Standard name
Human Ortholog
Description MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.96 0.95 0.95 0.9 0.89 0.83 0.69 0.75 0.67 0.59 1.0 0.98 0.98 0.96 0.94 0.92 0.9 0.9 0.83 0.9 0.91 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.09 0.2 0.09 0.27 0.32 0.32 0.51 0.48 0.6 0.64 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 5
Bud 1 0 10 2 6 5 5 9 6 9 7 12 0 1 4 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 1 5 3 10 5 8 8 5 6 0 3 2 14 20 29 0 0 0 0 0 0
Cytoplasm 146 117 177 124 293 230 390 334 204 249 142 199 67 155 160 392 296 346 150 127 153 93 179 204
Endoplasmic Reticulum 1 1 0 0 2 1 0 3 4 1 0 4 0 1 1 17 9 16 1 1 3 1 5 6
Endosome 2 0 0 0 3 0 1 3 0 1 0 0 0 0 0 5 3 2 3 0 3 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 5 3 0 0 1 0 2
Mitochondria 0 7 16 26 27 69 139 128 150 161 127 217 0 3 5 5 5 7 1 3 11 1 3 4
Nucleus 0 0 2 4 0 1 2 5 1 1 1 1 0 0 0 1 2 2 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 1 2 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 1 0 0 0
Vac/Vac Membrane 4 0 1 1 7 0 4 10 1 12 3 10 0 1 0 12 10 16 2 2 2 1 3 3
Unique Cell Count 154 122 185 130 307 256 437 401 295 332 211 340 67 158 163 408 314 376 168 141 184 104 197 233
Labelled Cell Count 157 126 207 158 343 310 552 501 376 445 287 452 67 164 173 450 348 423 168 141 184 104 197 233


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 4.3 4.2 4.5 4.7 4.2 4.2 4.4 3.6 3.8 3.6 3.6 6.0 6.4 5.5 7.2 7.8 8.0 6.2 6.4 6.9
Std Deviation (1e-4) 1.0 0.6 1.1 1.7 1.2 1.0 1.4 2.1 1.0 1.2 1.1 1.3 0.9 1.2 1.3 1.3 1.7 1.8 1.1 1.0 1.2
Intensity Change (Log2) – – – 0.09 0.17 0.0 -0.0 0.06 -0.22 -0.14 -0.21 -0.23 0.53 0.61 0.41 0.79 0.91 0.94 0.57 0.6 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7025 2.8333 2.1936 2.1429 1.5871 2.4995 1.9881 2.7595 2.5902 2.6341 2.5131 2.5981 2.6841 4.3222 3.3921 3.0279 2.9652 3.3171
Actin 0.0375 0.0003 0.0124 0.0212 0.0321 0.0002 0.0226 0.0005 0.0098 0.0002 0.0154 0.0056 0.113 0.0703 0.0034 0.0022 0.0001 0.0002
Bud 0.0005 0.0003 0.0004 0.0065 0.0003 0.0001 0.0057 0.0139 0.0069 0.0006 0.0028 0.0004 0.0016 0.0005 0.0004 0.0003 0.0001 0
Bud Neck 0.0094 0.0003 0.0034 0.0013 0.0107 0.0009 0.003 0.0002 0.001 0.0003 0.0012 0.0023 0.0041 0.0004 0.0006 0.0004 0.0002 0.0003
Bud Periphery 0.0008 0.0002 0.0005 0.0127 0.0003 0.0001 0.0039 0.0024 0.0065 0.0003 0.0026 0.0007 0.0014 0.0003 0.0002 0.0003 0 0
Bud Site 0.0024 0.0018 0.0029 0.0037 0.0004 0.0002 0.0186 0.0066 0.0076 0.0002 0.0023 0.0002 0.0436 0.0091 0.0071 0.0002 0.0001 0
