Standard name
Human Ortholog
Description Para hydroxybenzoate polyprenyl transferase; catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis; human COQ2, mutations in which are implicated in an increased risk of mutiple-system atrophy, can complement a yeast coq2 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0.05 0.05 0.06 0.05 0.08 0.06 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.08 0.29 0.21 0.22 0.17 0.17 0.16 0.15 0.16 0.14 0.13 0.1 0.59 0.62 0.74 – – – 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0.09 0.14 0.08 – – – 0.15 0.09 0.08 0.07 0.08 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.84 0.53 0.75 0.79 0.8 0.86 0.86 0.85 0.86 0.85 0.91 0.9 0.17 0.09 0 – – – 0.68 0.66 0.7 0.74 0.62 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.06 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0.08 0.06 0 0.05 0.06 0.08 0.06 0.11 0.08 0.09 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.15 0 0 0.08 0 0 0 0 0 0 0 0.13 0.15 0.1 – – – 0 0.07 0 0.06 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 0 0 1 1 – – – 2 7 7 2 10 12
Bud 1 4 13 11 20 36 35 41 32 44 47 44 1 4 1 – – – 0 2 0 1 2 5
Bud Neck 0 1 2 1 1 5 0 0 1 4 2 5 3 1 1 – – – 1 4 2 1 6 7
Bud Site 0 0 1 1 3 15 15 9 18 22 14 16 0 0 0 – – – – – – – – –
Cell Periphery 0 4 2 2 2 2 2 1 4 4 4 0 3 1 8 – – – 0 1 1 0 3 0
Cytoplasm 14 68 67 74 90 96 121 111 91 118 72 69 101 163 199 – – – 2 15 8 3 6 6
Endoplasmic Reticulum 4 10 1 1 0 1 0 1 1 1 1 1 6 3 3 – – – 0 3 5 2 4 7
Endosome 9 20 12 7 13 1 5 2 1 4 0 2 16 37 21 – – – 29 27 28 13 29 24
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – 5 5 2 2 10 8
Mitochondria 149 123 238 261 410 482 653 636 478 697 517 614 29 23 9 – – – 139 210 251 142 238 289
Nucleus 3 1 1 0 5 20 16 30 17 11 7 13 3 1 1 – – – 0 2 0 0 1 1
Nuclear Periphery 1 0 0 2 0 1 0 1 4 4 1 4 1 0 0 – – – 2 0 2 1 1 1
Nucleolus 0 1 1 0 0 2 2 7 4 5 4 2 0 0 1 – – – 0 0 1 0 2 2
Peroxisomes 3 1 17 18 14 2 1 4 2 10 3 7 5 16 11 – – – 7 6 14 6 13 8
SpindlePole 3 6 24 21 20 28 48 59 36 89 47 61 6 8 11 – – – 1 5 10 1 9 5
Vac/Vac Membrane 19 34 14 8 41 9 20 10 11 10 6 3 23 40 26 – – – 7 21 11 11 35 32
Unique Cell Count 177 233 319 330 515 558 756 750 556 817 568 682 171 264 269 205 317 360 193 385 423
Labelled Cell Count 206 275 393 407 619 700 918 912 700 1023 725 841 198 298 293 205 317 360 193 385 423


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.5 3.6 3.5 3.5 3.4 2.9 3.3 2.9 2.8 2.7 2.7 5.1 5.0 5.0 – – – 5.5 5.9 5.8
Std Deviation (1e-4) 0.6 0.7 1.0 1.3 1.3 1.4 1.3 1.6 1.3 0.9 1.0 1.0 1.3 1.1 1.0 – – – 1.1 1.6 1.3
Intensity Change (Log2) – – – -0.05 -0.02 -0.08 -0.29 -0.12 -0.29 -0.36 -0.41 -0.38 0.5 0.5 0.49 – – – 0.61 0.71 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5945 0.4357 0.3927 0.3125 0.4273 0.5192 2.0531 2.2537 1.9694 1.2927 2.3711 2.0464 -1.2476 -0.938 -0.9016 -0.8886 -1.1562 -0.8998
Actin 0.0109 0.0067 0.0118 0.0047 0.0158 0.011 0.0354 0.0146 0.0142 0.0054 0.0323 0.0125 0.0138 0.0127 0.0088 0.0459 0.0023 0.0045
