Standard name | |
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Description | RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 2 | 5 | 7 | 10 | 8 | – | 13 | 9 | 10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 22 | 20 | 17 | – | 27 | 25 | 28 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 7 | 5 | 7 | 29 | 40 | 45 | 52 | – | 34 | 34 | 47 | 19 | 57 | 89 | 10 | 9 | 4 | 1 | 2 | 1 | 0 | 3 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 2 | 1 | 5 | 3 | 5 | 0 | 0 | 0 | 1 | 2 | 4 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 3 | 5 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 1 | 2 |
Mitochondria | 8 | 15 | 63 | 96 | 171 | 403 | 524 | 481 | – | 506 | 399 | 537 | 13 | 2 | 2 | 12 | 9 | 11 | 1 | 1 | 0 | 0 | 7 | 10 |
Nucleus | 247 | 117 | 165 | 258 | 290 | 404 | 470 | 469 | – | 428 | 317 | 423 | 266 | 307 | 342 | 184 | 146 | 176 | 241 | 121 | 173 | 99 | 210 | 213 |
Nuclear Periphery | 0 | 0 | 1 | 5 | 4 | 2 | 4 | 4 | – | 3 | 5 | 9 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 3 | 7 | 5 | – | 4 | 5 | 7 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 6 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 2 | 0 | 4 | 3 | – | 9 | 0 | 5 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 3 | 0 | 3 | 4 |
Vac/Vac Membrane | 2 | 9 | 2 | 2 | 20 | 7 | 15 | 8 | – | 13 | 11 | 13 | 4 | 3 | 12 | 14 | 10 | 10 | 7 | 7 | 9 | 14 | 62 | 71 |
Unique Cell Count | 263 | 133 | 189 | 287 | 399 | 592 | 710 | 723 | 691 | 520 | 698 | 294 | 367 | 442 | 205 | 164 | 192 | 261 | 139 | 194 | 127 | 311 | 340 | |
Labelled Cell Count | 267 | 148 | 238 | 372 | 526 | 889 | 1099 | 1049 | 1037 | 805 | 1079 | 307 | 372 | 448 | 231 | 181 | 209 | 261 | 139 | 194 | 127 | 311 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.3 | 4.0 | 3.7 | 3.8 | 3.5 | 3.0 | 3.3 | – | 3.0 | 3.0 | 2.9 | 5.9 | 6.2 | 6.2 | 6.9 | 7.0 | 7.2 | 6.6 | 6.9 | 7.1 |
Std Deviation (1e-4) | 0.8 | 1.2 | 0.8 | 1.1 | 1.5 | 1.0 | 0.9 | 1.2 | – | 0.9 | 1.2 | 0.8 | 1.1 | 1.7 | 1.7 | 1.1 | 1.6 | 1.3 | 1.8 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.08 | -0.2 | -0.41 | -0.29 | – | -0.43 | -0.4 | -0.45 | 0.56 | 0.64 | 0.64 | 0.79 | 0.81 | 0.85 | 0.71 | 0.79 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.2 | 2.1 | 2.0 | 2.3 | – | 1.3 | 2.0 | 2.1 | 1.9 | 4.6 | 5.7 | 1.2 | 1.4 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -8.5 | -11.4 | -12.4 | -6.9 | -6.5 | -6.8 |
Nucleus | 0.9 | -4.0 | -5.1 | -5.7 | -6.0 | – | -6.6 | -6.6 | -6.9 | 1.1 | -1.1 | -2.9 | 0.8 | 0.5 | 1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.6 | 2.3 |