Cell Periphery 0.0006 0.0011 0.0007 0.0004 0.0002 0.0002 0.0009 0.0003 0.0004 0.0001 0.0005 0.0001 0.0015 0.0002 0.0005 0.0002 0 0.0001
Cytoplasm 0.6496 0.9103 0.7988 0.8493 0.8518 0.8823 0.5187 0.7856 0.715 0.7675 0.594 0.7736 0.4555 0.4916 0.8128 0.7547 0.9856 0.9127
Cytoplasmic Foci 0.0215 0.0074 0.011 0.0138 0.0107 0.0087 0.0409 0.0184 0.0142 0.0153 0.042 0.0175 0.027 0.0131 0.0269 0.0084 0.0032 0.0063
Eisosomes 0.0004 0 0.0002 0.0002 0.0003 0 0.0005 0 0.0001 0 0.0001 0 0.0007 0.0002 0 0 0 0
Endoplasmic Reticulum 0.0195 0.0081 0.0065 0.006 0.0047 0.0068 0.0074 0.0024 0.0022 0.0024 0.0028 0.0021 0.028 0.0052 0.0065 0.0166 0.0015 0.0068
Endosome 0.0212 0.005 0.0091 0.0208 0.0164 0.0077 0.0312 0.0085 0.0107 0.0071 0.0195 0.0086 0.0078 0.008 0.0253 0.0113 0.0029 0.0048
Golgi 0.0051 0.0006 0.003 0.0051 0.006 0.0003 0.0126 0.0009 0.0016 0.0003 0.0074 0.0007 0.0079 0.0021 0.007 0.0006 0.0002 0.0003
Lipid Particles 0.0175 0.0006 0.0086 0.0184 0.0081 0.0008 0.0129 0.0002 0.0018 0.0002 0.01 0.0003 0.0078 0.0009 0.003 0.0003 0 0.0001
Mitochondria 0.0032 0.0003 0.0121 0.0019 0.002 0.0003 0.0084 0.0003 0.0091 0.0004 0.0282 0.0003 0.0071 0.0005 0.0004 0.0002 0.0001 0.0001
None 0.1491 0.0585 0.104 0.0115 0.0435 0.0848 0.2321 0.1559 0.1926 0.1978 0.2186 0.1836 0.2408 0.3857 0.0957 0.1974 0.0045 0.0658
Nuclear Periphery 0.0134 0.0007 0.0012 0.0038 0.0038 0.0015 0.0111 0.0004 0.0013 0.0006 0.0041 0.0005 0.0014 0.0006 0.0011 0.0025 0.0003 0.0004
Nucleolus 0.0014 0.0001 0.001 0.0002 0.0001 0.0001 0.0018 0.0001 0.0003 0.0004 0.0006 0 0.0003 0.0001 0.0001 0 0 0
Nucleus 0.0135 0.001 0.0051 0.002 0.0011 0.0011 0.0095 0.001 0.0031 0.0019 0.0103 0.001 0.0009 0.0008 0.0009 0.0009 0.0004 0.0005
Peroxisomes 0.0081 0.0001 0.0123 0.0099 0.0029 0.0001 0.0363 0.0003 0.01 0.0001 0.0247 0.0003 0.0454 0.0057 0.0012 0.0001 0 0
Punctate Nuclear 0.0161 0.0004 0.0009 0.0038 0.0024 0.001 0.0127 0.0005 0.0008 0.002 0.0057 0.0007 0.0009 0.0009 0.0007 0.0004 0.0001 0.0001
Vacuole 0.0071 0.0028 0.0052 0.0063 0.0019 0.0023 0.0057 0.0015 0.0042 0.0021 0.0062 0.0012 0.0031 0.0035 0.0054 0.0024 0.0006 0.0012
Vacuole Periphery 0.002 0.0002 0.0006 0.0011 0.0006 0.0003 0.0035 0.0002 0.0007 0.0004 0.0007 0.0002 0.0003 0.0004 0.0007 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.3425 15.8153 15.2872 21.3111 22.4854 20.0085 23.1972 24.6387 24.6475 24.7521
Translational Efficiency 0.5144 0.5585 0.4966 0.3717 0.3596 0.4431 0.425 0.3196 0.3342 0.3582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
204 1852 138 1077 2261 1949 850 1708 2465 3801 988 2785

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 815.48 842.98 1180.79 876.15 766.89 771.25 953.02 898.96 770.91 806.20 984.83 890.14
Standard Deviation 128.85 107.42 136.72 121.64 90.32 109.60 110.22 126.84 95.06 114.31 138.91 125.35
Intensity Change Log 2 — 0.047849 0.534031 0.103528 — 0.008179 0.313487 0.229237 — 0.028759 0.431345 0.165821

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000395 0.000270 0.000396 0.000157 0.000166 0.000624 0.000103 0.000091 0.000185 0.000452 0.000144 0.000116
Bud Neck 0.017663 0.009051 0.002404 0.005189 0.010013 0.016565 0.001614 0.000725 0.010646 0.012904 0.001724 0.002451
Bud Site 0.020442 0.013679 0.007831 0.014691 0.009157 0.023107 0.009204 0.003840 0.010091 0.018513 0.009013 0.008036
Cell Periphery 0.000177 0.000079 0.000237 0.000187 0.000093 0.000094 0.000094 0.000044 0.000100 0.000087 0.000114 0.000100
Cytoplasm 0.523847* 0.603656* 0.424167* 0.587647* 0.592771* 0.539517* 0.693594* 0.827092* 0.587067* 0.570768* 0.655962* 0.734495*
Cytoplasmic Foci 0.220894* 0.220478* 0.049170 0.048519 0.200956* 0.215597* 0.042995 0.044202 0.202606* 0.217975* 0.043858 0.045872
Eisosomes 0.000165 0.000051 0.000077 0.000054 0.000101 0.000072 0.000035 0.000010 0.000106 0.000062 0.000041 0.000027
Endoplasmic Reticulum 0.002542 0.001134 0.003812 0.001017 0.000996 0.000784 0.001141 0.000347 0.001124 0.000955 0.001514 0.000606
Endosome 0.016755 0.021004 0.015352 0.008968 0.015990 0.028609 0.005442 0.002403 0.016053 0.024904 0.006826 0.004942
Golgi 0.002172 0.005180 0.000222 0.002455 0.005002 0.006860 0.000163 0.000296 0.004768 0.006042 0.000171 0.001131
Lipid Particles 0.005652 0.002602 0.004517 0.001567 0.003319 0.003590 0.001188 0.000370 0.003512 0.003109 0.001653 0.000833
Mitochondria 0.001317 0.002634 0.000115 0.001302 0.002719 0.005648 0.000116 0.000058 0.002603 0.004179 0.000116 0.000539
Mitotic Spindle 0.001898 0.001934 0.002730 0.012709 0.001056 0.008205 0.003667 0.004033 0.001126 0.005150 0.003536 0.007388
None 0.000639 0.002058 0.003841 0.004660 0.001944 0.001293 0.002854 0.003967 0.001836 0.001666 0.002992 0.004235
Nuclear Periphery 0.000376 0.000146 0.001230 0.000590 0.000129 0.000202 0.000612 0.000142 0.000150 0.000175 0.000698 0.000315
Nuclear Periphery Foci 0.003559 0.000648 0.012392 0.001499 0.000411 0.000439 0.002521 0.001815 0.000672 0.000541 0.003900 0.001692
Nucleolus 0.000507 0.000544 0.000250 0.000670 0.000669 0.000874 0.000242 0.000121 0.000655 0.000713 0.000243 0.000333
