Standard name
Description Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.12 0.23 0.09 0.13 0.13 0.07 0.12 0.14 0.08 0.1 0.11 0.1 0.06 0.05 0.25 0.16 0.25 0 0 0.08 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.72 0.31 0.55 0.33 0.25 0.18 0.13 0.1 0.13 0.09 0.05 0.67 0.63 0.62 0.17 0.16 0.22 0.31 0.47 0.13 0.44 0.44 0.4
Endoplasmic Reticulum 0 0.18 0.18 0.15 0.19 0.17 0.18 0.11 0.09 0.11 0.07 0 0.11 0.09 0.12 0 0.05 0 0.07 0.12 0.08 0.19 0.2 0.21
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.05 0.08 0 0.06 0.06
Golgi 0.31 0.1 0.4 0.32 0.5 0.39 0.37 0.33 0.29 0.21 0.33 0.3 0.2 0.27 0.24 0.63 0.77 0.67 0.38 0.22 0.51 0.19 0.19 0.17
Mitochondria 0.34 0 0.23 0.25 0.13 0.46 0.48 0.58 0.7 0.73 0.77 0.8 0 0 0 0.11 0 0.06 0.05 0 0.06 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.24 0.17 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 13 31 12 20 24 16 33 30 17 22 27 19 20 17 116 43 72 8 3 12 3 5 9
Bud 1 1 0 1 1 5 2 6 9 6 10 13 5 3 4 18 5 17 1 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 0 1 2 0 7 3 6 1 2 3 2 7 3 8 8 5 0 0 0 0 0 0
Cytoplasm 53 76 43 70 50 46 42 35 22 29 19 13 129 204 205 81 43 64 71 62 21 75 110 102
Endoplasmic Reticulum 5 19 25 19 29 32 42 30 20 24 14 10 22 28 40 21 13 8 15 16 13 33 49 54
Endosome 10 1 3 2 3 6 7 10 4 3 2 4 1 7 4 26 7 3 17 6 13 4 15 15
Golgi 50 11 55 41 77 73 85 91 61 47 70 75 39 89 80 296 213 190 88 29 84 33 47 43
Mitochondria 54 1 31 32 20 85 110 158 148 163 165 201 2 6 3 53 6 17 11 2 10 8 4 8
Nucleus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 3 3 0 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 4 0 0 0 0 3 3 5 4 5 5 8 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 1 2 1 0 0 0 22 3 1 2 1 1 0 1 1
Vac/Vac Membrane 20 1 5 1 4 4 9 8 4 6 0 1 2 8 13 101 66 48 9 5 3 6 9 12
Unique Cell Count 159 105 137 128 153 186 231 274 212 224 215 252 193 324 332 473 276 285 233 134 164 174 248 257
Labelled Cell Count 208 125 193 179 207 279 324 380 310 302 312 357 221 372 369 747 411 427 233 134 164 174 248 257


Cytoplasm, Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.6 14.5 12.8 13.8 14.0 12.5 11.4 11.5 11.4 11.3 10.9 10.8 15.4 14.7 14.4 16.8 20.1 20.2 12.4 12.6 13.1
Std Deviation (1e-4) 2.5 2.8 1.8 2.1 2.3 2.0 2.3 2.5 2.6 2.4 2.5 2.6 2.3 2.5 2.5 3.6 4.8 4.5 2.5 1.7 2.2
Intensity Change (Log2) – – – 0.11 0.13 -0.04 -0.16 -0.16 -0.17 -0.18 -0.22 -0.24 0.27 0.2 0.18 0.4 0.66 0.66 -0.05 -0.02 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.9 -2.1 -2.3 -4.4 -2.8 -2.0 -4.1 -3.2 -3.2 -3.2 -5.1 -5.7 0.5 -1.8 0.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.8 0.2 -1.3 -2.9 -4.5 -4.9 -4.3 -5.4 -7.0 6.4 6.2 6.0 -3.7 -3.7 -2.0
Endoplasmic Reticulum -0.7 0.2 -0.2 0 -2.0 -2.4 -2.0 -3.4 -4.7 -1.8 -3.0 -1.8 -5.4 -4.5 -5.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1.6 0 0
Golgi -1.4 1.7 -0.2 -0.6 -1.4 -2.2 -3.9 -1.5 -2.1 -4.0 -2.7 -3.5 4.7 7.4 5.2
Mitochondria 0.5 -2.1 4.3 4.8 6.7 8.6 9.3 10.0 11.0 -6.4 -7.5 -8.3 -3.4 -6.9 -5.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0.1 0 0 0 0 0 0 0 0.1 4.8 5.1 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.0843 15.1994 14.9854 13.4217 14.0819 14.4924 14.7827 17.2695 16.4167 15.0852 15.9847 16.233 15.0554 18.3961 16.9368 16.6656 16.8073 16.9987
