Standard name
Description Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.95 0.85 0.77 0.79 0.76 0.73 0.62 0.64 0.98 0.99 0.98 0.93 0.9 0.88 0.95 0.93 0.89 0.95 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.23 0.16 0.23 0.21 0.41 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.05 0.07 0.09 0.07 0.09 0.05 0.13 0.1 0.19 0 0 0 0.09 0.13 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 2 3 8 10 1 0 1 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1 2 2 3 0 1 0 0 0 2 1 3 8 5 8 0 0 0 0 0 0
Cytoplasm 191 204 140 167 312 221 231 282 185 201 183 173 382 576 544 136 144 128 179 191 143 175 382 459
Endoplasmic Reticulum 1 2 0 0 1 3 3 0 4 0 1 0 1 0 2 2 2 3 0 0 1 0 1 1
Endosome 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 2 3 0 0 0 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 0
Mitochondria 3 2 0 2 3 28 68 57 56 59 121 78 0 0 0 0 4 2 0 3 2 0 1 0
Nucleus 0 1 1 5 2 4 12 10 13 9 17 16 3 5 2 1 0 3 0 5 2 1 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 7 8 22 24 22 33 12 37 31 51 10 12 18 13 21 10 0 1 2 1 1 3
Unique Cell Count 193 206 144 172 327 259 299 359 244 277 296 269 389 582 555 146 160 145 189 206 160 185 395 477
Labelled Cell Count 195 209 148 183 342 282 342 387 275 316 364 319 398 595 569 162 178 158 189 206 160 185 395 477


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 8.7 7.6 7.3 7.3 6.1 5.6 6.1 5.3 5.8 5.0 5.4 7.6 8.0 8.0 12.1 11.9 11.9 7.8 8.5 8.9
Std Deviation (1e-4) 0.7 1.1 1.0 0.8 1.7 1.3 1.2 1.2 1.4 1.5 1.1 0.8 0.9 0.9 0.9 2.3 1.9 2.2 1.2 1.3 1.4
Intensity Change (Log2) -0.05 -0.06 -0.31 -0.44 -0.33 -0.52 -0.39 -0.6 -0.49 0.0 0.08 0.08 0.67 0.64 0.64 0.03 0.16 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.9 -3.7 -5.3 -5.1 -5.5 -6.1 -7.9 -7.4 0.7 1.6 0.6 -1.6 -2.5 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 6.2 5.1 6.2 6.0 9.0 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 2.4 0 2.5 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 0.8 1.6 1.0 1.6 0 2.7 2.0 3.9 0 0 -0.9 1.4 2.5 0.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available