Standard name
Description Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SFB2 has a paralog, SEC24, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.31 0.25 0.43 0.25 0.2 0.1 0 0.13 0.12 0.05 0.13 0.1 0.15 0.13 0.1 0.32 0.18 0.14 0.24 0.22 0.43 0.26 0.2 0.16
Bud 0 0 0 0.05 0 0 0 0 0 0 0.05 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.08 0.08 0.09 0 0 0 0.05 0 0.06 0 0.09 0.06 0.08 0.11 0.07 0.2 0 0 0 0.05 0.06 0
Cytoplasm 0.31 0.3 0.2 0.17 0.09 0.11 0.14 0.1 0.11 0.08 0.08 0.05 0.35 0.56 0.58 0.57 0.66 0.61 0.06 0.06 0 0 0.06 0.07
Endoplasmic Reticulum 0.52 0.62 0.58 0.4 0.62 0.48 0.29 0.33 0.38 0.24 0.26 0.3 0.55 0.41 0.39 0.06 0.08 0.05 0.48 0.55 0.31 0.45 0.47 0.55
Endosome 0 0 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.07 0.1 0.07 0.07 0.08
Mitochondria 0 0 0 0.32 0.21 0.64 0.69 0.64 0.73 0.81 0.77 0.77 0 0 0 0.08 0.08 0.05 0 0 0.05 0.07 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 137 89 177 41 52 40 17 56 50 28 52 40 55 26 22 88 24 12 134 87 218 64 58 49
Bud 7 8 12 8 6 14 17 16 13 20 20 22 10 2 2 17 5 2 1 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 4 2 3 2 5
Bud Site 0 0 0 0 0 0 0 3 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 19 16 33 13 23 6 14 11 20 14 24 14 34 12 17 29 9 17 11 8 16 11 16 8
Cytoplasm 136 107 80 27 23 45 65 43 46 44 33 19 131 109 131 156 88 53 31 21 9 11 18 20
Endoplasmic Reticulum 231 217 236 66 164 199 135 148 163 123 104 118 206 79 88 17 10 4 266 213 157 113 138 168
Endosome 13 1 22 11 14 6 11 15 2 5 3 4 0 0 0 3 3 3 7 5 16 2 3 5
Golgi 20 2 11 3 13 4 4 4 8 3 3 8 0 1 1 3 0 2 64 27 48 18 21 23
Mitochondria 13 8 6 52 55 268 316 288 313 418 309 307 0 0 0 21 11 4 17 9 22 17 20 13
Nucleus 0 1 0 0 0 1 1 2 3 0 1 0 1 0 3 2 5 2 2 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2 0 3 5 9 6 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 2 11 6 0 5 1 0 4 3 3 0 0 0 0 0 0 1 0 3 1 0 0
SpindlePole 0 1 2 0 1 0 0 4 0 2 1 0 2 0 0 3 2 2 0 0 0 0 0 0
Vac/Vac Membrane 3 0 4 0 1 2 6 9 2 4 1 3 0 0 0 0 0 2 6 7 0 5 5 4
Unique Cell Count 443 352 407 163 264 416 458 447 429 519 402 397 375 194 226 276 133 87 556 391 506 253 294 306
Labelled Cell Count 581 450 585 232 358 587 591 604 625 675 562 540 439 229 264 339 158 103 556 391 506 253 294 306


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 9.1 7.6 6.6 6.6 5.4 5.5 5.4 5.6 5.0 5.3 5.3 9.0 9.4 9.7 7.4 8.5 9.4 9.0 9.6 10.4
Std Deviation (1e-4) 0.9 1.2 1.0 0.9 1.0 1.0 1.1 1.3 0.9 1.0 1.2 1.0 0.9 1.1 1.1 1.0 1.4 1.7 1.2 1.5 1.5
Intensity Change (Log2) – – – -0.19 -0.2 -0.47 -0.47 -0.47 -0.44 -0.59 -0.51 -0.51 0.24 0.31 0.36 -0.04 0.18 0.32 0.25 0.34 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.1 -6.3 -11.0 -14.0 -10.1 -10.3 -13.9 -9.6 -10.7 -8.8 -7.3 -8.8 -3.1 -5.3 -5.2
Bud 1.1 -0.5 0.3 0.6 0.5 0.1 0.7 1.5 1.8 -0.2 0 0 2.0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 0.3 -4.5 -3.3 -3.7 -2.0 -3.7 -1.2 -2.8 0.5 -0.8 -0.3 1.1 -0.5 3.2
Cytoplasm -0.9 -3.8 -3.5 -2.1 -4.2 -3.6 -5.0 -4.7 -6.4 4.8 9.0 9.8 9.9 10.1 7.9
Endoplasmic Reticulum -3.8 1.1 -2.9 -8.5 -7.3 -5.8 -10.6 -9.3 -8.1 -0.9 -4.0 -4.6 -13.8 -10.1 -9.0
Endosome 0.6 -0.1 -3.1 -2.3 -1.5 -4.3 -4.0 -3.8 -3.5 -4.6 -3.3 -3.6 -2.9 -1.5 0
Golgi 0 1.5 -1.9 -2.1 -2.0 -0.8 -2.6 -2.1 -0.6 -3.2 0 0 -1.5 0 0
Mitochondria 10.9 8.5 19.2 20.5 19.3 21.3 24.0 22.0 22.1 0 0 0 4.0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.2 0 0 0 0 0 0 0 0 0 0