Standard name
Description rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.1 0.06 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.09 0 0.23 0 0.14 0.09 0.13 0.11 0.26 0.08 0.25 0.14 0.07 0.11 0.15 0.06 0 0 0 0 0.05 0.08
Nucleus 0.39 0.43 0.34 0 0.08 0.05 0.06 0.1 0.12 0.11 0.1 0.11 0 0.1 0.14 0.14 0.12 0.19 0.27 0.2 0.13 0.12 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 0.58 0.59 0.95 0.85 0.88 0.84 0.8 0.78 0.84 0.86 0.85 0.84 0.84 0.83 0.82 0.81 0.69 0.6 0.65 0.65 0.59 0.62
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.05 0.12 0.11 0.12 0.16 0.09 0.12 0.05 0 0 0 0 0 0 0 0 0 0.05 0.08
Vac/Vac Membrane 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 0 2 0 0 2 1 0 0 0 0 0 1 2 1 0 1 2
Bud Neck 0 0 0 1 1 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 2 4 6 9 8 0 0 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 7 1 7 6 14 5 2 3 4 5 12 8 5 9 12 0 1 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 3 1 0 0 0 0 0 0
Endosome 3 2 1 0 7 15 15 6 10 4 7 1 2 4 0 2 0 1 2 1 0 1 3
Golgi 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 53 44 2 23 10 34 26 28 25 57 22 22 16 9 30 32 13 3 6 2 1 3 7
Nucleus 179 201 56 4 18 12 18 22 27 23 29 10 5 13 37 30 28 97 148 41 5 7 3
Nuclear Periphery 3 0 2 0 1 1 0 4 2 2 0 0 0 0 0 0 0 18 9 5 1 1 0
Nucleolus 283 275 96 93 194 212 256 173 182 183 243 74 97 112 219 178 190 347 327 135 28 37 57
Peroxisomes 2 1 0 1 5 6 6 0 1 1 1 1 3 3 0 3 1 0 0 0 0 0 0
SpindlePole 1 2 1 5 11 29 34 26 37 19 35 4 4 4 2 1 0 10 5 2 0 3 7
Vac/Vac Membrane 13 21 11 1 6 4 2 1 5 0 2 0 0 3 9 6 7 18 34 15 0 3 7
Unique Cell Count 454 471 163 98 228 242 303 215 233 218 283 87 116 134 264 218 234 501 543 209 43 64 94
Labelled Cell Count 538 552 176 130 260 319 374 274 297 302 354 118 139 158 303 264 252 501 543 209 43 64 94


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.6 34.0 25.0 19.6 19.1 15.4 14.7 11.9 11.4 11.7 12.3 23.3 24.8 22.6 49.6 49.0 46.8 22.8 23.9
Std Deviation (1e-4) 6.4 10.3 9.1 6.6 5.0 5.4 4.4 3.5 3.3 3.6 4.2 10.6 12.4 8.6 21.3 22.4 21.6 8.7 11.1
Intensity Change (Log2) -0.35 -0.39 -0.7 -0.76 -1.07 -1.13 -1.1 -1.03 -0.1 -0.01 -0.14 0.99 0.97 0.91 -0.13 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.6 -1.0 0.2 -1.1 0 0 0 0 2.0 0.7 0 -0.1 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 2.8 2.5 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.9 1.8 4.4 3.2 4.2 3.7 6.7 3.0 6.2 4.2 0 3.9 4.6 2.2
Nucleus -5.6 -6.6 -7.8 -8.0 -5.7 -5.5 -5.7 -6.2 -3.9 -6.0 -5.0 -4.9 -4.8 -5.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.3 5.8 6.6 6.1 4.6 4.1 5.5 6.4 4.2 4.4 4.6 5.5 4.9 4.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.4 4.3 4.1 4.3 5.1 3.5 4.4 0 0 0 0 0 0
Vacuole 0 -2.0 -2.7 0 -3.5 -2.3 0 0 0 0 -1.8 -1.6 -1.9 -1.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available