Standard name
Human Ortholog
Description Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SFB2 has a paralog, SEC24, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.31 0.25 0.43 0.25 0.2 0.1 0 0.13 0.12 0.05 0.13 0.1 0.15 0.13 0.1 0.32 0.18 0.14 0.24 0.22 0.43 0.26 0.2 0.16
Bud 0 0 0 0.05 0 0 0 0 0 0 0.05 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.08 0.08 0.09 0 0 0 0.05 0 0.06 0 0.09 0.06 0.08 0.11 0.07 0.2 0 0 0 0.05 0.06 0
Cytoplasm 0.31 0.3 0.2 0.17 0.09 0.11 0.14 0.1 0.11 0.08 0.08 0.05 0.35 0.56 0.58 0.57 0.66 0.61 0.06 0.06 0 0 0.06 0.07
Endoplasmic Reticulum 0.52 0.62 0.58 0.4 0.62 0.48 0.29 0.33 0.38 0.24 0.26 0.3 0.55 0.41 0.39 0.06 0.08 0.05 0.48 0.55 0.31 0.45 0.47 0.55
Endosome 0 0 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.07 0.1 0.07 0.07 0.08
Mitochondria 0 0 0 0.32 0.21 0.64 0.69 0.64 0.73 0.81 0.77 0.77 0 0 0 0.08 0.08 0.05 0 0 0.05 0.07 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 137 89 177 41 52 40 17 56 50 28 52 40 55 26 22 88 24 12 134 87 218 64 58 49
Bud 7 8 12 8 6 14 17 16 13 20 20 22 10 2 2 17 5 2 1 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 4 2 3 2 5
Bud Site 0 0 0 0 0 0 0 3 0 1 1 0 0 0 0 0 0 0
Cell Periphery 19 16 33 13 23 6 14 11 20 14 24 14 34 12 17 29 9 17 11 8 16 11 16 8
Cytoplasm 136 107 80 27 23 45 65 43 46 44 33 19 131 109 131 156 88 53 31 21 9 11 18 20
Endoplasmic Reticulum 231 217 236 66 164 199 135 148 163 123 104 118 206 79 88 17 10 4 266 213 157 113 138 168
Endosome 13 1 22 11 14 6 11 15 2 5 3 4 0 0 0 3 3 3 7 5 16 2 3 5
Golgi 20 2 11 3 13 4 4 4 8 3 3 8 0 1 1 3 0 2 64 27 48 18 21 23
Mitochondria 13 8 6 52 55 268 316 288 313 418 309 307 0 0 0 21 11 4 17 9 22 17 20 13
Nucleus 0 1 0 0 0 1 1 2 3 0 1 0 1 0 3 2 5 2 2 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2 0 3 5 9 6 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 2 11 6 0 5 1 0 4 3 3 0 0 0 0 0 0 1 0 3 1 0 0
SpindlePole 0 1 2 0 1 0 0 4 0 2 1 0 2 0 0 3 2 2 0 0 0 0 0 0
Vac/Vac Membrane 3 0 4 0 1 2 6 9 2 4 1 3 0 0 0 0 0 2 6 7 0 5 5 4
Unique Cell Count 443 352 407 163 264 416 458 447 429 519 402 397 375 194 226 276 133 87 556 391 506 253 294 306
Labelled Cell Count 581 450 585 232 358 587 591 604 625 675 562 540 439 229 264 339 158 103 556 391 506 253 294 306


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 9.1 7.6 6.6 6.6 5.4 5.5 5.4 5.6 5.0 5.3 5.3 9.0 9.4 9.7 7.4 8.5 9.4 9.0 9.6 10.4
Std Deviation (1e-4) 0.9 1.2 1.0 0.9 1.0 1.0 1.1 1.3 0.9 1.0 1.2 1.0 0.9 1.1 1.1 1.0 1.4 1.7 1.2 1.5 1.5
Intensity Change (Log2) -0.19 -0.2 -0.47 -0.47 -0.47 -0.44 -0.59 -0.51 -0.51 0.24 0.31 0.36 -0.04 0.18 0.32 0.25 0.34 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.1 -6.3 -11.0 -14.0 -10.1 -10.3 -13.9 -9.6 -10.7 -8.8 -7.3 -8.8 -3.1 -5.3 -5.2
Bud 1.1 -0.5 0.3 0.6 0.5 0.1 0.7 1.5 1.8 -0.2 0 0 2.0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 0.3 -4.5 -3.3 -3.7 -2.0 -3.7 -1.2 -2.8 0.5 -0.8 -0.3 1.1 -0.5 3.2
Cytoplasm -0.9 -3.8 -3.5 -2.1 -4.2 -3.6 -5.0 -4.7 -6.4 4.8 9.0 9.8 9.9 10.1 7.9
Endoplasmic Reticulum -3.8 1.1 -2.9 -8.5 -7.3 -5.8 -10.6 -9.3 -8.1 -0.9 -4.0 -4.6 -13.8 -10.1 -9.0
Endosome 0.6 -0.1 -3.1 -2.3 -1.5 -4.3 -4.0 -3.8 -3.5 -4.6 -3.3 -3.6 -2.9 -1.5 0
Golgi 0 1.5 -1.9 -2.1 -2.0 -0.8 -2.6 -2.1 -0.6 -3.2 0 0 -1.5 0 0
Mitochondria 10.9 8.5 19.2 20.5 19.3 21.3 24.0 22.0 22.1 0 0 0 4.0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.2 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2002 6.0742 5.5051 5.1601 5.2337 5.5666 8.1394 8.3793 8.3267 8.2394 8.4924 8.1902 7.8687 8.6017 8.5624 7.9378 7.6534 8.4169
Actin 0.1481 0.1165 0.1445 0.1468 0.0793 0.0983 0.228 0.1371 0.1433 0.1824 0.1599 0.1089 0.1512 0.1333 0.1649 0.1185 0.2779 0.1491
Bud 0.0017 0.0071 0.0012 0.0013 0.0004 0.0008 0.0009 0.0013 0.0025 0.0015 0.0007 0.0006 0.0003 0.0005 0.0005 0.0009 0.0003 0.0003
Bud Neck 0.0032 0.0019 0.0037 0.0037 0.0015 0.0139 0.0019 0.0019 0.0024 0.0071 0.002 0.009 0.0018 0.0023 0.0018 0.0047 0.0079 0.0065
Bud Periphery 0.0027 0.0027 0.0013 0.0039 0.0003 0.0013 0.0009 0.0013 0.0027 0.0019 0.0009 0.0012 0.0004 0.0004 0.0007 0.0018 0.0003 0.0004
Bud Site 0.0129 0.0157 0.0066 0.0012 0.0007 0.0032 0.0026 0.0173 0.0099 0.002 0.0005 0.001 0.0012 0.0138 0.0013 0.0031 0.0012 0.0006
Cell Periphery 0.0021 0.0048 0.0021 0.0037 0.0004 0.0027 0.0024 0.0043 0.0046 0.0039 0.0007 0.0021 0.0087 0.0023 0.0043 0.0063 0.0017 0.0023
Cytoplasm 0.1116 0.2809 0.1564 0.1158 0.1355 0.2283 0.1508 0.2594 0.1547 0.2028 0.1368 0.1847 0.1565 0.1772 0.1009 0.2044 0.171 0.1417
Cytoplasmic Foci 0.0776 0.0855 0.0756 0.0621 0.0934 0.0856 0.0863 0.0816 0.0909 0.0568 0.0645 0.0857 0.0738 0.0832 0.0706 0.0484 0.0571 0.0666
Eisosomes 0.0014 0.0008 0.0007 0.0007 0.0001 0.0007 0.001 0.0018 0.0014 0.0008 0.0006 0.0004 0.0025 0.0005 0.0016 0.001 0.0008 0.0013
Endoplasmic Reticulum 0.0895 0.0818 0.079 0.1814 0.0683 0.1138 0.0426 0.0554 0.0365 0.0924 0.145 0.0969 0.0519 0.1336 0.0785 0.1319 0.16 0.134
Endosome 0.1419 0.1189 0.1382 0.1501 0.1646 0.1356 0.129 0.098 0.1336 0.1196 0.106 0.1266 0.0663 0.1053 0.107 0.0845 0.0353 0.0841
Golgi 0.2972 0.2488 0.3459 0.2785 0.4121 0.2786 0.2788 0.2731 0.3633 0.2702 0.2669 0.3265 0.4098 0.3046 0.4352 0.3592 0.2621 0.3857
Lipid Particles 0.0312 0.0126 0.0143 0.0215 0.0224 0.0134 0.0175 0.0146 0.0239 0.0078 0.0141 0.0261 0.0392 0.0268 0.0236 0.0132 0.0166 0.0156
Mitochondria 0.0209 0.0109 0.0134 0.0192 0.0067 0.0073 0.0072 0.0078 0.009 0.0047 0.0122 0.0089 0.0026 0.003 0.0033 0.0069 0.0014 0.0045
None 0.0087 0.0052 0.0029 0.0008 0.0008 0.0024 0.0072 0.0366 0.0095 0.0403 0.003 0.0144 0.0136 0.0018 0.0017 0.0072 0.0008 0.0026
Nuclear Periphery 0.0054 0.0014 0.0022 0.0035 0.0012 0.0041 0.0134 0.0017 0.0016 0.0012 0.0647 0.0009 0.0009 0.0053 0.001 0.0018 0.0009 0.0012
Nucleolus 0.001 0.0001 0.0001 0 0.0001 0.0005 0.0005 0.0002 0.0001 0 0.0003 0.0001 0.0002 0.0001 0 0.0001 0 0
Nucleus 0.0188 0.0004 0.0005 0.0004 0.0003 0.0012 0.0158 0.0014 0.0005 0.0003 0.0059 0.0003 0.0003 0.0006 0.0001 0.0004 0.0001 0.0002
Peroxisomes 0.0095 0.0014 0.006 0.0013 0.0075 0.0034 0.006 0.0016 0.0057 0.0009 0.0069 0.002 0.0074 0.0017 0.0011 0.0014 0.0035 0.0017
Punctate Nuclear 0.0087 0.0005 0.0008 0.0005 0.0019 0.001 0.0029 0.0007 0.0007 0.0003 0.0044 0.0004 0.0099 0.0008 0.0003 0.0004 0.0003 0.0003
Vacuole 0.0041 0.0017 0.0029 0.0021 0.0015 0.0027 0.0023 0.0024 0.0021 0.0022 0.0014 0.0024 0.001 0.0018 0.0008 0.0026 0.0006 0.0008
Vacuole Periphery 0.0018 0.0006 0.0014 0.0015 0.001 0.0013 0.0017 0.0008 0.0011 0.0009 0.0025 0.0009 0.0005 0.0011 0.0007 0.0013 0.0003 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.9376 24.7438 29.4888 28.0325 29.6345 31.8557 32.884 36.202 28.4459 34.1362
Translational Efficiency 0.6851 0.9636 0.7054 0.6062 0.6022 0.6084 0.6835 0.5308 0.6336 0.5663

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
283 1847 1414 1141 2006 1665 861 1540 2289 3512 2275 2681

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1032.96 1024.10 1100.42 1130.64 898.50 987.79 1064.78 1101.48 915.12 1006.89 1086.93 1113.89
Standard Deviation 171.45 171.36 162.47 147.16 126.50 150.90 120.15 155.02 140.06 163.00 148.89 152.41
Intensity Change Log 2 -0.012428 0.091270 0.130355 0.136686 0.244965 0.293853 0.058860 0.164808 0.208723

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.038470 0.041130 0.044198 0.103266* 0.029758 0.041258 0.047852 0.061861 0.030835 0.041191 0.045581 0.079483
Bud Neck 0.008496 0.000365 0.001631 0.005885 0.000710 0.000535 0.002399 0.004764 0.001673 0.000446 0.001922 0.005241
Bud Site 0.060960 0.003551 0.014864 0.054454 0.002968 0.002480 0.018465 0.033073 0.010138 0.003043 0.016227 0.042173
Cell Periphery 0.003914 0.005921 0.006654 0.015176 0.008433 0.009850 0.007261 0.011769 0.007874 0.007784 0.006884 0.013219
Cytoplasm 0.074315 0.023644 0.040430 0.086664 0.035262 0.026723 0.049133 0.084418 0.040091 0.025104 0.043724 0.085374
Cytoplasmic Foci 0.208010* 0.124786 0.091764 0.139327 0.087597 0.087790 0.122502 0.170873 0.102484 0.107247 0.103397 0.157447
Eisosomes 0.003625 0.010804 0.004246 0.009737 0.018420 0.016341 0.007488 0.008457 0.016591 0.013429 0.005473 0.009001
Endoplasmic Reticulum 0.069768 0.105946* 0.318427* 0.094656 0.070812 0.075955 0.190448* 0.071461 0.070683 0.091728 0.269992* 0.081333
Endosome 0.143517 0.051448 0.070350 0.065893 0.031878 0.044913 0.070355 0.092452 0.045681 0.048350 0.070352 0.081149
Golgi 0.077191 0.273186* 0.108897 0.096416 0.260823* 0.271747* 0.126412* 0.144192* 0.238119* 0.272504* 0.115526* 0.123859*
Lipid Particles 0.109062* 0.257765* 0.263766* 0.242007* 0.229712* 0.262649* 0.292724* 0.231895* 0.214796* 0.260080* 0.274726* 0.236199*
Mitochondria 0.004470 0.001640 0.001115 0.003936 0.005532 0.004072 0.001680 0.003333 0.005400 0.002793 0.001329 0.003590
Mitotic Spindle 0.018454 0.000119 0.001624 0.009110 0.000060 0.000093 0.004785 0.005950 0.002334 0.000106 0.002820 0.007295
None 0.000031 0.000965 0.001035 0.000173 0.001179 0.000915 0.000436 0.000358 0.001037 0.000941 0.000809 0.000280
Nuclear Periphery 0.000927 0.000022 0.000248 0.000116 0.000023 0.000031 0.000077 0.000220 0.000135 0.000026 0.000183 0.000176
Nuclear Periphery Foci 0.007091 0.000386 0.008434 0.003001 0.000275 0.000311 0.002011 0.003718 0.001118 0.000350 0.006003 0.003413
Nucleolus 0.000107 0.000010 0.000037 0.000046 0.000007 0.000009 0.000021 0.000208 0.000019 0.000009 0.000031 0.000139
Nucleus 0.002083 0.000138 0.000435 0.001084 0.000211 0.000175 0.000618 0.000985 0.000443 0.000155 0.000504 0.001027
Peroxisomes 0.013844 0.096482 0.012023 0.031763 0.214663* 0.151669* 0.042663 0.032754 0.189835* 0.122646* 0.023619 0.032332
Vacuole 0.145058 0.000752 0.008055 0.033165 0.000522 0.000986 0.010524 0.032234 0.018391 0.000863 0.008990 0.032630
Vacuole Periphery 0.010605 0.000940 0.001766 0.004126 0.001156 0.001500 0.002145 0.005025 0.002324 0.001206 0.001909 0.004643

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.44 -0.87 -7.72 -10.55 -9.49 -3.78 -4.18 -8.44 -4.85 -2.66 -4.40 -5.09 -13.90 -10.99 -8.73
Bud Neck 4.17 3.48 1.24 -11.31 -7.82 1.41 -4.49 -11.72 -12.49 -4.59 4.63 -0.88 -9.42 -16.89 -9.43
Bud Site 7.37 5.87 0.83 -15.11 -11.04 1.09 -6.72 -13.29 -13.64 -4.51 6.36 -3.74 -14.40 -20.17 -11.45
Cell Periphery -1.66 -2.05 -6.24 -6.05 -5.24 -1.04 1.03 -2.00 -1.03 -2.78 0.09 1.06 -4.19 -4.61 -5.45
Cytoplasm 7.13 4.64 -1.39 -15.05 -9.94 4.79 -4.13 -13.27 -15.71 -7.42 8.99 -1.55 -15.00 -21.88 -12.87
Cytoplasmic Foci 7.30 10.25 5.75 -2.32 -7.81 -0.05 -5.82 -16.43 -16.07 -7.04 -1.33 -0.25 -12.65 -12.52 -12.27
Eisosomes -5.27 -0.51 -3.96 1.29 -5.94 2.02 11.71 11.09 8.25 -1.05 4.09 15.36 9.66 6.96 -5.77
Endoplasmic Reticulum -3.90 -21.12 -2.53 1.84 23.69 -1.13 -12.89 -0.05 1.00 12.59 -5.40 -29.59 -2.49 2.71 27.80
Endosome 10.28 7.89 8.33 -4.45 1.15 -7.20 -10.85 -18.60 -13.93 -4.82 -1.48 -9.45 -13.25 -13.58 -3.56
Golgi -23.74* -4.28 -2.50 30.47* 2.62 -1.68 20.12 19.46 21.17 -2.69 -6.47 24.05 22.28 35.17 -1.90
Lipid Particles -13.50 -13.10 -10.72 1.78 2.20 -4.68 -6.07 -0.17 3.85 5.49 -8.04 -8.66 -3.23 4.04 5.36
Mitochondria 1.60 1.92 0.24 -2.31 -2.87 1.66 4.91 2.94 1.15 -2.98 3.78 6.24 2.38 -1.41 -4.51
Mitotic Spindle 3.62 3.29 1.66 -4.63 -3.71 -1.35 -2.71 -5.15 -5.13 -0.53 3.49 -0.61 -4.16 -6.82 -3.43
None -3.63 -3.07 -3.90 3.04 2.62 0.65 2.20 2.75 1.66 0.32 0.31 0.71 3.06 3.09 2.29
Nuclear Periphery 2.28 1.69 2.03 -6.37 2.51 -0.98 -4.62 -5.76 -5.39 -3.92 2.18 -0.89 -0.88 -7.14 0.13
Nuclear Periphery Foci 6.41 -0.95 3.61 -7.86 4.97 -0.62 -6.40 -5.98 -5.93 -2.82 5.26 -7.43 -6.07 -8.52 3.32
Nucleolus 2.61 1.64 1.49 -3.30 -0.52 -0.87 -2.27 -1.26 -1.25 -1.18 1.97 -0.83 -1.31 -1.41 -1.19
Nucleus 4.41 3.73 2.07 -6.83 -4.59 2.37 -3.45 -3.86 -4.02 -1.53 5.01 -1.03 -4.27 -6.59 -3.72
Peroxisomes -20.53* 0.98 -6.73 15.28 -8.45 8.69 27.08* 31.88* 22.70 2.55 11.61 32.49 30.86 26.83 -4.15
Vacuole 11.50 10.91 8.68 -13.08 -9.86 -2.99 -7.24 -15.19 -14.95 -8.68 9.51 4.81 -5.98 -19.88 -13.71
Vacuole Periphery 4.94 4.49 3.12 -5.51 -3.96 -1.51 -1.71 -6.59 -5.79 -3.56 3.86 1.09 -4.80 -8.13 -5.76