Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.09 0 – 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.1 0 0 0 0.05 0.05 0.06
Bud 0 0 0.07 – 0 0 0 0 0 0.06 0.07 0.07 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.05 – 0.08 0.05 0.06 0.15 0.05 0.08 0.09 0.09 0.05 0.06 0.05 0.08 0.08 0.1 0 0 0.05 0 0 0
Endoplasmic Reticulum 0.07 0.11 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0.09 0 0.06 0 0 0 0 0 0
Endosome 0.07 0 0.21 – 0.28 0.15 0.08 0.15 0 0 0 0 0 0.11 0.07 0.12 0.22 0.18 0.11 0.12 0.14 0.1 0.08 0.07
Golgi 0.44 0.28 0.17 – 0 0 0 0 0 0 0 0 0.26 0.41 0.21 0.47 0.52 0.51 0.74 0.59 0.55 0.63 0.69 0.72
Mitochondria 0.75 0.69 0.56 – 0.67 0.8 0.85 0.66 0.91 0.9 0.87 0.88 0.81 0.52 0.74 0.12 0.12 0.1 0.08 0.16 0.16 0.14 0.1 0.06
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0
SpindlePole 0 0 0.05 – 0.06 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.07 0.19 – 0.19 0.08 0 0.08 0.05 0.08 0 0 0 0.1 0.05 0.11 0.16 0.13 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 32 3 – 1 0 0 1 0 2 0 0 5 3 4 16 16 8 2 12 1 12 21 22
Bud 2 8 6 – 0 4 5 3 4 9 7 7 6 3 8 7 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 2 2 2 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 1 – 0 2 0 1 0 0 0 0 0 2 1 2 0 2 0 0 0 0 0 1
Cytoplasm 4 33 4 – 3 5 9 24 8 13 9 9 17 26 21 10 10 8 0 5 5 0 0 3
Endoplasmic Reticulum 11 41 0 – 0 0 1 0 5 0 0 0 17 20 23 12 4 5 0 3 0 1 3 5
Endosome 12 13 17 – 10 16 12 23 3 0 0 0 12 50 26 16 27 15 20 52 15 25 35 28
Golgi 74 102 14 – 1 0 1 5 1 1 0 0 92 179 82 63 64 42 135 264 62 168 312 282
Mitochondria 126 253 45 – 24 86 123 102 140 138 88 88 283 229 286 16 15 8 13 70 18 37 43 24
Nucleus 1 0 1 – 0 0 3 4 0 5 3 3 0 2 2 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 – 0 0 0 0 0 0 2 2 1 0 2 3 1 2 0 2 1 0 0 0
Nucleolus 1 0 1 – 0 4 0 0 0 0 1 1 1 0 2 1 2 0 0 0 0 0 0 0
Peroxisomes 0 1 1 – 1 6 4 9 4 0 1 1 1 3 1 0 0 0 3 5 3 14 27 14
SpindlePole 0 2 4 – 2 0 4 10 1 5 2 2 2 2 2 5 5 2 0 2 0 1 1 0
Vac/Vac Membrane 12 25 15 – 7 9 6 12 7 12 3 2 15 42 21 15 20 11 2 22 1 2 3 1
Unique Cell Count 168 368 80 36 108 144 155 154 153 101 100 351 441 384 133 123 83 183 446 114 266 454 391
Labelled Cell Count 263 515 112 49 132 168 196 176 187 117 117 452 561 481 167 165 104 183 446 114 266 454 391


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 7.2 7.5 – 7.4 5.9 5.8 6.7 5.2 4.8 4.6 4.6 7.4 7.7 7.2 9.2 9.5 9.0 6.8 7.1 7.6
Std Deviation (1e-4) 1.0 1.2 1.4 – 1.9 1.3 1.4 2.1 1.3 1.3 1.2 1.2 1.0 1.2 1.2 1.8 1.9 1.9 1.1 1.1 1.3
Intensity Change (Log2) – – – – -0.02 -0.36 -0.37 -0.16 -0.54 -0.64 -0.72 -0.72 -0.03 0.03 -0.06 0.29 0.33 0.26 -0.14 -0.09 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 2.1 2.2 1.5
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 2.4 0 1.0 1.0 1.0 0 0 0 0.7 0.9 1.1
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0.8 -1.1 -2.8 -1.2 -5.0 0 0 0 -5.7 -2.4 -4.1 -1.8 0.1 -0.5
Golgi – 0 -4.5 -4.8 -3.8 -5.0 0 0 0 1.6 3.9 0.8 4.4 4.9 4.4
Mitochondria – 1.1 3.4 4.8 1.4 6.2 0 0 0 4.6 -0.7 3.3 -6.9 -6.7 -6.4
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole – 0.1 -2.1 -3.6 -2.5 -3.5 -2.5 -3.5 -3.8 -4.6 -2.4 -4.0 -1.5 -0.5 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2678 5.4524 4.8882 4.2125 4.3259 4.686 3.9712 4.6361 4.414 3.9614 4.0987 4.237 2.6694 3.4061 3.0126 2.5226 2.6531 2.8153
Actin 0.0325 0.0175 0.0154 0.0101 0.0285 0.0063 0.0184 0.0035 0.0248 0.0255 0.0037 0.003 0.0187 0.0068 0.0028 0.0006 0.001 0.0111
Bud 0.0006 0.0014 0.0004 0.0002 0.0004 0.0002 0.0004 0.0002 0.0007 0.0002 0.0005 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0002
Bud Neck 0.0015 0.0011 0.0022 0.0014 0.0177 0.0004 0.0009 0.0002 0.0012 0.0008 0.0003 0.0004 0.0008 0.0002 0.0002 0.0001 0.0001 0.0004
Bud Periphery 0.0008 0.0014 0.0004 0.0001 0.0004 0.0002 0.0004 0.0001 0.0008 0.0002 0.0015 0.0002 0.0001 0.0003 0.0001 0 0 0.0001
Bud Site 0.0029 0.0096 0.0024 0.0004 0.0012 0.0004 0.0037 0.0049 0.004 0.0013 0.0005 0.0001 0.0004 0.0006 0.0002 0.0001 0.0001 0.0002
Cell Periphery 0.0003 0.0004 0.0006 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.0081 0.0155 0.0061 0.0033 0.0038 0.0057 0.0125 0.0077 0.0051 0.0068 0.005 0.0031 0.0059 0.0088 0.0076 0.0068 0.0099 0.0072
Cytoplasmic Foci 0.1154 0.1356 0.0822 0.0768 0.1003 0.0648 0.0955 0.1072 0.0869 0.0848 0.0743 0.063 0.0851 0.1175 0.0923 0.0811 0.0847 0.0771
Eisosomes 0.0004 0.0002 0.0006 0 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.007 0.0016 0.0016 0.0009 0.0011 0.0036 0.0029 0.0007 0.0011 0.0017 0.0037 0.0011 0.0013 0.0008 0.0012 0.0009 0.0012 0.0011
Endosome 0.251 0.3068 0.2766 0.3298 0.2944 0.2502 0.232 0.2254 0.2216 0.3133 0.3382 0.1788 0.356 0.3122 0.3155 0.4573 0.3967 0.2328
Golgi 0.4051 0.4269 0.5138 0.5414 0.4382 0.5292 0.553 0.6257 0.6042 0.4731 0.4858 0.7315 0.4858 0.5301 0.554 0.4401 0.4568 0.6163
Lipid Particles 0.0562 0.016 0.0092 0.0051 0.0145 0.0265 0.0082 0.0023 0.0044 0.0338 0.008 0.0011 0.0049 0.0035 0.0018 0.0022 0.0029 0.006
Mitochondria 0.0556 0.036 0.0565 0.0216 0.0542 0.0807 0.0404 0.0155 0.0281 0.0398 0.0508 0.0142 0.0252 0.0103 0.0179 0.0039 0.0394 0.0368
None 0.0118 0.0056 0.0121 0.0001 0.0021 0.0113 0.0033 0.0007 0.0002 0.0005 0.0008 0 0.001 0.0001 0.0001 0.0001 0.0005 0.003
Nuclear Periphery 0.003 0.0004 0.0005 0.0001 0.0003 0.002 0.0047 0.0002 0.0023 0.0005 0.0112 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0003
Nucleolus 0.0006 0.0005 0.0004 0 0.007 0.0003 0.0007 0.0001 0.0002 0.0009 0.0001 0 0.0001 0 0 0 0 0
Nucleus 0.0007 0.0004 0.0004 0.0001 0.0028 0.0006 0.0036 0.0001 0.0004 0.0003 0.0005 0.0001 0.0001 0 0 0 0 0.0001
Peroxisomes 0.0324 0.0166 0.013 0.0047 0.016 0.0095 0.0082 0.002 0.0057 0.0065 0.0022 0.0011 0.0056 0.0055 0.0028 0.0011 0.0014 0.0037
Punctate Nuclear 0.0079 0.0005 0.0012 0.0001 0.0097 0.0021 0.0024 0.0015 0.0009 0.0022 0.0004 0 0.0035 0.0001 0.0001 0.0001 0.0001 0.0002
Vacuole 0.0036 0.0039 0.0025 0.0013 0.0041 0.0031 0.0039 0.0008 0.0046 0.0046 0.0042 0.0007 0.0024 0.0013 0.0015 0.002 0.0023 0.0014
Vacuole Periphery 0.0027 0.0022 0.0021 0.0023 0.003 0.0028 0.0047 0.001 0.0027 0.003 0.0082 0.0013 0.0027 0.0015 0.0017 0.003 0.0025 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3308 18.0053 13.9416 22.3045 21.005 18.8175 24.1416 26.1771 21.6118 24.7686
Translational Efficiency 0.9779 1.0509 1.1257 0.7193 0.7153 0.9956 0.8475 0.5628 0.7984 0.8395

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
Localization
Cell Percentages Golgi (17%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cog6

Cog6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cog6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available