Standard name | |
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Human Ortholog | |
Description | Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; also enters the nucleus and positively regulates transcription initiation; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; binds to mRNAs under glucose starvation, most often in the 3' UTR; forms cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 1 | 10 | 10 | 12 | 27 | – | 10 | 9 | 6 | 16 | 15 | 91 | 110 | 4 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 0 | 0 | 1 | 1 | 0 | 3 | – | 1 | 3 | 4 | 3 | 6 | 16 | 38 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 0 | 4 | 22 | 24 | 4 | 32 | – | 12 | 9 | 3 | 18 | 17 | 18 | 9 | 31 | 18 | 10 | 0 | 0 | 0 | 2 | 1 | 1 |
Cytoplasm | 242 | 95 | 110 | 246 | 219 | 140 | 230 | – | 115 | 98 | 71 | 96 | 226 | 235 | 165 | 202 | 168 | 155 | 258 | 95 | 121 | 175 | 318 | 331 |
Endoplasmic Reticulum | 11 | 0 | 2 | 17 | 47 | 10 | 58 | – | 24 | 11 | 9 | 39 | 21 | 50 | 39 | 9 | 4 | 5 | 8 | 0 | 7 | 17 | 52 | 40 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 14 | 25 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 1 |
Golgi | 2 | 0 | 0 | 2 | 10 | 1 | 22 | – | 0 | 3 | 0 | 5 | 14 | 104 | 159 | 4 | 1 | 10 | 0 | 0 | 0 | 1 | 1 | 1 |
Mitochondria | 4 | 0 | 0 | 9 | 5 | 43 | 41 | – | 67 | 56 | 72 | 99 | 5 | 2 | 6 | 7 | 6 | 5 | 2 | 0 | 0 | 1 | 8 | 2 |
Nucleus | 0 | 0 | 2 | 1 | 2 | 1 | 1 | – | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 4 | 17 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 1 | 3 | 6 | 7 | 28 | 69 | – | 27 | 19 | 20 | 24 | 1 | 3 | 6 | 3 | 1 | 11 | 3 | 1 | 0 | 2 | 3 | 0 |
Unique Cell Count | 261 | 96 | 117 | 288 | 294 | 215 | 414 | 217 | 175 | 159 | 251 | 274 | 417 | 434 | 240 | 189 | 178 | 283 | 102 | 132 | 204 | 398 | 384 | |
Labelled Cell Count | 274 | 96 | 122 | 315 | 325 | 240 | 485 | 259 | 210 | 188 | 301 | 306 | 539 | 580 | 261 | 204 | 198 | 283 | 102 | 132 | 204 | 398 | 384 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.2 | 13.6 | 11.9 | 13.3 | 14.1 | 9.5 | 12.3 | – | 9.8 | 9.5 | 8.6 | 9.6 | 17.1 | 17.7 | 17.7 | 16.5 | 16.7 | 17.0 | 14.8 | 15.4 | 15.6 |
Std Deviation (1e-4) | 2.5 | 2.4 | 1.6 | 1.8 | 2.0 | 2.1 | 2.1 | – | 1.9 | 2.0 | 1.8 | 2.1 | 2.2 | 2.5 | 2.5 | 2.9 | 2.9 | 3.6 | 2.2 | 2.2 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.25 | -0.32 | 0.05 | – | -0.28 | -0.32 | -0.46 | -0.31 | 0.53 | 0.57 | 0.58 | 0.47 | 0.49 | 0.52 | 0.31 | 0.38 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 2.4 | – | 0 | 0 | 0 | 2.3 | 0 | 5.3 | 5.9 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.6 | 1.7 | 0 | 1.6 | – | 0.9 | 0.7 | 0 | 1.4 | 1.1 | 0 | 0 | 2.8 | 2.0 | 0.9 |
