Standard name
Description Protein required for the transport of Gap1p; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.95 0.95 0.93 0.91 0.92 0.91 0.92 0.78 0.65 0.72 0.79 1.0 0.95 0.96 0.65 0.68 0.71 0.9 0.84 0.78 0.84 0.76 0.64
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.1 0.12 0 0 0 0 0 0.07
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0.15 0.06 0.23 0 0.46 0.41 0.39 0.34 0 0 0 0.06 0 0 0 0.05 0.08 0 0 0.06
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0.21 0.16 0 0 0 0 0.06 0.12
Vac/Vac Membrane – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 1 2
Bud – 0 0 0 2 0 1 0 1 3 0 1 0 0 0 0 0 0 0 1 0 0 6 3
Bud Neck – 0 0 0 2 2 1 0 0 0 0 0 0 1 0 0 3 0 0 0 0 0 1 1
Bud Site – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 2 0 2 0 9 0 1 3 2 2 3 7 2 8 2 0 1 1 0 0 0
Cytoplasm – 192 62 89 83 166 132 240 74 45 111 127 81 114 155 183 196 132 19 170 74 52 105 93
Endoplasmic Reticulum – 0 1 2 0 0 0 1 0 0 2 2 0 0 0 13 10 14 0 1 0 0 1 0
Endosome – 1 0 1 0 2 0 1 0 1 0 0 0 3 3 41 30 22 0 6 4 1 1 10
Golgi – 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 3 1 1 1 3
Mitochondria – 3 0 4 14 11 33 11 44 28 60 54 0 0 1 16 7 2 0 9 7 2 3 8
Nucleus – 0 0 1 0 0 0 1 0 2 1 0 0 1 1 1 1 0 0 1 0 1 0 0
Nuclear Periphery – 0 0 0 2 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 1 0 0 0 0 0 0 0 0 0 0 1 11 15 11 0 0 0 0 1 0
Peroxisomes – 1 0 0 0 1 0 0 0 0 0 0 0 1 0 21 7 11 0 0 0 0 0 0
SpindlePole – 0 0 0 0 3 0 4 0 0 1 2 0 2 4 71 60 29 0 0 1 0 8 16
Vac/Vac Membrane – 4 3 0 1 4 0 6 1 1 2 1 0 0 2 5 14 12 0 2 0 1 1 1
Unique Cell Count 202 65 96 91 180 145 261 95 69 154 161 81 120 162 280 287 186 22 203 95 63 138 145
Labelled Cell Count 203 66 100 104 191 167 274 120 82 182 190 83 125 175 364 352 235 22 203 95 63 138 145


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.3 5.3 4.9 4.0 4.5 3.5 4.7 3.7 3.9 4.0 4.3 5.8 5.5 5.1 5.6 6.8 7.6 6.6 6.0 6.2
Std Deviation (1e-4) – 1.2 1.6 1.0 1.5 1.2 1.4 1.3 1.1 0.6 2.3 2.2 2.4 1.4 2.0 2.4 2.6 2.4 2.7 2.4 2.2
Intensity Change (Log2) – – – -0.12 -0.43 -0.25 -0.6 -0.18 -0.52 -0.44 -0.41 -0.3 0.12 0.04 -0.07 0.06 0.36 0.51 0.3 0.18 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –