled Cell Count 541 338 135 291 512 701 1111 706 794 524 781 403 686 853 135 152 214 480 302 153 181 545 699


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.2 3.4 3.6 4.0 3.4 3.5 3.1 3.5 3.4 3.1 5.1 5.2 5.0 6.7 7.0 6.5 5.4 5.4 5.7
Std Deviation (1e-4) 6.5 0.8 0.7 1.0 1.1 1.3 1.1 2.8 4.1 3.8 0.5 0.9 0.8 0.8 1.2 4.5 1.6 2.8 2.5 2.5
Intensity Change (Log2) 0.09 0.26 -0.0 0.03 -0.14 0.06 0.0 -0.14 0.59 0.63 0.56 0.98 1.06 0.94 0.67 0.68 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.8 -2.8 -0.9 1.1 1.0 2.1 2.6 3.0 -2.9 -5.9 -5.4 0.4 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 9.8 12.8 12.1 7.5 8.4 8.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -9.4 -14.0 -14.7 -6.8 -7.2 -8.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 2.8 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 -2.0 0.9 1.2 -0.5 -0.4 -0.5
Vacuole 0 0 0 0 0 0 0 0 5.0 3.5 3.5 4.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3288 1.4538 1.3465 1.7106 1.5734 1.3162 0.0645 0.3297 0.2956 0.2185 0.0131 0.1563 2.2639 2.2698 2.396 2.2172 2.324 1.8245
Actin 0.007 0.0001 0.0069 0.0074 0.0276 0.0028 0.0145 0.0001 0.0048 0.004 0.0011 0.0017 0.0534 0.0001 0.0129 0.0105 0.0004 0.0001
Bud 0.0002 0.0002 0.0041 0.0001 0.0005 0.0001 0.0006 0.0001 0.0029 0.0013 0.0004 0.0001 0.0005 0.0003 0.0002 0.0006 0.0001 0.0001
Bud Neck 0.0015 0.0008 0.0022 0.0053 0.0013 0.0005 0.0006 0.0008 0.0009 0.0051 0.0002 0.001 0.0037 0.0016 0.0003 0.001 0.0009 0.0007
Bud Periphery 0.0002 0.0003 0.0011 0.0002 0.001 0.0001 0.0007 0.0001 0.0037 0.0019 0.0004 0.0001 0.0007 0.0004 0.0002 0.0008 0.0001 0.0001
Bud Site 0.0013 0.0017 0.0015 0.0009 0.0013 0.0002 0.0031 0.002 0.0091 0.0237 0.0016 0.0016 0.0104 0.003 0.0015 0.0022 0.0019 0.0004
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0002 0.0004 0.0012 0.0001 0.0001 0.0004 0.0004 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.04 0.0554 0.0675 0.0059 0.0064 0.0392 0.0855 0.0925 0.097 0.1019 0.0247 0.0319 0.071 0.1185 0.1333 0.1001 0.1765 0.122
Cytoplasmic Foci 0.234 0.3282 0.2468 0.3215 0.3324 0.3295 0.2197 0.2799 0.2125 0.1966 0.1848 0.28 0.2505 0.2414 0.2435 0.3261 0.4321 0.3087
Eisosomes 0.0001 0 0.0004 0.0001 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0061 0.0013 0.0021 0.0012 0.0053 0.0012 0.0057 0.0024 0.0067 0.0013 0.0044 0.0017 0.0067 0.0044 0.0043 0.0015 0.0002 0.0021
Endosome 0.4119 0.3848 0.391 0.398 0.2778 0.3663 0.3548 0.3739 0.3871 0.4236 0.405 0.4494 0.3079 0.2852 0.2849 0.2707 0.2719 0.3469
Golgi 0.0214 0.0204 0.0365 0.0364 0.0384 0.0215 0.0313 0.0166 0.0291 0.0331 0.0307 0.0354 0.0164 0.0108 0.0326 0.0326 0.0398 0.0212
Lipid Particles 0.1321 0.0999 0.0934 0.1129 0.0942 0.1235 0.1303 0.1023 0.073 0.0658 0.1219 0.1235 0.0858 0.076 0.0576 0.0742 0.0413 0.0848
Mitochondria 0.0102 0.0043 0.0027 0.003 0.0192 0.0012 0.0206 0.0008 0.0211 0.0454 0.0231 0.0059 0.0089 0.0047 0.0068 0.058 0.0015 0.0016
None 0.0278 0.0173 0.0571 0.0039 0.029 0.0225 0.0339 0.0685 0.0845 0.0572 0.1276 0.022 0.0904 0.1651 0.1553 0.0053 0.0004 0.0757
Nuclear Periphery 0.0129 0.0013 0.0069 0.0021 0.0317 0.0043 0.018 0.0015 0.0044 0.0007 0.0038 0.0031 0.0139 0.0027 0.004 0.0022 0.0001 0.0009
Nucleolus 0.0021 0.0059 0.0011 0.0007 0.0018 0.0005 0.0041 0.0015 0.0008 0.0007 0.0005 0.0019 0.0031 0.0055 0.0006 0.0035 0.0005 0.0005
Nucleus 0.0129 0.0021 0.0047 0.0009 0.0047 0.0008 0.0063 0.0026 0.002 0.0007 0.0009 0.0013 0.0164 0.0063 0.0043 0.0233 0.0002 0.0013
Peroxisomes 0.0485 0.0483 0.0548 0.0879 0.1064 0.0647 0.0152 0.0235 0.0241 0.0213 0.0375 0.0134 0.0216 0.0187 0.0437 0.0556 0.0275 0.0169
Punctate Nuclear 0.0147 0.0105 0.004 0.0073 0.0112 0.0121 0.0168 0.0047 0.0025 0.0007 0.0065 0.012 0.0151 0.0046 0.0042 0.0038 0.0022 0.0039
Vacuole 0.0127 0.0154 0.0129 0.0029 0.0049 0.0078 0.0301 0.0239 0.027 0.0103 0.0197 0.0105 0.0188 0.0471 0.0082 0.0232 0.0017 0.0105
Vacuole Periphery 0.0024 0.0017 0.0021 0.0011 0.0044 0.0013 0.0077 0.002 0.0064 0.0034 0.005 0.0032 0.0041 0.0031 0.0014 0.0044 0.0007 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1418 9.5765 7.1062 8.9828 2.3149 10.7067 8.8759 6.5352 12.5082 13.4791
Translational Efficiency 1.635 1.0961 1.8217 1.3226 6.8262 2.0781 1.3351 1.6037 1.5258 1.5603

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1038 1340 746 983 1323 1465 1326 826 2361 2805 2072 1809

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 632.41 758.54 918.48 837.54 671.11 762.11 873.79 797.73 654.10 760.40 889.88 819.36
Standard Deviation 71.24 107.35 105.82 114.88 93.48 100.29 100.60 108.66 86.59 103.74 104.73 113.83
Intensity Change Log 2 0.262365 0.538388 0.405298 0.183450 0.380737 0.249351 0.222275 0.459375 0.327116

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000109 0.000131 0.000299 0.000990 0.000137 0.000074 0.000222 0.000199 0.000125 0.000101 0.000250 0.000629
Bud Neck 0.055004 0.008663 0.026906 0.019487 0.024426 0.012344 0.015905 0.039007 0.037870 0.010585 0.019866 0.028400
Bud Site 0.004602 0.005555 0.010816 0.020377 0.003986 0.004552 0.010416 0.012304 0.004257 0.005031 0.010560 0.016691
Cell Periphery 0.000225 0.000024 0.000047 0.000066 0.000057 0.000065 0.000022 0.000199 0.000131 0.000046 0.000031 0.000127
Cytoplasm 0.083976 0.021568 0.012869 0.088724 0.042808 0.018431 0.014111 0.153740* 0.060907 0.019930 0.013664 0.118410*
Cytoplasmic Foci 0.248422* 0.171319 0.109335 0.183239* 0.214393* 0.145698 0.131109 0.144127 0.229354* 0.157938 0.123270 0.165380
Eisosomes 0.000279 0.000029 0.000060 0.000055 0.000099 0.000059 0.000024 0.000191 0.000178 0.000045 0.000037 0.000117
Endoplasmic Reticulum 0.000838 0.000384 0.001129 0.002053 0.000256 0.000421 0.000492 0.000950 0.000512 0.000404 0.000721 0.001549
Endosome 0.355313* 0.622060* 0.524316* 0.317248* 0.517631* 0.631759* 0.605403* 0.250417* 0.446269* 0.627126* 0.576208* 0.286733*
Golgi 0.026392 0.029505 0.007330 0.005073 0.017704 0.031620 0.006625 0.004415 0.021524 0.030610 0.006879 0.004773
Lipid Particles 0.066939 0.013257 0.009957 0.010152 0.053399 0.042180 0.006322 0.017686 0.059352 0.028363 0.007631 0.013592
Mitochondria 0.019514 0.010403 0.002326 0.001486 0.010210 0.015316 0.001943 0.007540 0.014300 0.012969 0.002081 0.004250
Mitotic Spindle 0.000380 0.021229 0.043823 0.050965 0.003112 0.007844 0.052148 0.023152 0.001911 0.014238 0.049151 0.038265
None 0.001945 0.000231 0.000242 0.001029 0.000871 0.000225 0.000145 0.004794 0.001343 0.000228 0.000180 0.002748
Nuclear Periphery 0.000860 0.000302 0.001255 0.001389 0.000390 0.000327 0.000807 0.001727 0.000597 0.000315 0.000969 0.001543
Nuclear Periphery Foci 0.000424 0.001781 0.014134 0.010379 0.001399 0.003032 0.012139 0.003223 0.000970 0.002434 0.012857 0.007111
Nucleolus 0.002583 0.001411 0.017850 0.003404 0.003149 0.002434 0.006556 0.011144 0.002900 0.001945 0.010622 0.006938
Nucleus 0.031397 0.003543 0.034542 0.024452 0.012611 0.004156 0.004814 0.128987 0.020870 0.003863 0.015517 0.072183
Peroxisomes 0.046161 0.035004 0.070049 0.065670 0.052694 0.036010 0.050748 0.032991 0.049822 0.035530 0.057697 0.050749
Vacuole 0.051095 0.048605 0.109231 0.192124 0.038699 0.038879 0.078540 0.160976 0.044149 0.043525 0.089590 0.177902
Vacuole Periphery 0.003542 0.004996 0.003484 0.001638 0.001968 0.004574 0.001509 0.002233 0.002660 0.004776 0.002220 0.001909

