Standard name
Description Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.98 0.99 0.96 0.91 0.85 0.64 0.63 0.51 0.58 0.97 0.89 0.86 0.97 0.94 0.95 0.85 0.89 0.83 0.78 0.75 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.12 0.35 0.39 0.48 0.35 0 0 0 0 0 0 0.05 0 0.07 0.09 0.09 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 1 5 4 28 25 14 10 13 4 10 10 1 0 0 1 1 4 2 7 3
Bud 4 3 2 1 2 6 18 24 24 18 26 2 13 12 0 0 0 3 4 3 2 7 12
Bud Neck 3 0 13 10 18 6 21 6 1 2 3 0 0 0 0 1 1 1 1 2 1 3 4
Bud Site 0 0 0 0 0 1 4 2 2 2 1 1 0 1 0 0 0
Cell Periphery 2 3 9 5 7 5 13 20 28 21 43 2 8 18 10 5 6 1 0 7 2 3 2
Cytoplasm 349 329 479 430 480 576 680 343 337 238 311 368 315 353 338 247 225 315 307 405 217 259 298
Endoplasmic Reticulum 2 3 6 2 2 1 9 7 11 7 8 2 3 8 24 14 16 2 1 2 3 7 3
Endosome 2 0 0 0 0 4 5 2 2 1 5 1 7 14 3 4 3 5 3 6 2 1 1
Golgi 1 0 0 0 0 0 0 1 0 1 0 0 0 0 2 1 1 1 1 1 1 0 2
Mitochondria 6 4 0 3 2 48 94 188 211 224 185 4 6 10 8 4 4 20 11 35 25 30 22
Nucleus 2 3 1 0 2 3 1 3 3 4 2 1 0 3 1 0 0 0 1 1 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 4 1 2 0 0 0 1 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 9 7 4 2 1 3 0 7 3 0 0 0 0 0 0 0 0 1
SpindlePole 2 0 3 2 10 11 35 16 7 3 15 1 4 9 1 2 1 9 1 7 1 2 3
Vac/Vac Membrane 1 0 1 0 0 8 7 4 16 28 28 1 2 4 2 6 6 4 4 2 13 16 14
Unique Cell Count 363 334 489 436 501 632 796 533 539 464 534 378 353 412 350 262 237 373 344 489 278 346 378
Labelled Cell Count 374 345 517 455 528 682 922 645 662 562 646 387 375 445 391 285 264 373 344 489 278 346 378


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.9 5.0 4.3 4.9 4.2 4.3 4.1 4.3 4.1 4.8 4.3 4.9 5.0 6.0 6.2 6.4 3.9 4.1 4.5
Std Deviation (1e-4) 1.7 1.0 0.9 1.0 1.1 1.2 1.1 1.2 1.2 1.0 1.1 1.0 1.7 1.4 1.4 1.6 1.9 1.1 1.3 1.8
Intensity Change (Log2) -0.23 -0.03 -0.26 -0.21 -0.27 -0.21 -0.28 -0.04 -0.21 -0.03 0.0 0.28 0.31 0.37 -0.36 -0.29 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available