Standard name
Human Ortholog
Description Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.15 0.13 0.22 0.11 0.14 0.17 0.16 0.1 0.07 0.07 0.13 0.31 0.21 0.27 0.15 0.13 0.16 0.14 0.09 0.09 0.24 0.18 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.32 0.37 0.58 0.6 0.5 0.75 0.78 0.74 0.77 0.83 0.81 0.82 0.08 0.12 0.06 0.12 0.08 0.07 0 0 0 0 0 0
Nucleus 0.68 0.49 0.51 0.46 0.52 0.41 0.37 0.38 0.48 0.47 0.54 0.47 0.52 0.57 0.61 0.68 0.69 0.66 0.42 0.57 0.49 0.35 0.28 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.15 0.18
Vac/Vac Membrane 0.11 0.25 0 0.05 0.05 0 0 0 0 0 0 0 0.16 0.14 0.11 0.08 0.06 0.12 0.34 0.21 0.26 0.23 0.19 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
Bud 0 0 5 2 0 2 10 7 3 3 2 1 0 0 0 1 0 1 1 3 3 2 9 9
Bud Neck 0 0 1 0 1 1 2 0 0 1 0 0 0 0 0 0 0 1 1 2 0 1 1 6
Bud Site 1 0 1 2 2 6 5 9 6 8 1 5 0 0 0 1 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 28 24 34 28 49 75 54 35 24 10 24 45 56 68 39 26 19 16 20 19 42 48 19
Endoplasmic Reticulum 0 0 1 1 0 1 1 0 0 0 0 0 0 1 1 9 8 5 0 0 2 0 2 2
Endosome 0 0 0 0 5 1 0 0 0 1 0 0 2 3 2 12 12 5 1 3 3 4 10 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 1 1 1
Mitochondria 18 70 104 93 126 267 353 247 272 302 108 147 12 32 15 32 16 9 4 6 6 6 10 6
Nucleus 38 92 91 72 133 144 167 126 170 172 72 84 76 154 151 175 138 80 50 127 105 62 74 77
Nuclear Periphery 1 0 1 1 1 3 3 2 2 3 0 0 0 0 0 7 0 0 0 0 0 0 2 1
Nucleolus 1 1 2 1 2 3 4 4 3 7 0 0 0 0 0 9 10 3 0 0 1 0 3 6
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1
SpindlePole 0 0 5 1 4 0 10 7 2 4 0 1 0 1 0 7 11 5 2 8 6 10 40 51
Vac/Vac Membrane 6 48 3 8 13 12 9 6 7 5 1 3 24 37 28 20 12 15 40 46 55 40 51 71
Unique Cell Count 56 189 179 155 254 354 453 333 351 364 134 180 147 270 248 258 201 121 120 224 214 177 269 279
Labelled Cell Count 69 239 238 215 316 489 639 462 500 530 194 265 159 285 265 314 236 143 120 224 214 177 269 279


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.8 3.6 3.5 3.5 3.3 2.7 3.2 3.0 2.9 3.0 2.7 5.1 4.3 4.7 5.3 6.3 6.1 4.1 4.6 5.1
Std Deviation (1e-4) 0.7 0.6 1.1 1.4 0.9 1.4 0.9 1.2 0.9 1.2 1.0 1.0 2.6 1.2 1.6 1.7 2.0 1.7 1.3 1.9 1.5
Intensity Change (Log2) -0.06 -0.05 -0.16 -0.43 -0.17 -0.28 -0.32 -0.3 -0.41 0.48 0.23 0.35 0.55 0.78 0.74 0.17 0.35 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.8 2.1 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0166 0.0295 0.0149 0.0052 0.0003 0.0007 0.0109 0.0005 0.0161 0.0005 0.0001 0.0002 0.0003 0.0002 0.0259 0.0004 0.0017 0.0027
Bud 0.0003 0.0001 0.0002 0.001 0 0.0001 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001 0 0.0002
Bud Neck 0.0025 0.0002 0.0075 0.002 0.0004 0.0035 0.0036 0.0005 0.0194 0.0003 0.0017 0.003 0.0002 0.0008 0.0005 0.0006 0.0006 0.0024
Bud Periphery 0.0006 0.0001 0.0003 0.0021 0 0.0001 0.0028 0.0001 0.0003 0 0.0002 0.0001 0 0.0001 0.0003 0.0001 0 0.0003
Bud Site 0.0008 0.0006 0.0088 0.0358 0.0001 0.0003 0.0023 0.0011 0.0086 0.0006 0.0001 0.0001 0.0004 0.0016 0.0019 0.0001 0.0001 0.0003
Cell Periphery 0.0003 0.0001 0.0007 0.0007 0 0 0.0014 0.0001 0.0001 0 0 0 0.0004 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.1394 0.0852 0.0816 0.0649 0.1191 0.0654 0.1113 0.1759 0.0844 0.0798 0.0175 0.0378 0.6781 0.0649 0.0844 0.1594 0.1182 0.1031
Cytoplasmic Foci 0.005 0.0016 0.0024 0.0092 0.0032 0.002 0.0143 0.004 0.0152 0.0222 0.0004 0.0012 0.0046 0.0038 0.0415 0.0049 0.0072 0.0038
Eisosomes 0.0013 0.0002 0.0003 0.0004 0.0001 0 0.0002 0 0.0001 0 0 0 0 0 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0085 0.0026 0.0022 0.0021 0.0049 0.0018 0.0103 0.0052 0.0042 0.0011 0.0005 0.001 0.0074 0.003 0.0044 0.0212 0.0074 0.0036
Endosome 0.0485 0.0022 0.0053 0.0063 0.0049 0.0031 0.0484 0.004 0.0202 0.0242 0.0003 0.0013 0.0007 0.0208 0.0318 0.0064 0.0117 0.0082
Golgi 0.0043 0.0019 0.0029 0.0025 0.0001 0.0001 0.0017 0.0002 0.0047 0.018 0 0 0.0001 0.0009 0.0365 0.0001 0.0005 0.0013
Lipid Particles 0.0084 0.0021 0.007 0.0079 0.0011 0.0003 0.0039 0.0005 0.0024 0.0084 0 0.0003 0.0002 0.0031 0.0295 0.0004 0.0004 0.0013
Mitochondria 0.0053 0.0014 0.0021 0.0079 0.0004 0.0004 0.0062 0.0007 0.0093 0.0032 0.0003 0.0005 0.0001 0.0005 0.0517 0.0009 0.0006 0.001
None 0.2702 0.1832 0.1593 0.1098 0.2451 0.0868 0.2796 0.2145 0.2417 0.3163 0.4137 0.2011 0.3018 0.27 0.1991 0.0878 0.5662 0.0894
Nuclear Periphery 0.0546 0.0329 0.0122 0.0121 0.0396 0.0123 0.053 0.0129 0.0202 0.0041 0.0023 0.0074 0.0011 0.0266 0.0124 0.0556 0.0169 0.0155
Nucleolus 0.0108 0.0037 0.0055 0.0077 0.0062 0.0091 0.0113 0.0033 0.0024 0.0035 0.0025 0.0094 0.0001 0.0054 0.0044 0.0042 0.0045 0.0061
Nucleus 0.3865 0.6346 0.6664 0.6807 0.5104 0.776 0.3659 0.5352 0.5168 0.4973 0.5575 0.7041 0.0024 0.576 0.4288 0.5907 0.249 0.7375
Peroxisomes 0.0021 0.0025 0.0018 0.005 0.0001 0.0001 0.0209 0.0001 0.0064 0.0061 0 0.0001 0 0.0002 0.0267 0.0001 0.0002 0.0008
Punctate Nuclear 0.0257 0.0103 0.0167 0.0341 0.0611 0.0363 0.0241 0.0378 0.0225 0.0131 0.0015 0.031 0.0009 0.0166 0.0155 0.0631 0.0138 0.02
Vacuole 0.0039 0.0027 0.0013 0.0021 0.0024 0.0013 0.0222 0.0029 0.0038 0.0008 0.0012 0.001 0.0011 0.0043 0.0028 0.0029 0.0004 0.002
Vacuole Periphery 0.0043 0.0021 0.0004 0.0004 0.0005 0.0003 0.0051 0.0004 0.0007 0.0003 0.0001 0.0002 0 0.001 0.0009 0.0009 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.4292 4.9723 5.5345 6.996 5.4087 12.5081 9.5836 12.9366 9.9631 10.7936
Translational Efficiency 1.1969 1.3685 0.7544 0.7491 1.2797 1.246 1.2488 0.8183 1.1481 0.8489

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
767 1124 330 1300 1578 1690 123 421 2345 2814 453 1721

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 710.98 678.71 988.88 1043.25 688.51 737.57 1039.43 1103.75 695.86 714.06 1002.61 1058.05
Standard Deviation 76.81 112.69 129.91 157.81 74.17 103.62 116.14 167.84 75.78 111.14 128.31 162.42
Intensity Change Log 2 -0.067014 0.475986 0.553204 0.099302 0.594243 0.680864 0.017205 0.535377 0.617421

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000026 0.000456 0.001163 0.001333 0.000058 0.000236 0.001215 0.001519 0.000047 0.000324 0.001177 0.001379
Bud Neck 0.004343 0.009477 0.007450 0.025518 0.002222 0.007392 0.010065 0.024109 0.002915 0.008225 0.008160 0.025173
Bud Site 0.001140 0.003804 0.001879 0.013127 0.000473 0.001776 0.004156 0.012788 0.000691 0.002586 0.002497 0.013044
Cell Periphery 0.000149 0.000267 0.000085 0.000150 0.000126 0.000132 0.000103 0.000191 0.000134 0.000186 0.000090 0.000160
Cytoplasm 0.241971* 0.167032* 0.002347 0.007052 0.198534* 0.119311* 0.002443 0.006679 0.212741* 0.138372* 0.002373 0.006960
Cytoplasmic Foci 0.014491 0.027380 0.000165 0.004971 0.035906 0.022119 0.000087 0.013559 0.028901 0.024220 0.000143 0.007072
Eisosomes 0.000021 0.000044 0.000047 0.000040 0.000041 0.000036 0.000046 0.000048 0.000034 0.000039 0.000047 0.000042
Endoplasmic Reticulum 0.000751 0.003379 0.009774 0.006397 0.000795 0.002287 0.009614 0.009009 0.000781 0.002723 0.009730 0.007036
Endosome 0.000798 0.003532 0.000368 0.002297 0.001513 0.001747 0.000410 0.016916 0.001279 0.002460 0.000379 0.005873
Golgi 0.000084 0.001246 0.000079 0.003316 0.000220 0.000759 0.000134 0.018576 0.000175 0.000953 0.000094 0.007049
Lipid Particles 0.001307 0.004125 0.000433 0.000385 0.004867 0.003495 0.000266 0.001299 0.003703 0.003746 0.000387 0.000609
Mitochondria 0.001858 0.007002 0.001044 0.007378 0.002914 0.003731 0.001958 0.011654 0.002569 0.005038 0.001292 0.008424
Mitotic Spindle 0.001074 0.004966 0.000370 0.188621* 0.002524 0.003713 0.020997 0.236031* 0.002049 0.004213 0.005971 0.200218*
None 0.005898 0.023602 0.002101 0.008388 0.006581 0.009646 0.004722 0.006480 0.006358 0.015220 0.002812 0.007922
Nuclear Periphery 0.001203 0.001837 0.000669 0.002622 0.001593 0.002105 0.000737 0.003630 0.001465 0.001998 0.000687 0.002869
Nuclear Periphery Foci 0.000157 0.000927 0.000193 0.002657 0.000355 0.000886 0.002779 0.010077 0.000290 0.000902 0.000895 0.004472
Nucleolus 0.001984 0.013088 0.006721 0.074118 0.002388 0.005947 0.012049 0.057406 0.002256 0.008799 0.008168 0.070030
Nucleus 0.717408* 0.711638* 0.960205* 0.639673* 0.732094* 0.804976* 0.923306* 0.523519* 0.727290* 0.767694* 0.950186* 0.611258*
Peroxisomes 0.000691 0.005045 0.000331 0.002934 0.001217 0.002959 0.000615 0.005235 0.001045 0.003792 0.000408 0.003497
Vacuole 0.004502 0.009987 0.004430 0.007356 0.005223 0.006284 0.004105 0.024541 0.004987 0.007763 0.004341 0.011560
Vacuole Periphery 0.000144 0.001167 0.000149 0.001667 0.000358 0.000464 0.000194 0.016735 0.000288 0.000745 0.000161 0.005353

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.72 -15.29 -20.55 -11.16 -3.20 -3.67 -9.29 -14.01 -11.95 -0.96 -6.47 -17.30 -24.17 -17.53 -3.08
Bud Neck -3.96 -2.70 -18.58 -16.86 -17.23 -5.56 -4.57 -14.35 -11.64 -5.45 -7.26 -6.36 -24.88 -20.59 -17.12
Bud Site -3.84 -1.27 -13.20 -10.37 -10.07 -4.49 -3.15 -7.73 -7.25 -5.45 -5.88 -2.98 -15.56 -13.68 -11.37
Cell Periphery -3.72 2.94 -2.22 2.85 -5.67 -0.54 -0.15 -3.91 -3.31 -2.27 -3.65 2.45 -5.54 0.11 -6.03
Cytoplasm 7.38 28.72 28.34 23.90 -2.86 11.51 35.96 35.62 26.16 -2.00 12.91 45.95 45.19 35.02 -3.34
Cytoplasmic Foci -4.50 7.74 4.64 9.15 -6.10 5.17 17.58 9.25 5.24 -4.72 2.14 19.04 13.43 12.22 -7.70
Eisosomes -7.85 -8.00 -14.42 -5.34 -2.45 0.79 -3.34 -7.19 -7.56 -1.03 -3.41 -6.34 -13.47 -9.93 -2.49
Endoplasmic Reticulum -8.94 -14.60 -11.13 -3.66 7.87 -10.09 -9.12 -8.57 -6.09 3.37 -13.76 -17.70 -15.33 -7.97 8.51
Endosome -4.88 1.74 -2.48 3.93 -6.30 -0.88 3.71 -2.00 -1.80 -2.69 -4.06 4.84 -2.05 1.01 -4.56
Golgi -3.32 -0.64 -4.43 -1.26 -4.40 -2.01 -1.87 -2.66 -2.33 -2.56 -3.64 0.02 -4.63 -2.97 -4.63
Lipid Particles -6.12 3.58 3.65 8.74 0.32 2.26 8.75 7.93 8.00 -1.81 -0.13 9.21 8.99 12.44 -0.71
Mitochondria -3.81 0.76 -2.53 1.80 -4.83 -0.92 1.12 -1.52 -1.00 -2.47 -3.14 1.94 -2.60 0.30 -5.37
Mitotic Spindle -3.28 -0.69 -23.95 -23.07* -22.81 -1.34 -2.99 -13.85 -13.72 -8.58 -2.92 -2.75 -27.56 -27.07 -22.72
None -6.89 3.99 0.42 7.21 -3.82 -2.20 1.23 1.33 3.13 -0.49 -6.64 5.06 1.22 7.20 -3.42
Nuclear Periphery -3.52 2.65 -7.29 -3.83 -11.06 -3.47 2.22 -3.11 -1.59 -4.06 -4.64 5.26 -7.27 -3.84 -11.26
Nuclear Periphery Foci -4.29 -1.24 -9.55 -7.23 -8.58 -1.26 -1.45 -3.41 -2.89 0.55 -2.35 -1.44 -8.95 -7.18 -1.90
Nucleolus -6.53 -2.55 -27.10* -24.15* -24.75* -4.72 -3.87 -14.38 -13.82 -8.92 -8.03 -4.48 -30.67* -28.74* -25.27*
Nucleus 0.77 -21.94 16.34 15.78 39.80 -7.56 -5.13 13.21 16.83 13.00 -4.79 -21.54 22.79 26.37 37.36
Peroxisomes -4.74 1.50 -11.59 -1.02 -13.80 -4.50 0.19 -6.79 -5.11 -6.74 -6.39 3.14 -12.87 -5.03 -14.19
Vacuole -4.16 -1.41 -2.51 1.92 -1.46 -1.93 -1.55 -3.88 -3.12 -2.28 -4.45 -1.79 -4.08 -0.65 -2.44
Vacuole Periphery -3.33 -0.82 -4.15 1.14 -4.04 -0.77 1.22 -2.27 -2.20 -2.36 -2.99 1.54 -3.07 -1.88 -3.39