Standard name | |
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Description | Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 8 | 5 | 3 | 1 | – | 1 | 4 | 9 | 11 | 10 | 18 | 20 |
Bud | 2 | 10 | 8 | 11 | 17 | 10 | 28 | 32 | – | 26 | 21 | 16 | 1 | 0 | 0 | 4 | – | 5 | 0 | 2 | 3 | 4 | 8 | 14 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 5 | 1 | 3 | 2 | – | 1 | 1 | 3 | 3 | 5 | 13 | 10 |
Bud Site | 0 | 0 | 3 | 0 | 1 | 1 | 6 | 8 | – | 14 | 10 | 9 | 0 | 0 | 0 | 0 | – | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 12 | 3 | 3 | 2 | 2 | 0 | – | 2 | 3 | 1 | 5 | 6 | 0 | 1 | – | 2 | 0 | 0 | 1 | 1 | 2 | 6 |
Cytoplasm | 22 | 60 | 40 | 32 | 32 | 29 | 31 | 30 | – | 17 | 26 | 16 | 131 | 203 | 254 | 31 | – | 32 | 3 | 8 | 1 | 14 | 22 | 29 |
Endoplasmic Reticulum | 7 | 7 | 2 | 2 | 1 | 3 | 0 | 2 | – | 1 | 0 | 1 | 9 | 21 | 1 | 10 | – | 8 | 0 | 2 | 4 | 10 | 6 | 7 |
Endosome | 16 | 8 | 37 | 29 | 77 | 11 | 5 | 7 | – | 1 | 0 | 1 | 10 | 4 | 10 | 5 | – | 15 | 28 | 28 | 48 | 31 | 39 | 31 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 4 | 5 | 7 | 8 | 1 | 4 | 3 |
Mitochondria | 76 | 184 | 226 | 206 | 209 | 290 | 449 | 427 | – | 378 | 347 | 372 | 3 | 6 | 2 | 12 | – | 14 | 130 | 187 | 270 | 239 | 327 | 285 |
Nucleus | 3 | 3 | 0 | 3 | 0 | 7 | 11 | 12 | – | 4 | 3 | 2 | 3 | 0 | 1 | 2 | – | 2 | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 0 | 4 | 5 | – | 4 | 3 | 4 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 4 | 2 | 3 | 3 |
Nucleolus | 1 | 0 | 1 | 1 | 1 | 0 | 12 | 10 | – | 4 | 2 | 3 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 1 | 0 | 3 | 2 |
Peroxisomes | 1 | 0 | 38 | 25 | 39 | 10 | 16 | 10 | – | 2 | 0 | 9 | 1 | 4 | 2 | 1 | – | 7 | 6 | 6 | 22 | 11 | 11 | 5 |
SpindlePole | 9 | 4 | 26 | 19 | 18 | 11 | 38 | 40 | – | 21 | 8 | 19 | 4 | 2 | 4 | 14 | – | 16 | 3 | 7 | 11 | 16 | 17 | 12 |
Vac/Vac Membrane | 22 | 16 | 25 | 9 | 29 | 13 | 16 | 17 | – | 8 | 9 | 9 | 11 | 6 | 12 | 11 | – | 11 | 8 | 22 | 3 | 17 | 25 | 20 |
Unique Cell Count | 137 | 247 | 334 | 267 | 324 | 319 | 500 | 484 | 398 | 365 | 391 | 176 | 244 | 284 | 79 | 97 | 203 | 295 | 407 | 382 | 516 | 464 | ||
Labelled Cell Count | 163 | 297 | 420 | 342 | 427 | 387 | 618 | 601 | 482 | 432 | 463 | 191 | 258 | 292 | 96 | 119 | 203 | 295 | 407 | 382 | 516 | 464 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 4.2 | 4.1 | 4.1 | 3.9 | 3.3 | 3.2 | 3.5 | – | 2.9 | 3.2 | 3.2 | 5.3 | 5.2 | 5.6 | 5.2 | 4.6 | 5.7 | 5.1 | 5.1 | 5.6 |
Std Deviation (1e-4) | 0.9 | 0.8 | 1.2 | 1.5 | 1.2 | 1.2 | 1.3 | 1.3 | – | 0.8 | 1.2 | 1.1 | 1.1 | 0.8 | 1.3 | 2.2 | 1.3 | 2.1 | 1.7 | 0.9 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.07 | -0.33 | -0.39 | -0.25 | – | -0.51 | -0.38 | -0.36 | 0.36 | 0.33 | 0.44 | 0.32 | 0.16 | 0.47 | 0.31 | 0.3 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.8 | – | 1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9517 | 0.8204 | 1.0375 | 1.2469 | 0.3491 | 1.2231 | 1.4279 | 1.9096 | 1.8521 | 2.512 | 1.9057 | 1.7318 | 2.1138 | 2.2054 | 2.2013 | 1.9475 | 2.3188 | 2.3067 |
Actin | 0.0236 | 0.0042 | 0.0145 | 0.0071 | 0.0058 | 0.0049 | 0.0262 | 0.0039 | 0.0434 | 0.0574 | 0.03 | 0.0175 | 0.022 | 0.0151 | 0.0183 | 0.0073 | 0.0013 | 0.0124 |
Bud | 0.0016 | 0.003 | 0.0044 | 0.0026 | 0.0032 | 0.001 | 0.0022 | 0.0041 | 0.0026 | 0.0038 | 0.0064 | 0.0015 | 0.0018 | 0.0025 | 0.0026 | 0.0028 | 0.0007 | 0.0012 |
Bud Neck | 0.0019 | 0.0018 | 0.0097 | 0.0007 | 0.0022 | 0.01 | 0.0072 | 0.0021 | 0.0057 | 0.0037 | 0.002 | 0.0119 | 0.0056 | 0.0063 | 0.0055 | 0.017 | 0.0003 | 0.0046 |
Bud Periphery | 0.0089 | 0.0091 | 0.0082 | 0.0098 | 0.0086 | 0.0028 | 0.0068 | 0.01 | 0.0079 | 0.0108 | 0.0119 | 0.0069 | 0.0031 | 0.0048 | 0.0074 | 0.0143 | 0.0046 | 0.0036 |
Bud Site | 0.0043 | 0.0167 | 0.0092 | 0.0031 | 0.0056 | 0.0017 | 0.0141 | 0.0418 | 0.0162 | 0.0072 | 0.012 | 0.0024 | 0.0124 | 0.0548 | 0.0155 | 0.0039 | 0.0004 | 0.0021 |
Cell Periphery | 0.0015 | 0.0021 | 0.002 | 0.0011 | 0.0011 | 0.0008 | 0.0072 | 0.006 | 0.0058 | 0.0027 | 0.0023 | 0.0017 | 0.0022 | 0.0034 | 0.0036 | 0.0061 | 0.0007 | 0.0015 |
Cytoplasm | 0.0614 | 0.1348 | 0.0668 | 0.0999 | 0.0659 | 0.0689 | 0.0565 | 0.0985 | 0.0677 | 0.0866 | 0.1094 | 0.1041 | 0.0724 | 0.1273 | 0.0686 | 0.1244 | 0.033 | 0.0973 |
Cytoplasmic Foci | 0.1288 | 0.1204 | 0.1102 | 0.0629 | 0.0956 | 0.1176 | 0.151 | 0.1713 | 0.1362 | 0.2105 | 0.1317 | 0.1898 | 0.1496 | 0.1479 | 0.1359 | 0.0802 | 0.1366 | 0.1642 |
Eisosomes | 0.001 | 0.0006 | 0.0017 | 0.0006 | 0.0003 | 0.0005 | 0.0018 | 0.0014 | 0.0029 | 0.0011 | 0.0008 | 0.0008 | 0.0012 | 0.0008 | 0.0022 | 0.0009 | 0.0003 | 0.0008 |
Endoplasmic Reticulum | 0.0083 | 0.0085 | 0.0057 | 0.0036 | 0.0014 | 0.0048 | 0.0047 | 0.0023 | 0.0045 | 0.0035 | 0.0077 | 0.0026 | 0.0098 | 0.0033 | 0.0038 | 0.0023 | 0.0062 | 0.0079 |
