Standard name | |
---|---|
Description | Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family; human homolog DDX51 complements yeast dbp6 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 1 | 2 | 2 | 4 | 0 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 8 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 7 | 1 | 2 | 5 | 8 | 1 | – | 6 | 2 | 5 | 5 | 10 | 19 | 38 | 12 | 19 | 10 | 0 | 1 | 0 | 0 | 3 | 4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2 | 2 | 0 | 7 | 8 | 12 | 28 | – | 4 | 12 | 3 | 6 | 2 | 8 | 16 | 3 | 3 | 0 | 2 | 3 | 0 | 2 | 6 | 10 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 14 | 6 | 1 | 0 | 2 | 6 | 14 | – | 24 | 14 | 34 | 23 | 1 | 1 | 0 | 17 | 6 | 17 | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleus | 72 | 52 | 4 | 6 | 13 | 11 | 32 | – | 13 | 38 | 17 | 28 | 27 | 27 | 27 | 28 | 37 | 42 | 51 | 37 | 8 | 19 | 36 | 46 |
Nuclear Periphery | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 209 | 105 | 13 | 74 | 133 | 110 | 173 | – | 110 | 148 | 107 | 137 | 116 | 221 | 196 | 121 | 125 | 133 | 189 | 109 | 24 | 67 | 210 | 212 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 3 | 0 | – | 1 | 0 | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 6 | 0 | 0 | 22 | 47 | 42 | 65 | – | 34 | 61 | 30 | 47 | 19 | 40 | 46 | 5 | 3 | 6 | 5 | 1 | 0 | 3 | 20 | 26 |
Vac/Vac Membrane | 2 | 4 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 1 | 0 | 0 | 2 | 3 | 5 | 11 | 8 | 0 | 2 | 0 | 1 | 5 | 18 |
Unique Cell Count | 253 | 154 | 16 | 83 | 148 | 131 | 213 | 136 | 197 | 138 | 181 | 138 | 258 | 268 | 161 | 186 | 185 | 251 | 159 | 42 | 100 | 290 | 339 | |
Labelled Cell Count | 311 | 176 | 19 | 111 | 212 | 192 | 315 | 198 | 288 | 205 | 257 | 175 | 322 | 333 | 194 | 214 | 217 | 251 | 159 | 42 | 100 | 290 | 339 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 14.3 | 8.9 | 11.4 | 10.8 | 8.4 | 8.2 | – | 6.4 | 6.8 | 6.1 | 6.4 | 12.6 | 11.8 | 11.2 | 17.8 | 17.2 | 17.1 | 10.7 | 11.9 | 12.2 |
Std Deviation (1e-4) | 2.2 | 3.6 | 2.4 | 2.7 | 2.4 | 2.5 | 1.8 | – | 1.3 | 1.5 | 1.3 | 2.1 | 3.5 | 3.3 | 3.8 | 5.7 | 6.1 | 6.8 | 2.4 | 2.9 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.36 | 0.28 | -0.08 | -0.11 | – | -0.48 | -0.39 | -0.55 | -0.47 | 0.5 | 0.42 | 0.34 | 1.0 | 0.95 | 0.94 | 0.27 | 0.43 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.9 | 1.0 | 0.3 | 0 | – | 0 | -0.5 | -0.3 | -0.5 | 0.3 | 0.5 | -0.7 | -0.5 | -1.2 | -0.8 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |