Standard name
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.09 0 – 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.1 0 0 0 0.05 0.05 0.06
Bud 0 0 0.07 – 0 0 0 0 0 0.06 0.07 0.07 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.05 – 0.08 0.05 0.06 0.15 0.05 0.08 0.09 0.09 0.05 0.06 0.05 0.08 0.08 0.1 0 0 0.05 0 0 0
Endoplasmic Reticulum 0.07 0.11 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0.09 0 0.06 0 0 0 0 0 0
Endosome 0.07 0 0.21 – 0.28 0.15 0.08 0.15 0 0 0 0 0 0.11 0.07 0.12 0.22 0.18 0.11 0.12 0.14 0.1 0.08 0.07
Golgi 0.44 0.28 0.17 – 0 0 0 0 0 0 0 0 0.26 0.41 0.21 0.47 0.52 0.51 0.74 0.59 0.55 0.63 0.69 0.72
Mitochondria 0.75 0.69 0.56 – 0.67 0.8 0.85 0.66 0.91 0.9 0.87 0.88 0.81 0.52 0.74 0.12 0.12 0.1 0.08 0.16 0.16 0.14 0.1 0.06
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0
SpindlePole 0 0 0.05 – 0.06 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.07 0.19 – 0.19 0.08 0 0.08 0.05 0.08 0 0 0 0.1 0.05 0.11 0.16 0.13 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 32 3 – 1 0 0 1 0 2 0 0 5 3 4 16 16 8 2 12 1 12 21 22
Bud 2 8 6 – 0 4 5 3 4 9 7 7 6 3 8 7 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 2 2 2 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 1 – 0 2 0 1 0 0 0 0 0 2 1 2 0 2 0 0 0 0 0 1
Cytoplasm 4 33 4 – 3 5 9 24 8 13 9 9 17 26 21 10 10 8 0 5 5 0 0 3
Endoplasmic Reticulum 11 41 0 – 0 0 1 0 5 0 0 0 17 20 23 12 4 5 0 3 0 1 3 5
Endosome 12 13 17 – 10 16 12 23 3 0 0 0 12 50 26 16 27 15 20 52 15 25 35 28
Golgi 74 102 14 – 1 0 1 5 1 1 0 0 92 179 82 63 64 42 135 264 62 168 312 282
Mitochondria 126 253 45 – 24 86 123 102 140 138 88 88 283 229 286 16 15 8 13 70 18 37 43 24
Nucleus 1 0 1 – 0 0 3 4 0 5 3 3 0 2 2 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 – 0 0 0 0 0 0 2 2 1 0 2 3 1 2 0 2 1 0 0 0
Nucleolus 1 0 1 – 0 4 0 0 0 0 1 1 1 0 2 1 2 0 0 0 0 0 0 0
Peroxisomes 0 1 1 – 1 6 4 9 4 0 1 1 1 3 1 0 0 0 3 5 3 14 27 14
SpindlePole 0 2 4 – 2 0 4 10 1 5 2 2 2 2 2 5 5 2 0 2 0 1 1 0
Vac/Vac Membrane 12 25 15 – 7 9 6 12 7 12 3 2 15 42 21 15 20 11 2 22 1 2 3 1
Unique Cell Count 168 368 80 36 108 144 155 154 153 101 100 351 441 384 133 123 83 183 446 114 266 454 391
Labelled Cell Count 263 515 112 49 132 168 196 176 187 117 117 452 561 481 167 165 104 183 446 114 266 454 391


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 7.2 7.5 – 7.4 5.9 5.8 6.7 5.2 4.8 4.6 4.6 7.4 7.7 7.2 9.2 9.5 9.0 6.8 7.1 7.6
Std Deviation (1e-4) 1.0 1.2 1.4 – 1.9 1.3 1.4 2.1 1.3 1.3 1.2 1.2 1.0 1.2 1.2 1.8 1.9 1.9 1.1 1.1 1.3
Intensity Change (Log2) – – – – -0.02 -0.36 -0.37 -0.16 -0.54 -0.64 -0.72 -0.72 -0.03 0.03 -0.06 0.29 0.33 0.26 -0.14 -0.09 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 2.1 2.2 1.5
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 2.4 0 1.0 1.0 1.0 0 0 0 0.7 0.9 1.1
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0.8 -1.1 -2.8 -1.2 -5.0 0 0 0 -5.7 -2.4 -4.1 -1.8 0.1 -0.5
Golgi – 0 -4.5 -4.8 -3.8 -5.0 0 0 0 1.6 3.9 0.8 4.4 4.9 4.4
Mitochondria – 1.1 3.4 4.8 1.4 6.2 0 0 0 4.6 -0.7 3.3 -6.9 -6.7 -6.4
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole – 0.1 -2.1 -3.6 -2.5 -3.5 -2.5 -3.5 -3.8 -4.6 -2.4 -4.0 -1.5 -0.5 -1.0