Standard name
Human Ortholog
Description Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.99 0.94 0.9 0.82 0.83 0.73 0.73 0.7 0.71 0.99 0.98 0.99 0 0.12 0.76 0.81 0.7 0.63 0.64 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.15 0.07 0.29 0.28 0.3 0.23 0 0 0 0 0 0.11 0.07 0.16 0.18 0.17 0.1
Nucleus 0 0 0 0 0 0 0.05 0.08 0.06 0.07 0.07 0.05 0 0 0 1.0 0.72 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 1 0 2 1 0 1 0 0 1 0 0 0 0 2 1 10 11 12 13
Bud 2 0 0 1 0 3 7 6 11 9 4 14 1 2 1 0 0 2 2 1 11 13 17
Bud Neck 0 2 51 20 18 11 24 10 12 11 0 22 0 0 0 0 0 1 4 3 4 9 15
Bud Site 0 0 0 0 0 0 1 1 3 2 1 1 0 0 0 0 0
Cell Periphery 1 2 13 6 5 2 4 5 3 1 4 3 1 3 0 0 0 2 4 17 10 8 15
Cytoplasm 221 312 578 470 480 544 695 534 515 476 350 485 481 524 612 0 4 182 263 375 239 296 350
Endoplasmic Reticulum 3 2 1 0 0 0 2 0 0 0 0 1 1 1 1 0 0 2 1 2 4 5 4
Endosome 0 0 1 0 0 0 1 1 1 0 0 5 0 0 0 0 2 2 4 5 5 2 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 2 0 1 1
Mitochondria 4 3 0 0 7 36 131 48 203 185 151 157 5 1 3 0 0 25 21 83 67 76 51
Nucleus 1 0 0 0 13 26 46 51 39 43 34 36 1 0 0 19 23 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 1 1 0 0 1 0
Nucleolus 0 0 0 0 0 0 2 1 1 2 1 3 1 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 1 2 2 3 9 7 10 8 3 18 0 1 0 0 0 3 1 6 4 6 11
Vac/Vac Membrane 1 2 1 0 15 23 24 31 25 28 20 27 0 7 1 0 2 4 10 4 9 13 19
Unique Cell Count 229 316 587 473 508 603 852 643 701 655 498 687 487 535 616 19 32 240 326 533 383 461 516
Labelled Cell Count 233 324 648 500 541 648 948 697 824 767 569 772 492 539 619 19 34 240 326 533 383 461 516


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.8 4.4 4.0 4.8 3.9 3.9 4.4 3.5 3.4 3.3 3.8 3.8 4.3 3.9 17.9 21.9 3.5 3.7 4.0
Std Deviation (1e-4) 0.5 0.7 0.7 0.9 1.0 1.1 0.9 1.3 1.1 1.0 0.8 1.0 1.2 2.1 1.0 8.6 9.5 1.1 1.4 1.6
Intensity Change (Log2) -0.13 0.12 -0.18 -0.17 -0.02 -0.33 -0.37 -0.42 -0.22 -0.24 -0.04 -0.19 2.01 2.31 -0.34 -0.26 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4789 -0.5988 -0.8047 -1.1496 -1.6289 -0.6705 -0.7421 -0.3367 -0.5597 -0.8016 -0.7844 -0.599 -0.3661 -0.3683 -0.6132 -0.5454 -0.238 -0.5565
Actin 0.0151 0.0004 0.0076 0 0.0105 0.0026 0.0377 0.0002 0.005 0.0002 0.013 0.0008 0.0218 0.0101 0.0074 0.0125 0.0096 0.0013
Bud 0.0008 0.0032 0.0002 0 0.0001 0.0002 0.0004 0.0001 0.0017 0.0005 0.0015 0.0001 0.0036 0.0042 0.0018 0.0002 0.0005 0.0005
Bud Neck 0.0011 0.0006 0.0002 0.0002 0.0001 0.0113 0.0002 0.0001 0.0001 0.0002 0.0006 0.0004 0.001 0.0008 0.0012 0.0003 0.0004 0.0007
