Standard name
Human Ortholog
Description Forkhead family transcription factor; rate-limiting activator of replication origins; evolutionarily conserved regulator of lifespan; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; positively regulates transcriptional elongation; facilitates clustering, activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; major role in expression of G2/M phase genes; relocalizes to cytosol under hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.15 0 0 0 0.07 0.06 – 0 0 0.06 0 0 0.08 0.12 0.1 0.08 0.09 0 0.07 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.6 0 0.08 0 0.21 0.23 – 0.42 0.41 0.55 0.3 0.1 0 0.07 0.09 0 0.1 0 0 0 0 0 0
Nucleus 0.87 0.61 0.94 0.93 0.89 0.77 0.81 – 0.76 0.76 0.68 0.79 0.91 0.85 0.78 0.8 0.79 0.81 0.8 0.63 0.82 0.74 0.58 0.49
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0.05 0 0 0.05 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.1 0.09 0 0.18 0.09 0.13 0.2 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0 4 0
Bud 0 4 0 1 2 3 1 – 2 4 7 8 0 0 0 1 0 0 1 8 2 4 11 9
Bud Neck 0 0 0 0 5 2 1 – 0 2 0 2 0 0 0 0 1 0 1 4 1 1 2 5
Bud Site 0 4 0 0 0 5 23 – 17 25 24 21 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 18 56 15 13 22 34 40 – 24 19 21 18 20 43 53 37 33 27 2 26 4 2 6 7
Endoplasmic Reticulum 3 0 0 0 0 0 0 – 0 0 0 1 0 0 1 12 16 4 1 0 0 2 3 3
Endosome 0 0 2 4 4 11 10 – 3 4 3 6 0 7 6 5 9 4 4 1 3 1 10 13
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 3 0 0 0 3 0
Mitochondria 21 216 8 29 19 109 158 – 253 264 211 155 48 22 33 32 13 32 4 7 9 1 10 16
Nucleus 242 220 427 343 441 392 557 – 456 485 259 404 425 434 354 289 310 249 235 246 404 212 289 200
Nuclear Periphery 1 2 0 2 0 0 0 – 3 6 2 5 2 5 3 1 0 0 3 0 1 0 1 3
Nucleolus 9 6 0 0 1 5 6 – 17 26 25 19 9 16 16 5 5 1 15 3 10 13 32 24
Peroxisomes 0 0 0 1 0 0 0 – 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0
SpindlePole 2 5 19 5 17 13 25 – 10 13 10 17 0 8 3 2 5 0 3 12 5 6 12 16
Vac/Vac Membrane 7 12 7 5 14 19 22 – 12 15 8 12 6 14 14 27 40 29 12 70 42 35 99 92
Unique Cell Count 279 363 454 370 497 507 690 599 641 381 513 466 513 453 362 394 308 295 389 491 285 496 405
Labelled Cell Count 303 525 478 403 526 593 843 797 863 570 668 510 550 484 412 435 348 295 389 491 285 496 405


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 3.5 5.9 5.4 5.8 4.7 5.0 – 4.5 4.5 4.1 4.9 5.3 5.3 5.6 7.8 8.0 8.0 4.9 4.9 5.1
Std Deviation (1e-4) 1.1 0.7 1.1 1.1 1.2 1.0 1.3 – 1.0 1.1 1.2 1.0 1.1 1.1 7.0 1.9 1.9 2.0 1.0 1.1 1.4
Intensity Change (Log2) – – – -0.13 -0.02 -0.34 -0.23 – -0.39 -0.38 -0.51 -0.28 -0.16 -0.16 -0.08 0.4 0.44 0.45 -0.27 -0.27 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.2 5.5 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6962 3.5652 3.6613 3.5041 3.1036 3.5017 1.1254 1.3544 1.3582 1.2168 1.4002 1.1061 1.3706 1.6548 1.5864 1.4466 1.3971 1.389
