Standard name | |
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Human Ortholog | |
Description | RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 2 | 5 | 7 | 10 | 8 | – | 13 | 9 | 10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 22 | 20 | 17 | – | 27 | 25 | 28 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 7 | 5 | 7 | 29 | 40 | 45 | 52 | – | 34 | 34 | 47 | 19 | 57 | 89 | 10 | 9 | 4 | 1 | 2 | 1 | 0 | 3 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 2 | 1 | 5 | 3 | 5 | 0 | 0 | 0 | 1 | 2 | 4 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 3 | 5 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 1 | 2 |
Mitochondria | 8 | 15 | 63 | 96 | 171 | 403 | 524 | 481 | – | 506 | 399 | 537 | 13 | 2 | 2 | 12 | 9 | 11 | 1 | 1 | 0 | 0 | 7 | 10 |
Nucleus | 247 | 117 | 165 | 258 | 290 | 404 | 470 | 469 | – | 428 | 317 | 423 | 266 | 307 | 342 | 184 | 146 | 176 | 241 | 121 | 173 | 99 | 210 | 213 |
Nuclear Periphery | 0 | 0 | 1 | 5 | 4 | 2 | 4 | 4 | – | 3 | 5 | 9 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 3 | 7 | 5 | – | 4 | 5 | 7 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 6 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 2 | 0 | 4 | 3 | – | 9 | 0 | 5 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 3 | 0 | 3 | 4 |
Vac/Vac Membrane | 2 | 9 | 2 | 2 | 20 | 7 | 15 | 8 | – | 13 | 11 | 13 | 4 | 3 | 12 | 14 | 10 | 10 | 7 | 7 | 9 | 14 | 62 | 71 |
Unique Cell Count | 263 | 133 | 189 | 287 | 399 | 592 | 710 | 723 | 691 | 520 | 698 | 294 | 367 | 442 | 205 | 164 | 192 | 261 | 139 | 194 | 127 | 311 | 340 | |
Labelled Cell Count | 267 | 148 | 238 | 372 | 526 | 889 | 1099 | 1049 | 1037 | 805 | 1079 | 307 | 372 | 448 | 231 | 181 | 209 | 261 | 139 | 194 | 127 | 311 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.3 | 4.0 | 3.7 | 3.8 | 3.5 | 3.0 | 3.3 | – | 3.0 | 3.0 | 2.9 | 5.9 | 6.2 | 6.2 | 6.9 | 7.0 | 7.2 | 6.6 | 6.9 | 7.1 |
Std Deviation (1e-4) | 0.8 | 1.2 | 0.8 | 1.1 | 1.5 | 1.0 | 0.9 | 1.2 | – | 0.9 | 1.2 | 0.8 | 1.1 | 1.7 | 1.7 | 1.1 | 1.6 | 1.3 | 1.8 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.08 | -0.2 | -0.41 | -0.29 | – | -0.43 | -0.4 | -0.45 | 0.56 | 0.64 | 0.64 | 0.79 | 0.81 | 0.85 | 0.71 | 0.79 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.2 | 2.1 | 2.0 | 2.3 | – | 1.3 | 2.0 | 2.1 | 1.9 | 4.6 | 5.7 | 1.2 | 1.4 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -8.5 | -11.4 | -12.4 | -6.9 | -6.5 | -6.8 |
Nucleus | 0.9 | -4.0 | -5.1 | -5.7 | -6.0 | – | -6.6 | -6.6 | -6.9 | 1.1 | -1.1 | -2.9 | 0.8 | 0.5 | 1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.6 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1414 | 1.0853 | 0.7037 | 0.8089 | 0.7645 | 0.961 | 2.1324 | 2.9193 | 2.3982 | 2.0687 | 1.7492 | 2.4698 | 0.5049 | 0.5934 | 0.0829 | 0.0523 | -0.1257 | 0.0501 |
Actin | 0.0195 | 0 | 0.0002 | 0.0024 | 0.001 | 0.0019 | 0.0061 | 0 | 0.0075 | 0 | 0.0001 | 0.0093 | 0.0026 | 0.0001 | 0.0103 | 0.0177 | 0 | 0 |
Bud | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0006 | 0.0002 | 0.0008 | 0 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0 | 0.0001 |
Bud Neck | 0.0013 | 0.0001 | 0.0001 | 0.0003 | 0.004 | 0.0005 | 0.001 | 0.0002 | 0.0018 | 0.0002 | 0.0188 | 0.0019 | 0.0001 | 0.0001 | 0.0014 | 0.007 | 0.0001 | 0.0024 |
