Standard name | |
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Human Ortholog | |
Description | Protein required for the transport of Gap1p; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 3 |
Bud Neck | – | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 2 | 0 | 2 | 0 | 9 | 0 | 1 | 3 | 2 | 2 | 3 | 7 | 2 | 8 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | – | 192 | 62 | 89 | 83 | 166 | 132 | 240 | 74 | 45 | 111 | 127 | 81 | 114 | 155 | 183 | 196 | 132 | 19 | 170 | 74 | 52 | 105 | 93 |
Endoplasmic Reticulum | – | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 13 | 10 | 14 | 0 | 1 | 0 | 0 | 1 | 0 |
Endosome | – | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 3 | 41 | 30 | 22 | 0 | 6 | 4 | 1 | 1 | 10 |
Golgi | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 3 |
Mitochondria | – | 3 | 0 | 4 | 14 | 11 | 33 | 11 | 44 | 28 | 60 | 54 | 0 | 0 | 1 | 16 | 7 | 2 | 0 | 9 | 7 | 2 | 3 | 8 |
Nucleus | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 11 | 15 | 11 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 21 | 7 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 0 | 0 | 1 | 2 | 0 | 2 | 4 | 71 | 60 | 29 | 0 | 0 | 1 | 0 | 8 | 16 |
Vac/Vac Membrane | – | 4 | 3 | 0 | 1 | 4 | 0 | 6 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 5 | 14 | 12 | 0 | 2 | 0 | 1 | 1 | 1 |
Unique Cell Count | 202 | 65 | 96 | 91 | 180 | 145 | 261 | 95 | 69 | 154 | 161 | 81 | 120 | 162 | 280 | 287 | 186 | 22 | 203 | 95 | 63 | 138 | 145 | |
Labelled Cell Count | 203 | 66 | 100 | 104 | 191 | 167 | 274 | 120 | 82 | 182 | 190 | 83 | 125 | 175 | 364 | 352 | 235 | 22 | 203 | 95 | 63 | 138 | 145 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 4.3 | 5.3 | 4.9 | 4.0 | 4.5 | 3.5 | 4.7 | 3.7 | 3.9 | 4.0 | 4.3 | 5.8 | 5.5 | 5.1 | 5.6 | 6.8 | 7.6 | 6.6 | 6.0 | 6.2 |
Std Deviation (1e-4) | – | 1.2 | 1.6 | 1.0 | 1.5 | 1.2 | 1.4 | 1.3 | 1.1 | 0.6 | 2.3 | 2.2 | 2.4 | 1.4 | 2.0 | 2.4 | 2.6 | 2.4 | 2.7 | 2.4 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.43 | -0.25 | -0.6 | -0.18 | -0.52 | -0.44 | -0.41 | -0.3 | 0.12 | 0.04 | -0.07 | 0.06 | 0.36 | 0.51 | 0.3 | 0.18 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3575 | 2.6995 | 2.7349 | 2.2683 | 2.3351 | 2.8207 | 3.7847 | 5.1881 | 4.9456 | 3.8786 | 5.1777 | 4.4244 | 1.5834 | 2.3821 | 2.2779 | 2.448 | 2.0783 | 2.3849 |
Actin | 0.1094 | 0.0023 | 0.0058 | 0.0148 | 0.0032 | 0.007 | 0.0137 | 0.0004 | 0.0002 | 0.0025 | 0.0004 | 0.0009 | 0.0377 | 0.0003 | 0.0004 | 0.0009 | 0.0002 | 0.0018 |
Bud | 0.0023 | 0.0247 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0011 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0005 | 0.0011 | 0.0001 | 0.0011 | 0.0001 | 0.0004 |
Bud Neck | 0.0098 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.001 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0039 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0105 |
Bud Periphery | 0.0067 | 0.0077 | 0.0003 | 0.0007 | 0.0001 | 0.0004 | 0.0021 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0006 | 0.0011 | 0.0001 | 0.0025 | 0.0001 | 0.0004 |
Bud Site | 0.0132 | 0.0099 | 0.0056 | 0.0002 | 0.0002 | 0.004 | 0.007 | 0.002 | 0.0001 | 0.0004 | 0 | 0 | 0.0044 | 0.0036 | 0.0001 | 0.0002 | 0.0001 | 0.0011 |
Cell Periphery | 0.0009 | 0.0007 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.1863 | 0.4833 | 0.4565 | 0.4912 | 0.6053 | 0.5751 | 0.449 | 0.6006 | 0.4233 | 0.5181 | 0.4563 | 0.6446 | 0.3012 | 0.414 | 0.3864 | 0.3193 | 0.4312 | 0.4868 |
Cytoplasmic Foci | 0.0278 | 0.0163 | 0.0124 | 0.0099 | 0.0207 | 0.0109 | 0.0278 | 0.0105 | 0.0036 | 0.0086 | 0.0043 | 0.0115 | 0.0204 | 0.006 | 0.0058 | 0.0066 | 0.0034 | 0.0073 |
Eisosomes | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0097 | 0.0037 | 0.0016 | 0.0013 | 0.0037 | 0.001 | 0.0047 | 0.0025 | 0.0009 | 0.0007 | 0.0004 | 0.0007 | 0.0017 | 0.0009 | 0.0007 | 0.0007 | 0.0003 | 0.0007 |
Endosome | 0.0322 | 0.0028 | 0.0033 | 0.0022 | 0.0112 | 0.0029 | 0.0349 | 0.0112 | 0.0014 | 0.0083 | 0.0006 | 0.0015 | 0.0134 | 0.0023 | 0.0012 | 0.0043 | 0.0005 | 0.0013 |
Golgi | 0.0158 | 0.0006 | 0.0017 | 0.0007 | 0.0019 | 0.0008 | 0.0094 | 0.0016 | 0.0009 | 0.0002 | 0 | 0.0002 | 0.0032 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0214 | 0.0003 | 0.0031 | 0.0004 | 0.0096 | 0.0006 | 0.0045 | 0.0002 | 0.0113 | 0 | 0 | 0.0002 | 0.0044 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Mitochondria | 0.0076 | 0.0014 | 0.0018 | 0.0006 | 0.0008 | 0.0013 | 0.0379 | 0.0003 | 0.0017 | 0.0002 | 0.0001 | 0.0002 | 0.0028 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0002 |
None | 0.491 | 0.4338 | 0.4929 | 0.4717 | 0.3162 | 0.3897 | 0.3941 | 0.366 | 0.5531 | 0.4535 | 0.5368 | 0.3327 | 0.4768 | 0.5451 | 0.5631 | 0.6593 | 0.562 | 0.4461 |
Nuclear Periphery | 0.004 | 0.0012 | 0.0006 | 0.0007 | 0.0023 | 0.0003 | 0.0018 | 0.0008 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0035 | 0.0007 | 0.0004 | 0.0006 | 0.0001 | 0.0003 |
Nucleolus | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.002 | 0.0005 | 0.001 | 0.0001 | 0 | 0.0005 |
Nucleus | 0.001 | 0.0041 | 0.0011 | 0.0011 | 0.0016 | 0.0012 | 0.0013 | 0.0012 | 0.0008 | 0.001 | 0.0004 | 0.0009 | 0.0994 | 0.0212 | 0.0382 | 0.0016 | 0.0007 | 0.0402 |
Peroxisomes | 0.0523 | 0.0029 | 0.0101 | 0.0015 | 0.0065 | 0.0016 | 0.0017 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0023 | 0.0118 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0002 |
Punctate Nuclear | 0.0039 | 0.0016 | 0.0012 | 0.0015 | 0.0145 | 0.0006 | 0.0012 | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.0028 | 0.0081 | 0.001 | 0.0007 | 0.0008 | 0.0002 | 0.0014 |
Vacuole | 0.0015 | 0.0016 | 0.0008 | 0.0004 | 0.0007 | 0.001 | 0.0041 | 0.0008 | 0.0005 | 0.0038 | 0.0001 | 0.0002 | 0.0026 | 0.001 | 0.0008 | 0.0008 | 0.0002 | 0.0004 |
Vacuole Periphery | 0.001 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.1728 | 36.7728 | 12.8409 | 30.3263 | 16.4709 | 16.9288 | 35.814 | 48.7131 | 34.0964 | 32.2901 |
Translational Efficiency | 0.8723 | 0.7516 | 1.4414 | 0.7054 | 1.0959 | 1.2724 | 0.9753 | 0.6187 | 0.8242 | 0.5696 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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