ORF
Human Ortholog
Description Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.3 0.43 0.58 0.37 0.74 0.77 0.83 – 0.8 0.8 0.85 0.42 0.8 0.96 0.25 0.32 0.23 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0.12 0 0.15 0.14 0.15 0.18 – 0.21 0.21 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.13 0.08 0 0.12 0.12 0.08 0 0 – 0 0 0 0.07 0 0 0.3 0.17 0.28 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.8 0.58 0.66 0.69 0.32 0.2 0.16 – 0.2 0.15 0.13 0.58 0.26 0.06 0.6 0.64 0.55 0.87 0.91 0.87 0.84 0.81 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 3 5 3 – 2 8 5 0 0 0 0 0 0 1 1 3 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 1 0 0 0 1 2 1 0 0 0 0 0 0 0 0
Cytoplasm 43 116 143 149 111 337 355 393 – 395 377 438 103 205 325 46 41 28 4 8 16 5 6 2
Endoplasmic Reticulum 0 2 1 0 0 0 1 0 – 3 0 0 3 5 2 9 1 2 0 0 1 0 3 2
Endosome 6 6 21 1 3 0 0 3 – 1 0 1 12 1 0 5 1 5 8 12 9 4 7 6
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 1 3 1 0 0 1 2
Mitochondria 20 13 40 11 46 62 69 84 – 103 100 112 2 0 1 4 3 4 5 5 7 2 6 5
Nucleus 35 31 6 30 35 35 20 9 – 3 6 7 17 7 5 55 22 35 6 4 1 2 1 1
Nuclear Periphery 2 0 0 5 5 0 20 2 – 5 2 2 0 0 0 0 0 0 3 0 0 1 3 2
Nucleolus 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 5 0 0 1 1 0 – 0 0 1 0 0 0 0 0 1 1 0 0 2 1 0
Vac/Vac Membrane 209 314 190 170 206 144 92 75 – 99 72 68 141 67 22 108 83 68 261 368 302 150 159 157
Unique Cell Count 268 393 330 259 300 457 461 475 496 470 517 243 257 340 181 129 123 299 407 349 178 197 189
Labelled Cell Count 315 482 407 366 407 583 563 569 612 565 634 279 286 357 230 155 144 299 407 349 178 197 189


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.3 4.6 4.6 4.4 4.0 3.6 3.7 – 3.5 3.6 3.6 5.6 4.9 4.9 6.5 6.6 6.5 6.7 6.6 6.7
Std Deviation (1e-4) 1.1 1.0 1.4 1.3 1.3 1.0 1.1 1.2 – 1.0 1.1 1.0 1.0 0.8 0.8 1.4 1.5 1.7 1.4 1.8 1.5
Intensity Change (Log2) – – – 0.01 -0.05 -0.2 -0.35 -0.28 – -0.4 -0.35 -0.35 0.3 0.12 0.12 0.51 0.52 0.52 0.55 0.53 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 3.4 -1.6 8.6 9.7 11.7 – 10.7 10.8 12.7 -0.2 8.9 14.7 -4.0 -2.3 -4.0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 -0.7 -3.8 -4.7 -1.8 -2.5 -0.9
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -5.1 -5.8 -6.4 -3.8 -3.2 -2.8
Nucleus 4.9 5.0 3.6 2.0 0.1 – 0 0 -0.5 3.1 0.7 -0.4 9.5 6.1 8.8
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 2.0 2.9 -7.3 -10.9 -12.4 – -11.1 -12.5 -13.7 0.1 -7.6 -14.2 0.5 1.3 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3221 1.8139 1.2542 0.8722 0.8937 1.1128 2.5978 3.5457 3.4161 3.1666 2.7231 3.0276 0.0474 1.6618 0.7784 0.9613 1.0794 1.0344
Actin 0.0056 0 0.0001 0 0 0 0.0001 0 0.016 0.0346 0 0 0 0.0001 0 0 0 0
Bud 0.0004 0.0006 0.0004 0.0002 0.0005 0.0004 0.0003 0.0004 0.0004 0.0006 0.0002 0.0002 0.0002 0.0011 0.0003 0.0002 0.0002 0.0004
Bud Neck 0.0039 0.0013 0.0009 0.0017 0.0012 0.0013 0.0075 0.0009 0.013 0.0078 0.0005 0.0012 0.0004 0.0009 0.0008 0.0008 0.001 0.0013
Bud Periphery 0.0013 0.0012 0.001 0.0008 0.0009 0.0013 0.0009 0.001 0.001 0.0011 0.0005 0.0006 0.0005 0.0028 0.001 0.0005 0.001 0.0013
Bud Site 0.0025 0.004 0.001 0.0021 0.001 0.0008 0.0031 0.0011 0.0048 0.0128 0.0007 0.0007 0.0005 0.0021 0.0009 0.0007 0.0005 0.0007
Cell Periphery 0.0015 0.0022 0.0016 0.0025 0.0011 0.002 0.0022 0.0023 0.0014 0.0016 0.0008 0.0017 0.0013 0.0024 0.0017 0.0018 0.002 0.002
Cytoplasm 0.0237 0.0322 0.0297 0.0068 0.0056 0.0064 0.0082 0.0185 0.0057 0.0041 0.0055 0.0102 0.0084 0.0264 0.0098 0.0037 0.0022 0.011
Cytoplasmic Foci 0.0197 0.0134 0.0226 0.0102 0.0241 0.0107 0.0166 0.0075 0.0254 0.0283 0.0086 0.006 0.0164 0.0077 0.0099 0.0033 0.0076 0.0073
Eisosomes 0.0004 0 0 0 0 0 0.0001 0 0.0002 0.0013 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0052 0.0039 0.0042 0.0023 0.0009 0.0018 0.027 0.005 0.0033 0.0008 0.001 0.0023 0.0025 0.0056 0.0032 0.0006 0.0012 0.0036
Endosome 0.2537 0.1616 0.2147 0.2489 0.1665 0.1497 0.2464 0.1657 0.1996 0.2565 0.2706 0.1711 0.2192 0.1159 0.1792 0.1365 0.1785 0.1619
Golgi 0.0045 0.0036 0.0056 0.0028 0.0029 0.0026 0.0056 0.0043 0.0086 0.0071 0.0037 0.0036 0.0037 0.0014 0.0033 0.0014 0.0025 0.0022
Lipid Particles 0.0172 0.0093 0.023 0.0195 0.0465 0.0096 0.0144 0.0084 0.0188 0.0105 0.0022 0.0052 0.0194 0.0061 0.0135 0.0015 0.0103 0.0058
Mitochondria 0.0161 0.0155 0.0616 0.0372 0.0014 0.0082 0.0034 0.0108 0.0075 0.0203 0.0022 0.0067 0.0127 0.0038 0.0184 0.0005 0.0063 0.0106
None 0.0991 0.0281 0.0536 0.0048 0.0226 0.01 0.0355 0.0498 0.0016 0.001 0.0002 0.0017 0.0117 0.0135 0.0127 0.0002 0.0012 0.0082
Nuclear Periphery 0.0089 0.0064 0.0098 0.0054 0.0035 0.0046 0.0082 0.0073 0.005 0.0013 0.0019 0.0035 0.0054 0.0085 0.0089 0.0013 0.0018 0.0063
Nucleolus 0.0078 0.0059 0.0062 0.0159 0.0141 0.0052 0.0054 0.0021 0.0074 0.0025 0.0009 0.0019 0.0025 0.0024 0.0053 0.0022 0.007 0.0044
Nucleus 0.0106 0.0096 0.0128 0.0102 0.0045 0.0096 0.0137 0.0117 0.0047 0.0022 0.0019 0.0074 0.0032 0.0059 0.0116 0.0026 0.0034 0.0085
Peroxisomes 0.0013 0.0011 0.002 0.0035 0.0012 0.0011 0.0012 0.0013 0.0074 0.0072 0.0004 0.0007 0.0014 0.0004 0.0011 0.0003 0.0012 0.0007
Punctate Nuclear 0.0042 0.0005 0.0008 0.0006 0.0087 0.0006 0.0011 0.0002 0.0024 0.0003 0.0001 0.0002 0.0006 0.0003 0.0019 0 0.0001 0.0003
Vacuole 0.4254 0.5928 0.4533 0.519 0.5983 0.6397 0.4938 0.579 0.5232 0.5356 0.5655 0.6112 0.518 0.6356 0.5618 0.7412 0.649 0.6134
Vacuole Periphery 0.0869 0.1067 0.095 0.1058 0.0945 0.1344 0.1054 0.1227 0.1424 0.0625 0.1326 0.1637 0.1719 0.1571 0.1545 0.1006 0.123 0.1501

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.0216 32.8792 31.0517 40.3284 45.5195 50.127 38.9107 29.0668 39.3956 47.2855
Translational Efficiency 1.1747 1.6343 1.5471 1.2294 1.168 1.4266 1.3548 1.2339 1.053 1.0698

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
500 1303 1775 934 1653 1176 2230 1830 2153 2479 4005 2764

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 634.51 769.39 789.34 865.13 696.84 759.03 848.63 875.37 682.36 764.48 822.35 871.91
Standard Deviation 76.21 107.34 95.46 113.37 121.67 98.78 98.62 122.35 115.79 103.50 101.59 119.49
Intensity Change Log 2 — 0.278072 0.315004 0.447274 — 0.123329 0.284308 0.329066 — 0.199151 0.299019 0.386612

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000216 0.000142 0.000143 0.001604 0.000026 0.000043 0.000067 0.000738 0.000070 0.000095 0.000100 0.001031
Bud Neck 0.030936 0.020889 0.016514 0.019237 0.015391 0.014276 0.040576 0.021359 0.019001 0.017752 0.029912 0.020642
Bud Site 0.026084 0.018694 0.013503 0.040091 0.006376 0.010643 0.015934 0.034136 0.010953 0.014875 0.014857 0.036148
Cell Periphery 0.001553 0.000135 0.000060 0.000145 0.000179 0.000372 0.000049 0.000077 0.000498 0.000248 0.000054 0.000100
Cytoplasm 0.161669* 0.134508* 0.466938* 0.226859* 0.127628* 0.029832 0.286886* 0.148923* 0.135534* 0.084851 0.366684* 0.175259*
Cytoplasmic Foci 0.136898 0.079495 0.024199 0.078805 0.058878 0.029953 0.018827 0.068464 0.076997 0.055993 0.021208 0.071959
Eisosomes 0.000227 0.000038 0.000021 0.000033 0.000043 0.000025 0.000021 0.000021 0.000086 0.000032 0.000021 0.000025
Endoplasmic Reticulum 0.006459 0.001311 0.002248 0.001886 0.002067 0.003061 0.001945 0.001077 0.003087 0.002141 0.002080 0.001350
Endosome 0.148585 0.090101 0.014030 0.107658 0.076148 0.077691 0.066861 0.173752 0.092971 0.084214 0.043446 0.151418
Golgi 0.006060 0.003783 0.000330 0.005292 0.002207 0.003758 0.000571 0.011253 0.003102 0.003771 0.000464 0.009239
Lipid Particles 0.014405 0.002426 0.001341 0.002519 0.003925 0.002012 0.001583 0.001725 0.006359 0.002230 0.001476 0.001993
Mitochondria 0.011168 0.005346 0.001350 0.002208 0.001515 0.008936 0.001830 0.004076 0.003757 0.007049 0.001617 0.003445
Mitotic Spindle 0.000267 0.003792 0.018032 0.027912 0.000297 0.002330 0.016050 0.015457 0.000290 0.003098 0.016929 0.019666
None 0.000827 0.000703 0.004613 0.000971 0.004821 0.000226 0.002276 0.001042 0.003894 0.000477 0.003312 0.001018
Nuclear Periphery 0.000423 0.000251 0.003472 0.001326 0.000383 0.000235 0.003571 0.000705 0.000392 0.000244 0.003527 0.000915
Nuclear Periphery Foci 0.000204 0.000352 0.002098 0.002263 0.000369 0.000103 0.001022 0.000570 0.000331 0.000234 0.001499 0.001142
Nucleolus 0.000756 0.001592 0.003005 0.002213 0.003344 0.000505 0.008932 0.001685 0.002743 0.001077 0.006305 0.001864
Nucleus 0.016248 0.014817 0.317478* 0.052258 0.037161 0.007664 0.162935* 0.018498 0.032304 0.011424 0.231428* 0.029906
Peroxisomes 0.004093 0.005045 0.001509 0.011106 0.001482 0.001660 0.001559 0.007696 0.002089 0.003439 0.001537 0.008849
Vacuole 0.402164* 0.589669* 0.108276 0.408198* 0.636179* 0.764776* 0.363372* 0.464066* 0.581833* 0.672737* 0.250314* 0.445187*
Vacuole Periphery 0.030760 0.026911 0.000839 0.007414 0.021579 0.041898 0.005131 0.024679 0.023711 0.034021 0.003229 0.018845

