Standard name
Description Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.09 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0 0.1 0.08 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.91 0.92 0.97 0.88 0.81 0.85 0.92 0.96 0.94 0.94 0.91 0.96 0.97 0.99 0.87 0.85 0.86 0.23 0.34 0.3 0.39 0.4 0.56
Endoplasmic Reticulum 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.05 0.2 0.15 0.09 0.06 0.1 0.12 0.17 0 0 0 0 0 0 0.06 0 0.17 0.06 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0.13 0.17 0.14 0.41 0.37 0.28 0.23 0.22 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 2 0 1 2 0 2 1 0 1 0 0 7 2 0 1 0 0 2 13 4 8 20 11
Bud 1 4 3 0 5 3 4 6 5 3 4 4 3 1 0 0 1 0 3 15 1 11 10 7
Bud Neck 0 4 4 1 0 0 1 6 1 1 1 2 3 1 0 3 3 0 9 28 5 14 22 12
Bud Site 1 1 1 0 1 0 0 1 0 1 0 0 2 0 0 0 0 0
Cell Periphery 2 4 1 3 1 0 0 2 0 0 1 0 1 1 2 3 1 4 0 3 5 2 5 6
Cytoplasm 62 272 141 118 143 113 155 195 137 182 147 163 325 222 240 413 321 272 21 112 41 54 110 164
Endoplasmic Reticulum 7 6 4 0 4 2 3 0 1 0 0 0 2 0 1 33 14 20 2 3 1 1 10 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 6 2 5 5 8 4 1 5 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 2 1 1 0 2 1
Mitochondria 1 6 6 6 8 28 28 20 8 20 18 30 1 0 1 10 1 7 5 12 24 8 18 13
Nucleus 0 0 0 1 3 2 5 3 0 1 1 0 0 1 0 11 4 5 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 2 2 0 0 1 0 0 0 0 1 0 3 0 0 3 5 6 0 2 2 0 3 1
Vac/Vac Membrane 0 14 0 1 8 8 8 5 2 3 2 5 1 0 0 64 63 44 39 122 39 31 61 61
Unique Cell Count 73 299 153 122 162 140 182 211 142 193 156 179 338 228 242 475 377 317 95 333 140 138 278 294
Labelled Cell Count 78 316 163 131 175 157 206 240 154 213 175 204 350 232 244 550 419 364 95 333 140 138 278 294


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.1 3.1 3.1 3.5 3.3 3.2 3.4 3.3 2.8 3.0 2.6 4.3 4.2 3.8 6.3 6.7 6.6 4.4 4.4 5.0
Std Deviation (1e-4) 1.1 0.8 0.6 0.9 1.2 0.8 1.0 1.1 1.8 1.3 1.2 0.8 0.9 0.9 0.9 1.7 2.2 2.5 1.0 1.3 1.4
Intensity Change (Log2) -0.02 0.19 0.1 0.04 0.11 0.07 -0.12 -0.06 -0.23 0.46 0.44 0.28 1.01 1.11 1.09 0.49 0.51 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 4.8 5.4 4.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dse4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available