Standard name
Human Ortholog
Description Putative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.09 0.06 0 0.05 0 0 0
Cytoplasm 0.93 0.95 0.91 0.93 0.9 0.89 0.78 0.77 0.65 0.61 0.56 0.56 0.99 0.99 0.99 0.69 0.68 0.67 0.71 0.76 0.35 0.75 0.79 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.16 0.25 0.26 0.46 0.56 0.58 0.53 0 0 0 0.14 0.05 0.07 0.09 0.1 0.2 0.16 0.09 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.21 0.15 0 0 0.17 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 3 3 2 0 2 2 0 0 0 1 0 1 1 2 1 4 6 3 16 2 4 4
Bud 5 3 5 4 0 5 2 15 17 12 24 10 0 1 0 1 2 2 1 5 9 0 1 6
Bud Neck 1 0 1 1 0 1 0 3 2 1 1 1 12 13 8 0 1 0 4 2 7 1 0 6
Bud Site 0 0 0 1 0 1 0 1 1 3 0 0 0 0 1 1 0 0
Cell Periphery 5 2 10 11 4 7 4 1 1 2 4 5 17 9 6 21 5 10 9 5 13 2 2 1
Cytoplasm 109 208 249 240 221 383 365 527 298 317 281 287 441 467 475 122 56 72 99 166 96 101 141 140
Endoplasmic Reticulum 1 1 4 0 0 0 0 3 1 0 1 1 3 3 2 4 7 6 1 1 10 0 0 0
Endosome 1 0 2 2 5 5 10 14 7 4 2 6 0 0 0 4 3 1 1 1 7 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 0
Mitochondria 3 11 7 7 6 71 117 177 210 291 287 272 0 0 0 25 4 8 13 22 55 21 16 15
Nucleus 0 0 0 1 0 2 2 5 0 1 2 1 1 1 1 10 3 6 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 1 1 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 0 1 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 1 2 5 2 9 6 0 1 4 6 0 0 0 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 7 17 16 17 9 12 19 0 0 1 0 1 0 1 2 2 0 2 3
Vac/Vac Membrane 0 0 6 1 10 9 10 8 11 4 4 12 0 0 0 32 17 16 1 5 45 2 5 6
Unique Cell Count 117 220 275 257 245 432 469 686 455 521 499 512 446 472 479 178 82 108 141 221 272 135 179 192
Labelled Cell Count 128 229 290 275 254 494 539 780 566 646 625 624 475 495 495 223 101 126 141 221 272 135 179 192


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.6 4.4 4.3 4.0 3.4 3.0 3.4 3.0 2.6 2.9 2.9 4.5 4.9 4.9 6.3 6.1 6.7 4.7 4.9 5.2
Std Deviation (1e-4) 2.1 1.7 2.6 2.4 1.9 2.0 1.3 1.9 1.3 0.8 1.6 0.8 0.7 1.1 0.8 5.8 5.9 5.3 0.7 1.2 1.3
Intensity Change (Log2) -0.04 -0.14 -0.38 -0.57 -0.38 -0.59 -0.79 -0.63 -0.63 0.03 0.13 0.15 0.49 0.47 0.6 0.08 0.14 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 5.9 6.0 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2571 0.2598 0.0353 0.2372 -0.2257 0.5599 1.9661 2.6208 1.0338 0.7779 0.1134 1.518 0.3694 0.3212 0.3852 0.9224 0.0218 0.6187
Actin 0.0337 0.0006 0.0056 0.007 0.0011 0.0229 0.1338 0.0014 0.0005 0.0001 0.0007 0.0065 0.0674 0.0002 0.0028 0.0372 0 0.0025
Bud 0.0012 0.0075 0.0012 0.0015 0.0011 0.0019 0.0059 0.0085 0.0004 0 0.0217 0.0005 0.0002 0.0007 0.0012 0.002 0 0.0016
Bud Neck 0.0074 0.0003 0.0022 0.0006 0.0003 0.017 0.0014 0.0008 0.0024 0.0001 0.0013 0.0019 0.0002 0.0001 0.0002 0.0016 0 0.0004
Bud Periphery 0.0069 0.012 0.01 0.0021 0.0019 0.0113 0.0381 0.0625 0.0008 0 0.0345 0.004 0.0005 0.0015 0.0019 0.0043 0.0001 0.0143
Bud Site 0.0064 0.0061 0.006 0.0013 0.0006 0.0017 0.0139 0.0339 0.014 0.0001 0.0042 0.0017 0.0004 0.0156 0.0102 0.0209 0 0.0008
Cell Periphery 0.0041 0.0089 0.0013 0.0012 0.0003 0.0037 0.0153 0.068 0.0061 0.0001 0.0011 0.0381 0.0015 0.0011 0.0015 0.0026 0.0001 0.0104
Cytoplasm 0.0294 0.047 0.0523 0.0789 0.0323 0.0668 0.0858 0.0976 0.085 0.1164 0.2706 0.1218 0.146 0.1576 0.0924 0.1566 0.1078 0.1394
Cytoplasmic Foci 0.0192 0.0106 0.0162 0.0222 0.0084 0.0243 0.0373 0.0045 0.0107 0.0024 0.0422 0.004 0.0289 0.0116 0.0258 0.0086 0.0196 0.0199
Eisosomes 0.0011 0.0002 0.0004 0.0002 0.0007 0.0003 0.0048 0.0011 0.0005 0.0001 0.0001 0.0002 0.0012 0.0001 0.0004 0.0012 0.0001 0.0001
Endoplasmic Reticulum 0.0075 0.0013 0.0013 0.0032 0.0009 0.001 0.0526 0.0017 0.0007 0.0005 0.0427 0.0011 0.0083 0.0035 0.0041 0.0169 0.0013 0.0022
Endosome 0.0258 0.0137 0.0089 0.0431 0.0195 0.0214 0.0386 0.0046 0.019 0.0031 0.1021 0.0101 0.0246 0.0078 0.0446 0.0104 0.0373 0.0157
Golgi 0.0047 0.0005 0.0003 0.0042 0.0009 0.002 0.0176 0.0002 0.0009 0 0.0016 0.001 0.0099 0.0003 0.0011 0.0011 0.0003 0.0006
Lipid Particles 0.0078 0.0023 0.0013 0.0073 0.0028 0.0041 0.0485 0.0006 0.003 0.0001 0.0287 0.0033 0.0324 0.0013 0.0028 0.0018 0.0008 0.0064
Mitochondria 0.0095 0.0014 0.0016 0.0027 0.001 0.0005 0.0031 0.0005 0.0017 0.0001 0.0028 0.0069 0.0041 0.0003 0.0035 0.0078 0.0005 0.0003
None 0.7675 0.8456 0.8694 0.7102 0.8401 0.7986 0.398 0.6959 0.7977 0.87 0.4158 0.6949 0.6296 0.7918 0.7543 0.7119 0.8085 0.7507
Nuclear Periphery 0.0137 0.0008 0.0006 0.0098 0.0039 0.0026 0.0029 0.0004 0.0006 0.0003 0.0045 0.0004 0.0031 0.0007 0.0017 0.0024 0.0008 0.0005
Nucleolus 0.0009 0.0009 0.0004 0.0012 0.0016 0.0003 0.0005 0.0003 0.0012 0.0001 0.0005 0.0002 0.0003 0.0003 0.0004 0.0004 0.0002 0.0006
Nucleus 0.0035 0.0018 0.0008 0.0039 0.0041 0.0008 0.0008 0.0006 0.0016 0.0009 0.0013 0.0006 0.001 0.0005 0.001 0.0014 0.0006 0.0006
Peroxisomes 0.0092 0.0004 0.0005 0.0018 0.0005 0.0013 0.0397 0.0001 0.0007 0 0.0007 0.012 0.0257 0.0001 0.0004 0.0004 0.0002 0.0019
Punctate Nuclear 0.0222 0.0014 0.0004 0.0475 0.0404 0.0028 0.0029 0.0001 0.0004 0.0001 0.0009 0.0002 0.0024 0.0006 0.0007 0.0006 0.0004 0.0017
Vacuole 0.0137 0.033 0.0175 0.0432 0.0318 0.0144 0.0568 0.0156 0.0505 0.0051 0.0197 0.0814 0.0116 0.0039 0.0451 0.0088 0.0197 0.0287
Vacuole Periphery 0.0045 0.0037 0.0015 0.007 0.0059 0.0005 0.0018 0.001 0.0018 0.0004 0.0023 0.0091 0.0007 0.0003 0.0037 0.001 0.0017 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
103 216 157 1099 610 1622 1774 1187 713 1838 1931 2286

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1410.88 758.06 5413.48 819.35 634.90 711.97 940.66 829.62 747.00 717.39 1304.32 824.68
Standard Deviation 490.96 118.52 1166.77 125.45 70.22 93.89 233.07 128.41 336.84 98.24 1286.44 127.10
Intensity Change Log 2 -0.896211 1.939961 -0.784044 0.165287 0.567144 0.385921 -0.476805 1.635046 -0.311086

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.010662 0.007586 0.000850 0.005869 0.000308 0.003022 0.042760 0.015698 0.001803 0.003558 0.039352 0.010973
Bud Neck 0.014075 0.043815 0.004164 0.018086 0.032114 0.048930 0.083295 0.015574 0.029508 0.048329 0.076861 0.016782
Bud Site 0.177244* 0.087130 0.000679 0.065003 0.005971 0.037434 0.325500* 0.074995 0.030713 0.043274 0.299090* 0.070191
Cell Periphery 0.250330* 0.023906 0.651483* 0.004924 0.000824 0.006541 0.087953 0.012506 0.036868 0.008582 0.133771* 0.008861
Cytoplasm 0.065263 0.317522* 0.000009 0.390841* 0.335638* 0.376117* 0.207221* 0.384336* 0.296579* 0.369231* 0.190374* 0.387463*
Cytoplasmic Foci 0.027922 0.257881* 0.000064 0.016837 0.377247* 0.290490* 0.010928 0.039539 0.326783* 0.286658* 0.010045 0.028625
Eisosomes 0.001514 0.003758 0.218229* 0.001099 0.000668 0.001682 0.004366 0.000841 0.000791 0.001926 0.021754 0.000965
Endoplasmic Reticulum 0.027209 0.000844 0.005247 0.002452 0.000612 0.000769 0.020903 0.002204 0.004455 0.000778 0.019630 0.002323
Endosome 0.025764 0.029909 0.000971 0.005303 0.018676 0.024021 0.001958 0.015840 0.019700 0.024713 0.001878 0.010774
Golgi 0.013408 0.003789 0.003956 0.005473 0.002901 0.007397 0.000411 0.006219 0.004419 0.006973 0.000700 0.005861
Lipid Particles 0.006480 0.007166 0.003787 0.001370 0.018960 0.009618 0.002068 0.002543 0.017157 0.009330 0.002208 0.001979
Mitochondria 0.007364 0.005084 0.000417 0.002698 0.010605 0.015481 0.001017 0.014034 0.010137 0.014259 0.000968 0.008584
Mitotic Spindle 0.007160 0.001068 0.002554 0.012521 0.000474 0.003727 0.001047 0.044846 0.001440 0.003414 0.001169 0.029306
None 0.000077 0.001556 0.000082 0.004854 0.001428 0.002248 0.001691 0.002828 0.001233 0.002166 0.001560 0.003802
Nuclear Periphery 0.000350 0.000266 0.008106 0.001404 0.000526 0.000242 0.000462 0.001090 0.000500 0.000245 0.001083 0.001241
Nuclear Periphery Foci 0.001660 0.000548 0.000181 0.000814 0.000175 0.000371 0.001080 0.000868 0.000389 0.000392 0.001007 0.000842
Nucleolus 0.000139 0.001638 0.000094 0.000363 0.000911 0.001371 0.000130 0.000942 0.000799 0.001403 0.000128 0.000664
