Standard name
Description Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.99 0.94 0.9 0.82 0.83 0.73 0.73 0.7 0.71 0.99 0.98 0.99 0 0.12 – 0.76 0.81 0.7 0.63 0.64 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.15 0.07 0.29 0.28 0.3 0.23 0 0 0 0 0 – 0.11 0.07 0.16 0.18 0.17 0.1
Nucleus 0 0 0 0 0 0 0.05 0.08 0.06 0.07 0.07 0.05 0 0 0 1.0 0.72 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 1 0 2 1 0 1 0 0 1 0 0 0 0 – 2 1 10 11 12 13
Bud 2 0 0 1 0 3 7 6 11 9 4 14 1 2 1 0 0 – 2 2 1 11 13 17
Bud Neck 0 2 51 20 18 11 24 10 12 11 0 22 0 0 0 0 0 – 1 4 3 4 9 15
Bud Site 0 0 0 0 0 0 1 1 3 2 1 1 0 0 0 0 0 – – – – – – –
Cell Periphery 1 2 13 6 5 2 4 5 3 1 4 3 1 3 0 0 0 – 2 4 17 10 8 15
Cytoplasm 221 312 578 470 480 544 695 534 515 476 350 485 481 524 612 0 4 – 182 263 375 239 296 350
Endoplasmic Reticulum 3 2 1 0 0 0 2 0 0 0 0 1 1 1 1 0 0 – 2 1 2 4 5 4
Endosome 0 0 1 0 0 0 1 1 1 0 0 5 0 0 0 0 2 – 2 4 5 5 2 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 – 1 0 2 0 1 1
Mitochondria 4 3 0 0 7 36 131 48 203 185 151 157 5 1 3 0 0 – 25 21 83 67 76 51
Nucleus 1 0 0 0 13 26 46 51 39 43 34 36 1 0 0 19 23 – 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 – 1 1 0 0 1 0
Nucleolus 0 0 0 0 0 0 2 1 1 2 1 3 1 0 0 0 2 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0
SpindlePole 0 0 1 2 2 3 9 7 10 8 3 18 0 1 0 0 0 – 3 1 6 4 6 11
Vac/Vac Membrane 1 2 1 0 15 23 24 31 25 28 20 27 0 7 1 0 2 – 4 10 4 9 13 19
Unique Cell Count 229 316 587 473 508 603 852 643 701 655 498 687 487 535 616 19 32 240 326 533 383 461 516
Labelled Cell Count 233 324 648 500 541 648 948 697 824 767 569 772 492 539 619 19 34 240 326 533 383 461 516


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.8 4.4 4.0 4.8 3.9 3.9 4.4 3.5 3.4 3.3 3.8 3.8 4.3 3.9 17.9 21.9 – 3.5 3.7 4.0
Std Deviation (1e-4) 0.5 0.7 0.7 0.9 1.0 1.1 0.9 1.3 1.1 1.0 0.8 1.0 1.2 2.1 1.0 8.6 9.5 – 1.1 1.4 1.6
Intensity Change (Log2) – – – -0.13 0.12 -0.18 -0.17 -0.02 -0.33 -0.37 -0.42 -0.22 -0.24 -0.04 -0.19 2.01 2.31 – -0.34 -0.26 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4789 -0.5988 -0.8047 -1.1496 -1.6289 -0.6705 -0.7421 -0.3367 -0.5597 -0.8016 -0.7844 -0.599 -0.3661 -0.3683 -0.6132 -0.5454 -0.238 -0.5565
Actin 0.0151 0.0004 0.0076 0 0.0105 0.0026 0.0377 0.0002 0.005 0.0002 0.013 0.0008 0.0218 0.0101 0.0074 0.0125 0.0096 0.0013
Bud 0.0008 0.0032 0.0002 0 0.0001 0.0002 0.0004 0.0001 0.0017 0.0005 0.0015 0.0001 0.0036 0.0042 0.0018 0.0002 0.0005 0.0005
Bud Neck 0.0011 0.0006 0.0002 0.0002 0.0001 0.0113 0.0002 0.0001 0.0001 0.0002 0.0006 0.0004 0.001 0.0008 0.0012 0.0003 0.0004 0.0007