Cytoplasm | -2.4 | -4.5 | -5.8 | -7.7 | – | -7.6 | -7.0 | -8.5 | -10.0 | -3.0 | -7.5 | -10.8 | -2.6 | -1.5 | -1.9 |
Endoplasmic Reticulum | 1.8 | 4.0 | 0 | 3.7 | – | 3.0 | 1.9 | 0 | 3.9 | 2.3 | 3.3 | 2.7 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 6.0 | 7.8 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.2 | 3.5 | – | 6.7 | 6.8 | 8.5 | 7.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.1 | 3.9 | – | 3.0 | 2.6 | 3.0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.4191 | 12.7609 | 11.5561 | 10.6769 | 10.0912 | 11.8397 | 12.4659 | 16.419 | 15.904 | 15.1859 | 13.5813 | 15.0404 | 14.1901 | 16.2632 | 15.8215 | 15.068 | 14.8727 | 15.4731 |
Actin | 0.0063 | 0.0018 | 0.0016 | 0.0011 | 0.0005 | 0.0011 | 0.0145 | 0.0049 | 0.001 | 0.0004 | 0.0127 | 0.0019 | 0.002 | 0.0015 | 0.002 | 0.0042 | 0.0026 | 0.0019 |
Bud | 0.0013 | 0.0004 | 0.0006 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.0006 | 0.0005 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0024 | 0.0008 | 0.0015 | 0.0004 | 0.0009 |
Bud Neck | 0.0004 | 0.0003 | 0.0008 | 0.0009 | 0.0004 | 0.0014 | 0.0007 | 0.0003 | 0.0005 | 0.0006 | 0.0008 | 0.0011 | 0.0003 | 0.0003 | 0.0005 | 0.0007 | 0.0009 | 0.0014 |
Bud Periphery | 0.0008 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0004 | 0.0012 | 0.0001 | 0.0007 |
Bud Site | 0.0009 | 0.0007 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0008 | 0.0013 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0022 | 0.0005 | 0.0006 | 0.0004 | 0.0001 |
Cell Periphery | 0.0006 | 0.0004 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0001 | 0.0003 |
Cytoplasm | 0.7781 | 0.8804 | 0.8491 | 0.7042 | 0.8196 | 0.8168 | 0.7505 | 0.9133 | 0.9001 | 0.9211 | 0.8572 | 0.852 | 0.7892 | 0.9313 | 0.8118 | 0.7444 | 0.8368 | 0.7752 |
Cytoplasmic Foci | 0.0157 | 0.0093 | 0.0122 | 0.0155 | 0.0251 | 0.0168 | 0.019 | 0.0092 | 0.0138 | 0.0098 | 0.0224 | 0.0122 | 0.0181 | 0.0103 | 0.015 | 0.0271 | 0.0193 | 0.0167 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0953 | 0.0505 | 0.0754 | 0.1263 | 0.0172 | 0.0659 | 0.0974 | 0.039 | 0.0224 | 0.0179 | 0.0125 | 0.0592 | 0.1031 | 0.0244 | 0.073 | 0.049 | 0.0689 | 0.0847 |
Endosome | 0.0544 | 0.0304 | 0.0357 | 0.106 | 0.106 | 0.0528 | 0.061 | 0.0177 | 0.0394 | 0.0304 | 0.0426 | 0.0431 | 0.0476 | 0.0167 | 0.0489 | 0.112 | 0.0476 | 0.0565 |
Golgi | 0.013 | 0.0065 | 0.0048 | 0.0137 | 0.0181 | 0.0124 | 0.0106 | 0.004 | 0.0061 | 0.0019 | 0.0085 | 0.0102 | 0.0123 | 0.0044 | 0.009 | 0.0212 | 0.006 | 0.0161 |
Lipid Particles | 0.0034 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0037 | 0.0006 | 0.0001 | 0 | 0.0149 | 0.0007 | 0.0012 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0001 |
Mitochondria | 0.0072 | 0.0102 | 0.0036 | 0.004 | 0.0022 | 0.0239 | 0.0049 | 0.0019 | 0.0022 | 0.0011 | 0.0035 | 0.0096 | 0.0044 | 0.0009 | 0.0232 | 0.0182 | 0.0023 | 0.0372 |
None | 0.0016 | 0.0006 | 0.0007 | 0.0006 | 0.0005 | 0.0008 | 0.005 | 0.0006 | 0.0005 | 0.0006 | 0.0019 | 0.0007 | 0.0071 | 0.0007 | 0.001 | 0.0008 | 0.0005 | 0.0011 |
Nuclear Periphery | 0.0045 | 0.0017 | 0.003 | 0.0033 | 0.0016 | 0.0012 | 0.0109 | 0.0012 | 0.0014 | 0.0017 | 0.0036 | 0.0013 | 0.0034 | 0.0009 | 0.0024 | 0.0042 | 0.0025 | 0.0014 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0016 | 0.0008 | 0.0013 | 0.0011 | 0.0008 | 0.0007 | 0.0027 | 0.0008 | 0.0009 | 0.0013 | 0.0012 | 0.0009 | 0.001 | 0.0007 | 0.001 | 0.0012 | 0.001 | 0.0009 |
Peroxisomes | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0037 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0043 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Vacuole | 0.012 | 0.004 | 0.0077 | 0.0155 | 0.0048 | 0.003 | 0.0111 | 0.0034 | 0.0084 | 0.0091 | 0.0063 | 0.0039 | 0.0064 | 0.0018 | 0.0072 | 0.008 | 0.0081 | 0.003 |
Vacuole Periphery | 0.0022 | 0.0013 | 0.0023 | 0.0053 | 0.0025 | 0.0019 | 0.0029 | 0.0007 | 0.0015 | 0.0029 | 0.0023 | 0.0018 | 0.0017 | 0.0004 | 0.002 | 0.0044 | 0.0021 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.3559 | 70.3197 | 46.5391 | 78.6937 | 86.8291 | 99.8676 | 93.6793 | 94.6787 | 80.5638 | 94.606 |
Translational Efficiency | 1.4391 | 2.0816 | 2.6599 | 1.9006 | 1.6051 | 1.6467 | 1.532 | 1.7627 | 1.8596 | 1.5718 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2083 | 1304 | 250 | 122 | 176 | 2505 | 1826 | 865 | 2259 | 3809 | 2076 | 987 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1098.36 | 1401.74 | 1802.49 | 1674.36 | 1278.52 | 1456.27 | 1643.11 | 1617.56 | 1112.40 | 1437.60 | 1662.30 | 1624.58 |
Standard Deviation | 173.63 | 231.12 | 222.22 | 245.02 | 191.98 | 222.73 | 304.94 | 253.21 | 181.67 | 227.12 | 300.71 | 252.90 |
Intensity Change Log 2 | — | 0.351868 | 0.714640 | 0.608259 | — | 0.187803 | 0.361954 | 0.339344 | — | 0.265942 | 0.535686 | 0.469860 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000294 | 0.000704 | 0.001124 | 0.005773 | 0.001506 | 0.000220 | 0.000442 | 0.003135 | 0.000389 | 0.000386 | 0.000524 | 0.003461 |
Bud Neck | 0.000352 | 0.000384 | 0.004146 | 0.003843 | 0.000635 | 0.000265 | 0.000810 | 0.001568 | 0.000374 | 0.000306 | 0.001212 | 0.001849 |
Bud Site | 0.029667 | 0.021374 | 0.037416 | 0.061238 | 0.058110 | 0.014893 | 0.014207 | 0.055076 | 0.031883 | 0.017112 | 0.017002 | 0.055838 |
Cell Periphery | 0.000558 | 0.000677 | 0.003453 | 0.002851 | 0.001678 | 0.000352 | 0.001156 | 0.003790 | 0.000645 | 0.000464 | 0.001433 | 0.003674 |