Actin 0.024 0 0 0 0.0004 0 0.0045 0 0 0 0.0238 0.0006 0.0014 0 0 0 0 0
Bud 0.0002 0 0 0 0.0001 0 0.0005 0.0003 0 0.0003 0.0002 0.0001 0.0001 0 0 0 0.0001 0
Bud Neck 0.0016 0.0001 0.0001 0.0002 0.0013 0.0009 0.0013 0.0002 0.0001 0.0007 0.0008 0.0008 0.0004 0.0001 0 0 0.0004 0.0006
Bud Periphery 0.0004 0 0 0.0001 0.0001 0 0.0011 0.0004 0 0.0008 0.0002 0.0003 0.0001 0 0 0 0.0002 0
Bud Site 0.0006 0.0001 0 0.0001 0.0003 0.0001 0.0035 0.0011 0.0001 0.0002 0.0016 0.0001 0.0007 0.0001 0 0 0.0003 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0004 0.0001 0 0.0001 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0042 0.0004 0.0001 0.0006 0.001 0 0.0011 0.0005 0.0005 0.0005 0.0003 0.0002 0.0012 0.0004 0.0001 0 0.0006 0.0022
Cytoplasmic Foci 0.005 0 0 0.0034 0.0001 0 0.0135 0.0001 0.0028 0 0.0025 0.0012 0.0012 0 0 0 0 0.0003
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0013 0 0 0.0001 0.0011 0 0.0008 0 0.0002 0.0003 0 0.0003 0.0003 0 0 0 0.0001 0
Endosome 0.0044 0 0 0.0007 0.0013 0 0.016 0.0001 0.007 0.0014 0.0002 0.0029 0.0015 0.0001 0 0 0.0002 0.0001
Golgi 0.0015 0 0 0.0001 0 0 0.007 0 0.0027 0 0.0007 0.0019 0.0004 0 0 0 0 0
Lipid Particles 0.0014 0 0 0.0003 0.0001 0 0.0143 0 0.0007 0 0.0005 0.0014 0.0005 0 0 0 0 0
Mitochondria 0.0023 0.0001 0.0001 0.0004 0.0008 0 0.0024 0.0002 0.0004 0.0006 0.0008 0.0013 0.0008 0.0001 0.0001 0 0.0005 0.0001
None 0.0023 0.0086 0.0001 0.0008 0.0003 0 0.0023 0.0002 0.0001 0 0.0002 0 0.0028 0.0001 0 0 0.0001 0.0002
Nuclear Periphery 0.0184 0.001 0.0014 0.0017 0.0306 0.0002 0.0016 0.0051 0.0029 0.0017 0.0024 0.0029 0.0014 0.0053 0.0007 0.0003 0.0063 0.0008
Nucleolus 0.0149 0.0162 0.0103 0.0076 0.0466 0.0399 0.0231 0.0155 0.0054 0.0178 0.0566 0.0312 0.0098 0.009 0.0061 0.003 0.0677 0.0294
Nucleus 0.9018 0.9718 0.9868 0.9407 0.9089 0.9576 0.893 0.9646 0.972 0.9407 0.8904 0.9469 0.9626 0.9794 0.9828 0.9964 0.9046 0.96
Peroxisomes 0.0009 0 0 0.0001 0 0 0.0048 0 0.0001 0 0.0157 0.0055 0.0001 0 0 0 0 0
Punctate Nuclear 0.0129 0.0014 0.0011 0.0425 0.0058 0.0011 0.004 0.0107 0.0047 0.0072 0.0028 0.0006 0.014 0.0053 0.01 0.0002 0.0187 0.0059
Vacuole 0.001 0.0002 0 0.0004 0.0006 0.0001 0.0038 0.0007 0.0002 0.0205 0.0001 0.001 0.0004 0.0001 0 0 0.0001 0.0001
Vacuole Periphery 0.0007 0 0 0.0003 0.0006 0 0.0008 0.0001 0.0001 0.0074 0.0001 0.0008 0.0002 0.0001 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.294 11.0486 9.3294 13.1715 12.7092 8.945 18.0088 21.7096 12.4858 17.4016
Translational Efficiency 1.1389 1.7305 1.3525 0.6001 0.9677 1.1875 0.9685 0.6221 0.8333 0.7415

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1061 1928 156 1381 1932 1760 105 827 2993 3688 261 2208

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 640.71 702.83 1045.15 952.02 747.84 759.43 1071.71 941.74 709.86 729.84 1055.84 948.17
