Standard name
Human Ortholog
Description Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family; human homolog DDX51 complements yeast dbp6 mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0 0 0.06 0 0 0 0 0 0.07 0.07 0.14 0.07 0.1 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.08 0.05 0.09 0.13 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.06 0 0 0.05 0.07 0.18 0.07 0.25 0.13 0 0 0 0.11 0 0.09 0 0 0 0 0 0
Nucleus 0.28 0.34 0.25 0.07 0.09 0.08 0.15 0.1 0.19 0.12 0.15 0.2 0.1 0.1 0.17 0.2 0.23 0.21 0.23 0.21 0.2 0.13 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.83 0.68 0.81 0.89 0.9 0.84 0.81 0.81 0.75 0.78 0.76 0.84 0.86 0.73 0.75 0.67 0.72 0.76 0.69 0.58 0.67 0.72 0.63
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.27 0.32 0.32 0.31 0.25 0.31 0.22 0.26 0.14 0.16 0.17 0 0 0 0 0 0 0 0.07 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 2 1 5
Bud Neck 0 0 0 0 2 0 1 1 2 2 4 0 3 4 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 4 8 5 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 7 1 2 5 8 1 6 2 5 5 10 19 38 12 19 10 0 1 0 0 3 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 2 1 0 0 0 0 0 0
Endosome 2 2 0 7 8 12 28 4 12 3 6 2 8 16 3 3 0 2 3 0 2 6 10
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 3
Mitochondria 14 6 1 0 2 6 14 24 14 34 23 1 1 0 17 6 17 0 1 0 0 1 1
Nucleus 72 52 4 6 13 11 32 13 38 17 28 27 27 27 28 37 42 51 37 8 19 36 46
Nuclear Periphery 4 0 0 0 0 0 0 0 2 0 1 0 0 0 0 2 0 0 0 0 0 0 1
Nucleolus 209 105 13 74 133 110 173 110 148 107 137 116 221 196 121 125 133 189 109 24 67 210 212
Peroxisomes 1 0 0 0 0 3 0 1 0 1 1 0 1 3 0 1 0 0 0 1 0 0 0
SpindlePole 6 0 0 22 47 42 65 34 61 30 47 19 40 46 5 3 6 5 1 0 3 20 26
Vac/Vac Membrane 2 4 0 0 1 0 1 1 0 1 0 0 2 3 5 11 8 0 2 0 1 5 18
Unique Cell Count 253 154 16 83 148 131 213 136 197 138 181 138 258 268 161 186 185 251 159 42 100 290 339
Labelled Cell Count 311 176 19 111 212 192 315 198 288 205 257 175 322 333 194 214 217 251 159 42 100 290 339


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 14.3 8.9 11.4 10.8 8.4 8.2 6.4 6.8 6.1 6.4 12.6 11.8 11.2 17.8 17.2 17.1 10.7 11.9 12.2
Std Deviation (1e-4) 2.2 3.6 2.4 2.7 2.4 2.5 1.8 1.3 1.5 1.3 2.1 3.5 3.3 3.8 5.7 6.1 6.8 2.4 2.9 3.0
Intensity Change (Log2) 0.36 0.28 -0.08 -0.11 -0.48 -0.39 -0.55 -0.47 0.5 0.42 0.34 1.0 0.95 0.94 0.27 0.43 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.9 1.0 0.3 0 0 -0.5 -0.3 -0.5 0.3 0.5 -0.7 -0.5 -1.2 -0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6884 7.9268 6.8986 5.1195 5.7445 6.2216 7.3067 9.1371 7.8553 7.5848 6.2243 7.8942 7.1235 8.2019 6.6115 7.2643 6.0331 6.663
Actin 0.0258 0 0.012 0 0.0056 0.0057 0.0032 0 0.0008 0 0.0001 0.0002 0.0273 0 0.0221 0.0614 0.0068 0.0039
Bud 0.0003 0 0.0004 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0003 0 0.0007 0.0004 0.0003 0
Bud Neck 0.0005 0.0001 0.0003 0.0004 0.0003 0.002 0.0003 0.0002 0.0004 0.0007 0.0002 0.0005 0.0008 0.0002 0.0022 0.0012 0.0007 0.0005
Bud Periphery 0.0003 0 0.0008 0.0001 0.0011 0.0004 0.0002 0 0.0003 0 0.0001 0.0001 0.0005 0 0.0012 0.0006 0.0006 0.0001
Bud Site 0.0025 0.0002 0.001 0.0003 0.0005 0.0003 0.0009 0.0001 0.0004 0.0001 0.0002 0.0001 0.0059 0.0001 0.0051 0.0012 0.0009 0.0001
Cell Periphery 0.0005 0.0001 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0002 0.0001 0.0007 0.0001 0.0001 0
Cytoplasm 0.0019 0 0.0005 0.0001 0.0002 0.0001 0.001 0 0.0001 0 0.0001 0 0.0028 0 0.0006 0.0064 0.0003 0.0006
Cytoplasmic Foci 0.0142 0 0.0012 0.0054 0.0029 0.0048 0.0188 0.0006 0.0109 0.0002 0.0029 0.0006 0.0191 0.0009 0.0026 0.0065 0.0075 0.0013
Eisosomes 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0.0006 0 0.0005 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0003 0 0.001 0 0.0007 0.0005 0.0005 0 0.0003 0 0 0 0.0055 0 0.0005 0.0042 0.0004 0.0003
Endosome 0.008 0 0.0082 0.0002 0.0089 0.0048 0.0092 0.0004 0.0029 0 0.0002 0.0005 0.0086 0.0001 0.0049 0.0205 0.0067 0.0026
Golgi 0.0035 0 0.0036 0.0001 0.0051 0.006 0.0045 0.0004 0.0019 0 0.0003 0.0005 0.0037 0 0.0022 0.0099 0.005 0.0008
Lipid Particles 0.031 0.0001 0.004 0.0043 0.0139 0.0074 0.0318 0.0043 0.0082 0.0006 0.0125 0.0062 0.0089 0.0015 0.004 0.0042 0.0322 0.0019
Mitochondria 0.0266 0 0.013 0.001 0.0265 0.0143 0.0072 0.0112 0.007 0.0002 0.0072 0.0056 0.0041 0.0001 0.0044 0.0057 0.0056 0.0002
None 0.0013 0 0.0002 0.0003 0.0004 0.0002 0.0016 0 0.0001 0 0.0002 0 0.0017 0 0.0007 0.0026 0.0009 0.0069
Nuclear Periphery 0.0005 0 0.001 0.0004 0.0049 0.0029 0.0036 0.0007 0.0016 0.0007 0.0009 0.0002 0.0046 0 0.0014 0.0108 0.0033 0.002
Nucleolus 0.7677 0.8479 0.8523 0.9065 0.8102 0.8372 0.7631 0.8044 0.8121 0.8962 0.8198 0.8638 0.7502 0.8428 0.8352 0.6529 0.7639 0.843
Nucleus 0.0914 0.1511 0.0957 0.0725 0.0966 0.108 0.106 0.1728 0.1385 0.0975 0.1393 0.1122 0.1264 0.1529 0.1057 0.1971 0.104 0.1269
Peroxisomes 0.0067 0 0.0027 0.0003 0.001 0.0014 0.0163 0.0002 0.0026 0 0.0012 0.0004 0.0068 0.0001 0.0017 0.0063 0.0114 0.0006
Punctate Nuclear 0.0112 0.0003 0.0006 0.0056 0.0185 0.0019 0.0298 0.0037 0.0098 0.003 0.013 0.0088 0.0209 0.0005 0.0023 0.0067 0.0482 0.0078
Vacuole 0.0043 0.0001 0.0007 0.0013 0.0008 0.0008 0.0008 0.0003 0.0008 0.0002 0.0005 0.0001 0.0005 0.0005 0.001 0.0005 0.0005 0.0002
Vacuole Periphery 0.0013 0 0.0004 0.0008 0.0013 0.0009 0.0007 0.0004 0.001 0.0003 0.001 0.0001 0.0005 0.0001 0.0004 0.0005 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.4506 43.8389 30.9813 34.2763 30.2775 19.2551 31.3972 22.4375 21.5654 37.7116
Translational Efficiency 1.1599 1.0976 1.2686 0.9639 1.3439 1.707 0.9599 1.1688 1.1932 1.0826

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency