Standard name
Human Ortholog
Description Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.09 0 0 0 0.05 0 0
Golgi 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0.12 0.12 0.18 0.23 0.45 0.26 0 0.09 0.05 0.08 0.09 0.11
Mitochondria 0.96 0.92 0.96 0.98 0.95 0.95 0.9 0.96 0.95 0.96 0.93 0.98 0.92 0.87 0.77 0.07 0.12 0.08 0.83 0.76 0.71 0.74 0.76 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleolus 0.1 0 0.05 0 0.09 0.13 0.16 0.13 0.16 0.16 0.1 0.12 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0.05 0.14 0 0 0 0 0 0 0 0 0 0.1 0.09 0 0.18 0.06 0.18 0.06 0 0.15 0.06 0 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.18 0 0 0 0 0 0 0 0 0 0 0 0.07 0.17 0.15 0.22 0.22 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 3 2 1 3 1 0 0 0 2 5 1 0 3 3 1 1 1 2 2 18
Bud 2 2 1 1 0 2 3 5 0 3 4 1 2 1 0 2 3 5 0 1 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 1 0 1 0 0 1 2 1 0 0 0 0 0 0 0 0 1 1
Cell Periphery 1 6 0 4 0 0 0 2 1 1 1 0 8 14 7 14 19 9 0 0 0 0 0 0
Cytoplasm 0 2 1 2 1 4 4 5 5 2 2 1 4 5 9 25 18 13 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 1 0 2 0 1 3 1 4 4 6 0 0 0 0 1 2
Endosome 1 3 0 0 0 0 1 0 0 0 0 0 6 7 7 20 6 14 3 4 2 7 7 6
Golgi 0 9 0 3 7 0 4 2 1 1 1 1 26 44 58 54 97 40 4 17 4 12 20 34
Mitochondria 145 170 78 118 131 229 300 341 220 240 199 203 201 314 245 17 26 12 125 148 65 106 178 204
Nucleus 0 1 0 0 0 1 1 0 0 0 1 0 0 0 0 14 4 3 0 0 0 0 0 0
Nuclear Periphery 7 11 2 2 6 8 14 14 8 10 6 1 1 3 1 17 7 4 1 0 1 2 0 3
Nucleolus 15 6 4 5 12 32 54 46 37 41 21 25 3 2 4 17 9 11 1 0 0 1 3 1
Peroxisomes 3 9 11 1 2 1 4 4 0 2 2 1 22 32 13 41 14 27 9 4 14 8 8 21
SpindlePole 1 0 0 1 0 1 0 1 1 1 2 0 2 2 1 19 5 10 1 0 0 0 0 1
Vac/Vac Membrane 11 34 3 1 5 6 8 3 7 9 2 1 9 25 54 35 48 33 2 13 0 2 6 13
Unique Cell Count 151 184 81 120 138 240 334 354 231 250 213 208 219 359 318 234 216 151 151 195 92 144 233 312
Labelled Cell Count 187 254 101 138 167 288 396 427 282 310 243 234 287 457 401 279 264 191 151 195 92 144 233 312


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 10.3 9.3 12.4 23.0 23.6 23.6 25.5 26.4 27.6 27.4 33.0 13.3 13.7 14.1 10.9 13.1 11.9 8.9 10.3 11.9
Std Deviation (1e-4) 2.2 3.0 3.3 3.3 6.6 8.3 10.0 10.4 11.5 12.6 13.6 16.2 4.8 4.2 4.1 4.2 4.2 4.8 3.0 3.3 3.6
Intensity Change (Log2) 0.41 1.3 1.34 1.34 1.45 1.5 1.57 1.55 1.82 0.51 0.55 0.6 0.22 0.49 0.36 -0.07 0.14 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 2.7 2.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 0
Golgi 0 0 0 0 0 0 0 0 0 3.2 3.3 4.2 4.7 7.3 5.1
Mitochondria 0.9 -0.5 -0.3 -1.8 0 -0.4 -0.1 -0.9 0.6 -1.4 -2.3 -3.9 -15.0 -13.6 -13.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1.0 2.1 2.6 2.1 2.5 2.6 1.4 1.8 0 0 0 0.7 0 0.7
Peroxisomes 0 0 0 0 0 0 0 0 0 -0.9 -1.3 0 0.8 -2.0 0.8
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 1.1 3.0 2.7 3.8 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.8834 41.9449 36.228 35.795 25.7828 37.6385 50.5137 47.9015 46.5719 39.8878 45.5067 46.5086 33.9131 60.1822 51.7433 56.8814 48.6877 51.0757