Bud Periphery 0.0015 0.0032 0.0004 0 0.0001 0.0002 0.0007 0.0001 0.0036 0.0009 0.0023 0.0001 0.0076 0.0024 0.0029 0.0005 0.0016 0.005
Bud Site 0.012 0.0112 0.0009 0 0.0002 0.0005 0.0032 0.0023 0.0006 0.0003 0.0024 0.0001 0.0037 0.0171 0.0079 0.0003 0.0012 0.0001
Cell Periphery 0.0036 0.0004 0.0001 0 0.0001 0.0001 0.0003 0.0003 0.0003 0.0001 0.0004 0.0001 0.0002 0.0005 0.0002 0 0.0001 0.0001
Cytoplasm 0.0414 0.0824 0.0847 0.1148 0.0362 0.0978 0.078 0.1556 0.0901 0.1261 0.0745 0.208 0.125 0.2051 0.1795 0.2012 0.1514 0.2267
Cytoplasmic Foci 0.0242 0.0051 0.0149 0.0041 0.0055 0.0078 0.007 0.0042 0.0035 0.0196 0.022 0.0044 0.0255 0.0056 0.0159 0.0327 0.0357 0.0067
Eisosomes 0.0004 0.0002 0.0002 0 0.0001 0.0001 0.0005 0.0002 0.0002 0.0002 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0093 0.002 0.0032 0.002 0.0003 0.0024 0.0029 0.0012 0.0011 0.0007 0.0014 0.0008 0.0057 0.0033 0.0027 0.0014 0.0068 0.0015
Endosome 0.0238 0.0023 0.0168 0.0052 0.0015 0.0224 0.0117 0.0004 0.0016 0.0023 0.0154 0.002 0.0271 0.0027 0.0056 0.0021 0.0522 0.0026
Golgi 0.0044 0.0003 0.0033 0.0001 0.0069 0.0073 0.009 0.0001 0.0022 0.0004 0.013 0.0055 0.0054 0.0022 0.0021 0.0014 0.0124 0.0006
Lipid Particles 0.0118 0.0009 0.0037 0.0002 0.0073 0.0035 0.0124 0.0002 0.0015 0.0076 0.0018 0.0005 0.0259 0.0013 0.0018 0.0088 0.0083 0.0023
Mitochondria 0.0042 0.0006 0.003 0.0003 0.0084 0.0064 0.011 0.0001 0.0014 0.0015 0.0105 0.0016 0.008 0.002 0.0072 0.0006 0.0194 0.0007
None 0.7555 0.8324 0.8112 0.7649 0.9048 0.791 0.7909 0.7998 0.8676 0.7562 0.8343 0.7515 0.5707 0.6539 0.5805 0.6058 0.4888 0.6472
Nuclear Periphery 0.0191 0.0046 0.0029 0.0029 0.0001 0.0023 0.0098 0.0012 0.0012 0.0007 0.001 0.0012 0.0224 0.0046 0.0072 0.0016 0.0191 0.0028
Nucleolus 0.0016 0.0008 0.0007 0.0002 0 0.0011 0.0004 0.0002 0.0003 0.0006 0.0002 0.0002 0.0025 0.0013 0.0017 0.0008 0.0008 0.0008
Nucleus 0.053 0.0437 0.0352 0.099 0.0001 0.0371 0.0162 0.032 0.0142 0.0788 0.0006 0.0154 0.1052 0.0708 0.1568 0.1107 0.157 0.0871
Peroxisomes 0.0064 0.0005 0.0051 0.0001 0.0172 0.0003 0.0031 0.0001 0.0011 0.0011 0.0011 0.0007 0.0115 0.0021 0.0035 0.0124 0.0222 0.0033
Punctate Nuclear 0.0063 0.0033 0.0029 0.0017 0.0002 0.0035 0.0016 0.0007 0.0014 0.0011 0.0018 0.0061 0.0204 0.0076 0.0116 0.0054 0.0032 0.0091
Vacuole 0.0032 0.0014 0.0022 0.0035 0.0001 0.0013 0.0015 0.0007 0.0008 0.0007 0.0012 0.0006 0.0038 0.0018 0.0017 0.0008 0.0047 0.0006
Vacuole Periphery 0.0014 0.0006 0.0005 0.0008 0.0001 0.0008 0.0014 0 0.0003 0.0002 0.0007 0.0001 0.0029 0.0005 0.0007 0.0001 0.0043 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.7269 4.1444 3.4171 3.3553 2.6715 5.1484 4.1904 2.9854 4.347 3.3412
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –