Standard name
Human Ortholog
Description RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.07 0.07 0.06 0.07 – 0.05 0.07 0.07 0.06 0.16 0.2 0.05 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0.33 0.33 0.43 0.68 0.74 0.67 – 0.73 0.77 0.77 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Nucleus 0.94 0.88 0.87 0.9 0.73 0.68 0.66 0.65 – 0.62 0.61 0.61 0.9 0.84 0.77 0.9 0.89 0.92 0.92 0.88 0.89 0.79 0.68 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0.07 0.06 0.05 0 0.05 0.05 0.12 0.2 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 2 5 7 10 8 – 13 9 10 1 0 0 0 0 0 0 2 0 1 0 5
Bud Neck 0 0 0 0 1 1 0 1 – 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 1 22 20 17 – 27 25 28 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 7 5 7 29 40 45 52 – 34 34 47 19 57 89 10 9 4 1 2 1 0 3 2
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 – 0 0 0 2 2 1 5 3 5 0 0 0 1 2 4
Endosome 0 0 0 0 1 0 0 1 – 0 0 0 0 0 0 0 1 0 2 0 1 1 3 5
Golgi 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 1 1 1 2 0 2 0 1 2
Mitochondria 8 15 63 96 171 403 524 481 – 506 399 537 13 2 2 12 9 11 1 1 0 0 7 10
Nucleus 247 117 165 258 290 404 470 469 – 428 317 423 266 307 342 184 146 176 241 121 173 99 210 213
Nuclear Periphery 0 0 1 5 4 2 4 4 – 3 5 9 0 0 0 3 1 0 0 0 0 0 1 1
Nucleolus 1 0 0 1 1 3 7 5 – 4 5 7 0 0 0 1 0 1 0 0 0 1 6 8
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 2 0 4 3 – 9 0 5 2 1 1 1 1 1 2 0 3 0 3 4
Vac/Vac Membrane 2 9 2 2 20 7 15 8 – 13 11 13 4 3 12 14 10 10 7 7 9 14 62 71
Unique Cell Count 263 133 189 287 399 592 710 723 691 520 698 294 367 442 205 164 192 261 139 194 127 311 340
Labelled Cell Count 267 148 238 372 526 889 1099 1049 1037 805 1079 307 372 448 231 181 209 261 139 194 127 311 340


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.3 4.0 3.7 3.8 3.5 3.0 3.3 – 3.0 3.0 2.9 5.9 6.2 6.2 6.9 7.0 7.2 6.6 6.9 7.1
Std Deviation (1e-4) 0.8 1.2 0.8 1.1 1.5 1.0 0.9 1.2 – 0.9 1.2 0.8 1.1 1.7 1.7 1.1 1.6 1.3 1.8 1.9 2.0
Intensity Change (Log2) – – – -0.1 -0.08 -0.2 -0.41 -0.29 – -0.43 -0.4 -0.45 0.56 0.64 0.64 0.79 0.81 0.85 0.71 0.79 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.2 2.1 2.0 2.3 – 1.3 2.0 2.1 1.9 4.6 5.7 1.2 1.4 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -8.5 -11.4 -12.4 -6.9 -6.5 -6.8
Nucleus 0.9 -4.0 -5.1 -5.7 -6.0 – -6.6 -6.6 -6.9 1.1 -1.1 -2.9 0.8 0.5 1.4
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 2.4 0 0 0 – 0 0 0 0 0 0 2.9 2.6 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1414 1.0853 0.7037 0.8089 0.7645 0.961 2.1324 2.9193 2.3982 2.0687 1.7492 2.4698 0.5049 0.5934 0.0829 0.0523 -0.1257 0.0501
Actin 0.0195 0 0.0002 0.0024 0.001 0.0019 0.0061 0 0.0075 0 0.0001 0.0093 0.0026 0.0001 0.0103 0.0177 0 0
Bud 0.0002 0 0.0001 0.0001 0.0009 0 0.0006 0.0002 0.0008 0 0.0006 0.0006 0.0001 0.0001 0.0004 0.0006 0 0.0001
Bud Neck 0.0013 0.0001 0.0001 0.0003 0.004 0.0005 0.001 0.0002 0.0018 0.0002 0.0188 0.0019 0.0001 0.0001 0.0014 0.007 0.0001 0.0024
Bud Periphery 0.0003 0 0.0001 0.0003 0.0019 0 0.0024 0.0001 0.0025 0 0.0006 0.0012 0 0.0002 0.0007 0.0012 0 0.0001
Bud Site 0.0015 0.0001 0 0.0004 0.0139 0 0.0009 0.0001 0.0021 0 0.0352 0.0008 0.0006 0.0001 0.0014 0.0042 0.0001 0.0001
Cell Periphery 0.0001 0 0 0.0001 0.0004 0 0.0002 0 0.0008 0 0.0006 0.0002 0 0 0.0003 0.0006 0 0
Cytoplasm 0.0044 0.0009 0.0011 0.0005 0.0022 0.0001 0.0182 0.0192 0.0034 0.0014 0.0043 0.0014 0.0013 0.0004 0.0017 0.0008 0 0.0001
Cytoplasmic Foci 0.0069 0 0.0009 0.0012 0.0012 0.0002 0.0313 0.0003 0.0043 0.0001 0.0004 0.0013 0.1126 0 0.0051 0.0274 0 0
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0.0007 0 0 0.0001 0 0 0.0005 0.0004 0 0
Endoplasmic Reticulum 0.0032 0 0.0001 0.0012 0.0001 0.0001 0.0015 0.0004 0.0061 0 0.0001 0.0062 0.0003 0.0003 0.0004 0.0004 0 0
Endosome 0.0122 0.0001 0.0007 0.0138 0.0012 0.0003 0.0054 0.0003 0.0087 0 0.0003 0.0076 0.0083 0.0002 0.0093 0.0359 0 0
Golgi 0.0039 0 0.0003 0.0029 0.0006 0.0001 0.0017 0 0.0019 0 0.0001 0.0076 0.004 0 0.0097 0.0328 0 0
Lipid Particles 0.0118 0 0.0055 0.0051 0.0007 0.0003 0.0048 0.0001 0.0018 0 0 0.0016 0.0229 0 0.0076 0.0452 0 0
Mitochondria 0.0017 0.0001 0.0012 0.0012 0.002 0.0002 0.0097 0.0003 0.0069 0.0002 0.0003 0.0111 0.0004 0.0012 0.0402 0.1132 0 0.0001
None 0.0084 0.0022 0.0031 0.0005 0.0032 0.0002 0.0109 0.0291 0.0067 0.0009 0.0001 0.0007 0.0016 0.0001 0.0282 0.0003 0 0
Nuclear Periphery 0.0211 0.004 0.0094 0.0082 0.0032 0.0078 0.006 0.0034 0.0032 0.0053 0.0014 0.0054 0.0017 0.0175 0.0035 0.0013 0.0001 0.0002
Nucleolus 0.0065 0.0098 0.005 0.0062 0.0171 0.0152 0.0055 0.0299 0.0033 0.0041 0.011 0.0116 0.006 0.0085 0.0035 0.0047 0.0049 0.0274
Nucleus 0.857 0.9749 0.9645 0.9469 0.9159 0.9629 0.8565 0.9094 0.9319 0.9465 0.9233 0.9113 0.7631 0.9682 0.8691 0.6654 0.9947 0.9688
Peroxisomes 0.011 0 0.0024 0.0006 0.0011 0.0039 0.0141 0 0.0012 0 0.0001 0.0007 0.0412 0 0.0034 0.0324 0 0
Punctate Nuclear 0.027 0.0077 0.005 0.0067 0.0277 0.0061 0.0162 0.0059 0.0015 0.0411 0.001 0.0159 0.033 0.0004 0.0009 0.0012 0 0.0001
Vacuole 0.001 0.0001 0.0002 0.0008 0.0015 0.0001 0.0047 0.0009 0.0024 0.0001 0.0017 0.0023 0.0001 0.0009 0.0015 0.0059 0.0001 0.0003
Vacuole Periphery 0.0007 0 0.0002 0.0006 0.0002 0.0001 0.0024 0.0002 0.0006 0.0001 0.0001 0.0009 0.0001 0.0016 0.0008 0.0014 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2107 8.2479 13.6316 12.6975 10.6032 7.1256 13.5516 13.3039 11.552 14.4705
Translational Efficiency 0.9154 1.3371 0.6356 0.718 0.9281 1.4006 0.8271 0.7221 0.774 0.6572

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1723 1070 1536 1403 1549 1441 1882 1702 3272 2511 3418 3105

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 696.48 835.11 882.04 901.10 661.20 761.05 868.72 947.51 679.78 792.61 874.71 926.54
Standard Deviation 95.09 119.32 102.69 127.24 88.15 107.94 112.70 137.51 93.54 118.72 108.52 134.96
Intensity Change Log 2 — 0.261884 0.340762 0.371605 — 0.202905 0.393805 0.519054 — 0.233462 0.366838 0.445297

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000062 0.000201 0.000195 0.000961 0.000132 0.000188 0.000301 0.001262 0.000095 0.000194 0.000254 0.001126
Bud Neck 0.000774 0.004353 0.011788 0.013413 0.001455 0.004400 0.008728 0.018876 0.001097 0.004380 0.010103 0.016408
Bud Site 0.000225 0.001430 0.001333 0.008043 0.000560 0.001507 0.001248 0.008924 0.000384 0.001474 0.001286 0.008526
Cell Periphery 0.000090 0.000142 0.000066 0.000138 0.000181 0.000153 0.000065 0.000198 0.000133 0.000148 0.000065 0.000171
Cytoplasm 0.104788* 0.009662 0.003251 0.026096 0.053526 0.013126 0.005432 0.020278 0.080520 0.011650 0.004452 0.022907
Cytoplasmic Foci 0.004080 0.002893 0.000026 0.003551 0.005881 0.001989 0.000060 0.001475 0.004933 0.002374 0.000045 0.002413
Eisosomes 0.000011 0.000014 0.000014 0.000024 0.000017 0.000016 0.000012 0.000023 0.000014 0.000015 0.000013 0.000024
Endoplasmic Reticulum 0.000971 0.003927 0.004063 0.006024 0.001921 0.003532 0.003645 0.006707 0.001421 0.003701 0.003832 0.006399
Endosome 0.000232 0.000590 0.000130 0.004305 0.000797 0.000913 0.000237 0.004274 0.000500 0.000776 0.000189 0.004288
Golgi 0.000043 0.001180 0.000017 0.005557 0.000132 0.000216 0.000037 0.002570 0.000085 0.000627 0.000028 0.003920
Lipid Particles 0.000571 0.000408 0.000029 0.000332 0.001144 0.001455 0.000050 0.000325 0.000842 0.001009 0.000040 0.000328
Mitochondria 0.000303 0.001335 0.000730 0.003793 0.001464 0.002522 0.000800 0.005978 0.000853 0.002017 0.000769 0.004991
Mitotic Spindle 0.000064 0.001877 0.000138 0.029230 0.000994 0.003555 0.001790 0.011068 0.000504 0.002840 0.001048 0.019275
None 0.016760 0.004250 0.000942 0.007206 0.021992 0.005556 0.002048 0.009203 0.019237 0.004999 0.001551 0.008301
Nuclear Periphery 0.000434 0.000834 0.000957 0.002732 0.000565 0.000920 0.000556 0.001796 0.000496 0.000884 0.000736 0.002219
Nuclear Periphery Foci 0.000243 0.000188 0.000024 0.001704 0.000855 0.000555 0.000049 0.000611 0.000533 0.000398 0.000038 0.001105
Nucleolus 0.001767 0.002719 0.000444 0.002290 0.005981 0.004669 0.000869 0.002062 0.003762 0.003838 0.000678 0.002165
Nucleus 0.867214* 0.961091* 0.974856* 0.870809* 0.900525* 0.950613* 0.972505* 0.889122* 0.882984* 0.955078* 0.973562* 0.880847*
