Standard name
Description GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.09 0.09 0.1 0.15 0.08 0.09 0.08 0.09 0.13 0.18 0.25 0.21 0.16 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0.08 0.21 0.31 0.71 0.66 0.64 0.77 0.79 0.8 0.82 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0.92 0.95 0.9 0.87 0.63 0.45 0.47 0.42 0.43 0.38 0.37 0.37 0.82 0.75 0.67 0.69 0.76 0.74 0.85 0.85 0.79 0.71 0.61 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Vac/Vac Membrane 0 0 0 0 0.07 0.08 0.05 0 0 0 0.05 0.05 0 0.07 0.07 0.05 0.06 0.08 0.06 0.06 0.1 0.11 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1
Bud 0 0 1 0 4 1 10 6 7 17 7 9 0 0 0 0 0 1 3 4 4 1 4 8
Bud Neck 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 1 0 0 0 2 6
Bud Site 3 0 0 2 1 8 14 9 13 30 5 15 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Cytoplasm 10 5 15 9 33 37 58 99 49 62 25 38 52 75 117 88 43 30 0 0 3 0 1 1
Endoplasmic Reticulum 0 0 2 0 1 1 0 1 2 1 0 0 0 0 0 14 10 4 0 0 1 0 1 2
Endosome 1 3 3 0 16 4 4 5 0 1 0 0 2 2 11 16 6 5 3 3 7 2 12 13
Golgi 0 1 2 0 1 0 0 0 0 0 0 0 1 0 0 1 1 0 3 2 0 1 2 2
Mitochondria 105 8 36 53 108 296 368 429 444 556 240 359 6 1 4 28 2 11 2 4 3 3 5 7
Nucleus 503 414 412 220 221 187 262 285 249 266 111 161 321 317 315 282 208 146 471 390 387 141 199 226
Nuclear Periphery 16 0 6 3 0 1 1 4 5 0 1 3 0 1 0 0 0 1 2 0 1 0 2 3
Nucleolus 41 6 10 10 5 2 8 9 7 15 5 16 6 1 0 2 0 1 16 17 18 19 36 30
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 2 3 0 0 3 8 3 8 1 3 4 2 0 9 4 6 6 4 7 3 16 20
Vac/Vac Membrane 5 10 8 2 25 32 29 25 21 24 14 21 9 29 32 22 17 15 35 29 46 22 32 71
Unique Cell Count 549 435 459 252 349 419 556 673 579 700 301 439 392 420 471 411 274 197 557 461 490 200 326 404
Labelled Cell Count 686 448 497 302 415 569 757 881 800 980 412 625 402 430 479 462 291 220 557 461 490 200 326 404


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.3 5.5 4.7 4.4 3.6 3.3 3.5 3.4 2.9 3.4 3.1 6.1 5.2 5.1 6.8 7.6 7.2 5.2 5.4 5.8
Std Deviation (1e-4) 0.8 1.2 1.3 1.4 1.9 1.1 1.4 1.2 0.8 0.8 1.6 0.9 1.3 1.2 1.4 1.7 1.8 1.6 1.1 1.5 1.3
Intensity Change (Log2) – – – -0.21 -0.31 -0.6 -0.71 -0.63 -0.71 -0.9 -0.71 -0.8 0.14 -0.07 -0.1 0.31 0.47 0.39 -0.07 -0.04 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 3.7 3.5 4.4 6.3 3.5 3.7 3.0 3.4 5.4 7.1 9.4 8.3 6.0 5.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 2.1 3.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.2 -5.6 -5.3 -0.6 -4.2 -1.0
Nucleus -1.0 -9.0 -14.3 -14.3 -16.1 -15.6 -17.5 -15.4 -16.5 -3.3 -5.6 -8.4 -7.8 -5.0 -5.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 -0.7 -2.6 0 -2.1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0 0
Vacuole 0 3.9 4.2 2.9 1.9 1.8 1.7 2.3 2.6 0.6 3.8 3.8 2.9 3.2 3.7