Cytoplasm | 0.606249* | 0.762884* | 0.607818* | 0.429914* | 0.473223* | 0.795368* | 0.873450* | 0.720278* | 0.595885* | 0.784247* | 0.841462* | 0.684387* |
Cytoplasmic Foci | 0.224763* | 0.118036 | 0.083658 | 0.180042* | 0.293574* | 0.121376 | 0.028630 | 0.102327 | 0.230124* | 0.120232 | 0.035257 | 0.111933 |
Eisosomes | 0.000012 | 0.000007 | 0.000008 | 0.000017 | 0.000014 | 0.000006 | 0.000003 | 0.000011 | 0.000012 | 0.000006 | 0.000004 | 0.000012 |
Endoplasmic Reticulum | 0.021603 | 0.008711 | 0.014193 | 0.006020 | 0.037148 | 0.009680 | 0.003061 | 0.002702 | 0.022814 | 0.009348 | 0.004401 | 0.003112 |
Endosome | 0.007223 | 0.005874 | 0.021411 | 0.040510 | 0.016235 | 0.003914 | 0.004175 | 0.010378 | 0.007925 | 0.004585 | 0.006250 | 0.014103 |
Golgi | 0.028222 | 0.032392 | 0.051705 | 0.055942 | 0.039127 | 0.023202 | 0.019402 | 0.037738 | 0.029071 | 0.026348 | 0.023292 | 0.039989 |
Lipid Particles | 0.001144 | 0.002988 | 0.027000 | 0.039447 | 0.002001 | 0.001723 | 0.009060 | 0.015160 | 0.001211 | 0.002156 | 0.011220 | 0.018162 |
Mitochondria | 0.005529 | 0.001804 | 0.000037 | 0.000033 | 0.001654 | 0.001262 | 0.000072 | 0.001705 | 0.005227 | 0.001447 | 0.000068 | 0.001498 |
Mitotic Spindle | 0.001498 | 0.002266 | 0.010798 | 0.023985 | 0.009216 | 0.001473 | 0.003852 | 0.005736 | 0.002099 | 0.001744 | 0.004689 | 0.007992 |
None | 0.000959 | 0.001042 | 0.000266 | 0.000059 | 0.000883 | 0.000833 | 0.000810 | 0.000186 | 0.000953 | 0.000905 | 0.000745 | 0.000171 |
Nuclear Periphery | 0.000066 | 0.000057 | 0.000196 | 0.000115 | 0.000075 | 0.000019 | 0.000055 | 0.000074 | 0.000066 | 0.000032 | 0.000072 | 0.000079 |
Nuclear Periphery Foci | 0.000848 | 0.003475 | 0.019469 | 0.007269 | 0.002709 | 0.000602 | 0.007004 | 0.003259 | 0.000993 | 0.001586 | 0.008505 | 0.003754 |
Nucleolus | 0.000046 | 0.000072 | 0.000041 | 0.000042 | 0.000030 | 0.000043 | 0.000045 | 0.000042 | 0.000044 | 0.000053 | 0.000044 | 0.000042 |
Nucleus | 0.001743 | 0.001963 | 0.002914 | 0.002340 | 0.000981 | 0.001661 | 0.003486 | 0.001823 | 0.001684 | 0.001765 | 0.003417 | 0.001887 |
Peroxisomes | 0.000296 | 0.000425 | 0.000315 | 0.002928 | 0.000966 | 0.000303 | 0.000258 | 0.002042 | 0.000348 | 0.000345 | 0.000265 | 0.002152 |
Vacuole | 0.013646 | 0.016161 | 0.094580 | 0.123240 | 0.015992 | 0.009998 | 0.024928 | 0.024437 | 0.013829 | 0.012108 | 0.033316 | 0.036649 |
Vacuole Periphery | 0.055281 | 0.018703 | 0.019452 | 0.014392 | 0.044243 | 0.012809 | 0.005095 | 0.008532 | 0.054421 | 0.014827 | 0.006824 | 0.009257 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.99 | -3.13 | -1.34 | -1.23 | -1.12 | 2.41 | 1.97 | -1.98 | -4.76 | -4.36 | 0.06 | -1.78 | -4.22 | -4.22 | -4.01 |