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.19 -4.56 -7.57 -7.27 -4.86 1.86 -2.24 -2.60 -4.45 -0.17 0.88 -4.27 -7.83 -8.33 -4.71
Bud Neck 10.74 5.43 7.28 -7.08 1.96 5.03 2.53 -2.49 -5.92 -4.32 11.67 6.36 3.56 -8.55 -2.63
Bud Site -0.81 -4.23 -7.99 -8.54 -4.53 -0.57 -7.15 -4.13 -3.57 -0.15 -1.04 -7.83 -9.07 -8.45 -3.35
Cell Periphery 9.97 8.58 7.76 -8.86 -2.80 -0.77 7.09 -6.70 -5.62 -8.79 8.19 10.23 1.47 -7.33 -9.76
Cytoplasm 11.13 12.38 -4.18 -13.93 -14.95 6.83 5.84 -13.55 -17.49 -16.97 12.69 12.79 -12.96 -22.23 -22.27
Cytoplasmic Foci 9.85 18.54 11.77 2.61 -7.33 10.34 13.40 8.35 -0.69 -3.14 14.04 21.98 14.01 1.17 -6.75
Eisosomes 13.69 11.41 11.82 -9.31 0.98 5.43 11.51 -5.81 -9.51 -12.90 14.54 15.09 6.35 -11.24 -12.19
Endoplasmic Reticulum 2.24 -2.98 -4.75 -7.54 -2.17 -2.41 -6.61 -4.62 -3.46 -1.79 1.05 -4.68 -6.95 -8.19 -3.90
Endosome -20.28 -9.26 10.61 33.69* 19.50 -9.66 -3.23 21.70 32.10 25.37* -20.03 -9.10 23.82 46.78 33.20*
Golgi -1.07 9.70 11.62 14.09 5.06 -6.35 9.06 10.43 13.95 2.72 -5.34 13.21 15.77 19.85 5.04
Lipid Particles 12.77 13.46 14.28 2.34 0.44 2.40 13.54 9.74 7.49 -4.75 9.90 19.29 17.15 8.15 -3.95
Mitochondria 4.19 9.03 9.97 7.36 1.74 -2.47 5.75 1.99 4.15 -2.32 0.86 10.55 8.04 7.57 -1.54
Mitotic Spindle -8.89 -8.94 -11.38 -6.39 -1.41 -3.37 -13.35 -6.57 -5.27 5.40 -9.12 -16.15 -12.92 -8.54 2.56
None 1.89 1.95 0.95 -2.39 -2.73 2.86 2.68 -2.51 -3.27 -3.23 2.85 2.88 -1.50 -3.84 -3.86
Nuclear Periphery 1.99 -2.63 -4.76 -10.45 -2.22 0.70 -5.87 -3.75 -3.91 -1.21 2.12 -5.23 -6.54 -8.73 -2.69
Nuclear Periphery Foci -5.26 -8.21 -7.91 -6.86 1.60 -1.86 -7.78 -4.04 -0.59 6.27 -3.21 -11.15 -8.50 -6.15 4.27
Nucleolus 2.23 -7.13 -3.20 -4.55 5.83 1.21 -4.91 -5.27 -5.98 -1.30 2.16 -8.70 -5.94 -7.32 3.69
Nucleus 10.13 -1.42 -1.65 -10.56 0.13 5.40 2.69 -14.34 -15.87 -15.19 11.26 0.84 -13.05 -18.45 -13.02
Peroxisomes 3.18 -4.18 -0.55 -3.33 3.46 4.90 1.28 5.78 1.39 4.50 5.83 -1.75 3.14 -1.91 4.49
Vacuole 0.04 -9.23 -24.56 -24.79 -14.16 0.08 -11.57 -18.39 -18.35 -10.29 -0.07 -14.51 -30.70 -30.68 -18.52
Vacuole Periphery -2.31 0.25 6.95 6.30 4.87 -6.24 2.16 0.42 5.51 -0.84 -5.75 2.05 4.75 8.60 2.77