Nucleus 0.020934 0.087981 0.000242 0.341702* 0.125529 0.093864 0.142515* 0.212012* 0.110419 0.093173 0.130947* 0.274361*
Peroxisomes 0.001419 0.027811 0.000006 0.002733 0.025274 0.022213 0.001250 0.006765 0.021828 0.022871 0.001149 0.004826
Vacuole 0.321724* 0.089432 0.096427 0.114753 0.040165 0.052751 0.063254 0.141379 0.080839 0.057061 0.065951 0.128578
Vacuole Periphery 0.019299 0.001309 0.002453 0.000900 0.000997 0.001712 0.000192 0.004941 0.003641 0.001665 0.000375 0.002998

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.57 1.80 1.47 2.69 -1.90 -4.34 -15.12 -4.70 -2.75 10.27 -1.79 -13.63 -2.89 -1.58 12.03
Bud Neck -4.20 3.38 0.94 4.98 -4.06 -4.11 -8.95 6.36 13.12 17.48 -5.10 -9.20 6.41 14.11 17.30
Bud Site 3.18 6.64 5.50 3.75 -8.14 -8.95 -34.51* -8.15 -0.08 28.24* -2.07 -26.15 -0.58 2.08 28.66*
Cell Periphery 6.78 -10.79 7.63 2.74 29.35* -3.40 -16.71 -2.70 0.58 14.99 4.71 -10.91 5.70 2.57 20.51
Cytoplasm -11.65 5.72 -16.27 0.41 -39.41 -3.52 10.45 -0.17 3.72 -11.97 -6.54 9.11 -2.47 5.43 -15.52
Cytoplasmic Foci -12.70 3.11 1.64 15.45 -13.61 7.95 39.50 36.99 41.97 -8.78 3.72 34.16 32.87 46.77 -8.21
Eisosomes -1.42 -10.09 1.23 2.44 10.14 -2.47 -8.23 1.30 3.31 9.64 -2.76 -9.18 1.50 3.80 9.35
Endoplasmic Reticulum 2.48 2.04 2.19 -9.38 1.39 -0.55 -10.03 -9.31 -6.63 8.77 2.30 -6.14 0.61 -9.47 8.65
Endosome -0.60 4.38 3.98 5.54 -4.61 -2.35 8.98 5.02 9.23 -5.45 -2.29 9.95 7.49 13.02 -5.95
Golgi 1.87 1.84 2.26 1.40 1.82 -6.18 7.03 0.45 5.49 -3.70 -2.72 4.80 2.26 6.10 -3.08
Lipid Particles -0.33 0.74 1.70 4.85 0.91 4.18 7.84 7.86 10.76 0.11 3.95 7.84 8.12 12.15 1.53
Mitochondria 0.38 1.24 1.06 1.71 -3.14 -1.85 4.58 1.70 4.24 -3.65 -1.71 4.68 2.95 6.26 -3.86
Mitotic Spindle 1.51 1.25 -0.44 -4.68 -4.25 -3.26 -2.31 -7.77 -6.56 -7.50 -1.84 0.12 -8.37 -7.10 -8.73
None -2.49 -0.05 -13.73 -3.38 -13.75 -1.59 -0.75 -3.55 -1.79 -3.17 -2.08 -1.00 -6.28 -3.35 -5.73
Nuclear Periphery 0.69 -10.77 -15.78 -19.75 7.60 2.26 -1.54 -10.77 -16.54 -13.20 2.33 -6.34 -15.36 -23.93 -9.60
Nuclear Periphery Foci 1.52 3.06 0.71 -1.59 -9.46 -1.19 -6.94 -6.74 -3.49 0.78 -0.14 -4.74 -6.74 -4.63 -0.32
Nucleolus -2.46 1.06 -3.30 2.20 -6.77 -1.48 5.00 1.31 2.59 -4.16 -2.37 5.01 1.97 3.95 -5.53
Nucleus -4.34 2.08 -24.19 -24.96 -45.22* 4.61 -6.73 -15.25 -21.89 -9.18 2.75 -7.15 -26.85 -36.07 -19.47
Peroxisomes -4.99 2.38 0.69 5.13 -3.24 0.97 8.34 7.48 11.23 -2.72 -0.31 8.33 7.82 13.35 -2.51
Vacuole 7.36 7.38 3.59 -9.39 -10.12 -3.70 -10.53 -24.86 -23.57 -18.48 3.62 -0.71 -17.94 -28.95 -22.55
Vacuole Periphery 2.78 2.60 2.93 2.79 6.79 -3.30 6.80 -2.37 -0.85 -4.14 1.99 3.40 2.33 1.20 -3.63