Standard name
Human Ortholog
Description Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.14 0.25 0.22 0.36 0.38 0.28 0.29 0.26 0.06 0.13 0.23 0.18 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0
Endosome 0.22 0.37 0.28 0.36 0.3 0.34 0.29 0.33 0.11 0.16 0.55 0.65 0.5 0.41 0.41 0.76 0.55 0.49 0.59 0.58 0.68
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.08 0 0 0.07 0 0.12 0.11 0.39 0.22 0.08 0 0 0.09 0 0 0 0 0 0 0
Nucleus 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.31 0.3 0.25 0.11 0.1 0.06 0 0 0.12 0 0.18 0.12 0.14 0.11 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0.16 0.15 0.16 0 0.13 0.08 0.11 0.09 0.07 0.22 0.25 0.16 0.14 0.09 0 0.06 0.07 0.07 0.08 0
Vac/Vac Membrane 0.61 0.65 0.34 0.43 0.44 0.31 0.23 0.29 0.18 0.32 0.25 0.23 0.51 0.51 0.55 0.17 0.33 0.29 0.16 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 5 1 0 1 0 6 0 3 0 1 1 1 0 0 0 0 0 1 1
Bud 0 1 0 1 2 4 0 11 2 5 1 2 2 0 0 0 1 0 0 1 1
Bud Neck 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 1 0 0 1 0 0 0 0
Cell Periphery 0 0 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 11 38 23 74 42 64 30 28 6 24 28 27 43 0 0 0 0 4 2
Endoplasmic Reticulum 1 0 1 0 1 0 0 3 3 4 2 2 3 6 9 0 0 1 0 0 0
Endosome 16 34 22 55 32 70 32 74 11 17 59 117 60 61 75 69 56 12 12 93 141
Golgi 0 1 0 0 0 1 0 0 0 0 1 0 2 6 2 0 0 0 0 1 1
Mitochondria 11 7 3 0 7 4 13 24 40 23 9 2 5 14 5 0 0 0 0 3 2
Nucleus 1 0 4 4 2 4 5 2 1 1 2 3 0 5 3 0 0 0 0 0 3
Nuclear Periphery 2 0 0 0 2 0 0 0 0 1 0 0 1 0 1 1 0 0 0 1 0
Nucleolus 23 28 20 17 10 13 3 2 12 4 19 21 17 16 20 1 1 0 0 1 3
Peroxisomes 0 0 1 0 0 0 1 0 0 1 0 2 0 1 0 0 0 0 0 0 0
SpindlePole 7 15 12 25 3 27 9 24 9 7 24 46 19 21 17 0 6 1 1 12 3
Vac/Vac Membrane 45 60 27 66 46 64 25 66 19 34 27 41 61 75 101 15 33 7 3 34 41
Unique Cell Count 74 93 79 152 105 205 110 226 103 106 108 181 120 148 185 91 104 26 21 160 208
Labelled Cell Count 109 153 106 209 128 263 133 278 127 129 151 261 199 233 276 91 104 26 21 160 208


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 7.6 7.2 8.3 6.5 7.1 6.4 6.3 5.8 5.5 7.1 7.4 9.2 9.9 9.4 6.5 7.7
Std Deviation (1e-4) 0.8 1.6 1.6 1.8 1.4 4.1 1.6 1.3 1.2 0.8 1.3 1.1 2.4 2.6 2.5 1.4 1.4
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3302 2.1463 1.5653 1.1821 0.3184 1.7176 0.9031 2.8862 2.3291 1.6206 1.1834 2.3183 0.4391 1.3921 1.0147 1.089 1.6258 1.1527
Actin 0.0524 0 0.0042 0 0.0001 0.0003 0.0636 0.0001 0.0022 0 0.0001 0 0.073 0 0.0168 0.0002 0.0008 0.0004
Bud 0.0009 0.0001 0.0003 0 0.0001 0.0002 0.0003 0.0004 0.0002 0 0.0006 0.0002 0.0012 0.0003 0.0005 0.0002 0.0002 0.0002
Bud Neck 0.0027 0.0008 0.0008 0.0002 0.0003 0.0007 0.0012 0.0125 0.0009 0.0004 0.0019 0.0009 0.0026 0.0011 0.0007 0.0009 0.0004 0.0012
Bud Periphery 0.001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0003 0.0004 0.0002 0 0.0008 0.0002 0.0013 0.0003 0.0003 0.0002 0.0002 0.0003
Bud Site 0.0061 0.0006 0.0024 0.0001 0.0006 0.0003 0.0044 0.0148 0.001 0.0002 0.0027 0.0008 0.0152 0.0007 0.0034 0.0004 0.0003 0.0017
Cell Periphery 0.0005 0.0002 0.0003 0.0001 0.0001 0.0003 0.0003 0.0006 0.0004 0.0001 0.0004 0.0002 0.0004 0.0004 0.0002 0.0002 0.0001 0.0003
Cytoplasm 0.014 0.0199 0.0046 0.0008 0.0143 0.0038 0.0063 0.0199 0.0501 0.0024 0.0129 0.0108 0.0093 0.0134 0.0384 0.0064 0.0081 0.008
Cytoplasmic Foci 0.133 0.0962 0.0852 0.0413 0.0443 0.0716 0.1013 0.1005 0.0587 0.0536 0.0484 0.0781 0.1553 0.097 0.0743 0.0272 0.108 0.0743
Eisosomes 0.0003 0 0.0001 0 0 0 0.0008 0 0 0 0 0 0.0005 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0066 0.0013 0.0008 0.0003 0.002 0.0008 0.0022 0.002 0.0015 0.0002 0.0015 0.0013 0.0032 0.0012 0.001 0.0006 0.0005 0.0009
Endosome 0.4276 0.421 0.4704 0.4338 0.5442 0.4941 0.5328 0.337 0.433 0.5061 0.5629 0.5564 0.3596 0.3942 0.3908 0.4373 0.6261 0.4021
Golgi 0.055 0.0096 0.0304 0.0066 0.008 0.0167 0.0246 0.011 0.0307 0.0051 0.0419 0.0156 0.0325 0.0121 0.0246 0.027 0.0601 0.0176
Lipid Particles 0.1074 0.0527 0.0686 0.0163 0.0055 0.0964 0.1083 0.0391 0.0145 0.0086 0.1148 0.0117 0.0581 0.0461 0.062 0.006 0.0343 0.0692
Mitochondria 0.0256 0.0011 0.0308 0.0002 0.0019 0.0058 0.0079 0.0006 0.003 0.0002 0.0076 0.004 0.0421 0.0009 0.0239 0.0798 0.0276 0.0299
None 0.0043 0.0005 0.0011 0 0.0046 0.0007 0.0022 0.0008 0.0035 0.0001 0.0008 0.0253 0.0833 0.0026 0.0215 0.0004 0.0002 0.0002
Nuclear Periphery 0.0049 0.0033 0.0018 0.001 0.0196 0.0113 0.0023 0.0061 0.0029 0.0005 0.0025 0.0032 0.0047 0.0044 0.0022 0.0015 0.0009 0.0024
Nucleolus 0.0019 0.0024 0.0018 0.0003 0.0048 0.0089 0.0016 0.0117 0.0021 0.0014 0.0048 0.0011 0.0027 0.0042 0.0034 0.0007 0.0003 0.0024
Nucleus 0.0013 0.002 0.0009 0.0004 0.0873 0.0028 0.0009 0.0266 0.0021 0.0006 0.0065 0.0013 0.0032 0.0027 0.0131 0.0009 0.0008 0.0022
Peroxisomes 0.0432 0.0073 0.012 0.0008 0.001 0.0025 0.0173 0.0133 0.0167 0.0018 0.0041 0.002 0.008 0.0067 0.0131 0.0011 0.0053 0.0037
Punctate Nuclear 0.0039 0.0005 0.0008 0.0001 0.0032 0.0037 0.0023 0.019 0.0003 0.0002 0.0007 0.0004 0.0483 0.0007 0.0011 0.0002 0.0004 0.0011
Vacuole 0.0897 0.2951 0.2233 0.3459 0.1949 0.1923 0.104 0.3275 0.3158 0.3716 0.1635 0.237 0.0728 0.3218 0.2451 0.3363 0.1118 0.2901
Vacuole Periphery 0.0177 0.0852 0.0591 0.1517 0.0633 0.0866 0.015 0.0562 0.0601 0.0468 0.0207 0.0498 0.023 0.0892 0.0634 0.0723 0.0136 0.0917