Peroxisomes 0.000173 0.000624 0.000022 0.000710 0.000304 0.001464 0.000174 0.000390 0.000235 0.001106 0.000106 0.000535
Vacuole 0.001130 0.002017 0.000931 0.010305 0.001315 0.002033 0.001216 0.010682 0.001218 0.002026 0.001088 0.010512
Vacuole Periphery 0.000063 0.000263 0.000044 0.002776 0.000259 0.000620 0.000178 0.004175 0.000156 0.000468 0.000118 0.003543

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.09 -9.20 -9.73 -8.02 -7.85 -1.58 -6.16 -2.44 -2.29 -1.87 -4.36 -9.89 -4.38 -3.89 -3.41
Bud Neck -4.07 -14.78 -14.82 -8.36 -1.60 -3.58 -10.02 -12.25 -9.06 -5.64 -5.46 -17.17 -17.90 -12.12 -5.63
Bud Site -2.65 -2.32 -6.04 -4.22 -3.74 -2.05 -2.28 -5.91 -4.62 -4.91 -3.36 -3.30 -8.39 -6.23 -6.16
Cell Periphery -1.19 3.25 -1.42 0.81 -3.83 0.62 3.16 0.77 0.09 -4.89 -0.51 3.82 0.14 0.70 -6.09
Cytoplasm 21.00 23.76 17.23 -5.27 -9.67 11.50 14.91 9.08 -3.22 -8.24 23.37 27.57 19.25 -5.85 -12.60
Cytoplasmic Foci 1.35 8.10 3.80 1.22 -3.62 4.27 6.97 5.72 2.25 -3.44 4.14 10.30 7.02 2.20 -4.93
Eisosomes -1.84 -3.66 -13.10 -9.56 -10.82 0.69 3.31 -7.42 -7.56 -12.14 -1.00 -0.04 -14.80 -12.04 -16.16
Endoplasmic Reticulum -11.36 -18.86 -21.24 -9.44 -9.59 -6.46 -11.56 -15.99 -10.12 -9.99 -12.74 -21.83 -25.80 -13.84 -13.77
Endosome -2.24 3.80 -4.29 -3.27 -4.53 -0.26 2.74 -3.10 -2.88 -4.32 -1.37 3.10 -5.00 -4.11 -6.04
Golgi -1.80 5.12 -2.82 -1.08 -2.85 -1.34 3.93 -2.90 -2.71 -3.12 -2.00 4.67 -4.00 -2.36 -4.14
Lipid Particles 1.52 7.43 2.43 0.30 -6.59 -0.57 4.98 3.62 2.40 -8.42 -0.50 7.24 4.32 2.41 -10.46
Mitochondria -3.02 -8.86 -7.71 -3.17 -5.81 -1.19 1.20 -3.32 -1.71 -4.88 -2.30 -0.10 -5.92 -2.63 -6.66
Mitotic Spindle -1.76 -1.93 -7.39 -6.54 -7.35 -1.96 -1.29 -6.06 -4.40 -5.39 -2.73 -1.62 -9.60 -7.76 -9.07
None 7.84 11.71 7.57 -1.07 -6.24 8.31 10.84 7.07 -1.54 -5.48 11.35 15.70 10.01 -1.83 -7.89
Nuclear Periphery -4.16 -7.25 -6.83 -5.66 -4.88 -2.10 -1.18 -10.08 -6.17 -9.89 -3.73 -6.69 -10.91 -8.12 -8.90
Nuclear Periphery Foci 0.76 3.87 -1.97 -2.14 -2.59 1.04 2.89 0.96 -0.28 -4.91 0.95 3.63 -0.94 -1.85 -4.13
Nucleolus -1.50 8.68 -1.62 0.57 -5.82 1.37 7.35 5.38 3.63 -5.95 -0.12 8.90 4.10 3.29 -8.29
Nucleus -14.66 -19.71 -2.12 11.07 14.71 -7.83 -12.90 1.54 9.43 14.66 -15.73 -23.04 -0.67 14.28 20.72
Peroxisomes -1.88 3.84 -1.35 0.70 -2.28 -2.43 1.40 0.47 2.50 -0.87 -2.97 2.29 -1.00 2.55 -2.27
Vacuole -2.65 -0.10 -9.98 -8.79 -9.97 -1.65 -0.12 -9.72 -8.92 -9.61 -3.01 -0.25 -13.88 -12.49 -13.75
Vacuole Periphery -2.50 2.38 -3.14 -2.52 -3.18 -1.20 0.72 -3.15 -2.44 -3.29 -1.80 0.64 -4.18 -3.17 -4.27
Standard name
Human Ortholog
Description RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16
Localization
Cell Percentages nucleus (83%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp2

Prp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available