Endosome | 0.1552 | 0.1583 | 0.1154 | 0.1245 | 0.177 | 0.1274 | 0.1515 | 0.1308 | 0.0731 | 0.0636 | 0.1342 | 0.1224 | 0.183 | 0.1308 | 0.1247 | 0.0619 | 0.2094 | 0.1294 |
Golgi | 0.0309 | 0.0284 | 0.0338 | 0.0407 | 0.0513 | 0.0387 | 0.059 | 0.0449 | 0.0391 | 0.0333 | 0.0591 | 0.0431 | 0.0752 | 0.071 | 0.0523 | 0.0517 | 0.0618 | 0.0583 |
Lipid Particles | 0.0724 | 0.0294 | 0.0365 | 0.0526 | 0.0097 | 0.0384 | 0.0541 | 0.0176 | 0.0223 | 0.0106 | 0.0275 | 0.0301 | 0.0483 | 0.0185 | 0.0188 | 0.0033 | 0.038 | 0.0239 |
Mitochondria | 0.2107 | 0.1846 | 0.2603 | 0.3019 | 0.3944 | 0.2401 | 0.183 | 0.1872 | 0.3099 | 0.1698 | 0.1228 | 0.1855 | 0.2353 | 0.2024 | 0.3269 | 0.4825 | 0.3665 | 0.2831 |
None | 0.1701 | 0.2266 | 0.2438 | 0.2274 | 0.1281 | 0.2618 | 0.2122 | 0.2297 | 0.1703 | 0.2516 | 0.2897 | 0.1829 | 0.0949 | 0.1575 | 0.1428 | 0.1132 | 0.0689 | 0.1312 |
Nuclear Periphery | 0.0194 | 0.0022 | 0.0057 | 0.0028 | 0.0006 | 0.003 | 0.0021 | 0.0005 | 0.0012 | 0.0005 | 0.0013 | 0.0008 | 0.0043 | 0.002 | 0.0008 | 0.0002 | 0.0017 | 0.002 |
Nucleolus | 0.003 | 0.0035 | 0.0019 | 0.0011 | 0.0009 | 0.0016 | 0.0019 | 0.0027 | 0.0015 | 0.001 | 0.0007 | 0.0006 | 0.0014 | 0.0014 | 0.001 | 0.0006 | 0.0003 | 0.0005 |
Nucleus | 0.0127 | 0.0063 | 0.0037 | 0.0023 | 0.0012 | 0.0019 | 0.0017 | 0.0022 | 0.0026 | 0.001 | 0.0011 | 0.0007 | 0.0021 | 0.0019 | 0.0012 | 0.0009 | 0.0006 | 0.0011 |
Peroxisomes | 0.0462 | 0.0241 | 0.0391 | 0.0365 | 0.032 | 0.0563 | 0.0381 | 0.028 | 0.0728 | 0.07 | 0.0365 | 0.0817 | 0.0513 | 0.0259 | 0.055 | 0.0188 | 0.0518 | 0.0645 |
Punctate Nuclear | 0.016 | 0.0047 | 0.0034 | 0.0018 | 0.0016 | 0.0028 | 0.0031 | 0.0018 | 0.0049 | 0.0025 | 0.0029 | 0.003 | 0.0041 | 0.0044 | 0.001 | 0.0002 | 0.0016 | 0.0017 |
Vacuole | 0.0162 | 0.0252 | 0.0181 | 0.0105 | 0.0085 | 0.0105 | 0.0124 | 0.0108 | 0.0069 | 0.0065 | 0.007 | 0.0069 | 0.0149 | 0.0145 | 0.0085 | 0.0057 | 0.0102 | 0.0055 |
Vacuole Periphery | 0.0058 | 0.0056 | 0.006 | 0.0065 | 0.005 | 0.0043 | 0.0031 | 0.0023 | 0.0024 | 0.0021 | 0.0029 | 0.003 | 0.0051 | 0.0033 | 0.0034 | 0.0019 | 0.0052 | 0.0033 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.3778 | 3.355 | 6.4729 | 8.1598 | 7.7856 | 6.7518 | 9.3287 | 11.9056 | 10.1586 | 10.2161 |
Translational Efficiency | 0.947 | 0.7488 | 0.5037 | 0.5244 | 0.3878 | 0.5708 | 0.4475 | 0.4342 | 0.4686 | 0.4693 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |