Standard name
Description Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.14 0.25 0.22 0.36 0.38 0.28 0.29 0.26 0.06 0.13 0.23 0.18 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0
Endosome 0.22 0.37 0.28 0.36 0.3 0.34 0.29 0.33 0.11 0.16 0.55 0.65 0.5 0.41 0.41 0.76 0.55 0.49 0.59 0.58 0.68
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.08 0 0 0.07 0 0.12 0.11 0.39 0.22 0.08 0 0 0.09 0 0 0 0 0 0 0
Nucleus 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.31 0.3 0.25 0.11 0.1 0.06 0 0 0.12 0 0.18 0.12 0.14 0.11 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0.16 0.15 0.16 0 0.13 0.08 0.11 0.09 0.07 0.22 0.25 0.16 0.14 0.09 0 0.06 0.07 0.07 0.08 0
Vac/Vac Membrane 0.61 0.65 0.34 0.43 0.44 0.31 0.23 0.29 0.18 0.32 0.25 0.23 0.51 0.51 0.55 0.17 0.33 0.29 0.16 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 5 1 0 1 0 6 0 3 0 1 1 1 0 0 0 0 0 1 1
Bud 0 1 0 1 2 4 0 11 2 5 1 2 2 0 0 0 1 0 0 1 1
Bud Neck 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 1 0 0 1 0 0 0 0
Cell Periphery 0 0 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 11 38 23 74 42 64 30 28 6 24 28 27 43 0 0 0 0 4 2
Endoplasmic Reticulum 1 0 1 0 1 0 0 3 3 4 2 2 3 6 9 0 0 1 0 0 0
Endosome 16 34 22 55 32 70 32 74 11 17 59 117 60 61 75 69 56 12 12 93 141
Golgi 0 1 0 0 0 1 0 0 0 0 1 0 2 6 2 0 0 0 0 1 1
Mitochondria 11 7 3 0 7 4 13 24 40 23 9 2 5 14 5 0 0 0 0 3 2
Nucleus 1 0 4 4 2 4 5 2 1 1 2 3 0 5 3 0 0 0 0 0 3
Nuclear Periphery 2 0 0 0 2 0 0 0 0 1 0 0 1 0 1 1 0 0 0 1 0
Nucleolus 23 28 20 17 10 13 3 2 12 4 19 21 17 16 20 1 1 0 0 1 3
Peroxisomes 0 0 1 0 0 0 1 0 0 1 0 2 0 1 0 0 0 0 0 0 0
SpindlePole 7 15 12 25 3 27 9 24 9 7 24 46 19 21 17 0 6 1 1 12 3
Vac/Vac Membrane 45 60 27 66 46 64 25 66 19 34 27 41 61 75 101 15 33 7 3 34 41
Unique Cell Count 74 93 79 152 105 205 110 226 103 106 108 181 120 148 185 91 104 26 21 160 208
Labelled Cell Count 109 153 106 209 128 263 133 278 127 129 151 261 199 233 276 91 104 26 21 160 208


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 7.6 7.2 8.3 6.5 7.1 6.4 6.3 5.8 5.5 7.1 7.4 9.2 9.9 9.4 6.5 7.7
Std Deviation (1e-4) 0.8 1.6 1.6 1.8 1.4 4.1 1.6 1.3 1.2 0.8 1.3 1.1 2.4 2.6 2.5 1.4 1.4
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available