Bud Neck | -0.83 | -7.70 | -6.21 | -6.15 | 0.20 | 3.88 | -1.80 | -6.38 | -10.56 | -5.55 | 2.52 | -9.67 | -11.30 | -11.83 | -4.33 |
Bud Site | 4.64 | -2.35 | -4.05 | -5.12 | -2.80 | 5.52 | 5.53 | 0.29 | -11.06 | -10.88 | 10.29 | 8.76 | -6.91 | -11.64 | -11.23 |
Cell Periphery | -1.43 | -6.87 | -3.04 | -2.86 | 0.90 | 3.84 | 1.45 | -3.22 | -6.35 | -4.74 | 3.56 | -6.71 | -6.21 | -6.61 | -4.46 |
Cytoplasm | -15.64 | 0.12 | 5.99 | 11.14 | 5.04 | -12.78 | -15.73 | -9.06 | 6.96 | 13.47 | -24.46 | -28.89 | -6.94 | 9.47 | 13.74 |
Cytoplasmic Foci | 16.31 | 18.96 | 2.38 | -3.83 | -5.70 | 9.49 | 14.75 | 10.13 | 2.67 | -12.68 | 20.69 | 39.63 | 16.35 | 1.06 | -13.76 |
Eisosomes | 2.90 | 2.68 | -1.37 | -2.85 | -2.66 | 2.19 | 2.82 | 0.74 | -4.25 | -6.23 | 4.66 | 6.78 | 0.10 | -4.60 | -6.77 |
Endoplasmic Reticulum | 7.04 | 1.83 | 5.90 | 1.25 | 1.85 | 3.36 | 4.20 | 4.25 | 6.35 | 0.55 | 7.77 | 10.74 | 11.91 | 7.06 | 1.56 |
Endosome | 2.46 | -7.09 | -6.41 | -6.67 | -3.34 | 6.26 | 5.99 | 2.65 | -6.54 | -5.87 | 8.03 | 2.72 | -5.30 | -8.47 | -6.50 |
Golgi | -2.26 | -4.47 | -2.87 | -2.35 | -0.19 | 5.05 | 6.07 | 0.50 | -4.94 | -6.05 | 2.27 | 3.88 | -3.66 | -4.71 | -5.55 |
Lipid Particles | -4.13 | -8.50 | -4.51 | -4.29 | -1.23 | 0.92 | -9.41 | -8.86 | -9.25 | -3.77 | -4.67 | -13.90 | -10.22 | -9.62 | -3.73 |
Mitochondria | 3.97 | 11.04 | 11.05 | 2.22 | 0.50 | 0.70 | 2.88 | -0.00 | -0.42 | -1.71 | 7.01 | 11.18 | 4.05 | -0.01 | -1.71 |
Mitotic Spindle | -1.99 | -6.00 | -3.44 | -3.34 | -2.18 | 1.64 | 1.06 | 0.56 | -4.71 | -1.79 | 0.78 | -3.43 | -5.02 | -5.50 | -2.76 |
None | -0.49 | 7.42 | 11.33 | 6.04 | 3.72 | 0.08 | 0.12 | 1.04 | 9.56 | 5.46 | 0.41 | 1.62 | 8.64 | 10.20 | 5.69 |
Nuclear Periphery | 0.39 | -4.57 | -1.96 | -2.58 | 2.25 | 2.03 | 0.67 | 0.01 | -1.41 | -0.46 | 1.94 | -0.44 | -0.39 | -1.40 | -0.20 |
Nuclear Periphery Foci | -6.95 | -8.86 | -5.95 | -3.47 | 4.99 | 3.91 | -5.48 | -0.78 | -5.61 | 5.05 | -3.72 | -12.82 | -6.32 | -4.86 | 6.54 |
Nucleolus | -1.03 | 0.93 | 0.21 | 1.07 | -0.57 | -0.92 | -1.12 | -1.01 | -0.35 | 0.29 | -0.90 | -0.31 | -0.04 | 0.80 | 0.24 |
Nucleus | -0.70 | -3.40 | -0.92 | -0.64 | 0.54 | -3.79 | -10.43 | -3.56 | -1.39 | 5.77 | -0.36 | -6.82 | -1.16 | -1.14 | 5.52 |
Peroxisomes | -0.96 | -0.35 | -3.79 | -3.55 | -3.74 | 1.56 | 1.63 | -2.09 | -5.36 | -5.42 | 0.07 | 1.27 | -6.05 | -6.03 | -6.27 |
Vacuole | -1.39 | -7.90 | -6.73 | -6.55 | -1.36 | 1.74 | -2.17 | -2.05 | -7.47 | 0.06 | 1.56 | -9.82 | -8.12 | -8.77 | -0.97 |
Vacuole Periphery | 13.31 | 9.65 | 9.18 | 1.26 | 1.18 | 4.30 | 5.38 | 4.87 | 3.11 | -2.36 | 16.84 | 20.44 | 17.73 | 4.25 | -1.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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