Standard name
Description Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; also enters the nucleus and positively regulates transcription initiation; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; binds to mRNAs under glucose starvation, most often in the 3' UTR; forms cytoplasmic foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.06 0.07 0.05 0.05 0 0.06 0.05 0.22 0.25 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.08 0.08 0 0.08 0.06 0.05 0 0.07 0.06 0 0 0.13 0.1 0.06 0 0 0 0 0 0
Cytoplasm 0.93 0.99 0.94 0.85 0.74 0.65 0.56 0.53 0.56 0.45 0.38 0.82 0.56 0.38 0.84 0.89 0.87 0.91 0.94 0.92 0.86 0.8 0.86
Endoplasmic Reticulum 0 0 0 0.06 0.16 0.05 0.14 0.11 0.06 0.06 0.16 0.08 0.12 0.09 0 0 0 0 0 0.06 0.09 0.13 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.25 0.37 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.2 0.1 0.31 0.32 0.45 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.13 0.17 0.12 0.11 0.13 0.1 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 10 10 12 27 10 9 6 16 15 91 110 4 5 1 0 0 0 0 0 0
Bud 2 0 0 1 1 0 3 1 3 4 3 6 16 38 1 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 8 0 4 22 24 4 32 12 9 3 18 17 18 9 31 18 10 0 0 0 2 1 1
Cytoplasm 242 95 110 246 219 140 230 115 98 71 96 226 235 165 202 168 155 258 95 121 175 318 331
Endoplasmic Reticulum 11 0 2 17 47 10 58 24 11 9 39 21 50 39 9 4 5 8 0 7 17 52 40
Endosome 0 0 0 0 0 0 2 0 0 0 0 0 14 25 0 1 0 1 0 0 1 4 1
Golgi 2 0 0 2 10 1 22 0 3 0 5 14 104 159 4 1 10 0 0 0 1 1 1
Mitochondria 4 0 0 9 5 43 41 67 56 72 99 5 2 6 7 6 5 2 0 0 1 8 2
Nucleus 0 0 2 1 2 1 1 3 2 1 0 0 0 0 0 0 0 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 2 1 0 0 0 0 0 0 2 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 2 4 0 0 0 0 0 0 0 1 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 4 17 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 3 1 3 6 7 28 69 27 19 20 24 1 3 6 3 1 11 3 1 0 2 3 0
Unique Cell Count 261 96 117 288 294 215 414 217 175 159 251 274 417 434 240 189 178 283 102 132 204 398 384
Labelled Cell Count 274 96 122 315 325 240 485 259 210 188 301 306 539 580 261 204 198 283 102 132 204 398 384


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.2 13.6 11.9 13.3 14.1 9.5 12.3 9.8 9.5 8.6 9.6 17.1 17.7 17.7 16.5 16.7 17.0 14.8 15.4 15.6
Std Deviation (1e-4) 2.5 2.4 1.6 1.8 2.0 2.1 2.1 1.9 2.0 1.8 2.1 2.2 2.5 2.5 2.9 2.9 3.6 2.2 2.2 2.2
Intensity Change (Log2) 0.16 0.25 -0.32 0.05 -0.28 -0.32 -0.46 -0.31 0.53 0.57 0.58 0.47 0.49 0.52 0.31 0.38 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.4 0 0 0 2.3 0 5.3 5.9 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 3.3 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 1.7 0 1.6 0.9 0.7 0 1.4 1.1 0 0 2.8 2.0 0.9
Cytoplasm -2.4 -4.5 -5.8 -7.7 -7.6 -7.0 -8.5 -10.0 -3.0 -7.5 -10.8 -2.6 -1.5 -1.9
Endoplasmic Reticulum 1.8 4.0 0 3.7 3.0 1.9 0 3.9 2.3 3.3 2.7 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 6.0 7.8 0 0 0
Mitochondria 0 0 5.2 3.5 6.7 6.8 8.5 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.1 3.9 3.0 2.6 3.0 2.4 0 0 0 0 0 1.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lsm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available