Standard name
Description Dual-specificity protein phosphatase; maintains low levels of both basal and induced cell integrity pathway signaling by dephosphorylation of the Slt2p MAPK; reciprocally regulated by Slt2p through phosphorylation; minor role with Ptp2p in the adaptive response to pheromone through the dephosphorylation of the Fus3p MAPK with major contribution by Ptp3p; inhibits the nuclear accumulation of Fus3p; two isoforms exist as the result of alternative translation initiation starts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.95 0.93 0.98 0.95 0.86 0.86 0.69 0.77 0.57 0.66 0.99 1.0 0.99 0.96 0.94 0.88 0.93 0.91 0.84 0.95 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.12 0 0.23 0.26 0.32 0.5 0.51 0.57 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0
Bud 1 0 0 2 2 0 0 3 0 3 1 0 2 0 1 1 0 0 4 0 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 2 1 1 2 0 1 3 1 2 1 0 0 3 2 4 0 0 0 0 0 0
Cytoplasm 274 122 109 100 87 155 197 178 101 171 83 100 225 223 269 129 113 46 277 115 109 115 169 127
Endoplasmic Reticulum 0 1 1 1 0 0 0 0 0 0 3 1 2 0 0 10 7 2 0 0 1 0 3 5
Endosome 0 0 0 1 1 0 1 0 0 0 1 0 0 0 1 1 3 0 3 0 2 1 2 0
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0 2 0 0 0 1 1
Mitochondria 16 1 3 13 4 38 59 66 73 113 83 81 0 3 0 5 0 1 2 0 1 0 1 0
Nucleus 2 0 0 3 0 3 1 1 0 0 1 1 0 0 0 0 0 3 0 0 0 0 0 0
Nuclear Periphery 2 1 2 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0
Nucleolus 1 0 1 0 0 0 1 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 3 1 1 1 0 0 7 3 1 6 1 3 0 0 0 2 6 2 1 6 3 0 0 0
Unique Cell Count 287 124 115 108 89 164 228 208 147 223 145 151 228 224 271 135 120 52 299 127 131 121 188 138
Labelled Cell Count 300 127 118 125 95 197 269 251 176 297 174 189 231 227 272 154 132 58 299 127 131 121 188 138


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.8 5.4 4.9 5.3 4.6 4.2 4.1 4.5 3.9 4.1 3.8 5.8 5.4 5.6 7.1 9.1 8.8 6.8 7.4 8.7
Std Deviation (1e-4) 0.6 1.2 1.4 1.3 1.7 1.4 1.6 1.0 1.9 1.5 1.4 1.5 0.9 1.1 1.1 1.9 3.3 2.5 1.5 1.6 1.8
Intensity Change (Log2) – – – -0.13 -0.03 -0.23 -0.37 -0.39 -0.27 -0.48 -0.41 -0.51 0.09 0.01 0.05 0.4 0.75 0.71 0.33 0.44 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 1.1 -0.1 -2.4 -2.5 -5.3 -4.2 -6.8 -5.6 2.1 2.9 2.8 0.3 -0.2 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0