Standard name
Description Leucyl aminopeptidase yscIV with epoxide hydrolase activity; metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; also known as leukotriene A4 hydrolase
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.95 0.92 0.92 0.87 – 0.8 0.73 0.71 0.71 1.0 0.99 0.96 – 0.95 0.93 0.92 0.92 0.85 0.8 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.11 0.06 0.29 0.19 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 – 0 0 0 0 0 0.08 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.05 0.06 – 0.05 0.16 0.05 0.13 0 0 0 – 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 4 0 1 0 0 0 – 0 0 0 2 0 0 0 1
Bud 0 0 0 0 0 1 1 – 0 1 0 1 0 0 0 – 0 0 4 3 2 0 1 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 4 2 0 0 3 4 – 9 11 2 7 0 1 1 – 6 3 0 0 0 0 0 0
Cytoplasm 139 232 89 82 173 226 234 – 172 205 141 201 60 112 134 – 88 43 130 216 100 19 83 144
Endoplasmic Reticulum 0 5 1 0 2 2 7 – 4 6 4 3 0 0 3 – 2 0 0 2 3 0 0 0
Endosome 0 0 0 1 0 0 1 – 0 0 0 0 0 1 0 – 0 0 1 2 0 0 0 1
Golgi 0 0 0 0 1 1 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 2 6 11 – 24 17 58 53 0 0 1 – 0 0 1 0 3 0 0 0
Nucleus 1 0 2 3 1 3 9 – 4 7 10 15 2 4 1 – 0 0 0 0 0 1 0 2
Nuclear Periphery 0 0 0 0 0 0 0 – 1 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0
Vac/Vac Membrane 1 1 1 1 11 13 15 – 10 44 10 36 0 0 1 – 4 3 0 4 4 0 1 0
Unique Cell Count 141 235 92 86 188 245 270 216 280 200 284 60 113 139 93 46 142 236 119 25 92 160
Labelled Cell Count 142 242 95 87 190 256 282 226 296 226 317 62 118 141 100 49 142 236 119 25 92 160


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 13.4 10.8 10.1 11.2 9.3 9.3 – 8.4 8.5 7.4 7.4 11.6 12.4 11.7 – 21.7 19.7 – 10.7 12.3
Std Deviation (1e-4) 2.9 3.8 3.0 2.2 3.0 2.3 2.4 – 2.4 2.2 2.0 2.0 3.0 2.9 2.9 – 5.7 5.2 – 2.7 3.6
Intensity Change (Log2) – – – -0.1 0.05 -0.22 -0.23 – -0.36 -0.34 -0.55 -0.54 0.1 0.2 0.12 – 1.01 0.86 – -0.01 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cytoplasm -0.5 -1.5 -1.5 -2.7 – -3.8 -4.8 -5.1 -5.2 1.4 1.2 -0.1 – -0.7 -0.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 – 3.3 0 5.8 4.5 0 0 0 – 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0 0 0 – 0 3.7 0 3.2 0 0 0 – 0 0