ORF
Description Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.3 0.43 0.58 0.37 0.74 0.77 0.83 – 0.8 0.8 0.85 0.42 0.8 0.96 0.25 0.32 0.23 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0.12 0 0.15 0.14 0.15 0.18 – 0.21 0.21 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.13 0.08 0 0.12 0.12 0.08 0 0 – 0 0 0 0.07 0 0 0.3 0.17 0.28 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.8 0.58 0.66 0.69 0.32 0.2 0.16 – 0.2 0.15 0.13 0.58 0.26 0.06 0.6 0.64 0.55 0.87 0.91 0.87 0.84 0.81 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 3 5 3 – 2 8 5 0 0 0 0 0 0 1 1 3 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 1 0 0 0 1 2 1 0 0 0 0 0 0 0 0
Cytoplasm 43 116 143 149 111 337 355 393 – 395 377 438 103 205 325 46 41 28 4 8 16 5 6 2
Endoplasmic Reticulum 0 2 1 0 0 0 1 0 – 3 0 0 3 5 2 9 1 2 0 0 1 0 3 2
Endosome 6 6 21 1 3 0 0 3 – 1 0 1 12 1 0 5 1 5 8 12 9 4 7 6
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 1 3 1 0 0 1 2
Mitochondria 20 13 40 11 46 62 69 84 – 103 100 112 2 0 1 4 3 4 5 5 7 2 6 5
Nucleus 35 31 6 30 35 35 20 9 – 3 6 7 17 7 5 55 22 35 6 4 1 2 1 1
Nuclear Periphery 2 0 0 5 5 0 20 2 – 5 2 2 0 0 0 0 0 0 3 0 0 1 3 2
Nucleolus 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 5 0 0 1 1 0 – 0 0 1 0 0 0 0 0 1 1 0 0 2 1 0
Vac/Vac Membrane 209 314 190 170 206 144 92 75 – 99 72 68 141 67 22 108 83 68 261 368 302 150 159 157
Unique Cell Count 268 393 330 259 300 457 461 475 496 470 517 243 257 340 181 129 123 299 407 349 178 197 189
Labelled Cell Count 315 482 407 366 407 583 563 569 612 565 634 279 286 357 230 155 144 299 407 349 178 197 189


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.3 4.6 4.6 4.4 4.0 3.6 3.7 – 3.5 3.6 3.6 5.6 4.9 4.9 6.5 6.6 6.5 6.7 6.6 6.7
Std Deviation (1e-4) 1.1 1.0 1.4 1.3 1.3 1.0 1.1 1.2 – 1.0 1.1 1.0 1.0 0.8 0.8 1.4 1.5 1.7 1.4 1.8 1.5
Intensity Change (Log2) – – – 0.01 -0.05 -0.2 -0.35 -0.28 – -0.4 -0.35 -0.35 0.3 0.12 0.12 0.51 0.52 0.52 0.55 0.53 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 3.4 -1.6 8.6 9.7 11.7 – 10.7 10.8 12.7 -0.2 8.9 14.7 -4.0 -2.3 -4.0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 -0.7 -3.8 -4.7 -1.8 -2.5 -0.9
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -5.1 -5.8 -6.4 -3.8 -3.2 -2.8
Nucleus 4.9 5.0 3.6 2.0 0.1 – 0 0 -0.5 3.1 0.7 -0.4 9.5 6.1 8.8
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 2.0 2.9 -7.3 -10.9 -12.4 – -11.1 -12.5 -13.7 0.1 -7.6 -14.2 0.5 1.3 -0.4