Bud Periphery 0.0015 0.0032 0.0004 0 0.0001 0.0002 0.0007 0.0001 0.0036 0.0009 0.0023 0.0001 0.0076 0.0024 0.0029 0.0005 0.0016 0.005
Bud Site 0.012 0.0112 0.0009 0 0.0002 0.0005 0.0032 0.0023 0.0006 0.0003 0.0024 0.0001 0.0037 0.0171 0.0079 0.0003 0.0012 0.0001
Cell Periphery 0.0036 0.0004 0.0001 0 0.0001 0.0001 0.0003 0.0003 0.0003 0.0001 0.0004 0.0001 0.0002 0.0005 0.0002 0 0.0001 0.0001
Cytoplasm 0.0414 0.0824 0.0847 0.1148 0.0362 0.0978 0.078 0.1556 0.0901 0.1261 0.0745 0.208 0.125 0.2051 0.1795 0.2012 0.1514 0.2267
Cytoplasmic Foci 0.0242 0.0051 0.0149 0.0041 0.0055 0.0078 0.007 0.0042 0.0035 0.0196 0.022 0.0044 0.0255 0.0056 0.0159 0.0327 0.0357 0.0067
Eisosomes 0.0004 0.0002 0.0002 0 0.0001 0.0001 0.0005 0.0002 0.0002 0.0002 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0093 0.002 0.0032 0.002 0.0003 0.0024 0.0029 0.0012 0.0011 0.0007 0.0014 0.0008 0.0057 0.0033 0.0027 0.0014 0.0068 0.0015
Endosome 0.0238 0.0023 0.0168 0.0052 0.0015 0.0224 0.0117 0.0004 0.0016 0.0023 0.0154 0.002 0.0271 0.0027 0.0056 0.0021 0.0522 0.0026
Golgi 0.0044 0.0003 0.0033 0.0001 0.0069 0.0073 0.009 0.0001 0.0022 0.0004 0.013 0.0055 0.0054 0.0022 0.0021 0.0014 0.0124 0.0006
Lipid Particles 0.0118 0.0009 0.0037 0.0002 0.0073 0.0035 0.0124 0.0002 0.0015 0.0076 0.0018 0.0005 0.0259 0.0013 0.0018 0.0088 0.0083 0.0023
Mitochondria 0.0042 0.0006 0.003 0.0003 0.0084 0.0064 0.011 0.0001 0.0014 0.0015 0.0105 0.0016 0.008 0.002 0.0072 0.0006 0.0194 0.0007
None 0.7555 0.8324 0.8112 0.7649 0.9048 0.791 0.7909 0.7998 0.8676 0.7562 0.8343 0.7515 0.5707 0.6539 0.5805 0.6058 0.4888 0.6472
Nuclear Periphery 0.0191 0.0046 0.0029 0.0029 0.0001 0.0023 0.0098 0.0012 0.0012 0.0007 0.001 0.0012 0.0224 0.0046 0.0072 0.0016 0.0191 0.0028
Nucleolus 0.0016 0.0008 0.0007 0.0002 0 0.0011 0.0004 0.0002 0.0003 0.0006 0.0002 0.0002 0.0025 0.0013 0.0017 0.0008 0.0008 0.0008
Nucleus 0.053 0.0437 0.0352 0.099 0.0001 0.0371 0.0162 0.032 0.0142 0.0788 0.0006 0.0154 0.1052 0.0708 0.1568 0.1107 0.157 0.0871
Peroxisomes 0.0064 0.0005 0.0051 0.0001 0.0172 0.0003 0.0031 0.0001 0.0011 0.0011 0.0011 0.0007 0.0115 0.0021 0.0035 0.0124 0.0222 0.0033
Punctate Nuclear 0.0063 0.0033 0.0029 0.0017 0.0002 0.0035 0.0016 0.0007 0.0014 0.0011 0.0018 0.0061 0.0204 0.0076 0.0116 0.0054 0.0032 0.0091
Vacuole 0.0032 0.0014 0.0022 0.0035 0.0001 0.0013 0.0015 0.0007 0.0008 0.0007 0.0012 0.0006 0.0038 0.0018 0.0017 0.0008 0.0047 0.0006
Vacuole Periphery 0.0014 0.0006 0.0005 0.0008 0.0001 0.0008 0.0014 0 0.0003 0.0002 0.0007 0.0001 0.0029 0.0005 0.0007 0.0001 0.0043 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.7269 4.1444 3.4171 3.3553 2.6715 5.1484 4.1904 2.9854 4.347 3.3412
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
161 268 163 1170 1647 1429 641 2349 1808 1697 804 3519

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 616.97 745.38 934.24 817.60 620.44 647.89 805.61 816.64 620.13 663.29 831.69 816.96
Standard Deviation 62.85 93.59 113.70 126.56 94.72 87.36 95.57 136.11 92.34 95.25 112.15 133.01
Intensity Change Log 2 0.272776 0.598593 0.406195 0.062457 0.376790 0.396409 0.171151 0.491639 0.401296

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000167 0.000452 0.000336 0.001356 0.000101 0.000520 0.000304 0.000456 0.000107 0.000509 0.000311 0.000755
Bud Neck 0.059953 0.037304 0.001110 0.006532 0.017275 0.052646 0.002407 0.014397 0.021075 0.050223 0.002144 0.011782
Bud Site 0.005091 0.011280 0.005483 0.033225 0.002791 0.016398 0.003278 0.039156 0.002996 0.015590 0.003725 0.037184
Cell Periphery 0.000242 0.000266 0.000333 0.000267 0.000222 0.000216 0.000177 0.000231 0.000224 0.000224 0.000208 0.000243
Cytoplasm 0.387394* 0.252681* 0.185941* 0.416651* 0.433912* 0.327999* 0.384285* 0.535579* 0.429770* 0.316105* 0.344073* 0.496038*
Cytoplasmic Foci 0.265843* 0.356413* 0.053835 0.031270 0.246002* 0.301477* 0.024331 0.010286 0.247769* 0.310153* 0.030313 0.017263
Eisosomes 0.000503 0.000257 0.000197 0.000060 0.000352 0.000261 0.000062 0.000029 0.000365 0.000260 0.000089 0.000039
Endoplasmic Reticulum 0.000206 0.000614 0.003667 0.003587 0.000758 0.000535 0.001410 0.001077 0.000709 0.000548 0.001868 0.001912
Endosome 0.014238 0.046225 0.007917 0.012847 0.011420 0.035249 0.002893 0.001409 0.011671 0.036982 0.003912 0.005212
Golgi 0.001183 0.008448 0.001088 0.007877 0.002378 0.007793 0.000932 0.001050 0.002272 0.007896 0.000963 0.003320
Lipid Particles 0.017744 0.012483 0.007353 0.002449 0.016348 0.013679 0.001888 0.000483 0.016472 0.013490 0.002996 0.001136
Mitochondria 0.001874 0.014182 0.000420 0.003358 0.006658 0.021856 0.000368 0.002120 0.006232 0.020644 0.000378 0.002531
Mitotic Spindle 0.000049 0.007242 0.001208 0.050064 0.000348 0.002427 0.008185 0.019923 0.000321 0.003187 0.006771 0.029944
None 0.001996 0.001796 0.002958 0.005646 0.010138 0.003322 0.007262 0.006776 0.009413 0.003081 0.006390 0.006400
Nuclear Periphery 0.000243 0.000429 0.001411 0.001759 0.000556 0.000482 0.001317 0.001038 0.000528 0.000473 0.001336 0.001278
Nuclear Periphery Foci 0.000143 0.000492 0.001857 0.001570 0.000364 0.000289 0.000518 0.000292 0.000344 0.000321 0.000789 0.000717
Nucleolus 0.000466 0.002470 0.001934 0.000573 0.001940 0.002319 0.000835 0.000541 0.001809 0.002343 0.001058 0.000552
Nucleus 0.184975* 0.124115 0.619412* 0.293609* 0.197070* 0.105818 0.498311* 0.308327* 0.195993* 0.108707 0.522863* 0.303434*
Peroxisomes 0.014065 0.037180 0.000846 0.003079 0.007275 0.033818 0.000282 0.000577 0.007880 0.034349 0.000396 0.001409
Vacuole 0.043051 0.083335 0.102087 0.119291 0.043278 0.070269 0.060811 0.055657 0.043258 0.072333 0.069179 0.076814
Vacuole Periphery 0.000574 0.002338 0.000608 0.004931 0.000814 0.002628 0.000144 0.000597 0.000793 0.002582 0.000238 0.002038

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.33 -2.32 -3.51 -1.65 -1.37 -5.69 -1.58 -5.84 1.29 -0.88 -6.25 -2.26 -6.71 -0.13 -1.64
Bud Neck 1.69 4.89 4.46 5.61 -9.65 -12.01 10.03 4.13 14.27 -6.04 -10.13 11.08 7.06 15.92 -6.90
Bud Site -2.29 -0.56 -4.81 -2.46 -2.30 -9.93 -0.76 -10.66 -3.04 -9.49 -10.33 -1.15 -12.31 -3.39 -9.37
Cell Periphery -0.30 -2.15 1.85 1.23 5.12 0.35 2.82 1.61 1.38 -0.14 0.06 1.24 2.17 1.98 1.36
Cytoplasm 6.13 9.27 3.31 -4.62 -8.91 11.03 8.02 0.84 -10.52 -7.55 12.90 11.48 4.79 -9.55 -8.68
Cytoplasmic Foci -4.22 13.51 16.03 23.16 5.18 -6.86 39.15 44.46 47.39 5.75 -8.20 40.73 46.56 52.17 6.82
Eisosomes 1.98 2.39 3.51 8.87 9.72 2.43 8.01 9.18 14.52 8.83 2.96 7.88 9.58 15.82 11.09
Endoplasmic Reticulum -2.15 -6.70 -8.16 -5.22 1.90 2.39 -8.11 -10.72 -15.30 2.70 1.07 -10.01 -10.87 -11.62 2.09
Endosome -4.24 1.48 2.16 6.18 1.31 -11.30 9.91 13.26 17.54 5.40 -12.06 8.55 10.88 17.57 2.75
Golgi -3.61 0.46 -1.72 2.66 -1.67 -7.34 3.27 5.90 9.83 0.16 -8.02 3.49 2.95 9.04 -1.05
Lipid Particles 0.70 1.37 2.11 6.17 7.22 1.70 12.51 13.96 11.27 7.02 1.94 10.76 12.60 12.24 8.39
Mitochondria -4.26 3.60 0.24 4.31 -3.23 -6.72 5.73 4.68 10.31 -3.40 -7.20 5.85 4.65 11.05 -4.65
Mitotic Spindle -2.05 -1.42 -8.11 -4.37 -7.65 -3.44 -2.87 -9.32 -7.82 -2.88 -3.82 -2.94 -12.28 -9.75 -5.23
None 0.10 -0.47 -1.81 -3.58 -2.56 4.60 2.62 2.93 -4.30 0.01 4.70 2.91 3.10 -4.50 -0.17
Nuclear Periphery -2.72 -11.54 -21.43 -16.00 -7.21 0.77 -12.31 -16.52 -14.65 -0.12 0.48 -14.25 -23.15 -20.33 -3.46
Nuclear Periphery Foci -3.41 -5.90 -6.82 -4.82 1.44 1.07 -4.53 -2.10 -4.16 3.53 0.20 -6.87 -6.02 -6.59 1.59
Nucleolus -2.72 -4.92 -0.87 2.66 4.74 -0.89 3.76 4.88 6.39 1.61 -1.47 3.02 5.13 7.15 3.30
Nucleus 3.06 -15.11 -8.50 -15.36 11.02 14.44 -23.58 -24.57 -37.71 9.29 14.51 -28.09 -26.12 -41.08 13.41
Peroxisomes -3.30 2.63 2.54 7.51 -0.94 -11.66 9.77 9.66 15.58 -0.53 -12.45 9.49 9.06 17.16 -1.57
Vacuole -4.53 -6.54 -19.63 -13.69 -6.19 -7.96 -9.78 -18.21 -10.48 -0.90 -9.24 -11.76 -28.08 -18.64 -5.53
Vacuole Periphery -3.27 0.07 -2.46 0.86 -2.36 -7.10 8.17 4.40 9.04 -2.20 -7.71 6.29 0.00 6.62 -3.42