Actin 0.007 0.0035 0.0041 0.0142 0.0061 0.004 0.0228 0.0122 0.0093 0.0231 0.0062 0.0056 0.0144 0.0101 0.0182 0.0092 0.0026 0.0151
Bud 0.0005 0.0019 0.0007 0.0008 0.0003 0.0003 0.0007 0.0009 0.0006 0.0003 0.0009 0.0002 0.0007 0.0017 0.0012 0.0003 0.0026 0.0004
Bud Neck 0.0005 0.0005 0.0005 0.0004 0.0003 0.0006 0.0006 0.0006 0.0004 0.0002 0.0017 0.0006 0.0007 0.0006 0.0022 0.0006 0.0005 0.0007
Bud Periphery 0.0003 0.0011 0.0004 0.0003 0.0005 0.0005 0.0006 0.0002 0.0003 0.0001 0.0021 0.0001 0.0005 0.0005 0.0009 0.0001 0.0005 0.0002
Bud Site 0.0007 0.0044 0.0006 0.0014 0.0003 0.0001 0.0015 0.0058 0.0007 0.0003 0.0014 0.0002 0.0016 0.0046 0.003 0.0002 0.0003 0.0002
Cell Periphery 0.0002 0.0004 0.0001 0.0001 0.0004 0.0002 0.0002 0.0002 0.0001 0 0.0009 0.0001 0.0003 0.0003 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.046 0.1527 0.0828 0.0298 0.0305 0.042 0.0822 0.1926 0.1197 0.0323 0.0655 0.0711 0.0875 0.2053 0.1223 0.0598 0.0842 0.0861
Cytoplasmic Foci 0.0875 0.1256 0.1029 0.0871 0.0778 0.0749 0.1099 0.1537 0.1252 0.1051 0.081 0.104 0.1041 0.1497 0.125 0.0807 0.1105 0.103
Eisosomes 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0047 0.0076 0.0048 0.0032 0.0085 0.0095 0.0095 0.0032 0.0046 0.0014 0.004 0.0093 0.0156 0.0073 0.0065 0.0045 0.0028 0.0058
Endosome 0.3029 0.4069 0.3734 0.4465 0.4392 0.329 0.2958 0.3381 0.3829 0.3876 0.3477 0.2634 0.3623 0.3373 0.3755 0.511 0.4363 0.3425
Golgi 0.5104 0.275 0.4044 0.3852 0.4104 0.4967 0.4326 0.2797 0.3346 0.4376 0.3993 0.528 0.3542 0.2616 0.3169 0.2941 0.3434 0.4244
Lipid Particles 0.0013 0.0016 0.0007 0.0032 0.0004 0.001 0.0039 0.0011 0.0011 0.0006 0.0184 0.0007 0.0081 0.0017 0.0038 0.0058 0.0021 0.0016
Mitochondria 0.0285 0.006 0.012 0.0082 0.0167 0.0307 0.0265 0.0034 0.0119 0.0057 0.0083 0.0103 0.021 0.0076 0.0109 0.0054 0.0034 0.013
None 0.0003 0.0002 0.0002 0.0002 0.0002 0.0002 0.0021 0.0002 0.0003 0.0001 0.0006 0.0004 0.0009 0.0003 0.0008 0.0002 0.0005 0.0003
Nuclear Periphery 0.0005 0.0007 0.0004 0.0005 0.0003 0.0027 0.001 0.0002 0.0004 0.0001 0.0278 0.0006 0.0032 0.0004 0.0006 0.0031 0.0003 0.0004
Nucleolus 0 0.0002 0 0.0004 0 0 0.0001 0 0 0 0.0033 0.0001 0.0002 0.0001 0.0002 0 0.0002 0
Nucleus 0.0001 0.0004 0.0002 0.0004 0.0001 0.0002 0.0003 0.0001 0.0002 0 0.0039 0.0003 0.0007 0.0003 0.0003 0.0003 0.0008 0.0001
Peroxisomes 0.0013 0.001 0.0009 0.0011 0.0002 0.0003 0.0031 0.0014 0.0013 0.0024 0.0008 0.0006 0.008 0.0013 0.0022 0.0006 0.0007 0.0011
Punctate Nuclear 0.0001 0.0001 0.0002 0.0005 0.0001 0.0001 0.0009 0.0001 0.0001 0.0001 0.0025 0.0018 0.0032 0.0001 0.0009 0.0003 0.0001 0.0002
Vacuole 0.0036 0.0064 0.0055 0.0074 0.0029 0.0028 0.0029 0.0037 0.0032 0.0009 0.0144 0.001 0.009 0.0061 0.0043 0.0159 0.004 0.002
Vacuole Periphery 0.0036 0.004 0.005 0.009 0.0047 0.0044 0.0026 0.0027 0.0031 0.0019 0.0092 0.0016 0.0039 0.003 0.0039 0.0078 0.0038 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.7052 30.2022 28.5235 43.9433 58.2399 38.3714 25.8527 36.5371 47.0689 50.3346
Translational Efficiency 3.7524 2.5297 2.6278 2.6614 2.0602 3.2299 2.5491 1.8926 1.8548 1.969

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