Standard name
Human Ortholog
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.48 0.35 0.53 0.33 0.44 0.22 0.32 0.34 0.12 0.49 0.44 0.34 0.37 0.45 0.53 0.81 0.81 0.84 0.82 0.82 0.8
Bud 0.12 0.07 0.12 0 0 0.11 0.11 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.07 0.07 0.11 0.07 0 0 0 0 0.12 0.13 0.14 0.05 0.11 0.06 0 0 0 0 0 0
Cytoplasm 0.05 0.09 0.05 0.17 0 0 0 0 0.06 0 0.07 0.05 0.05 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.15 0.12 0.06 0.11 0 0 0.06 0 0.19 0.15 0.07 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.36 0.33 0.53 0.67 0.62 0.22 0.27 0.4 0 0.68 0.66 0.85 0.67 0.57 0.58 0 0.05 0.05 0 0.05 0.07
Mitochondria 0.65 0.32 0.12 0.17 0.27 0.64 0.72 0.54 0.62 0.28 0.05 0 0.18 0.11 0.17 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.07 0.06 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0 0.06 0 0.09 0.06 0 0 0 0 0 0 0 0 0.06 0 0 0.05
SpindlePole 0 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 37 48 23 6 32 8 24 23 2 60 95 78 37 51 56 104 153 51 88 157 185
Bud 9 10 5 0 2 4 8 1 1 3 14 4 2 3 3 4 2 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 1 0 1 3 0 0 0 0 1 0 0
Cell Periphery 2 10 3 2 5 1 3 1 0 15 29 31 5 13 6 0 2 0 0 1 2
Cytoplasm 4 12 2 3 3 1 2 1 1 5 15 12 5 11 12 1 2 0 0 1 0
Endoplasmic Reticulum 5 20 5 1 8 1 2 4 0 23 32 17 2 6 3 0 2 0 0 1 1
Endosome 2 0 1 0 0 0 0 0 2 0 2 0 0 2 0 3 2 1 3 0 0
Golgi 28 45 23 12 45 8 20 27 0 83 142 194 66 65 62 3 10 3 3 9 16
Mitochondria 50 43 5 3 20 23 53 37 10 34 11 8 18 12 18 3 4 0 1 2 5
Nucleus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 0 0 1
Nucleolus 3 3 0 0 0 2 5 4 2 2 1 3 0 2 0 0 0 0 2 2 2
Peroxisomes 1 1 1 1 2 2 3 6 1 2 2 3 1 0 1 3 2 3 4 8 10
SpindlePole 0 0 2 0 1 0 0 2 1 0 0 1 1 0 1 0 0 1 0 0 0
Vac/Vac Membrane 0 2 0 0 1 3 0 2 0 1 4 3 5 1 3 0 0 0 0 0 0
Unique Cell Count 77 136 43 18 73 36 74 68 16 122 216 229 99 114 106 130 189 61 108 192 233
Labelled Cell Count 141 195 71 28 120 54 121 111 20 229 349 356 143 166 165 130 189 61 108 192 233


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.4 35.9 27.5 23.4 24.6 20.3 22.7 25.2 17.4 27.0 29.0 30.0 35.4 38.1 36.3 28.5 31.8 35.0
Std Deviation (1e-4) 8.5 12.4 6.7 8.0 6.2 5.8 7.0 8.9 6.8 6.5 9.9 11.8 9.9 14.3 15.1 8.9 9.1 11.0
Intensity Change (Log2) -0.23 -0.16 -0.44 -0.28 -0.13 -0.66 -0.03 0.07 0.12 0.36 0.47 0.4 0.05 0.21 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 -1.0 -2.8 -2.2 -2.0 -2.8 -0.5 -1.1 -2.4 -1.8 -1.0 -0.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1.2 1.2 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 1.1 0.5 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.9 0.9 -2.8 -2.9 -1.4 -3.7 1.7 1.5 4.7 1.5 0.4 0.6
Mitochondria 0 2.0 4.8 6.3 4.5 0 2.2 0 0 1.0 0 0.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.6584 0.9138 0.8328 0.6671 0.5982 0.8865 0.7563 0.8975 0.844 0.4936 0.5904 0.7416 0.6294 0.8755 0.8052 0.9323 0.8395 0.7759
Bud 0.0008 0.0003 0.0005 0.0012 0.0011 0.0006 0.0026 0.0006 0.0004 0.002 0.0009 0.0005 0.0018 0.0001 0.0007 0.0001 0.0005 0.0002
Bud Neck 0.0019 0.0017 0.0086 0.0026 0.0024 0.0014 0.004 0.0023 0.0032 0.017 0.0022 0.0047 0.0025 0.0014 0.0017 0.0185 0.0022 0.0017
Bud Periphery 0.0008 0.0003 0.0005 0.0009 0.002 0.0005 0.0047 0.0006 0.0005 0.0034 0.0023 0.0004 0.002 0.0001 0.0006 0.0002 0.0009 0.0002
Bud Site 0.0057 0.0117 0.0078 0.009 0.0019 0.0009 0.0159 0.0282 0.0036 0.0068 0.002 0.0005 0.0058 0.0063 0.0105 0.0007 0.0023 0.0004
Cell Periphery 0.0002 0.0007 0.0004 0.0006 0.0004 0.0001 0.0015 0.0003 0.0008 0.0016 0.0005 0.0003 0.0015 0.0003 0.0003 0.0003 0.0002 0.0001
Cytoplasm 0.0185 0.0003 0.0024 0.0743 0.009 0.0018 0.0189 0 0.0003 0.0017 0.0003 0.0061 0.0135 0.0001 0.0035 0 0.0004 0.0003
Cytoplasmic Foci 0.0617 0.0011 0.0225 0.0253 0.0055 0.0041 0.0034 0.0002 0.0079 0.0752 0.0021 0.003 0.0372 0.0007 0.0171 0.0001 0.0042 0.0061
Eisosomes 0.075 0.0579 0.0471 0.0084 0.0577 0.0451 0.1188 0.067 0.1039 0.0092 0.1944 0.2187 0.0976 0.1053 0.0755 0.0448 0.0332 0.0985
Endoplasmic Reticulum 0.0067 0.0001 0.0015 0.0084 0.0036 0.0008 0.0123 0 0.0001 0.001 0.0024 0.001 0.0149 0.0001 0.0015 0 0.0011 0.0005
Endosome 0.0341 0.0012 0.0211 0.0541 0.1248 0.0111 0.0073 0 0.0005 0.1351 0.0169 0.0106 0.0525 0.0002 0.0075 0 0.0123 0.0141
Golgi 0.0136 0.0079 0.0189 0.0235 0.0087 0.0066 0.0107 0.0006 0.0032 0.0902 0.0578 0.0054 0.0302 0.0067 0.012 0.0008 0.0074 0.052
Lipid Particles 0.0248 0.0004 0.006 0.0246 0.0049 0.0011 0.0027 0.0001 0.0043 0.0841 0.0061 0.0002 0.0126 0.0005 0.0097 0.0002 0.0089 0.0122
Mitochondria 0.0058 0.0012 0.0172 0.0145 0.0399 0.0013 0.0243 0.0015 0.0206 0.0237 0.0827 0.0025 0.0254 0.0016 0.0375 0.0009 0.0309 0.0246
None 0.0167 0 0.0024 0.0017 0.0009 0.001 0.0008 0 0.0008 0.0002 0.0004 0.0004 0.009 0.0001 0.0018 0 0.0009 0.0004
Nuclear Periphery 0.0219 0 0.0006 0.022 0.0555 0.0181 0.0035 0 0.0001 0.0007 0.0224 0.0014 0.0161 0 0.0008 0 0.0265 0.0021
Nucleolus 0.0015 0 0.0005 0.0002 0.0015 0.0015 0.0001 0 0.0005 0.0054 0.0008 0 0.0029 0 0.0009 0 0.002 0.001
Nucleus 0.0158 0 0.0004 0.004 0.0421 0.0072 0.0006 0 0.0001 0.0028 0.0028 0.0006 0.0116 0 0.0006 0 0.0064 0.0009
Peroxisomes 0.0107 0.0012 0.0029 0.0068 0.0018 0.0012 0.009 0.0012 0.0032 0.0142 0.0035 0.001 0.0123 0.001 0.0088 0.001 0.004 0.0023
Punctate Nuclear 0.0217 0 0.0018 0.0006 0.0017 0.0067 0.0002 0 0.0002 0.0022 0.0011 0.0006 0.0087 0 0.0028 0 0.0011 0.0055
Vacuole 0.002 0.0001 0.0033 0.0285 0.0269 0.0016 0.0013 0 0.0009 0.0248 0.0032 0.0004 0.0095 0 0.0004 0 0.0048 0.0004
Vacuole Periphery 0.0017 0 0.0008 0.0214 0.0095 0.001 0.0009 0 0.0008 0.0049 0.0047 0.0003 0.0032 0 0.0005 0 0.0104 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.7423 82.4377 71.8419 95.2295 82.7225 80.3785 87.4718 87.3298 100.049 90.3026
Translational Efficiency 1.2932 1.1108 1.3547 1.2307 1.2789 1.3939 1.2468 1.4001 1.2492 1.1695

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
260 546 2071 1429 1301 1235 1548 1047 1561 1781 3619 2476

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1737.64 2017.97 2328.82 2464.76 1893.14 1955.08 2585.30 2464.39 1867.24 1974.36 2438.53 2464.60
Standard Deviation 368.83 448.71 529.58 642.63 448.10 531.75 672.71 711.39 439.73 508.56 608.41 672.57
Intensity Change Log 2 0.215776 0.422470 0.504318 0.046447 0.449551 0.380449 0.129967 0.436654 0.441059

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.810661* 0.832423* 0.869435* 0.830053* 0.844421* 0.846524* 0.777952* 0.811886* 0.838798* 0.842201* 0.830304* 0.822371*
Bud Neck 0.011330 0.005852 0.007671 0.007307 0.006952 0.008125 0.009241 0.008159 0.007681 0.007428 0.008343 0.007668
Bud Site 0.008325 0.005212 0.015591 0.034421 0.007493 0.007972 0.014104 0.029955 0.007631 0.007126 0.014955 0.032533
Cell Periphery 0.001293 0.000482 0.002332 0.006011 0.000879 0.001300 0.002715 0.002351 0.000948 0.001049 0.002496 0.004463
Cytoplasm 0.000083 0.000079 0.000170 0.001259 0.000203 0.000692 0.000460 0.001569 0.000183 0.000504 0.000294 0.001390
Cytoplasmic Foci 0.000765 0.001276 0.000352 0.002616 0.001010 0.002364 0.000826 0.005775 0.000969 0.002030 0.000555 0.003952
Eisosomes 0.129985* 0.118006* 0.096498 0.093179 0.113696* 0.068224 0.156606* 0.053882 0.116409* 0.083485 0.122209* 0.076562
Endoplasmic Reticulum 0.004689 0.002415 0.001340 0.002360 0.002297 0.008047 0.005334 0.008140 0.002695 0.006320 0.003048 0.004804
Endosome 0.003646 0.001710 0.000659 0.002239 0.001123 0.007100 0.003350 0.011152 0.001544 0.005448 0.001810 0.006008
Golgi 0.012722 0.012187 0.001017 0.004586 0.004549 0.014151 0.007231 0.020938 0.005910 0.013549 0.003675 0.011500
Lipid Particles 0.010660 0.011030 0.000550 0.001471 0.004276 0.021452 0.017398 0.026045 0.005339 0.018257 0.007757 0.011862
Mitochondria 0.004130 0.004442 0.003413 0.008271 0.009859 0.005624 0.002578 0.007760 0.008905 0.005262 0.003056 0.008055
Mitotic Spindle 0.000066 0.000023 0.000151 0.000259 0.000134 0.000963 0.000113 0.002933 0.000123 0.000675 0.000135 0.001390
None 0.000128 0.000127 0.000042 0.000097 0.000132 0.000194 0.000142 0.000115 0.000132 0.000173 0.000085 0.000105
Nuclear Periphery 0.000048 0.000114 0.000023 0.000060 0.000026 0.000063 0.000071 0.000181 0.000030 0.000079 0.000043 0.000111
Nuclear Periphery Foci 0.000053 0.000109 0.000008 0.000105 0.000012 0.000340 0.000043 0.000390 0.000019 0.000269 0.000023 0.000226
Nucleolus 0.000010 0.000010 0.000005 0.000033 0.000011 0.000028 0.000105 0.000350 0.000011 0.000022 0.000048 0.000167
Nucleus 0.000058 0.000030 0.000023 0.000068 0.000045 0.000077 0.000064 0.000318 0.000047 0.000063 0.000041 0.000174
Peroxisomes 0.001018 0.003481 0.000471 0.001635 0.002624 0.005366 0.000613 0.003961 0.002357 0.004788 0.000532 0.002619
Vacuole 0.000190 0.000298 0.000202 0.002210 0.000160 0.000897 0.000852 0.002981 0.000165 0.000713 0.000480 0.002536
Vacuole Periphery 0.000138 0.000694 0.000045 0.001758 0.000098 0.000499 0.000201 0.001157 0.000105 0.000558 0.000112 0.001504

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.11 -3.40 -1.05 0.24 4.73 -0.07 6.84 3.14 3.13 -2.85 -0.28 1.16 2.14 2.50 1.29
Bud Neck 2.25 1.52 1.70 -1.45 0.36 -1.21 -2.43 -1.45 -0.03 1.09 0.22 -0.82 -0.07 -0.33 0.96
Bud Site 0.83 -2.00 -5.89 -8.45 -5.80 -0.47 -3.27 -6.82 -6.67 -4.54 0.21 -4.67 -10.33 -10.85 -7.37
Cell Periphery 2.50 -2.64 -6.17 -7.86 -4.98 -1.33 -4.04 -5.12 -2.87 0.72 -0.44 -5.74 -7.98 -7.41 -4.14
Cytoplasm 0.14 -2.12 -2.87 -2.88 -2.65 -2.24 -1.02 -3.69 -1.23 -2.58 -2.14 -0.91 -4.22 -1.87 -3.67
Cytoplasmic Foci -0.95 1.38 -2.24 -1.49 -2.78 -2.10 0.53 -4.05 -2.70 -4.29 -2.14 1.43 -4.20 -2.47 -5.03
Eisosomes 0.75 2.38 2.56 2.43 0.49 6.23 -4.94 8.02 2.18 12.75 4.90 -0.89 6.19 1.25 8.66
Endoplasmic Reticulum 1.64 2.53 1.69 0.06 -2.18 -3.95 -3.96 -5.63 -0.04 -2.49 -3.41 -0.67 -3.33 1.41 -3.18
Endosome 1.62 2.66 1.18 -1.05 -4.69 -6.69 -4.85 -7.76 -2.55 -5.80 -5.87 -0.87 -7.12 -0.60 -6.91
Golgi 0.12 3.54 2.35 2.63 -3.43 -5.23 -2.20 -6.42 -2.34 -5.35 -4.59 2.27 -3.78 1.10 -6.25
Lipid Particles -0.07 2.08 1.87 3.35 -1.82 -6.50 -5.56 -6.79 -1.17 -2.38 -5.76 -1.57 -3.61 2.80 -2.58
Mitochondria -0.13 0.34 -1.80 -2.08 -3.92 2.37 5.28 0.99 -1.08 -3.18 2.44 4.92 0.54 -2.11 -5.03
Mitotic Spindle 2.15 -1.33 -1.96 -2.42 -0.97 -2.27 0.30 -2.78 -1.73 -2.81 -2.17 -0.12 -2.92 -1.25 -2.92
None 0.03 4.85 1.16 0.97 -2.84 -0.92 -0.18 0.32 0.99 0.27 -0.90 1.54 1.33 1.28 -0.75
Nuclear Periphery -0.85 1.70 -0.64 0.69 -2.76 -3.36 -4.87 -1.74 -1.28 -1.24 -2.06 -2.60 -2.12 -0.69 -1.77
Nuclear Periphery Foci -0.55 1.14 -1.03 0.01 -2.64 -2.15 -1.91 -1.48 0.11 -1.36 -2.27 -0.40 -1.88 0.58 -1.84
Nucleolus 0.05 2.21 -1.82 -1.81 -2.18 -2.22 -1.26 -1.86 -1.73 -1.25 -2.18 -1.16 -2.03 -1.81 -1.44
Nucleus 2.58 3.25 -0.78 -3.37 -3.98 -1.40 -1.10 -2.39 -1.55 -2.22 -1.24 0.67 -2.59 -1.28 -2.68
Peroxisomes -1.35 1.59 -0.77 0.95 -1.54 -1.94 3.23 -1.14 0.84 -3.12 -2.10 3.68 -0.35 1.77 -3.32
Vacuole -0.94 -0.25 -3.20 -3.01 -3.18 -3.38 -3.27 -3.43 -2.35 -2.53 -3.51 -3.18 -4.71 -3.34 -4.03
Vacuole Periphery -1.69 2.24 -2.95 -1.68 -3.13 -3.07 -2.23 -3.81 -2.16 -3.41 -3.36 -0.30 -4.15 -2.61 -4.13