Standard name
Human Ortholog
Description rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 – 0 0 0 0 0.06 0.1 0.06 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.06 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.09 0 0.23 0 0.14 0.09 – 0.13 0.11 0.26 0.08 0.25 0.14 0.07 0.11 0.15 0.06 0 0 0 0 0.05 0.08
Nucleus 0.39 0.43 0.34 0 0.08 0.05 0.06 – 0.1 0.12 0.11 0.1 0.11 0 0.1 0.14 0.14 0.12 0.19 0.27 0.2 0.13 0.12 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 0.58 0.59 0.95 0.85 0.88 0.84 – 0.8 0.78 0.84 0.86 0.85 0.84 0.84 0.83 0.82 0.81 0.69 0.6 0.65 0.65 0.59 0.62
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.05 0.12 0.11 – 0.12 0.16 0.09 0.12 0.05 0 0 0 0 0 0 0 0 0 0.05 0.08
Vac/Vac Membrane 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 0 – 2 0 0 2 1 0 0 0 0 0 1 2 1 0 1 2
Bud Neck 0 0 0 1 1 0 1 – 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 2 – 4 6 9 8 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 7 1 7 6 14 – 5 2 3 4 5 12 8 5 9 12 0 1 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 3 1 0 0 0 0 0 0
Endosome 3 2 1 0 7 15 15 – 6 10 4 7 1 2 4 0 2 0 1 2 1 0 1 3
Golgi 0 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 53 44 2 23 10 34 26 – 28 25 57 22 22 16 9 30 32 13 3 6 2 1 3 7
Nucleus 179 201 56 4 18 12 18 – 22 27 23 29 10 5 13 37 30 28 97 148 41 5 7 3
Nuclear Periphery 3 0 2 0 1 1 0 – 4 2 2 0 0 0 0 0 0 0 18 9 5 1 1 0
Nucleolus 283 275 96 93 194 212 256 – 173 182 183 243 74 97 112 219 178 190 347 327 135 28 37 57
Peroxisomes 2 1 0 1 5 6 6 – 0 1 1 1 1 3 3 0 3 1 0 0 0 0 0 0
SpindlePole 1 2 1 5 11 29 34 – 26 37 19 35 4 4 4 2 1 0 10 5 2 0 3 7
Vac/Vac Membrane 13 21 11 1 6 4 2 – 1 5 0 2 0 0 3 9 6 7 18 34 15 0 3 7
Unique Cell Count 454 471 163 98 228 242 303 215 233 218 283 87 116 134 264 218 234 501 543 209 43 64 94
Labelled Cell Count 538 552 176 130 260 319 374 274 297 302 354 118 139 158 303 264 252 501 543 209 43 64 94


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.6 34.0 25.0 19.6 19.1 15.4 14.7 – 11.9 11.4 11.7 12.3 23.3 24.8 22.6 49.6 49.0 46.8 – 22.8 23.9
Std Deviation (1e-4) 6.4 10.3 9.1 6.6 5.0 5.4 4.4 – 3.5 3.3 3.6 4.2 10.6 12.4 8.6 21.3 22.4 21.6 – 8.7 11.1
Intensity Change (Log2) – – – -0.35 -0.39 -0.7 -0.76 – -1.07 -1.13 -1.1 -1.03 -0.1 -0.01 -0.14 0.99 0.97 0.91 – -0.13 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.6 -1.0 0.2 – -1.1 0 0 0 0 2.0 0.7 0 -0.1 0.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 2.8 2.5 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.9 1.8 4.4 3.2 – 4.2 3.7 6.7 3.0 6.2 4.2 0 3.9 4.6 2.2
Nucleus -5.6 -6.6 -7.8 -8.0 – -5.7 -5.5 -5.7 -6.2 -3.9 -6.0 -5.0 -4.9 -4.8 -5.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.3 5.8 6.6 6.1 – 4.6 4.1 5.5 6.4 4.2 4.4 4.6 5.5 4.9 4.9
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.4 4.3 4.1 – 4.3 5.1 3.5 4.4 0 0 0 0 0 0
Vacuole 0 -2.0 -2.7 0 – -3.5 -2.3 0 0 0 0 -1.8 -1.6 -1.9 -1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.1368 23.9008 22.332 20.1334 21.8043 20.9351 18.6935 24.1558 20.0672 14.5669 15.9457 20.9912 23.5311 28.5083 24.915 25.103 23.6141 25.3459
Actin 0.0154 0 0.0001 0.0009 0.0046 0.0001 0.0239 0 0.0111 0.0264 0.0365 0.0009 0.0213 0 0.0023 0 0.0061 0
Bud 0.0002 0.0003 0 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0005 0.0001 0.0002 0 0.0001 0 0.0001 0
Bud Neck 0.0004 0.0001 0.0001 0.0001 0.0005 0.0002 0.0002 0 0.0045 0.0049 0.0008 0.0001 0.0017 0 0.0038 0 0.0001 0.0001
Bud Periphery 0.0003 0.0004 0 0.0003 0.0007 0.0003 0.0002 0 0.0003 0.0002 0.001 0.0003 0.0002 0 0.0002 0 0.0002 0.0001
Bud Site 0.0027 0.0002 0.0005 0.0001 0.0038 0.0001 0.0003 0 0.0035 0.0017 0.0029 0.0002 0.0014 0 0.0023 0 0.0003 0
Cell Periphery 0.0005 0.0003 0.0002 0.0008 0.0006 0.0009 0.0008 0.0001 0.0003 0.0006 0.0012 0.0007 0.0003 0.0004 0.0004 0.0001 0.0003 0.0007
Cytoplasm 0.0018 0.0001 0.0003 0.0004 0 0 0.0005 0 0.0027 0.032 0.0004 0.0001 0.0018 0 0 0 0.0004 0
Cytoplasmic Foci 0.0115 0.0004 0.0074 0.0015 0.0004 0.0003 0.0134 0 0.0178 0.0291 0.0166 0.0011 0.0185 0 0.0003 0 0.0112 0.0002
Eisosomes 0.0002 0 0 0 0.0005 0 0.0002 0 0.0002 0.0004 0.0006 0.0001 0.0002 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0016 0 0.0001 0.0011 0.0001 0 0.0003 0 0.0009 0.0017 0.0005 0.0001 0.0011 0 0 0 0.0003 0
Endosome 0.0073 0.0001 0.0007 0.0035 0.0009 0.0002 0.006 0 0.0057 0.0111 0.0052 0.0004 0.006 0 0.0001 0 0.011 0
Golgi 0.0032 0 0.0004 0.001 0.0012 0.0001 0.0036 0 0.0044 0.0058 0.0056 0.0005 0.0026 0 0.0002 0 0.0051 0.0002
Lipid Particles 0.0127 0.0016 0.0031 0.0056 0.0031 0.0078 0.0149 0.0001 0.0134 0.0189 0.0257 0.0094 0.0078 0.0001 0.0016 0.0001 0.0286 0.0123
Mitochondria 0.0058 0.0006 0.0006 0.0022 0.0263 0.0188 0.01 0.0006 0.0067 0.0051 0.0295 0.0247 0.0021 0.0006 0.0007 0.0004 0.0026 0.0107
None 0.0015 0 0.0004 0.0005 0.0002 0.0001 0.0078 0 0.0078 0.0226 0.0009 0.0003 0.0024 0 0 0 0.001 0
Nuclear Periphery 0.0161 0.0224 0.0024 0.0205 0.0128 0.0159 0.0021 0.0014 0.0027 0.0036 0.067 0.0077 0.0082 0.0016 0.0095 0.0001 0.0033 0.0139
Nucleolus 0.7904 0.8221 0.8426 0.8119 0.7507 0.7922 0.7827 0.8582 0.8117 0.706 0.6565 0.8277 0.8181 0.8753 0.8977 0.926 0.6986 0.862
Nucleus 0.0907 0.1453 0.1242 0.1266 0.1778 0.1365 0.1159 0.1383 0.0908 0.1047 0.1184 0.1026 0.0789 0.1171 0.0785 0.0728 0.197 0.0809
Peroxisomes 0.0151 0 0.0053 0.0002 0.001 0.0001 0.0068 0 0.0068 0.0101 0.003 0.0005 0.0037 0 0.0002 0 0.0176 0.0001
Punctate Nuclear 0.0088 0.0006 0.0098 0.0033 0.0012 0.0008 0.0054 0.0003 0.0054 0.0082 0.0014 0.002 0.0207 0.0002 0.0002 0.0002 0.0131 0.0011
Vacuole 0.0021 0.0018 0.0007 0.0032 0.0018 0.0059 0.0015 0.0002 0.0012 0.0032 0.0034 0.0017 0.001 0.0019 0.0002 0.0001 0.0008 0.0012
Vacuole Periphery 0.0118 0.0036 0.0012 0.0163 0.0116 0.0196 0.0034 0.0008 0.0019 0.0034 0.0223 0.0187 0.0016 0.0027 0.0015 0.0002 0.0023 0.0163

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.9751 113.1385 111.9382 80.5007 112.862 92.6418 82.8418 65.609 63.0076 81.4388
Translational Efficiency 1.3295 1.3402 1.0148 1.5227 0.9838 1.0758 1.2759 1.1898 1.0724 1.1445

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer
Localization
Cell Percentages nucleolus (50%), nucleus (9%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nop2

Nop2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available