Standard name
Description Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0.06 0.07 – 0.07 0.06 0 0 0 0 0.05 – 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.16 0.24 0.12 0.12 0.1 0.09 0.06 0.06 – 0 0.07 0 0.74 0.83 0.89 0.39 – 0.33 0 0 0 0 0 0.06
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 – 0 0 0 0.05 0.09 0 0.13 – 0.08 0 0 0 0 0 0
Endosome 0.12 0 0.11 0.11 0.24 0 0 0 – 0 0 0 0.06 0 0 0.06 – 0.15 0.14 0.1 0.12 0.08 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.55 0.74 0.68 0.77 0.65 0.91 0.9 0.88 – 0.95 0.95 0.95 0 0 0 0.15 – 0.14 0.64 0.64 0.66 0.63 0.64 0.62
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.09 0.12 0 0 0 – 0 0 0 0 0 0 0 – 0.07 0 0 0.06 0 0 0
SpindlePole 0.07 0 0.08 0.07 0.06 0 0.08 0.08 – 0.05 0 0.05 0 0 0 0.18 – 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.06 0.07 0 0.09 0 0 0 – 0 0 0 0.06 0 0 0.14 – 0.11 0 0.08 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 0 0 0 0 0 – 0 0 0 8 5 3 1 – 1 4 9 11 10 18 20
Bud 2 10 8 11 17 10 28 32 – 26 21 16 1 0 0 4 – 5 0 2 3 4 8 14
Bud Neck 0 0 2 0 0 0 0 1 – 0 0 1 5 1 3 2 – 1 1 3 3 5 13 10
Bud Site 0 0 3 0 1 1 6 8 – 14 10 9 0 0 0 0 – 1 – – – – – –
Cell Periphery 0 2 12 3 3 2 2 0 – 2 3 1 5 6 0 1 – 2 0 0 1 1 2 6
Cytoplasm 22 60 40 32 32 29 31 30 – 17 26 16 131 203 254 31 – 32 3 8 1 14 22 29
Endoplasmic Reticulum 7 7 2 2 1 3 0 2 – 1 0 1 9 21 1 10 – 8 0 2 4 10 6 7
Endosome 16 8 37 29 77 11 5 7 – 1 0 1 10 4 10 5 – 15 28 28 48 31 39 31
Golgi 1 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 – 4 5 7 8 1 4 3
Mitochondria 76 184 226 206 209 290 449 427 – 378 347 372 3 6 2 12 – 14 130 187 270 239 327 285
Nucleus 3 3 0 3 0 7 11 12 – 4 3 2 3 0 1 2 – 2 1 0 0 0 0 1
Nuclear Periphery 1 1 0 1 0 0 4 5 – 4 3 4 0 0 0 0 – 0 1 1 4 2 3 3
Nucleolus 1 0 1 1 1 0 12 10 – 4 2 3 0 0 0 1 – 0 0 2 1 0 3 2
Peroxisomes 1 0 38 25 39 10 16 10 – 2 0 9 1 4 2 1 – 7 6 6 22 11 11 5
SpindlePole 9 4 26 19 18 11 38 40 – 21 8 19 4 2 4 14 – 16 3 7 11 16 17 12
Vac/Vac Membrane 22 16 25 9 29 13 16 17 – 8 9 9 11 6 12 11 – 11 8 22 3 17 25 20
Unique Cell Count 137 247 334 267 324 319 500 484 398 365 391 176 244 284 79 97 203 295 407 382 516 464
Labelled Cell Count 163 297 420 342 427 387 618 601 482 432 463 191 258 292 96 119 203 295 407 382 516 464


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 4.1 4.1 3.9 3.3 3.2 3.5 – 2.9 3.2 3.2 5.3 5.2 5.6 5.2 4.6 5.7 5.1 5.1 5.6
Std Deviation (1e-4) 0.9 0.8 1.2 1.5 1.2 1.2 1.3 1.3 – 0.8 1.2 1.1 1.1 0.8 1.3 2.2 1.3 2.1 1.7 0.9 1.6
Intensity Change (Log2) – – – -0.01 -0.07 -0.33 -0.39 -0.25 – -0.51 -0.38 -0.36 0.36 0.33 0.44 0.32 0.16 0.47 0.31 0.3 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 1.8 – 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9517 0.8204 1.0375 1.2469 0.3491 1.2231 1.4279 1.9096 1.8521 2.512 1.9057 1.7318 2.1138 2.2054 2.2013 1.9475 2.3188 2.3067
Actin 0.0236 0.0042 0.0145 0.0071 0.0058 0.0049 0.0262 0.0039 0.0434 0.0574 0.03 0.0175 0.022 0.0151 0.0183 0.0073 0.0013 0.0124
Bud 0.0016 0.003 0.0044 0.0026 0.0032 0.001 0.0022 0.0041 0.0026 0.0038 0.0064 0.0015 0.0018 0.0025 0.0026 0.0028 0.0007 0.0012
Bud Neck 0.0019 0.0018 0.0097 0.0007 0.0022 0.01 0.0072 0.0021 0.0057 0.0037 0.002 0.0119 0.0056 0.0063 0.0055 0.017 0.0003 0.0046
Bud Periphery 0.0089 0.0091 0.0082 0.0098 0.0086 0.0028 0.0068 0.01 0.0079 0.0108 0.0119 0.0069 0.0031 0.0048 0.0074 0.0143 0.0046 0.0036
Bud Site 0.0043 0.0167 0.0092 0.0031 0.0056 0.0017 0.0141 0.0418 0.0162 0.0072 0.012 0.0024 0.0124 0.0548 0.0155 0.0039 0.0004 0.0021
Cell Periphery 0.0015 0.0021 0.002 0.0011 0.0011 0.0008 0.0072 0.006 0.0058 0.0027 0.0023 0.0017 0.0022 0.0034 0.0036 0.0061 0.0007 0.0015
Cytoplasm 0.0614 0.1348 0.0668 0.0999 0.0659 0.0689 0.0565 0.0985 0.0677 0.0866 0.1094 0.1041 0.0724 0.1273 0.0686 0.1244 0.033 0.0973
Cytoplasmic Foci 0.1288 0.1204 0.1102 0.0629 0.0956 0.1176 0.151 0.1713 0.1362 0.2105 0.1317 0.1898 0.1496 0.1479 0.1359 0.0802 0.1366 0.1642
Eisosomes 0.001 0.0006 0.0017 0.0006 0.0003 0.0005 0.0018 0.0014 0.0029 0.0011 0.0008 0.0008 0.0012 0.0008 0.0022 0.0009 0.0003 0.0008
Endoplasmic Reticulum 0.0083 0.0085 0.0057 0.0036 0.0014 0.0048 0.0047 0.0023 0.0045 0.0035 0.0077 0.0026 0.0098 0.0033 0.0038 0.0023 0.0062 0.0079
Endosome 0.1552 0.1583 0.1154 0.1245 0.177 0.1274 0.1515 0.1308 0.0731 0.0636 0.1342 0.1224 0.183 0.1308 0.1247 0.0619 0.2094 0.1294
Golgi 0.0309 0.0284 0.0338 0.0407 0.0513 0.0387 0.059 0.0449 0.0391 0.0333 0.0591 0.0431 0.0752 0.071 0.0523 0.0517 0.0618 0.0583
Lipid Particles 0.0724 0.0294 0.0365 0.0526 0.0097 0.0384 0.0541 0.0176 0.0223 0.0106 0.0275 0.0301 0.0483 0.0185 0.0188 0.0033 0.038 0.0239
Mitochondria 0.2107 0.1846 0.2603 0.3019 0.3944 0.2401 0.183 0.1872 0.3099 0.1698 0.1228 0.1855 0.2353 0.2024 0.3269 0.4825 0.3665 0.2831
None 0.1701 0.2266 0.2438 0.2274 0.1281 0.2618 0.2122 0.2297 0.1703 0.2516 0.2897 0.1829 0.0949 0.1575 0.1428 0.1132 0.0689 0.1312
Nuclear Periphery 0.0194 0.0022 0.0057 0.0028 0.0006 0.003 0.0021 0.0005 0.0012 0.0005 0.0013 0.0008 0.0043 0.002 0.0008 0.0002 0.0017 0.002
Nucleolus 0.003 0.0035 0.0019 0.0011 0.0009 0.0016 0.0019 0.0027 0.0015 0.001 0.0007 0.0006 0.0014 0.0014 0.001 0.0006 0.0003 0.0005
Nucleus 0.0127 0.0063 0.0037 0.0023 0.0012 0.0019 0.0017 0.0022 0.0026 0.001 0.0011 0.0007 0.0021 0.0019 0.0012 0.0009 0.0006 0.0011
Peroxisomes 0.0462 0.0241 0.0391 0.0365 0.032 0.0563 0.0381 0.028 0.0728 0.07 0.0365 0.0817 0.0513 0.0259 0.055 0.0188 0.0518 0.0645
Punctate Nuclear 0.016 0.0047 0.0034 0.0018 0.0016 0.0028 0.0031 0.0018 0.0049 0.0025 0.0029 0.003 0.0041 0.0044 0.001 0.0002 0.0016 0.0017
Vacuole 0.0162 0.0252 0.0181 0.0105 0.0085 0.0105 0.0124 0.0108 0.0069 0.0065 0.007 0.0069 0.0149 0.0145 0.0085 0.0057 0.0102 0.0055
Vacuole Periphery 0.0058 0.0056 0.006 0.0065 0.005 0.0043 0.0031 0.0023 0.0024 0.0021 0.0029 0.003 0.0051 0.0033 0.0034 0.0019 0.0052 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3778 3.355 6.4729 8.1598 7.7856 6.7518 9.3287 11.9056 10.1586 10.2161
Translational Efficiency 0.947 0.7488 0.5037 0.5244 0.3878 0.5708 0.4475 0.4342 0.4686 0.4693

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