Actin 0.0764 0.0069 0.0028 0.0062 0.0132 0.0057 0.0031 0.0031 0.0079 0.0167 0.0234 0.0011 0.0374 0.0024 0.0039 0.0002 0.0692 0.0005
Bud 0.0017 0.0003 0.0003 0.0002 0.0011 0.0002 0 0 0.0001 0.0006 0.0001 0 0.001 0.0001 0.0001 0 0.0003 0
Bud Neck 0.002 0.0004 0.0002 0.0001 0.0005 0.0001 0 0 0.0002 0.0002 0 0 0.0004 0.0002 0.0002 0 0.0009 0
Bud Periphery 0.0022 0.0005 0.0004 0.0004 0.0043 0.0004 0 0 0.0002 0.001 0.0001 0.0001 0.0011 0.0001 0.0002 0.0001 0.0005 0
Bud Site 0.0108 0.0036 0.0014 0.0005 0.0006 0.0005 0.0002 0.0001 0.0028 0.0027 0.0003 0.0001 0.004 0.0009 0.0003 0 0.0009 0
Cell Periphery 0.001 0.0016 0.0014 0.0001 0.0008 0.0001 0.0002 0.0002 0.0002 0.0001 0.0002 0.0002 0.0012 0.0023 0.0007 0.0001 0.0009 0.0001
Cytoplasm 0.0034 0 0.0002 0.0032 0.0004 0.0002 0.0001 0 0.0001 0.0004 0.0003 0.0007 0.0044 0 0 0 0.0005 0
Cytoplasmic Foci 0.0451 0.0008 0.0033 0.0082 0.0144 0.001 0.0058 0.0001 0.002 0.0018 0.0102 0.0037 0.0459 0.0001 0.0019 0 0.0062 0
Eisosomes 0.0013 0.0045 0.0007 0.0006 0.0009 0.0002 0.0007 0.0013 0.0004 0.0003 0.0004 0.0003 0.0021 0.0111 0.0134 0.0001 0.001 0.0002
Endoplasmic Reticulum 0.0045 0 0.0001 0.0012 0.0009 0.0008 0 0 0.0001 0.0019 0.0002 0.0015 0.007 0 0 0 0.0022 0
Endosome 0.0102 0.0013 0.0013 0.0053 0.0009 0.0074 0.0007 0.0003 0.0021 0.0093 0.0006 0.0014 0.0278 0.0003 0.0026 0.0001 0.0048 0
Golgi 0.0081 0.0051 0.0025 0.0019 0.0004 0.0041 0.0011 0.0018 0.0014 0.004 0.0015 0.0054 0.0125 0.0004 0.0201 0.0001 0.0075 0.0001
Lipid Particles 0.0661 0.0151 0.0087 0.0021 0.0119 0.0046 0.0135 0.0032 0.0107 0.0055 0.0064 0.0044 0.0526 0.0025 0.0092 0.0007 0.0109 0.0019
Mitochondria 0.5436 0.9218 0.8988 0.9179 0.7925 0.9541 0.9413 0.976 0.9476 0.937 0.9451 0.9666 0.6156 0.9414 0.9001 0.9962 0.8051 0.9925
None 0.0167 0 0.0003 0.0011 0.0012 0.0001 0.0061 0 0.0002 0.001 0.0006 0.0004 0.0125 0 0 0 0.0007 0
Nuclear Periphery 0.0368 0.0002 0.0003 0.0094 0.0557 0.0019 0.0003 0 0.0003 0.0094 0.0001 0.0005 0.0187 0.0001 0.0001 0 0.0009 0
Nucleolus 0.0482 0.0053 0.0062 0.0003 0.0452 0.0002 0.0019 0.0006 0.0014 0.0004 0.0054 0.0023 0.0508 0.002 0.0039 0.0001 0.0628 0.0002
Nucleus 0.0632 0.0005 0.0199 0.0032 0.0196 0.0004 0.0005 0.0001 0.0005 0.0012 0.0003 0.0004 0.008 0.0003 0.0003 0 0.0016 0
Peroxisomes 0.0192 0.0061 0.0049 0.0017 0.006 0.0011 0.0118 0.0019 0.0068 0.0019 0.0019 0.0007 0.0343 0.0025 0.005 0.0003 0.0025 0.0006
Punctate Nuclear 0.0244 0 0.0249 0.0341 0.0013 0.0004 0.0009 0 0.0005 0.0024 0.0002 0.0001 0.0195 0 0 0 0.0004 0
Vacuole 0.0043 0.0051 0.0056 0.0004 0.0055 0.0012 0.0016 0.0011 0.0021 0.0008 0.0005 0.0011 0.008 0.0056 0.0055 0.0003 0.003 0.0004
Vacuole Periphery 0.0108 0.0207 0.0159 0.0018 0.0228 0.0153 0.01 0.01 0.0124 0.0012 0.0022 0.0091 0.0354 0.0279 0.0324 0.0017 0.0173 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 168.5757 98.6376 96.5746 171.1733 109.2829 178.4381 170.3762 164.0746 183.0034 157.2073
Translational Efficiency 1.5082 1.8302 1.4466 1.6086 1.4926 2.0086 1.6471 1.445 1.4744 1.3568

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency