Standard name
Description Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; acts as an adaptor that helps Rsp5p recognize cytosolic misfolded proteins for ubiquitination after heat shock; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family; chimeric protein in which human p58IPK J domain replaces yeast Ydj1p J domain can complement yeast ydj1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm – 0.91 0.85 0.83 0.55 0.69 0.8 0.82 0.69 0.78 0.88 0.66 0.84 0.86 0.77 – – – 0.81 0.58 0.8 0.77 0.69 0.63
Endoplasmic Reticulum – 0 0.06 0 0.21 0 0.09 0 0.1 0 0 0.09 0.07 0 0.12 – – – 0 0 0 0 0 0.05
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0.2 0.07 0.07 0.15 0.09
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0.06 0.05 0.07 0.12 0.06 0.1 0.1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0.05 0 0.13 0.21 0.08 0 0.05 0 0 0 0.07 0.08 0.09 0.11 – – – 0.06 0.09 0 0.05 0.07 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 1 1 0 0 0 0 1 0 0 0 – – – 0 1 1 0 0 0
Bud – 0 2 0 0 1 0 0 0 0 0 1 0 1 0 – – – 0 4 1 6 3 8
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 2 0 1
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 2 0 1 0 2 1 0 2 2 2 3 0 2 3 – – – 0 0 0 0 0 0
Cytoplasm – 77 89 38 16 25 63 68 34 25 46 46 74 84 83 – – – 36 84 116 120 106 122
Endoplasmic Reticulum – 3 6 1 6 1 7 3 5 1 1 6 6 2 13 – – – 0 1 3 3 4 9
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 4 1 1 0 0 0 0 2 0 1 1 1 1 – – – 0 0 0 0 0 0
Mitochondria – 0 4 0 0 2 2 1 2 0 3 3 0 0 0 – – – 0 1 2 0 1 0
Nucleus – 0 0 0 0 0 0 1 0 0 1 1 0 0 0 – – – 2 29 10 11 23 18
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 0 0 0 0
Nucleolus – 1 1 0 0 2 4 6 6 2 5 7 0 1 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 4 4 6 6 3 1 4 2 0 0 5 7 9 12 – – – 2 12 4 8 11 26
Unique Cell Count 85 105 46 29 36 79 83 49 32 52 70 88 98 108 45 145 145 156 156 196
Labelled Cell Count 87 110 47 29 37 79 83 51 32 58 74 88 100 112 45 145 145 156 156 196


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 85.4 80.8 66.2 65.4 61.5 60.9 59.9 64.5 63.9 54.1 63.9 87.2 88.5 93.5 – – – 97.2 97.1 100.6
Std Deviation (1e-4) – 36.0 30.6 28.7 31.3 31.0 31.1 31.7 32.9 31.1 32.2 31.4 29.6 28.3 28.8 – – – 33.2 28.9 36.9
Intensity Change (Log2) – – – -0.29 -0.3 -0.39 -0.41 -0.43 -0.32 -0.34 -0.58 -0.34 0.11 0.13 0.21 – – – 0.27 0.27 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm -0.3 -3.4 -2.0 -0.9 -0.5 -2.2 -0.9 0.6 -2.9 -0.1 0.2 -1.5 – – –
Endoplasmic Reticulum 0 0 0 0.8 0 0 0 0 0 0.3 0 1.6 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 1.2 1.6 2.0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.614 99.2428 89.8707 81.2459 70.0218 90.8773 90.1076 122.9645 111.4219 106.9378 98.0775 113.1206 48.5757 125.0023 109.1838 115.1734 98.5834 118.8326
Actin 0.0343 0.0001 0.0062 0.0013 0.0339 0.0001 0.0005 0.0001 0.0019 0 0.0025 0.0001 0.0233 0.0002 0.0007 0.0017 0.0041 0.0002
Bud 0.0036 0.0003 0.0007 0.0027 0.0031 0.0001 0.0023 0 0.0001 0 0.0003 0.0001 0.0035 0 0.0014 0 0.0121 0.0002
Bud Neck 0.0025 0.0001 0.0003 0.001 0.0004 0.0005 0.0003 0.0001 0.0001 0.0001 0.0004 0.0003 0.0022 0.0001 0.0002 0.0001 0.0003 0.0004
Bud Periphery 0.0018 0.0001 0.0003 0.0019 0.004 0.0001 0.0016 0 0 0 0.0002 0 0.003 0 0.0005 0 0.0105 0
Bud Site 0.0085 0.0002 0.0007 0.0084 0.0007 0 0.0008 0 0.0001 0 0.0003 0 0.0282 0 0.001 0.0001 0.0047 0
Cell Periphery 0.003 0.0001 0.0004 0.0005 0.0005 0.0035 0.0016 0.0001 0.0001 0 0.0008 0 0.0018 0 0.0006 0 0.0005 0.0001
Cytoplasm 0.4084 0.9755 0.8948 0.8038 0.7527 0.9199 0.8747 0.9857 0.9643 0.9478 0.8748 0.9534 0.5379 0.9911 0.8763 0.9142 0.8437 0.9444
Cytoplasmic Foci 0.0954 0.0034 0.0117 0.0242 0.0275 0.0003 0.0101 0.0001 0.0008 0 0.0074 0.0001 0.0702 0.0001 0.0093 0.0097 0.0019 0.0003
Eisosomes 0.0011 0 0.0001 0 0.0004 0 0.0002 0 0 0 0.0001 0 0.0003 0 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.006 0.0001 0.0013 0.0004 0.0021 0.0009 0.0011 0.0001 0.0006 0.0001 0.001 0.0004 0.0049 0 0.0005 0.0006 0.0152 0.0001
Endosome 0.0393 0.0001 0.009 0.0117 0.0101 0.0001 0.0015 0 0.0009 0 0.0037 0.0001 0.0211 0 0.0036 0.0024 0.021 0
Golgi 0.0222 0 0.0088 0.0055 0.0054 0 0.0002 0 0.0003 0 0.0011 0 0.0109 0 0.0008 0.0009 0.0015 0
Lipid Particles 0.0388 0.0001 0.0059 0.0055 0.0092 0.0004 0.002 0 0.0003 0 0.0047 0 0.0563 0 0.0025 0.0074 0.0004 0.0001
Mitochondria 0.0212 0.0004 0.0071 0.0172 0.0712 0.0002 0.0074 0 0.0006 0 0.0027 0.0001 0.0204 0.0001 0.0183 0.0013 0.0028 0.0003
None 0.051 0.0024 0.0043 0.0012 0.0034 0.0003 0.0091 0.0001 0.0009 0 0.0083 0.0001 0.0296 0.0001 0.0067 0.0048 0.0013 0.0003
Nuclear Periphery 0.029 0.0018 0.0036 0.0061 0.0109 0.0132 0.0087 0.0009 0.0033 0.0044 0.0212 0.0093 0.0195 0.0008 0.0084 0.007 0.0243 0.0041
Nucleolus 0.0397 0.0006 0.0038 0.0046 0.0022 0.0073 0.0146 0 0.0015 0 0.0105 0 0.0255 0 0.0098 0.0005 0.0003 0.0001
Nucleus 0.1017 0.0125 0.0317 0.0781 0.0295 0.0481 0.0366 0.0124 0.0214 0.0473 0.04 0.0355 0.0513 0.0073 0.0465 0.0328 0.015 0.0485
Peroxisomes 0.0273 0 0.002 0.0028 0.0032 0 0.0004 0 0.0001 0 0.002 0 0.0453 0 0.0015 0.0013 0.0002 0
Punctate Nuclear 0.0275 0.0005 0.0005 0.0008 0.0016 0.0002 0.0101 0 0.0003 0.0001 0.0122 0.0001 0.0213 0.0001 0.0027 0.0145 0.0007 0.0003
Vacuole 0.0261 0.0011 0.005 0.0126 0.0207 0.0037 0.012 0.0002 0.0011 0.0001 0.0036 0.0004 0.0155 0 0.0044 0.0002 0.0299 0.0003
Vacuole Periphery 0.0114 0.0005 0.0018 0.0096 0.0075 0.0009 0.0042 0 0.0014 0.0001 0.0022 0.0002 0.0079 0 0.0041 0.0004 0.0095 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 289.3073 392.1447 375.4879 384.3145 374.518 257.3185 414.1327 449.0246 405.7855 332.6592
Translational Efficiency 3.1013 3.3163 2.6705 2.5379 2.5856 3.0338 2.5955 2.3446 2.3168 2.4116

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