| ORF | |
|---|---|
| Description | Potential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 3 | 17 | 13 | 6 | 4 | 1 | 3 | 1 | 4 | 4 | 1 | 1 | 3 | 0 | 0 | 31 | 25 | 24 | 12 | 18 | 53 | 20 | 23 | 32 |
| Bud | 0 | 8 | 9 | 7 | 5 | 15 | 25 | 12 | 17 | 14 | 12 | 11 | 1 | 0 | 2 | 3 | 8 | 8 | 6 | 11 | 12 | 17 | 11 | 22 |
| Bud Neck | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 3 | 0 | 5 | 8 | 16 | 18 | 17 | 18 |
| Bud Site | 0 | 0 | 1 | 0 | 2 | 8 | 2 | 2 | 1 | 5 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 1 | 5 | 3 | 3 | 1 | 5 | 4 | 0 | 2 | 2 | 2 | 1 | 1 | 1 | 8 | 15 | 12 | 0 | 2 | 6 | 2 | 4 | 5 |
| Cytoplasm | 96 | 173 | 205 | 141 | 156 | 255 | 293 | 189 | 192 | 186 | 122 | 148 | 261 | 214 | 282 | 130 | 187 | 152 | 47 | 127 | 93 | 82 | 108 | 116 |
| Endoplasmic Reticulum | 5 | 12 | 14 | 4 | 1 | 3 | 4 | 1 | 9 | 4 | 4 | 5 | 3 | 5 | 2 | 7 | 13 | 12 | 5 | 7 | 19 | 1 | 2 | 5 |
| Endosome | 1 | 2 | 5 | 1 | 5 | 4 | 1 | 4 | 1 | 0 | 2 | 0 | 3 | 1 | 4 | 3 | 8 | 17 | 8 | 10 | 13 | 5 | 5 | 4 |
| Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 1 | 1 | 1 |
| Mitochondria | 25 | 3 | 23 | 15 | 7 | 96 | 168 | 140 | 143 | 121 | 144 | 143 | 0 | 0 | 0 | 7 | 10 | 4 | 28 | 27 | 77 | 22 | 26 | 16 |
| Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
| SpindlePole | 0 | 2 | 3 | 4 | 5 | 5 | 6 | 9 | 3 | 2 | 1 | 2 | 4 | 0 | 3 | 7 | 9 | 7 | 0 | 2 | 2 | 4 | 2 | 1 |
| Vac/Vac Membrane | 0 | 1 | 1 | 0 | 3 | 0 | 2 | 6 | 3 | 2 | 4 | 1 | 1 | 2 | 4 | 7 | 7 | 12 | 9 | 16 | 11 | 10 | 10 | 10 |
| Unique Cell Count | 123 | 204 | 259 | 166 | 179 | 326 | 420 | 309 | 298 | 284 | 235 | 253 | 274 | 218 | 297 | 182 | 253 | 219 | 128 | 239 | 319 | 193 | 219 | 238 |
| Labelled Cell Count | 130 | 219 | 282 | 183 | 195 | 392 | 515 | 370 | 373 | 341 | 298 | 319 | 279 | 224 | 305 | 206 | 287 | 253 | 128 | 239 | 319 | 193 | 219 | 238 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.9 | 4.7 | 4.2 | 4.0 | 6.2 | 3.7 | 3.5 | 3.4 | 3.4 | 3.3 | 3.0 | 3.0 | 5.5 | 5.6 | 5.4 | 7.4 | 7.1 | 6.6 | 5.5 | 5.5 | 5.9 |
| Std Deviation (1e-4) | 0.7 | 1.0 | 0.9 | 1.5 | 6.6 | 1.6 | 1.4 | 1.3 | 1.3 | 0.8 | 1.1 | 0.8 | 1.1 | 1.1 | 1.5 | 1.0 | 1.3 | 1.1 | 1.3 | 1.6 | 1.7 |
| Intensity Change (Log2) | – | – | – | -0.07 | 0.56 | -0.17 | -0.27 | -0.29 | -0.29 | -0.34 | -0.5 | -0.48 | 0.4 | 0.41 | 0.37 | 0.82 | 0.76 | 0.65 | 0.39 | 0.4 | 0.49 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 3.4 |
External localization resources