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.89 0.34 -1.36 -1.51 -1.42 -1.50 -5.79 -1.56 -1.52 -1.40 -1.06 -3.23 -2.12 -2.04 -1.92
Bud Neck 3.34 3.62 4.05 1.11 0.62 0.62 -11.58 -3.74 -3.85 8.75 0.86 -7.64 -1.02 -1.94 7.11
Bud Site 1.58 3.12 0.01 -2.35 -4.66 -3.01 -5.07 -11.72 -8.31 -7.49 -2.56 -0.87 -9.09 -7.45 -8.93
Cell Periphery 3.30 3.47 3.38 1.15 -1.45 -1.72 5.33 4.22 2.76 -3.57 2.19 4.36 4.07 3.13 -2.76
Cytoplasm 2.36 -23.13 -7.51 -11.41 15.29 18.07 -22.77 -6.44 -23.24 15.07 9.23 -35.09 -10.38 -19.73 22.75
Cytoplasmic Foci 6.82 14.81 10.37 5.55 -8.13 8.32 15.25 -1.29 -10.11 -18.58 5.87 19.86 5.14 -1.31 -19.27
Eisosomes 9.21 9.97 9.33 -0.18 -6.05 4.79 6.46 6.47 1.28 0.41 9.00 11.13 10.43 1.66 -3.22
Endoplasmic Reticulum 6.39 4.39 5.23 -5.38 3.81 -3.12 0.00 4.50 7.53 8.91 3.38 2.53 6.32 4.48 9.91
Endosome 6.81 17.12 9.98 5.34 -10.30 -0.31 7.53 -12.54 -11.14 -18.17 2.30 18.59 -5.12 -7.32 -21.69
Golgi 1.72 4.85 2.69 1.53 -3.95 -1.79 3.55 -5.77 -4.10 -7.95 -1.02 5.78 -4.28 -3.54 -8.69
Lipid Particles 7.43 8.00 7.51 0.14 -4.18 4.33 6.44 5.55 0.42 -1.66 8.25 10.06 9.06 0.87 -3.59
Mitochondria 2.95 5.46 5.29 4.44 -0.25 -4.69 -0.63 -3.96 3.26 -3.47 -3.41 4.79 1.64 4.56 -3.45
Mitotic Spindle -3.33 -9.55 -6.86 -5.52 -0.11 -2.26 -8.71 -8.20 -6.49 0.50 -4.00 -12.87 -10.68 -8.36 0.57
None 0.16 -8.34 -2.08 -3.66 9.09 3.83 1.99 2.94 -1.39 2.56 3.65 0.67 2.69 -1.94 5.32
Nuclear Periphery 1.78 -13.13 -11.35 -14.24 6.64 3.19 -14.04 -6.66 -8.22 11.73 3.78 -19.39 -12.89 -15.31 13.96
Nuclear Periphery Foci -1.68 -9.04 -10.20 -9.04 1.31 6.27 -5.69 -3.56 -7.11 3.74 1.56 -10.41 -9.94 -10.22 4.23
Nucleolus -1.92 -8.10 -4.69 -0.56 3.95 7.14 -7.87 3.77 -6.89 11.07 3.08 -8.75 2.23 -1.75 11.78
Nucleus 0.44 -46.36 -14.66 -15.95 27.69 10.70 -30.31 3.34 -10.56 35.28 9.42 -50.32 -6.76 -17.69 45.17
Peroxisomes -1.21 5.42 -1.60 -0.58 -4.04 -0.46 0.80 -6.72 -6.08 -7.00 -2.97 3.56 -6.77 -3.86 -8.00
Vacuole -10.89 17.57 -1.98 11.48 -28.27 -11.57 29.31 15.52 27.75 -13.90 -9.41 39.10* 13.24 25.10 -29.39
Vacuole Periphery 1.41 11.72 10.31 14.96 -5.89 -8.75 15.08 0.79 9.28 -13.80 -6.26 19.63 6.65 13.03 -14.93
ORF
Human Ortholog
Description Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress
Localization
Cell Percentages vacuole (74%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YNR061C

YNR061C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynr061c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available