Bud Periphery | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0019 | 0 | 0.0024 | 0.0001 | 0.0025 | 0 | 0.0006 | 0.0012 | 0 | 0.0002 | 0.0007 | 0.0012 | 0 | 0.0001 |
Bud Site | 0.0015 | 0.0001 | 0 | 0.0004 | 0.0139 | 0 | 0.0009 | 0.0001 | 0.0021 | 0 | 0.0352 | 0.0008 | 0.0006 | 0.0001 | 0.0014 | 0.0042 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0008 | 0 | 0.0006 | 0.0002 | 0 | 0 | 0.0003 | 0.0006 | 0 | 0 |
Cytoplasm | 0.0044 | 0.0009 | 0.0011 | 0.0005 | 0.0022 | 0.0001 | 0.0182 | 0.0192 | 0.0034 | 0.0014 | 0.0043 | 0.0014 | 0.0013 | 0.0004 | 0.0017 | 0.0008 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0069 | 0 | 0.0009 | 0.0012 | 0.0012 | 0.0002 | 0.0313 | 0.0003 | 0.0043 | 0.0001 | 0.0004 | 0.0013 | 0.1126 | 0 | 0.0051 | 0.0274 | 0 | 0 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.0032 | 0 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0015 | 0.0004 | 0.0061 | 0 | 0.0001 | 0.0062 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0 | 0 |
Endosome | 0.0122 | 0.0001 | 0.0007 | 0.0138 | 0.0012 | 0.0003 | 0.0054 | 0.0003 | 0.0087 | 0 | 0.0003 | 0.0076 | 0.0083 | 0.0002 | 0.0093 | 0.0359 | 0 | 0 |
Golgi | 0.0039 | 0 | 0.0003 | 0.0029 | 0.0006 | 0.0001 | 0.0017 | 0 | 0.0019 | 0 | 0.0001 | 0.0076 | 0.004 | 0 | 0.0097 | 0.0328 | 0 | 0 |
Lipid Particles | 0.0118 | 0 | 0.0055 | 0.0051 | 0.0007 | 0.0003 | 0.0048 | 0.0001 | 0.0018 | 0 | 0 | 0.0016 | 0.0229 | 0 | 0.0076 | 0.0452 | 0 | 0 |
Mitochondria | 0.0017 | 0.0001 | 0.0012 | 0.0012 | 0.002 | 0.0002 | 0.0097 | 0.0003 | 0.0069 | 0.0002 | 0.0003 | 0.0111 | 0.0004 | 0.0012 | 0.0402 | 0.1132 | 0 | 0.0001 |
None | 0.0084 | 0.0022 | 0.0031 | 0.0005 | 0.0032 | 0.0002 | 0.0109 | 0.0291 | 0.0067 | 0.0009 | 0.0001 | 0.0007 | 0.0016 | 0.0001 | 0.0282 | 0.0003 | 0 | 0 |
Nuclear Periphery | 0.0211 | 0.004 | 0.0094 | 0.0082 | 0.0032 | 0.0078 | 0.006 | 0.0034 | 0.0032 | 0.0053 | 0.0014 | 0.0054 | 0.0017 | 0.0175 | 0.0035 | 0.0013 | 0.0001 | 0.0002 |
Nucleolus | 0.0065 | 0.0098 | 0.005 | 0.0062 | 0.0171 | 0.0152 | 0.0055 | 0.0299 | 0.0033 | 0.0041 | 0.011 | 0.0116 | 0.006 | 0.0085 | 0.0035 | 0.0047 | 0.0049 | 0.0274 |
Nucleus | 0.857 | 0.9749 | 0.9645 | 0.9469 | 0.9159 | 0.9629 | 0.8565 | 0.9094 | 0.9319 | 0.9465 | 0.9233 | 0.9113 | 0.7631 | 0.9682 | 0.8691 | 0.6654 | 0.9947 | 0.9688 |
Peroxisomes | 0.011 | 0 | 0.0024 | 0.0006 | 0.0011 | 0.0039 | 0.0141 | 0 | 0.0012 | 0 | 0.0001 | 0.0007 | 0.0412 | 0 | 0.0034 | 0.0324 | 0 | 0 |
Punctate Nuclear | 0.027 | 0.0077 | 0.005 | 0.0067 | 0.0277 | 0.0061 | 0.0162 | 0.0059 | 0.0015 | 0.0411 | 0.001 | 0.0159 | 0.033 | 0.0004 | 0.0009 | 0.0012 | 0 | 0.0001 |
Vacuole | 0.001 | 0.0001 | 0.0002 | 0.0008 | 0.0015 | 0.0001 | 0.0047 | 0.0009 | 0.0024 | 0.0001 | 0.0017 | 0.0023 | 0.0001 | 0.0009 | 0.0015 | 0.0059 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0007 | 0 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0024 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0016 | 0.0008 | 0.0014 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2107 | 8.2479 | 13.6316 | 12.6975 | 10.6032 | 7.1256 | 13.5516 | 13.3039 | 11.552 | 14.4705 |
Translational Efficiency | 0.9154 | 1.3371 | 0.6356 | 0.718 | 0.9281 | 1.4006 | 0.8271 | 0.7221 | 0.774 | 0.6572 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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