Standard name | |
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Description | Protein required for the transport of Gap1p; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 3 |
Bud Neck | – | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 2 | 0 | 2 | 0 | 9 | 0 | 1 | 3 | 2 | 2 | 3 | 7 | 2 | 8 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | – | 192 | 62 | 89 | 83 | 166 | 132 | 240 | 74 | 45 | 111 | 127 | 81 | 114 | 155 | 183 | 196 | 132 | 19 | 170 | 74 | 52 | 105 | 93 |
Endoplasmic Reticulum | – | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 13 | 10 | 14 | 0 | 1 | 0 | 0 | 1 | 0 |
Endosome | – | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 3 | 41 | 30 | 22 | 0 | 6 | 4 | 1 | 1 | 10 |
Golgi | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 3 |
Mitochondria | – | 3 | 0 | 4 | 14 | 11 | 33 | 11 | 44 | 28 | 60 | 54 | 0 | 0 | 1 | 16 | 7 | 2 | 0 | 9 | 7 | 2 | 3 | 8 |
Nucleus | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 11 | 15 | 11 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 21 | 7 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 0 | 0 | 1 | 2 | 0 | 2 | 4 | 71 | 60 | 29 | 0 | 0 | 1 | 0 | 8 | 16 |
Vac/Vac Membrane | – | 4 | 3 | 0 | 1 | 4 | 0 | 6 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 5 | 14 | 12 | 0 | 2 | 0 | 1 | 1 | 1 |
Unique Cell Count | 202 | 65 | 96 | 91 | 180 | 145 | 261 | 95 | 69 | 154 | 161 | 81 | 120 | 162 | 280 | 287 | 186 | 22 | 203 | 95 | 63 | 138 | 145 | |
Labelled Cell Count | 203 | 66 | 100 | 104 | 191 | 167 | 274 | 120 | 82 | 182 | 190 | 83 | 125 | 175 | 364 | 352 | 235 | 22 | 203 | 95 | 63 | 138 | 145 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 4.3 | 5.3 | 4.9 | 4.0 | 4.5 | 3.5 | 4.7 | 3.7 | 3.9 | 4.0 | 4.3 | 5.8 | 5.5 | 5.1 | 5.6 | 6.8 | 7.6 | 6.6 | 6.0 | 6.2 |
Std Deviation (1e-4) | – | 1.2 | 1.6 | 1.0 | 1.5 | 1.2 | 1.4 | 1.3 | 1.1 | 0.6 | 2.3 | 2.2 | 2.4 | 1.4 | 2.0 | 2.4 | 2.6 | 2.4 | 2.7 | 2.4 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.43 | -0.25 | -0.6 | -0.18 | -0.52 | -0.44 | -0.41 | -0.3 | 0.12 | 0.04 | -0.07 | 0.06 | 0.36 | 0.51 | 0.3 | 0.18 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |