Standard name
Human Ortholog
Description Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.24 0.15 0.17 0.12 0.07 0 0 0 0 0 0.08 0 0.07 0 0 0.11 0.06 0.09 0.07 0.07 0.06 0.06
Bud 0.07 0 0 0.06 0 0 0 0.05 0.06 0.06 0.05 0.06 0 0.05 0 0.15 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.54 0.68 0.6 0.5 0.38 0.31 0.32 0.2 0.27 0.3 0.31 0.34 0.45 0.46 0.52 0.5 0.71 0.72 0.8 0.72 0.75 0.74
Cytoplasm 0.38 0.41 0.37 0.45 0.53 0.56 0.52 0.51 0.49 0.48 0.37 0.4 0.73 0.75 0.74 0.46 0.06 0.05 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.05 0.2 0.31 0.17 0.41 0.45 0.46 0.54 0.56 0.6 0.56 0 0 0 0.15 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.12 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 51 12 53 33 19 9 5 20 11 19 38 11 21 9 12 8 14 10 25 22 36 36
Bud 14 2 12 18 11 20 20 36 28 32 21 29 5 14 5 11 1 1 2 1 4 3
Bud Neck 2 0 1 0 0 0 0 1 0 0 0 0 6 1 5 1 8 9 4 15 23 30
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Cell Periphery 113 54 187 143 99 142 187 139 122 167 142 152 143 141 173 36 186 86 295 243 433 425
Cytoplasm 80 33 116 129 138 258 308 358 224 265 171 179 232 230 247 33 16 5 8 19 19 19
Endoplasmic Reticulum 4 2 2 1 2 5 6 5 4 3 18 5 2 3 3 4 15 0 14 6 19 17
Endosome 1 0 2 0 4 3 3 3 1 0 0 0 0 1 0 0 4 0 1 2 3 3
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 6 0
Mitochondria 20 4 63 89 44 191 264 321 245 309 274 253 0 2 0 11 5 1 9 19 19 18
Nucleus 1 0 0 0 1 0 2 2 1 2 3 1 0 2 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 2 0 1 3 2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 35 33 20 22 19 20 6 5 5 9 0 0 0 0 0 0 1 0 0 0
SpindlePole 4 0 7 5 5 3 7 10 3 1 2 6 2 1 0 2 0 0 1 0 0 0
Vac/Vac Membrane 0 0 1 6 3 7 2 7 3 2 0 1 0 0 0 0 3 2 0 2 6 8
Unique Cell Count 210 80 311 285 260 462 589 700 457 551 460 450 319 308 334 72 263 119 369 337 579 572
Labelled Cell Count 291 108 479 458 346 660 825 925 648 806 678 649 411 404 445 106 263 119 369 337 579 572


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 6.2 6.0 5.8 5.6 4.7 4.6 4.8 4.7 4.4 4.6 4.5 6.3 6.5 6.8 4.5 4.7 5.7 6.6 6.8 6.8
Std Deviation (1e-4) 0.9 1.0 1.7 1.7 1.6 1.3 1.4 1.3 1.3 0.9 1.2 1.0 0.8 0.8 0.8 0.8 0.8 0.9 1.1 1.9 1.4
Intensity Change (Log2) -0.05 -0.09 -0.33 -0.37 -0.3 -0.34 -0.45 -0.36 -0.4 0.09 0.12 0.19 -0.42 -0.34 -0.07 0.14 0.2 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.9 -3.5 0 0 0 0 0 0 0 -4.1 -5.9 -5.7 -1.2
Bud 1.4 0.2 0 0 0 0 0 0 0 -1.8 0.4 -1.9 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.4 -5.2 0 0 0 0 0 0 0 -3.8 -3.6 -2.1 -1.6
Cytoplasm 2.0 3.8 5.1 4.3 4.1 3.2 3.1 0 0.7 8.9 9.4 9.4 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 -1.0 0 0 0 0 0 0 0 -8.5 -8.0 -8.7 -1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 -1.4 0 0 0 0 0 0 0 -6.2 -6.1 -6.3 -3.0
