Standard name
Description Nucleosome assembly factor; involved in chromatin assembly, disassembly; required for recovery after DSB repair; role in H3K56 acetylation required for expression homeostasis, buffering mRNA synthesis rate against gene dosage changes in S phase; anti-silencing protein, derepresses silent loci when overexpressed; role in regulating Ty1 transposition; relocalizes to cytosol under hypoxia; growth defect of asf1 null is functionally complemented by either human ASF1A or ASF1B
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.12 0.11 0.07 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.89 0.96 0.98 0.94 0.9 0.89 0.83 0.81 0.8 0.82 0.83 0.93 0.85 0.83 0.86 0.91 0.89 0.95 0.91 0.96 0.83 0.75 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.1 0.13 0.19 0.28 0.27 0.32 0.3 0.06 0 0.05 0 0.05 0 0 0 0 0 0 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.13 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3
Bud 0 0 0 0 0 0 1 1 1 0 1 2 0 0 0 0 0 1 1 2 1 3 1 3
Bud Neck 0 0 0 0 0 1 3 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4 0 1 16 8 9 20 0 3 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 13 9 7 12 15 13 22 7 13 4 7 3 29 30 17 13 16 0 4 1 6 3 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 6 0 3 0 0 0 3 0 0
Endosome 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 2 0 1 0 2 2 5
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 3 6
Mitochondria 1 18 0 1 1 5 14 5 43 26 33 28 1 2 0 8 10 2 0 0 0 3 11 9
Nucleus 93 221 370 334 420 522 682 617 518 552 538 590 108 205 232 211 241 289 104 231 377 174 236 151
Nuclear Periphery 0 2 2 2 3 1 7 4 3 9 2 3 0 2 2 3 2 1 0 0 0 0 0 1
Nucleolus 1 4 3 3 13 57 101 138 178 188 214 213 7 9 15 7 13 14 1 1 1 1 8 23
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4 2 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 2 1 0 2 1 0 3 7 1 0 0 0 1 0 0 2 1
Vac/Vac Membrane 1 8 6 1 4 9 7 11 8 12 2 5 2 1 5 4 5 15 1 6 5 8 41 42
Unique Cell Count 96 249 386 342 445 580 764 744 639 688 659 712 116 241 281 244 264 326 110 254 391 211 315 260
Labelled Cell Count 96 267 390 349 455 614 828 804 778 809 806 869 121 256 297 259 284 343 110 254 391 211 315 260


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.9 8.6 12.6 11.9 13.6 12.0 11.6 12.8 10.7 11.5 10.5 10.9 10.7 13.5 13.2 15.1 19.3 17.8 11.3 11.3 11.6
Std Deviation (1e-4) 3.1 3.0 3.8 4.1 4.8 4.5 4.1 4.5 3.7 4.3 3.3 4.1 4.8 6.6 6.4 6.8 7.9 8.9 3.1 2.5 3.2
Intensity Change (Log2) – – – -0.08 0.11 -0.07 -0.12 0.02 -0.24 -0.13 -0.26 -0.2 -0.24 0.1 0.06 0.26 0.62 0.5 -0.15 -0.15 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.1 0 0 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 0.3 0.2 -0.7 0.6 -1.5 -0.5 0 -1.8 0 5.0 4.5 2.8 1.8 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.2 3.9 4.5 3.9 0 0 0 0 0 0
Nucleus 1.4 -1.0 -3.4 -3.8 -6.2 -6.7 -7.0 -6.6 -6.2 -1.2 -4.8 -5.7 -4.3 -2.4 -3.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.2 5.7 6.9 8.6 11.0 10.9 12.2 11.6 0 0 3.6 0 3.4 3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 -0.1 -0.4 0.2 0 0 0 0 0 0 0 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.5647 15.2204 15.5649 14.1717 13.5876 13.3956 11.2703 15.5324 14.4032 10.7514 12.4538 12.243 7.9698 11.3257 10.9614 10.1588 7.7353 8.9174
Actin 0.0209 0 0 0 0.0262 0 0.0164 0 0.0139 0.0007 0 0.0052 0.0027 0 0.0019 0 0.0358 0
Bud 0.0001 0 0 0 0.0001 0 0.0004 0 0.0005 0.0021 0 0 0.0002 0 0.0001 0 0.0002 0
Bud Neck 0.0003 0 0 0 0.0004 0.0004 0.0005 0 0.0002 0.0004 0 0.0006 0.0002 0 0.0006 0 0.0003 0.0003
Bud Periphery 0.0002 0 0 0 0.0002 0.0001 0.0012 0 0.0011 0.0061 0 0.0001 0.0004 0 0 0 0.0004 0.0001
Bud Site 0.0005 0 0 0 0.0002 0.0001 0.0005 0 0.0009 0.0025 0 0 0.0007 0 0.0037 0 0.0002 0
Cell Periphery 0.0001 0 0 0 0.0001 0.0001 0.0005 0 0.0003 0.0021 0 0.0001 0.0005 0 0 0 0.0001 0.0001
Cytoplasm 0.0005 0 0 0 0 0 0.0051 0 0.0008 0.0003 0 0.0003 0.0008 0 0.0001 0 0.0002 0.0002
Cytoplasmic Foci 0.0013 0 0 0 0.0002 0 0.0266 0 0.0033 0.0011 0 0.006 0.0155 0 0.0005 0 0.0029 0.0001
Eisosomes 0.0002 0 0 0 0.0002 0 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0 0 0.0004 0
Endoplasmic Reticulum 0.0003 0 0 0 0.0001 0 0.0012 0 0.0008 0.0004 0 0.0001 0.0003 0 0 0 0.0009 0
Endosome 0.0013 0 0 0 0.0005 0 0.0119 0 0.0038 0.0021 0 0.0008 0.0075 0 0.0001 0 0.0086 0
Golgi 0.0018 0 0 0 0.0016 0 0.0034 0 0.0017 0.0004 0 0.0008 0.0032 0 0.0002 0 0.0132 0
Lipid Particles 0.0032 0 0 0 0.0007 0.0001 0.0129 0 0.0032 0.0017 0 0.0016 0.016 0 0.0002 0 0.0341 0
Mitochondria 0.0036 0.0003 0.0001 0.0001 0.0008 0.0002 0.004 0.0001 0.0094 0.057 0.0006 0.0003 0.0088 0.0002 0.0001 0.0002 0.0203 0.0002
None 0.0007 0 0 0 0.0001 0 0.004 0 0.0009 0.0004 0 0.001 0.0014 0 0.0001 0 0.0006 0.0001
Nuclear Periphery 0.0014 0.0004 0.0005 0.0003 0.0003 0.0004 0.026 0.0006 0.0059 0.0019 0.0002 0.0002 0.0027 0.0006 0.0002 0.0003 0.0046 0.0004
Nucleolus 0.0299 0.0058 0.0054 0.01 0.0855 0.0606 0.025 0.006 0.0169 0.0511 0.0443 0.0376 0.0178 0.0123 0.005 0.0087 0.0843 0.0354
Nucleus 0.9214 0.9934 0.994 0.9895 0.8821 0.9371 0.8165 0.9931 0.919 0.8514 0.9545 0.9404 0.8957 0.9865 0.9865 0.9906 0.7815 0.9623
Peroxisomes 0.0072 0 0 0 0.0004 0 0.0156 0 0.0031 0.0017 0 0.0034 0.0082 0 0.0002 0 0.0031 0
Punctate Nuclear 0.0044 0 0 0.0001 0.0001 0.0004 0.0228 0 0.0086 0.0003 0 0.0009 0.013 0.0004 0.0004 0 0.0023 0.0003
Vacuole 0.0003 0 0 0 0.0002 0.0003 0.0025 0 0.0025 0.0091 0.0001 0.0003 0.0026 0 0 0 0.0008 0.0003
Vacuole Periphery 0.0003 0 0 0 0.0001 0.0001 0.0029 0 0.0032 0.0071 0.0002 0.0001 0.0016 0 0 0.0001 0.0054 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.153 12.9168 11.3275 20.9474 37.4684 15.7542 8.9047 10.8181 23.0061 30.5185
Translational Efficiency 4.7919 2.3381 2.0154 3.7719 3.095 10.1271 3.1878 1.6078 1.7046 3.044

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