Standard name
Description Cyclin-like component of the RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; forms a kinase-cyclin pair in the RNAPII holoenzyme with Ssn3p; required for both entry into and execution of the meiotic program; involved in glucose repression and telomere maintenance; cyclin homolog 35% identical to human cyclin C
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.1 0.08 0.15 0.08 0.08 0.09 0.07 0.09 0.05 0.06 0.13 0.17 0.13 0.11 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0 0 0.13 0.13 0.35 0.56 0.41 0.71 0.51 0.7 0.43 0 0 0 0 0 0.06 0 0 0 0 0.05 0
Nucleus 0.82 0.9 0.85 0.82 0.75 0.75 0.69 0.73 0.62 0.7 0.66 0.63 0.83 0.81 0.83 0.82 0.8 0.77 0.77 0.73 0.67 0.65 0.54 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.09 0.08 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05
Vac/Vac Membrane 0.06 0.07 0 0 0 0 0 0 0 0 0 0.12 0.05 0 0 0.06 0.06 0.08 0.1 0.14 0.13 0.11 0.15 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 1 2 1 3 1 4 1 2 1 0 0 0 0 0 1 3 1 1 1 5 7
Bud Neck 0 0 3 0 3 2 1 2 1 0 0 1 0 0 0 0 0 0 0 0 1 2 0 2
Bud Site 2 0 0 0 1 4 10 7 5 5 9 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 3 19 16 43 18 23 22 22 30 14 19 14 35 34 46 31 42 4 0 4 2 3 4
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 15 10 15 0 0 0 0 0 1
Endosome 2 0 0 2 3 4 0 2 0 0 0 7 1 1 2 5 5 6 2 2 1 1 6 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 2 2 0 0 1 1 4 0
Mitochondria 69 1 3 25 36 78 154 105 220 176 197 129 0 0 0 12 8 23 3 1 3 2 10 7
Nucleus 163 81 162 155 211 170 191 187 194 244 184 186 90 170 211 347 247 323 168 80 143 104 123 129
Nuclear Periphery 0 0 1 0 1 1 1 0 1 0 0 1 0 0 0 1 0 1 1 0 0 1 1 1
Nucleolus 13 0 1 0 0 2 1 0 1 2 2 2 0 0 0 0 2 2 4 5 17 15 18 15
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 13 10 7 2 4 16 2 10 1 5 2 6 5 7 9 3 7 2 6 8 12 11
Vac/Vac Membrane 11 6 1 0 7 3 4 1 9 8 8 37 5 8 8 25 20 35 21 14 27 17 33 25
Unique Cell Count 200 90 190 189 282 226 276 257 312 347 280 297 109 209 254 421 309 417 219 110 215 161 228 222
Labelled Cell Count 269 92 206 210 314 285 393 343 461 476 417 394 113 220 260 460 334 453 219 110 215 161 228 222


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 5.5 4.9 5.2 5.0 4.0 3.8 4.5 3.7 4.0 3.6 4.2 5.8 6.0 5.9 6.6 6.9 6.5 5.5 6.0 6.1
Std Deviation (1e-4) 0.7 1.1 0.8 1.7 1.9 1.1 1.2 1.6 0.8 1.1 0.8 1.0 1.3 1.4 1.4 1.5 1.7 1.6 1.3 1.8 1.7
Intensity Change (Log2) – – – 0.07 0.02 -0.29 -0.39 -0.12 -0.43 -0.3 -0.46 -0.22 0.25 0.3 0.26 0.43 0.49 0.4 0.15 0.29 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 1.7 -0.7 -0.6 -0.5 -1.2 -0.5 -2.1 -1.4 0.8 2.0 1.1 0.3 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 4.3 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Nucleus -0.9 -2.7 -2.5 -4.0 -3.2 -5.5 -3.9 -4.7 -5.4 -0.6 -1.0 -0.6 -0.9 -1.5 -2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.6 -2.3 0 0 0 0 0 0 0 -1.9 -1.9 -2.6 -3.3 -2.1 -4.4
Vacuole 0 0 0 0 0 0 0 0 4.8 0 0 0 3.1 3.2 3.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssn8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available