Bud 0.0014 0.0118 0.0023 0.0113 0.0052 0.0035 0.0017 0.0023 0.0024 0.0037 0.0017 0.001 0.0008 0.0015 0.0009 0.0025 0.0006 0.0007
Bud Neck 0.0027 0.0013 0.0022 0.0029 0.0041 0.0073 0.0015 0.0023 0.0081 0.0423 0.0024 0.0052 0.0024 0.0036 0.0082 0.0026 0.0005 0.0036
Bud Periphery 0.0036 0.0079 0.0055 0.008 0.0083 0.0028 0.0031 0.004 0.0094 0.0152 0.0051 0.0043 0.0025 0.0045 0.003 0.0078 0.0011 0.0031
Bud Site 0.0133 0.0269 0.0121 0.0148 0.0015 0.0021 0.0056 0.041 0.0114 0.0038 0.0019 0.0027 0.0084 0.0287 0.0053 0.0043 0.0009 0.0009
Cell Periphery 0.0017 0.0014 0.0015 0.0028 0.0008 0.0006 0.0013 0.0014 0.0055 0.0017 0.0008 0.0045 0.0022 0.0021 0.0013 0.0018 0.0003 0.0011
Cytoplasm 0.0212 0.0315 0.0166 0.0218 0.0251 0.0275 0.0313 0.0272 0.0335 0.0292 0.0209 0.0251 0.0197 0.0186 0.021 0.0398 0.0073 0.0178
Cytoplasmic Foci 0.1101 0.0882 0.0745 0.0582 0.0726 0.0796 0.0995 0.093 0.0782 0.0528 0.0842 0.0927 0.085 0.1342 0.0776 0.0654 0.0356 0.084
Eisosomes 0.001 0.0007 0.0008 0.0006 0.0014 0.0005 0.0012 0.0013 0.0029 0.0005 0.0008 0.0009 0.002 0.0025 0.0025 0.0017 0.0002 0.0009
Endoplasmic Reticulum 0.0097 0.0054 0.0011 0.0016 0.0035 0.0021 0.0065 0.0014 0.0023 0.0014 0.0021 0.0015 0.0034 0.0009 0.0031 0.0026 0.001 0.0015
Endosome 0.1236 0.1296 0.0831 0.1297 0.0879 0.0901 0.1167 0.1055 0.064 0.045 0.1123 0.0697 0.1122 0.0912 0.079 0.0608 0.1597 0.078
Golgi 0.0523 0.0695 0.0351 0.052 0.0407 0.041 0.0654 0.0903 0.0408 0.0226 0.0621 0.0502 0.0535 0.0681 0.0307 0.042 0.0661 0.053
Lipid Particles 0.0684 0.0193 0.0271 0.0251 0.0204 0.0292 0.0536 0.0373 0.0131 0.0099 0.0173 0.0249 0.1025 0.0277 0.0449 0.0168 0.0086 0.0493
Mitochondria 0.3844 0.4141 0.5516 0.5891 0.4706 0.5122 0.3629 0.4428 0.5164 0.5837 0.4604 0.5203 0.4081 0.4582 0.5521 0.461 0.5512 0.5121
None 0.0803 0.079 0.0956 0.0423 0.1163 0.0805 0.0962 0.0515 0.0844 0.0836 0.0951 0.0665 0.0756 0.0403 0.0627 0.1267 0.0668 0.069
Nuclear Periphery 0.0077 0.0035 0.0011 0.0012 0.0034 0.0029 0.0061 0.0005 0.0008 0.0007 0.007 0.0007 0.0051 0.0002 0.001 0.0062 0.0004 0.0004
Nucleolus 0.004 0.0038 0.0021 0.001 0.0013 0.0016 0.002 0.0008 0.0027 0.0017 0.001 0.0008 0.0014 0.0029 0.007 0.0038 0.0003 0.0009
Nucleus 0.0031 0.0035 0.0017 0.0015 0.0067 0.0035 0.0047 0.0008 0.0021 0.0013 0.0035 0.0008 0.0019 0.0014 0.0016 0.0033 0.0004 0.0008
Peroxisomes 0.0788 0.0748 0.0583 0.0191 0.0978 0.0858 0.0872 0.0723 0.0978 0.0829 0.0737 0.1078 0.0868 0.0933 0.0749 0.0877 0.0829 0.1072
Punctate Nuclear 0.0073 0.0048 0.0064 0.002 0.0054 0.0047 0.0039 0.0018 0.0011 0.0023 0.0074 0.0014 0.0025 0.0013 0.001 0.0074 0.0003 0.0014
Vacuole 0.0102 0.0107 0.0066 0.0062 0.0057 0.0075 0.0097 0.0053 0.0063 0.0075 0.0043 0.0041 0.007 0.0043 0.0091 0.0059 0.0076 0.0064
Vacuole Periphery 0.0042 0.0055 0.003 0.0042 0.0054 0.0039 0.0046 0.0026 0.0027 0.0028 0.0035 0.0024 0.0035 0.0018 0.0044 0.0041 0.0058 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.2561 22.9185 24.8557 23.5647 24.291 27.5945 18.7165 21.054 30.929 23.2238