Nucleus 0.036210 0.028237 0.103333 0.137075* 0.045984 0.025980 0.095804 0.054938 0.045175 0.027080 0.096856 0.086701
Peroxisomes 0.003284 0.003110 0.000415 0.001340 0.002462 0.005559 0.000418 0.000311 0.002530 0.004365 0.000418 0.000709
Vacuole 0.140081 0.080801 0.367098* 0.168034 0.103977 0.113208 0.137927 0.055020 0.106965 0.097418 0.169937 0.098724
Vacuole Periphery 0.001426 0.002704 0.000410 0.001672 0.002083 0.003171 0.000263 0.000174 0.002029 0.002944 0.000283 0.000754

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.86 -0.78 1.87 0.88 6.23 -3.03 2.47 3.58 3.48 1.80 -2.83 0.50 2.79 3.47 3.27
Bud Neck 1.78 3.07 2.82 4.83 -1.15 -5.33 13.04 15.72 14.71 2.86 -2.45 12.56 11.91 15.27 -0.80
Bud Site 1.77 3.05 1.86 0.52 -1.73 -8.47 1.00 6.83 12.07 1.97 -7.66 1.58 2.77 9.31 0.20
Cell Periphery 5.29 -1.68 1.51 -3.12 2.87 -0.10 1.02 12.40 6.67 8.08 2.44 -1.03 2.46 0.82 2.88
Cytoplasm -4.43 5.30 -0.85 6.50 -7.10 6.42 -3.38 -27.63 -30.80 -15.76 2.34 -0.37 -14.44 -17.52 -9.73
Cytoplasmic Foci -0.33 13.76 14.27 35.94 -0.17 -2.48 36.84 37.83 35.77 -1.47 -3.22 39.39 41.17 50.81 -1.31
Eisosomes 4.54 3.40 4.67 1.03 3.99 3.66 8.64 12.71 17.01 14.43 6.24 8.91 11.79 15.67 8.69
Endoplasmic Reticulum 1.51 -1.60 1.55 0.06 5.78 1.13 -1.36 4.86 2.80 5.69 0.93 -2.72 3.23 2.14 6.43
Endosome -2.03 2.29 5.30 10.47 3.29 -8.37 10.59 16.54 20.70 3.23 -7.69 10.55 14.75 22.98 2.32
Golgi -5.13 3.74 0.83 5.88 -2.38 -3.12 17.56 16.89 12.52 -1.83 -3.12 17.89 12.21 14.07 -2.79
Lipid Particles 1.61 0.98 2.28 4.67 4.53 -0.70 8.53 12.92 9.87 6.11 1.21 6.98 10.44 11.28 5.30
Mitochondria -2.09 3.02 0.85 2.88 -2.29 -3.16 5.36 5.47 7.14 4.18 -2.39 5.55 4.82 7.72 -2.06
Mitotic Spindle 0.11 -0.58 -3.43 -3.70 -2.98 -4.43 -1.36 -2.14 2.28 -0.49 -4.38 -1.50 -4.38 -0.86 -2.20
None -2.72 -1.98 -4.49 -2.10 -1.05 1.81 -1.74 -3.30 -4.81 -1.99 0.37 -2.10 -4.17 -4.45 -2.37
Nuclear Periphery 1.72 -6.10 -2.80 -14.34 5.54 -2.14 -10.29 -4.25 -0.15 9.05 -0.90 -12.48 -11.62 -9.90 7.49
Nuclear Periphery Foci 1.22 -3.08 0.80 -6.23 8.04 -0.31 -11.41 -8.95 -8.28 2.94 0.65 -9.84 -4.89 -10.03 7.47
Nucleolus -0.16 0.96 -0.02 0.31 -2.31 -1.29 4.24 5.26 5.76 3.12 -0.53 4.36 3.55 5.04 -1.07
Nucleus 2.03 -5.84 -14.42 -21.07 0.00 12.10 -14.71 -7.75 -17.37 10.17 12.55 -15.60 -17.08 -26.34 5.36
Peroxisomes 0.11 4.50 3.46 7.29 -2.23 -6.28 7.37 7.66 12.60 0.27 -5.32 8.14 7.29 15.13 -1.44
Vacuole 5.46 -9.94 -6.59 -20.21 6.72 -2.32 -11.46 3.85 5.57 13.00 3.09 -14.62 -8.88 -11.69 8.75
Vacuole Periphery -3.07 3.49 0.76 3.62 -2.04 -3.65 9.77 10.54 12.73 0.65 -3.62 10.19 7.06 10.54 -1.89