Standard Deviation 84.95 132.82 121.78 134.37 87.58 115.52 120.30 145.58 100.68 128.02 121.89 138.76
Intensity Change Log 2 — 0.133504 0.705967 0.571320 — 0.022187 0.519113 0.332599 — 0.074621 0.608347 0.447674

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000118 0.000353 0.001481 0.002944 0.000109 0.000352 0.002198 0.001031 0.000112 0.000353 0.001770 0.002228
Bud Neck 0.003242 0.009067 0.014855 0.021004 0.003606 0.007818 0.006391 0.020317 0.003477 0.008471 0.011450 0.020747
Bud Site 0.001041 0.002060 0.002218 0.026653 0.000553 0.001821 0.001754 0.010242 0.000726 0.001946 0.002031 0.020506
Cell Periphery 0.000147 0.000227 0.000077 0.000390 0.000057 0.000129 0.000108 0.000216 0.000089 0.000180 0.000090 0.000325
Cytoplasm 0.046342 0.039465 0.001369 0.062376 0.016356 0.018257 0.002917 0.023894 0.026986 0.029344 0.001992 0.047962
Cytoplasmic Foci 0.006903 0.002582 0.000085 0.001660 0.001970 0.003832 0.000447 0.000232 0.003719 0.003178 0.000231 0.001125
Eisosomes 0.000022 0.000019 0.000049 0.000029 0.000013 0.000026 0.000096 0.000030 0.000016 0.000022 0.000068 0.000030
Endoplasmic Reticulum 0.001195 0.003282 0.008862 0.007326 0.001744 0.004137 0.017981 0.006632 0.001549 0.003690 0.012531 0.007066
Endosome 0.000774 0.000712 0.000365 0.002733 0.000486 0.001809 0.000634 0.000487 0.000588 0.001236 0.000473 0.001892
Golgi 0.000333 0.000144 0.000114 0.002313 0.000277 0.001063 0.000116 0.000139 0.000297 0.000583 0.000115 0.001499
Lipid Particles 0.001076 0.000637 0.000322 0.000547 0.000631 0.001739 0.001285 0.000274 0.000789 0.001163 0.000709 0.000445
Mitochondria 0.001745 0.001650 0.001497 0.004476 0.001185 0.003884 0.001298 0.002532 0.001383 0.002716 0.001417 0.003748
Mitotic Spindle 0.001821 0.003567 0.000600 0.037500 0.001974 0.005050 0.000312 0.011133 0.001920 0.004275 0.000484 0.027624
None 0.024769 0.022918 0.002600 0.008481 0.004732 0.008234 0.001500 0.016307 0.011835 0.015910 0.002158 0.011412
Nuclear Periphery 0.000859 0.001156 0.000585 0.001876 0.001626 0.001754 0.001153 0.001166 0.001354 0.001442 0.000813 0.001610
Nuclear Periphery Foci 0.000484 0.000934 0.000204 0.000617 0.000333 0.001128 0.000394 0.000436 0.000386 0.001027 0.000281 0.000549
Nucleolus 0.017146 0.013959 0.008039 0.001364 0.011350 0.016946 0.004782 0.003620 0.013404 0.015385 0.006729 0.002209
Nucleus 0.890244* 0.895530* 0.950398* 0.798292* 0.951155* 0.918244* 0.937710* 0.894393* 0.929562* 0.906370* 0.945293* 0.834287*
Peroxisomes 0.000693 0.000441 0.000429 0.000321 0.000285 0.000967 0.000545 0.000179 0.000430 0.000692 0.000475 0.000268
Vacuole 0.000854 0.001027 0.005674 0.016487 0.001335 0.002222 0.018192 0.006331 0.001165 0.001597 0.010710 0.012683
Vacuole Periphery 0.000192 0.000266 0.000177 0.002612 0.000224 0.000587 0.000186 0.000409 0.000213 0.000420 0.000180 0.001787

