Standard name
Human Ortholog
Description Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0.06 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0.05 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 – 0.14 0.25 0.22 0.36 – 0.38 0.28 0.29 0.26 – 0.06 0.13 0.23 0.18 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0.05 0 0 0.05 0 0 0
Endosome 0.22 0.37 – 0.28 0.36 0.3 0.34 – 0.29 0.33 0.11 0.16 – 0.55 0.65 0.5 0.41 0.41 0.76 0.55 0.49 0.59 0.58 0.68
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.08 – 0 0 0.07 0 – 0.12 0.11 0.39 0.22 – 0.08 0 0 0.09 0 0 0 0 0 0 0
Nucleus 0 0 – 0.05 0 0 0 – 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.31 0.3 – 0.25 0.11 0.1 0.06 – 0 0 0.12 0 – 0.18 0.12 0.14 0.11 0.11 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0.16 – 0.15 0.16 0 0.13 – 0.08 0.11 0.09 0.07 – 0.22 0.25 0.16 0.14 0.09 0 0.06 0.07 0.07 0.08 0
Vac/Vac Membrane 0.61 0.65 – 0.34 0.43 0.44 0.31 – 0.23 0.29 0.18 0.32 – 0.25 0.23 0.51 0.51 0.55 0.17 0.33 0.29 0.16 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 5 1 0 1 – 0 6 0 3 – 0 1 1 1 0 0 0 0 0 1 1
Bud 0 1 – 0 1 2 4 – 0 11 2 5 – 1 2 2 0 0 0 1 0 0 1 1
Bud Neck 0 0 – 0 2 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 2 1 0 0 – 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 1 – 1 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 – 11 38 23 74 – 42 64 30 28 – 6 24 28 27 43 0 0 0 0 4 2
Endoplasmic Reticulum 1 0 – 1 0 1 0 – 0 3 3 4 – 2 2 3 6 9 0 0 1 0 0 0
Endosome 16 34 – 22 55 32 70 – 32 74 11 17 – 59 117 60 61 75 69 56 12 12 93 141
Golgi 0 1 – 0 0 0 1 – 0 0 0 0 – 1 0 2 6 2 0 0 0 0 1 1
Mitochondria 11 7 – 3 0 7 4 – 13 24 40 23 – 9 2 5 14 5 0 0 0 0 3 2
Nucleus 1 0 – 4 4 2 4 – 5 2 1 1 – 2 3 0 5 3 0 0 0 0 0 3
Nuclear Periphery 2 0 – 0 0 2 0 – 0 0 0 1 – 0 0 1 0 1 1 0 0 0 1 0
Nucleolus 23 28 – 20 17 10 13 – 3 2 12 4 – 19 21 17 16 20 1 1 0 0 1 3
Peroxisomes 0 0 – 1 0 0 0 – 1 0 0 1 – 0 2 0 1 0 0 0 0 0 0 0
SpindlePole 7 15 – 12 25 3 27 – 9 24 9 7 – 24 46 19 21 17 0 6 1 1 12 3
Vac/Vac Membrane 45 60 – 27 66 46 64 – 25 66 19 34 – 27 41 61 75 101 15 33 7 3 34 41
Unique Cell Count 74 93 79 152 105 205 110 226 103 106 108 181 120 148 185 91 104 26 21 160 208
Labelled Cell Count 109 153 106 209 128 263 133 278 127 129 151 261 199 233 276 91 104 26 21 160 208


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 7.6 – 7.2 8.3 6.5 7.1 – 6.4 6.3 5.8 5.5 – 7.1 7.4 9.2 9.9 9.4 – 6.5 7.7
Std Deviation (1e-4) 0.8 1.6 – 1.6 1.8 1.4 4.1 – 1.6 1.3 1.2 0.8 – 1.3 1.1 2.4 2.6 2.5 – 1.4 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3302 2.1463 1.5653 1.1821 0.3184 1.7176 0.9031 2.8862 2.3291 1.6206 1.1834 2.3183 0.4391 1.3921 1.0147 1.089 1.6258 1.1527
Actin 0.0524 0 0.0042 0 0.0001 0.0003 0.0636 0.0001 0.0022 0 0.0001 0 0.073 0 0.0168 0.0002 0.0008 0.0004
Bud 0.0009 0.0001 0.0003 0 0.0001 0.0002 0.0003 0.0004 0.0002 0 0.0006 0.0002 0.0012 0.0003 0.0005 0.0002 0.0002 0.0002
Bud Neck 0.0027 0.0008 0.0008 0.0002 0.0003 0.0007 0.0012 0.0125 0.0009 0.0004 0.0019 0.0009 0.0026 0.0011 0.0007 0.0009 0.0004 0.0012
Bud Periphery 0.001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0003 0.0004 0.0002 0 0.0008 0.0002 0.0013 0.0003 0.0003 0.0002 0.0002 0.0003
Bud Site 0.0061 0.0006 0.0024 0.0001 0.0006 0.0003 0.0044 0.0148 0.001 0.0002 0.0027 0.0008 0.0152 0.0007 0.0034 0.0004 0.0003 0.0017
Cell Periphery 0.0005 0.0002 0.0003 0.0001 0.0001 0.0003 0.0003 0.0006 0.0004 0.0001 0.0004 0.0002 0.0004 0.0004 0.0002 0.0002 0.0001 0.0003
Cytoplasm 0.014 0.0199 0.0046 0.0008 0.0143 0.0038 0.0063 0.0199 0.0501 0.0024 0.0129 0.0108 0.0093 0.0134 0.0384 0.0064 0.0081 0.008
Cytoplasmic Foci 0.133 0.0962 0.0852 0.0413 0.0443 0.0716 0.1013 0.1005 0.0587 0.0536 0.0484 0.0781 0.1553 0.097 0.0743 0.0272 0.108 0.0743
Eisosomes 0.0003 0 0.0001 0 0 0 0.0008 0 0 0 0 0 0.0005 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0066 0.0013 0.0008 0.0003 0.002 0.0008 0.0022 0.002 0.0015 0.0002 0.0015 0.0013 0.0032 0.0012 0.001 0.0006 0.0005 0.0009
Endosome 0.4276 0.421 0.4704 0.4338 0.5442 0.4941 0.5328 0.337 0.433 0.5061 0.5629 0.5564 0.3596 0.3942 0.3908 0.4373 0.6261 0.4021
Golgi 0.055 0.0096 0.0304 0.0066 0.008 0.0167 0.0246 0.011 0.0307 0.0051 0.0419 0.0156 0.0325 0.0121 0.0246 0.027 0.0601 0.0176
Lipid Particles 0.1074 0.0527 0.0686 0.0163 0.0055 0.0964 0.1083 0.0391 0.0145 0.0086 0.1148 0.0117 0.0581 0.0461 0.062 0.006 0.0343 0.0692
Mitochondria 0.0256 0.0011 0.0308 0.0002 0.0019 0.0058 0.0079 0.0006 0.003 0.0002 0.0076 0.004 0.0421 0.0009 0.0239 0.0798 0.0276 0.0299
None 0.0043 0.0005 0.0011 0 0.0046 0.0007 0.0022 0.0008 0.0035 0.0001 0.0008 0.0253 0.0833 0.0026 0.0215 0.0004 0.0002 0.0002
Nuclear Periphery 0.0049 0.0033 0.0018 0.001 0.0196 0.0113 0.0023 0.0061 0.0029 0.0005 0.0025 0.0032 0.0047 0.0044 0.0022 0.0015 0.0009 0.0024
Nucleolus 0.0019 0.0024 0.0018 0.0003 0.0048 0.0089 0.0016 0.0117 0.0021 0.0014 0.0048 0.0011 0.0027 0.0042 0.0034 0.0007 0.0003 0.0024
Nucleus 0.0013 0.002 0.0009 0.0004 0.0873 0.0028 0.0009 0.0266 0.0021 0.0006 0.0065 0.0013 0.0032 0.0027 0.0131 0.0009 0.0008 0.0022
Peroxisomes 0.0432 0.0073 0.012 0.0008 0.001 0.0025 0.0173 0.0133 0.0167 0.0018 0.0041 0.002 0.008 0.0067 0.0131 0.0011 0.0053 0.0037
Punctate Nuclear 0.0039 0.0005 0.0008 0.0001 0.0032 0.0037 0.0023 0.019 0.0003 0.0002 0.0007 0.0004 0.0483 0.0007 0.0011 0.0002 0.0004 0.0011
Vacuole 0.0897 0.2951 0.2233 0.3459 0.1949 0.1923 0.104 0.3275 0.3158 0.3716 0.1635 0.237 0.0728 0.3218 0.2451 0.3363 0.1118 0.2901
Vacuole Periphery 0.0177 0.0852 0.0591 0.1517 0.0633 0.0866 0.015 0.0562 0.0601 0.0468 0.0207 0.0498 0.023 0.0892 0.0634 0.0723 0.0136 0.0917

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1208 20.5825 13.315 20.0755 15.6916 20.0615 24.0126 24.8506 23.8756 19.868
Translational Efficiency 1.6306 1.5588 1.8378 1.3974 1.5427 1.7642 1.1911 1.4193 1.3103 1.48

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
Localization
Cell Percentages vacuole (17%), endosome (10%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps27

Vps27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available