SpindlePole -0.4 -0.3 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5925 2.8208 2.9613 2.2617 2.4351 2.5165 2.5706 3.6513 3.0752 3.1652 3.8328 3.5082 3.1913 3.5449 4.2997 3.8103 3.5199 4.3976
Actin 0.061 0.0056 0.0058 0.0337 0.0083 0.0016 0.07 0.0058 0.0531 0.0101 0.0004 0.0266 0.0002 0.0002 0.0256 0.0004 0.0031 0.0014
Bud 0.0016 0.0002 0.0004 0.0015 0.0019 0.0003 0.0024 0.0003 0.001 0.0005 0.0001 0.0018 0.0001 0.0001 0.0004 0.0008 0.0003 0.0019
Bud Neck 0.0049 0.0032 0.0052 0.0385 0.0031 0.0033 0.0075 0.0052 0.0047 0.005 0.0016 0.0147 0.0011 0.0014 0.0062 0.002 0.0028 0.0091
Bud Periphery 0.0078 0.0016 0.0036 0.0104 0.0067 0.0061 0.0132 0.0015 0.0057 0.0091 0.002 0.0093 0.0011 0.0005 0.0025 0.0224 0.0038 0.0114
Bud Site 0.0153 0.0439 0.0402 0.0321 0.0057 0.0009 0.0758 0.1331 0.0138 0.0034 0.0007 0.0119 0.0099 0.0604 0.013 0.0015 0.0024 0.0041
Cell Periphery 0.3035 0.3082 0.2932 0.2554 0.2885 0.3881 0.3715 0.2799 0.3715 0.4974 0.3783 0.3737 0.4248 0.5083 0.3819 0.8287 0.4115 0.4847
Cytoplasm 0.3026 0.5544 0.4841 0.4567 0.2667 0.5065 0.3085 0.4697 0.4453 0.3636 0.4342 0.416 0.491 0.4186 0.437 0.1397 0.4137 0.3936
Cytoplasmic Foci 0.039 0.0015 0.0044 0.0234 0.0639 0.0024 0.0033 0.0017 0.0049 0.0006 0.0008 0.0117 0.0018 0.0001 0.0017 0 0.0105 0.0012
Eisosomes 0.0038 0.0035 0.0031 0.0015 0.0009 0.0011 0.0073 0.0038 0.0015 0.0019 0.0003 0.0024 0.001 0.0012 0.0032 0.0001 0.0006 0.0004
Endoplasmic Reticulum 0.0138 0.0089 0.008 0.0053 0.0108 0.0124 0.0184 0.0037 0.004 0.0066 0.0033 0.0055 0.0073 0.006 0.0056 0.0014 0.0109 0.0023
Endosome 0.0366 0.0002 0.0007 0.0277 0.1494 0.0019 0.0036 0.0001 0.0061 0 0.0003 0.0029 0.0001 0 0.0067 0 0.0311 0.0018
Golgi 0.017 0.001 0.002 0.005 0.0873 0.002 0.0046 0.0003 0.0081 0.0003 0.0007 0.0049 0.0001 0.0001 0.0016 0 0.0289 0.0005
Lipid Particles 0.0259 0.0016 0.0027 0.0038 0.0248 0.0025 0.0039 0.001 0.0024 0.0007 0.0032 0.0066 0.0039 0.0004 0.0134 0.0002 0.0141 0.0015
Mitochondria 0.0223 0.0001 0.0004 0.0013 0.0142 0.0092 0.002 0 0.0184 0 0.0754 0.0157 0 0 0.0009 0 0.0073 0.0002
None 0.0875 0.0631 0.1338 0.0829 0.0017 0.0501 0.0986 0.0912 0.0539 0.0988 0.0928 0.0829 0.0503 0.0017 0.0537 0.0002 0.0513 0.0686
Nuclear Periphery 0.0174 0.0003 0.0002 0.0007 0.0022 0.0012 0.0014 0.0002 0.0003 0.0002 0.0001 0.0003 0.0003 0.0003 0.0101 0.0001 0.0004 0.0002
Nucleolus 0.001 0.0001 0.0002 0.0005 0.0004 0.0001 0.0005 0.0002 0.0002 0.0001 0.0001 0.0004 0.0003 0 0.0009 0 0.0001 0.0003
Nucleus 0.003 0.0003 0.0003 0.0014 0.0021 0.0021 0.0008 0.0004 0.0003 0.0002 0.0001 0.0004 0.0003 0.0001 0.0183 0.0001 0.0003 0.0003
Peroxisomes 0.0151 0 0.0094 0.0035 0.026 0.0047 0.0008 0.0001 0.0016 0 0.0036 0.0076 0 0 0.0006 0 0.0028 0.0001
Punctate Nuclear 0.0095 0.0001 0.0002 0.0035 0.0008 0.0003 0.0021 0.0001 0.0002 0 0 0.0007 0.0001 0 0.0004 0 0.0002 0.0001
Vacuole 0.0095 0.0023 0.0021 0.0105 0.0314 0.0027 0.0033 0.0015 0.0026 0.0014 0.0018 0.0038 0.0062 0.0006 0.0146 0.0022 0.0032 0.0161
Vacuole Periphery 0.0019 0 0 0.0007 0.0035 0.0001 0.0002 0 0.0005 0 0.0001 0.0002 0 0 0.0015 0 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4714 17.3638 25.7694 27.4989 23.5592 17.5345 29.1665 33.8283 29.2307 26.2832
Translational Efficiency 0.5299 0.6534 0.4599 0.4714 0.4189 0.3811 0.3929 0.311 0.3072 0.306

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 653 471 1091 1192 1749 2909 1643 1281 2402 3380 2734

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 955.56 722.99 1137.87 997.16 736.34 871.08 1017.24 1021.49 751.57 830.82 1034.05 1011.78
Standard Deviation 165.37 130.38 135.25 135.19 97.08 110.51 118.33 132.84 117.38 133.62 127.85 134.31
Intensity Change Log 2 -0.402371 0.251917 0.061479 0.242433 0.466216 0.472231 -0.085929 0.349117 0.254746

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009788 0.017823 0.034391 0.052246 0.007691 0.009434 0.019569 0.052453 0.007837 0.011715 0.021635 0.052370
Bud Neck 0.011818 0.058350 0.023009 0.037943 0.051340 0.053402 0.056363 0.037434 0.048594 0.054747 0.051715 0.037637
Bud Site 0.054860 0.117215* 0.197955* 0.206364* 0.046561 0.034404 0.085801 0.145765* 0.047138 0.056917 0.101430* 0.169947*
Cell Periphery 0.018253 0.099484 0.441287* 0.399383* 0.127510* 0.146040* 0.456768* 0.393635* 0.119919* 0.133384* 0.454611* 0.395929*
Cytoplasm 0.271352* 0.392876* 0.118713* 0.197475* 0.541266* 0.562925* 0.257016* 0.256378* 0.522513* 0.516696* 0.237743* 0.232873*
Cytoplasmic Foci 0.060053 0.107078 0.009260 0.003952 0.032375 0.031260 0.001964 0.004110 0.034298 0.051872 0.002980 0.004047
Eisosomes 0.002700 0.011688 0.011812 0.035469 0.040456 0.061589 0.038066 0.044400 0.037832 0.048023 0.034407 0.040836
Endoplasmic Reticulum 0.015482 0.017168 0.094480 0.013859 0.023807 0.005264 0.017855 0.006515 0.023229 0.008500 0.028532 0.009446
Endosome 0.035144 0.035477 0.002054 0.001514 0.002604 0.003761 0.000891 0.002078 0.004865 0.012383 0.001053 0.001853
Golgi 0.023695 0.019379 0.002279 0.001165 0.007335 0.012090 0.001170 0.003937 0.008471 0.014072 0.001325 0.002831
Lipid Particles 0.002265 0.040115 0.004884 0.001974 0.008747 0.008737 0.002043 0.001740 0.008296 0.017268 0.002439 0.001833
Mitochondria 0.011752 0.007939 0.000649 0.001661 0.007301 0.004131 0.002365 0.003014 0.007610 0.005167 0.002126 0.002474
Mitotic Spindle 0.018321 0.000154 0.000505 0.000526 0.000090 0.000184 0.000562 0.000390 0.001357 0.000176 0.000554 0.000444
None 0.000149 0.003163 0.000106 0.000145 0.000894 0.001216 0.000617 0.000132 0.000842 0.001745 0.000546 0.000138
Nuclear Periphery 0.000403 0.000401 0.000309 0.000132 0.000166 0.000108 0.000304 0.000117 0.000182 0.000188 0.000305 0.000123