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.77 -9.74 -5.11 -4.29 0.54 -6.69 -11.36 -14.59 -11.44 3.87 -10.54 -14.49 -7.63 -6.35 1.86
Bud Neck -5.46 -7.17 -13.90 -9.79 -1.11 -4.48 -5.66 -12.33 -9.34 -8.20 -7.50 -8.46 -19.38 -13.64 -4.85
Bud Site -1.94 -3.74 -7.26 -6.68 -6.01 -3.89 -5.81 -5.05 -4.35 -3.68 -4.24 -7.07 -9.05 -8.01 -7.05
Cell Periphery -2.34 2.04 -1.95 -0.11 -3.15 -5.63 -5.08 -6.74 -2.48 -2.82 -5.31 -2.04 -4.81 -1.14 -3.86
Cytoplasm 1.74 12.34 0.42 -1.67 -17.55 -1.10 7.28 -1.18 -0.33 -6.30 -1.02 14.65 -3.46 -2.61 -17.99
Cytoplasmic Foci 3.04 5.09 4.65 3.87 -3.65 -2.50 3.77 4.07 6.78 0.49 0.96 6.41 5.77 7.24 -2.93
Eisosomes 2.00 -6.14 -9.57 -13.38 2.81 -7.31 -9.38 -14.24 -6.51 5.80 -5.39 -11.24 -18.96 -13.33 5.96
Endoplasmic Reticulum -10.91 -7.43 -20.38 -14.30 0.33 -6.42 -10.53 -16.89 -8.93 5.76 -10.42 -12.03 -25.67 -16.04 3.94
Endosome 0.50 1.25 -1.99 -3.27 -4.00 -3.56 -2.26 -2.25 3.04 0.93 -3.18 -0.45 -3.52 0.59 -3.31
Golgi 1.45 1.27 -1.09 -2.05 -1.95 -1.61 0.59 0.58 2.05 -0.10 -1.10 0.97 -0.85 0.35 -1.95
Lipid Particles 2.49 3.53 1.96 -1.02 -2.62 -3.40 -1.50 1.50 4.40 2.57 -2.05 0.70 1.47 3.41 0.47
Mitochondria 0.05 -0.15 -1.73 -4.34 -3.66 -4.54 -1.16 -4.60 1.52 -4.99 -3.09 -0.92 -4.30 -1.22 -5.47
Mitotic Spindle -1.68 0.39 -6.51 -5.80 -6.82 -2.21 -0.59 -4.33 -3.13 -3.00 -2.81 -0.32 -7.88 -6.49 -7.09
None 0.97 9.06 7.53 8.51 -5.95 -3.61 4.27 -2.40 -0.44 -3.95 -2.58 9.61 3.75 6.39 -6.55
Nuclear Periphery -2.18 0.10 -12.50 -10.60 -12.16 -0.35 -0.01 -1.96 -2.01 -2.60 -0.42 0.78 -7.11 -8.90 -10.99
Nuclear Periphery Foci -2.71 0.89 -2.07 1.16 -2.77 -3.48 -1.15 -2.54 2.48 0.02 -4.68 -0.39 -3.82 2.55 -2.02
Nucleolus 2.38 3.27 11.37 13.56 4.58 -4.37 5.97 7.37 9.56 -0.19 -2.05 4.15 14.80 16.29 4.27
Nucleus -1.07 -5.35 7.67 10.05 13.40 6.84 3.38 10.85 5.74 5.15 5.44 -0.13 16.26 12.01 13.76
Peroxisomes 0.97 1.32 2.79 3.31 4.58 -4.65 -1.02 1.61 6.54 3.96 -2.33 0.25 3.24 7.03 5.97
Vacuole -0.43 -3.21 -14.09 -14.06 -8.47 -1.61 -4.80 -7.48 -7.24 -0.59 -1.20 -5.57 -15.76 -15.65 -6.88
Vacuole Periphery -1.00 -0.63 -1.59 -1.39 -1.53 -1.95 0.53 -0.78 1.49 -1.28 -2.09 0.10 -1.65 -0.74 -1.68
Standard name
Human Ortholog
Description Forkhead family transcription factor; rate-limiting activator of replication origins; evolutionarily conserved regulator of lifespan; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; positively regulates transcriptional elongation; facilitates clustering, activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; major role in expression of G2/M phase genes; relocalizes to cytosol under hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Fkh2

Fkh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fkh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available