Translational Efficiency 1.1598 0.7593 0.8593 1.2205 0.787 0.7011 0.9954 1.0261 0.8213 0.7779

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
517 1677 1236 1385 1936 1491 1638 2512 2453 3168 2874 3897

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 682.11 726.40 868.72 814.70 662.53 732.57 789.27 852.01 666.66 729.30 823.44 838.75
Standard Deviation 114.15 100.07 107.37 112.05 84.72 91.36 89.23 119.76 92.06 96.12 105.09 118.43
Intensity Change Log 2 — 0.090760 0.348887 0.256264 — 0.144981 0.252533 0.362885 — 0.117730 0.302215 0.309783

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000556 0.001850 0.001562 0.008024 0.000487 0.001432 0.004045 0.001362 0.000501 0.001653 0.002977 0.003730
Bud Neck 0.054751 0.028864 0.019765 0.028868 0.028971 0.025607 0.042842 0.056136 0.034405 0.027331 0.032918 0.046445
Bud Site 0.041500 0.028101 0.025903 0.059814 0.017372 0.013850 0.057694 0.058989 0.022457 0.021394 0.044022 0.059283
Cell Periphery 0.000744 0.000355 0.000147 0.000358 0.000236 0.000291 0.000195 0.000294 0.000343 0.000325 0.000175 0.000317
Cytoplasm 0.084918 0.042463 0.258710* 0.276090* 0.030451 0.031124 0.227172* 0.277649* 0.041930 0.037126 0.240735* 0.277095*
Cytoplasmic Foci 0.231869* 0.181108* 0.188968* 0.119995 0.140485 0.145339 0.190853* 0.113844 0.159745 0.164273 0.190042* 0.116030
Eisosomes 0.000301 0.000222 0.000087 0.000185 0.000175 0.000170 0.000156 0.000135 0.000202 0.000198 0.000127 0.000153
Endoplasmic Reticulum 0.001211 0.000925 0.008171 0.004699 0.001057 0.000638 0.003874 0.002301 0.001090 0.000790 0.005722 0.003153
Endosome 0.087404 0.080598 0.046864 0.026558 0.093318 0.108436 0.026133 0.036892 0.092072 0.093700 0.035049 0.033219
Golgi 0.063970 0.109068 0.016656 0.009347 0.095198 0.110061* 0.015525 0.012386 0.088617 0.109535 0.016011 0.011306
Lipid Particles 0.025402 0.011089 0.011567 0.011683 0.013780 0.020393 0.007707 0.009553 0.016229 0.015468 0.009367 0.010310
Mitochondria 0.258495* 0.367472* 0.019592 0.027269 0.473892* 0.405501* 0.049061 0.060978 0.428494* 0.385370* 0.036388 0.048998
Mitotic Spindle 0.005561 0.013695 0.074288 0.042966 0.008189 0.009829 0.053355 0.050283 0.007635 0.011876 0.062358 0.047682
None 0.005014 0.002406 0.002303 0.003304 0.001550 0.002182 0.002199 0.002435 0.002280 0.002300 0.002244 0.002744
Nuclear Periphery 0.000928 0.000187 0.005153 0.002984 0.000333 0.000334 0.001407 0.001515 0.000459 0.000256 0.003018 0.002037
Nuclear Periphery Foci 0.000326 0.000234 0.004609 0.001540 0.000236 0.000409 0.001126 0.000588 0.000255 0.000316 0.002624 0.000927
Nucleolus 0.001851 0.002025 0.005690 0.004354 0.002119 0.003893 0.003387 0.002917 0.002063 0.002904 0.004377 0.003427
Nucleus 0.023208 0.007236 0.150961* 0.169415* 0.007097 0.008540 0.132061* 0.114550 0.010492 0.007850 0.140189* 0.134049*
Peroxisomes 0.070657 0.083792 0.021699 0.046235 0.036841 0.055067 0.091880 0.036610 0.043968 0.070273 0.061698 0.040031
Vacuole 0.027932 0.026701 0.127983 0.151910 0.031359 0.038142 0.085426 0.153555 0.030637 0.032086 0.103728 0.152970
Vacuole Periphery 0.013401 0.011607 0.009323 0.004403 0.016854 0.018763 0.003900 0.007027 0.016126 0.014975 0.006232 0.006094

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.06 -5.29 -6.57 -5.10 -5.27 -2.17 -9.06 -6.14 0.30 6.66 -4.71 -10.45 -7.81 -3.85 -0.79
Bud Neck 4.34 6.03 4.70 0.68 -3.15 1.18 -4.89 -8.93 -9.84 -4.10 2.90 0.57 -4.22 -8.38 -5.97
Bud Site 2.28 2.66 -1.69 -6.30 -6.67 2.01 -11.61 -13.64 -15.00 0.02 0.56 -7.91 -12.48 -14.22 -4.25
Cell Periphery 2.70 4.20 3.03 1.09 -5.04 -1.87 2.08 -1.18 0.86 -3.76 0.48 4.96 1.68 1.75 -6.36
Cytoplasm 5.68 -16.69 -18.69 -30.52 -1.79 -0.29 -31.36 -42.86 -41.50 -6.45 1.69 -38.60 -48.46 -51.14 -6.05
Cytoplasmic Foci 5.00 4.77 12.33 12.05 10.77 -1.01 -7.99 6.33 6.77 13.99 -1.07 -5.02 11.61 13.87 17.61
Eisosomes 2.96 8.64 4.89 3.01 -7.60 0.28 1.33 3.32 4.67 3.90 0.28 6.14 4.56 6.00 -2.84
Endoplasmic Reticulum 0.78 -12.91 -8.47 -11.55 6.94 2.38 -8.13 -3.55 -5.45 4.19 1.69 -15.48 -7.95 -10.04 8.19
Endosome 1.02 6.92 10.97 17.63 9.06 -2.97 20.12 17.14 17.94 -4.59 -0.43 19.48 20.99 24.08 2.43
Golgi -6.98 8.94 10.60 26.55 4.93 -2.67 23.48 24.97 22.57 2.33 -5.16 25.07 27.32 34.50 4.76
Lipid Particles 5.08 4.96 5.10 0.46 0.81 -3.16 4.59 3.29 5.96 -1.29 0.49 5.69 5.17 5.09 -0.10
Mitochondria -6.98 17.96 17.64 40.71* -1.07 6.04 51.94* 51.21* 39.10* -2.37 4.82 54.99* 53.52* 54.95* -3.46
Mitotic Spindle -4.88 -13.51 -9.42 -6.93 5.55 -1.28 -12.33 -13.91 -13.05 1.02 -3.78 -17.95 -16.48 -14.28 4.50
None 1.50 1.74 1.30 -0.47 -1.16 -1.21 -1.56 -1.70 0.33 -0.09 -0.32 0.14 -0.37 0.04 -0.83
Nuclear Periphery 1.52 -6.21 -3.39 -7.42 3.60 -0.02 -8.55 -5.65 -5.83 -0.50 1.68 -10.36 -7.33 -9.45 3.31
Nuclear Periphery Foci 0.90 -10.95 -7.29 -8.97 7.38 -3.06 -9.25 -4.12 -1.97 4.68 -1.54 -13.41 -8.70 -8.04 8.95
Nucleolus -0.42 -5.57 -3.40 -2.86 2.33 -2.26 -2.72 -1.82 1.19 1.15 -1.72 -5.43 -3.17 -0.90 2.77
Nucleus 4.01 -18.68 -21.30 -28.34 -1.96 -1.18 -25.95 -29.25 -28.56 3.10 2.00 -34.19 -37.90 -39.99 1.62
Peroxisomes -2.02 8.55 5.07 9.47 -5.15 -5.12 -11.23 0.84 5.88 11.89 -8.42 -4.76 3.12 11.78 7.84
Vacuole 0.34 -19.80 -24.66 -26.43 -7.57 -2.42 -18.08 -31.44 -28.18 -14.62 -0.71 -26.90 -39.95 -39.62 -15.07
Vacuole Periphery 0.95 2.50 5.62 9.56 5.34 -1.28 14.10 10.34 10.17 -4.59 1.14 11.62 12.48 12.92 0.92
Standard name
Human Ortholog
Description Para hydroxybenzoate polyprenyl transferase; catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis; human COQ2, mutations in which are implicated in an increased risk of mutiple-system atrophy, can complement a yeast coq2 null mutant
Localization
Cell Percentages mitochondrion (76%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Coq2

Coq2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coq2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available