Nuclear Periphery Foci 0.001539 0.001262 0.002885 0.000817 0.000433 0.000327 0.000435 0.000507 0.000510 0.000581 0.000777 0.000630
Nucleolus 0.000075 0.000449 0.000035 0.000025 0.000123 0.000141 0.000076 0.000028 0.000120 0.000225 0.000070 0.000027
Nucleus 0.084535 0.019263 0.021337 0.010174 0.073900 0.032805 0.034087 0.012295 0.074639 0.029124 0.032311 0.011449
Peroxisomes 0.003090 0.010950 0.000895 0.001027 0.007573 0.008400 0.002266 0.001462 0.007261 0.009094 0.002075 0.001288
Vacuole 0.366508* 0.037022 0.032874 0.033699 0.019324 0.023415 0.021584 0.032401 0.043445 0.027114 0.023157 0.032919
Vacuole Periphery 0.008218 0.002743 0.000279 0.000449 0.000506 0.000364 0.000197 0.001209 0.001042 0.001011 0.000208 0.000906

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.81 -6.97 -10.98 -9.47 -4.69 -2.08 -12.14 -17.59 -16.82 -12.51 -4.22 -14.53 -22.83 -20.06 -15.03
Bud Neck -10.95 -6.28 -11.79 4.43 -5.99 -0.64 -1.62 4.78 6.03 7.78 -2.10 -1.15 4.19 7.54 7.02
Bud Site -5.50 -10.23 -12.27 -9.23 -0.76 3.76 -10.97 -18.10 -21.19 -10.80 -2.99 -15.14 -26.25 -23.94 -14.14
Cell Periphery -7.35 -25.05* -30.62* -23.45* 2.23 -2.34 -39.95* -26.58 -25.89* 6.59 -1.92 -43.15 -32.97 -34.54* 7.33
Cytoplasm -5.37 4.19 1.41 16.62 -8.09 -2.56 36.63 33.11 39.18 -0.01 0.43 36.52 36.22 43.10 0.74
Cytoplasmic Foci -5.58 5.79 6.67 17.56 3.67 0.46 16.59 14.82 15.26 -3.59 -6.52 17.12 16.38 22.13 -2.07
Eisosomes -6.51 -7.87 -15.26 -10.05 -10.66 -7.58 1.10 -1.36 6.22 -2.74 -4.26 1.65 -1.24 3.48 -3.62
Endoplasmic Reticulum -0.90 -9.77 -0.33 1.23 11.50 9.25 2.60 8.55 -1.83 10.84 7.37 -2.60 6.93 -1.12 13.16
Endosome -1.28 3.46 3.54 11.90 1.09 -2.79 6.72 1.13 2.99 -2.54 -8.06 5.71 3.79 11.48 -2.24
Golgi -0.38 1.82 1.96 12.24 3.30 -5.39 10.95 3.30 6.70 -2.71 -6.34 8.97 5.81 12.47 -2.26
Lipid Particles -8.85 -3.83 0.67 8.94 4.71 0.01 9.92 9.87 10.34 0.83 -6.40 9.23 10.14 11.97 2.20
Mitochondria 0.05 1.94 1.65 5.16 -1.39 3.27 6.09 4.19 1.33 -0.92 2.38 6.56 5.31 3.94 -0.65
Mitotic Spindle 1.91 1.87 1.86 -2.16 -0.19 -2.13 -4.37 -3.33 -2.05 1.49 1.75 1.05 1.29 -2.88 1.10
None -2.54 1.04 0.07 2.55 -0.93 -0.73 1.54 4.73 2.62 5.94 -1.96 1.80 4.69 3.65 5.72
Nuclear Periphery 1.03 2.17 3.67 2.50 5.93 7.27 -10.85 2.22 -0.47 8.18 0.13 -7.64 3.91 2.00 10.56
Nuclear Periphery Foci 0.92 -1.74 1.78 2.34 5.87 3.05 -0.26 -1.68 -4.04 -1.52 -0.89 -3.62 -1.68 -0.75 2.07
Nucleolus -4.27 3.26 4.00 4.99 3.55 -0.75 5.84 12.84 4.34 12.71 -3.50 6.70 13.60 6.60 13.84
Nucleus 3.72 3.55 4.29 4.26 5.28 11.39 10.70 18.03 10.95 12.47 13.03 11.64 18.96 12.22 14.41
Peroxisomes -7.07 1.82 1.64 10.48 -0.90 -1.12 9.00 10.63 12.10 2.87 -2.73 9.37 11.23 16.01 3.54
Vacuole 11.80 11.83 11.87 0.55 0.03 -2.57 -2.30 -6.77 -5.07 -5.88 5.35 6.14 3.40 -4.34 -6.59
Vacuole Periphery 1.28 2.36 2.28 6.61 -0.94 3.02 6.97 -1.62 -1.95 -2.34 -0.75 3.91 -0.10 0.39 -2.59