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1208 20.5825 13.315 20.0755 15.6916 20.0615 24.0126 24.8506 23.8756 19.868
Translational Efficiency 1.6306 1.5588 1.8378 1.3974 1.5427 1.7642 1.1911 1.4193 1.3103 1.48

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
162 212 182 1275 1380 1276 1630 660 1542 1488 1812 1935

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 707.38 910.85 1053.75 1008.20 795.91 794.96 935.24 1086.42 786.61 811.47 947.14 1034.88
Standard Deviation 73.53 134.70 104.97 165.62 107.60 107.01 176.18 156.02 108.01 118.51 174.06 166.59
Intensity Change Log 2 0.364728 0.574975 0.511225 -0.001723 0.232731 0.448905 0.182334 0.403913 0.478565

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000201 0.000183 0.000901 0.011401 0.000054 0.000173 0.001334 0.004819 0.000070 0.000174 0.001291 0.009156
Bud Neck 0.034733 0.010866 0.049370 0.004206 0.008877 0.009119 0.017420 0.006496 0.011594 0.009368 0.020629 0.004987
Bud Site 0.059637 0.018216 0.037092 0.040861 0.020265 0.012823 0.027063 0.035615 0.024401 0.013591 0.028071 0.039072
Cell Periphery 0.000542 0.000099 0.000106 0.000142 0.000231 0.000141 0.000055 0.000588 0.000264 0.000135 0.000060 0.000294
Cytoplasm 0.008747 0.020738 0.029650 0.019585 0.012753 0.014692 0.033303 0.010200 0.012332 0.015554 0.032936 0.016384
Cytoplasmic Foci 0.070972 0.098787 0.063090 0.194431* 0.068433 0.064194 0.140614 0.176781 0.068700 0.069122 0.132827 0.188411*
Eisosomes 0.000034 0.000019 0.000077 0.000033 0.000027 0.000021 0.000021 0.000029 0.000028 0.000020 0.000027 0.000032
Endoplasmic Reticulum 0.000446 0.000797 0.005276 0.000346 0.000553 0.000985 0.000574 0.000749 0.000541 0.000958 0.001046 0.000484
Endosome 0.611104* 0.494743* 0.316683* 0.448218* 0.583676* 0.506140* 0.470562* 0.468822* 0.586557* 0.504516* 0.455106* 0.455246*
Golgi 0.052162 0.017620 0.004650 0.113202* 0.032979 0.020631 0.015477 0.097520 0.034994 0.020202 0.014390 0.107853
Lipid Particles 0.001083 0.003413 0.006195 0.006551 0.001762 0.002683 0.003711 0.002578 0.001690 0.002787 0.003960 0.005196
Mitochondria 0.008908 0.004409 0.001066 0.006078 0.003343 0.007634 0.001842 0.006494 0.003928 0.007175 0.001764 0.006220
Mitotic Spindle 0.004480 0.019774 0.054353 0.027443 0.003849 0.008844 0.066630 0.034591 0.003915 0.010401 0.065397 0.029881
None 0.000090 0.000406 0.000303 0.000039 0.000379 0.000645 0.000559 0.000034 0.000349 0.000611 0.000534 0.000037
Nuclear Periphery 0.000111 0.000769 0.003320 0.000185 0.000135 0.000266 0.000741 0.000220 0.000133 0.000338 0.001000 0.000197
Nuclear Periphery Foci 0.000731 0.004262 0.018015 0.002258 0.001824 0.001033 0.008258 0.001653 0.001709 0.001493 0.009238 0.002052
Nucleolus 0.000316 0.003048 0.023234 0.000147 0.001472 0.003517 0.005943 0.000263 0.001351 0.003450 0.007680 0.000186
Nucleus 0.000460 0.001560 0.046322 0.000166 0.001547 0.003674 0.006628 0.000173 0.001433 0.003373 0.010615 0.000168
Peroxisomes 0.030160 0.013173 0.036167 0.036349 0.009240 0.008653 0.027131 0.040422 0.011438 0.009297 0.028039 0.037738
Vacuole 0.091407 0.254832* 0.293975* 0.073902 0.195521 0.273536* 0.161875 0.094454 0.184583 0.270871* 0.175144 0.080912
Vacuole Periphery 0.023677 0.032286 0.010155 0.014457 0.053078 0.060597 0.010258 0.017498 0.049989 0.056564 0.010248 0.015494

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.07 -3.91 -5.32 -5.32 -4.73 -1.52 -2.02 -3.36 -3.27 -2.23 -1.58 -2.22 -6.19 -6.10 -4.94
Bud Neck 3.27 -2.18 4.53 2.40 7.84 -0.28 -6.46 1.37 1.67 7.44 1.59 -6.38 5.52 4.62 12.13
Bud Site 4.00 1.76 1.66 -5.35 -0.29 3.59 -3.82 -4.28 -6.34 -2.31 4.96 -2.48 -5.60 -10.71 -4.11
Cell Periphery 2.74 2.64 2.53 -1.22 -0.87 2.11 5.65 -0.76 -0.95 -1.13 3.13 6.20 -0.17 -0.97 -1.44
Cytoplasm -2.78 -3.13 -4.37 -0.15 1.59 -1.14 -8.67 0.80 1.85 9.12 -1.86 -9.21 -2.97 -0.92 7.14
Cytoplasmic Foci -2.18 1.51 -11.89 -9.71 -18.27 1.01 -13.98 -14.97 -15.63 -5.57 -0.16 -13.07 -25.04 -25.18 -11.69
Eisosomes 2.19 -6.94 0.15 -2.45 7.52 0.63 0.23 -0.18 -1.41 -0.76 0.76 -0.40 -0.41 -3.47 -0.06
Endoplasmic Reticulum -1.81 -5.91 0.56 2.23 6.02 -1.74 -2.63 -0.37 0.45 0.13 -2.05 -6.01 0.30 1.84 4.20
Endosome 4.00 12.10 7.11 2.28 -9.27 6.87 14.38 9.35 3.12 -2.50 7.83 17.19 14.58 5.42 -3.88
Golgi 5.33 8.11 -8.32 -19.50 -26.98* 5.83 10.52 -10.99 -13.35 -14.81 7.38 12.64 -19.79 -25.08 -28.46*
Lipid Particles -1.57 -4.86 -4.79 -1.43 0.25 -1.65 -4.43 -1.78 0.13 2.67 -2.12 -5.54 -4.90 -2.95 -1.23
Mitochondria 1.72 3.81 1.27 -0.80 -4.43 -3.92 3.18 -1.54 0.58 -2.30 -3.25 4.56 -2.09 0.79 -4.38
Mitotic Spindle -2.53 -4.82 -7.31 -1.36 2.90 -3.10 -16.10 -7.05 -5.60 6.12 -3.96 -16.80 -11.10 -7.13 8.99
None -1.11 -3.72 2.84 1.21 4.39 -1.17 -1.54 3.10 3.00 4.70 -1.41 -1.75 3.12 3.22 4.97
Nuclear Periphery -1.44 -4.72 -0.95 1.29 4.64 -3.47 -11.21 -2.57 1.00 9.76 -2.83 -10.94 -2.61 1.90 10.32
Nuclear Periphery Foci -1.69 -5.59 -2.84 0.99 5.15 1.48 -6.68 0.23 -1.76 7.19 0.20 -8.25 -0.81 -1.08 8.09
Nucleolus -2.00 -4.69 2.00 2.10 4.72 -3.02 -6.66 4.62 4.96 8.19 -3.42 -8.09 5.04 5.49 9.43
Nucleus -2.33 -5.72 2.03 2.69 5.74 -2.44 -7.20 2.68 4.92 8.93 -2.64 -9.02 2.71 5.27 10.26
Peroxisomes 2.47 -0.15 -0.86 -6.34 -1.01 0.44 -9.06 -8.65 -8.77 -3.85 1.56 -8.14 -11.75 -13.13 -4.63
Vacuole -7.43 -10.13 1.07 9.46 12.69 -7.93 1.87 11.78 18.53 10.74 -9.51 -1.19 15.48 24.28 18.12
Vacuole Periphery -1.36 3.02 1.90 4.36 -3.14 -2.03 17.65 11.93 13.28 -3.99 -1.95 17.78 14.20 15.76 -4.91