Standard name
Description 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.98 0.93 0.84 0.77 0.81 0.69 0.71 0.65 0.7 0.97 0.96 0.97 0.93 0.91 0.93 0.89 0.9 0.93 0.89 0.88 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.12 0.05 0.29 0.17 0.28 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.1 0.18 0.13 0.12 0.2 0.13 0.14 0 0 0 0.08 0.13 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 1 1 1 1 3
Bud 0 2 1 0 0 1 1 1 6 3 19 10 0 1 0 0 0 0 12 5 5 4 12 19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 1 4 8 7 3 4 4 5 5 0 2 5 12 11 5 0 0 0 0 0 0
Cytoplasm 281 203 289 298 452 317 361 347 192 235 225 248 323 548 600 289 324 260 259 181 270 251 422 413
Endoplasmic Reticulum 0 1 0 0 1 4 6 7 3 5 1 0 3 3 3 4 6 4 1 1 1 0 5 3
Endosome 0 1 0 0 0 0 0 0 0 1 0 0 1 2 2 0 1 1 3 0 1 1 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 2 2
Mitochondria 4 2 0 1 6 34 54 22 80 57 96 57 0 0 3 2 0 1 0 0 2 2 4 0
Nucleus 2 1 0 2 4 4 5 14 2 9 10 9 2 9 3 1 1 2 1 3 2 0 2 1
Nuclear Periphery 0 0 0 0 1 1 0 0 0 4 2 0 0 0 0 0 0 0 0 0 1 0 2 1
Nucleolus 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 3 5 8
Vac/Vac Membrane 0 2 6 7 35 37 85 54 34 68 45 48 11 22 17 25 46 17 6 4 1 8 9 7
Unique Cell Count 285 208 295 305 485 376 466 430 278 333 344 353 334 568 621 312 355 279 293 203 292 282 478 475
Labelled Cell Count 287 212 299 309 504 406 520 450 322 386 403 380 340 587 633 334 390 290 293 203 292 282 478 475


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 10.4 8.1 8.1 8.3 7.1 7.2 7.9 6.9 7.8 7.2 8.2 12.9 13.0 13.2 19.1 21.1 20.2 7.8 8.1 8.3
Std Deviation (1e-4) 2.5 3.7 2.1 2.5 2.3 1.9 2.0 2.3 2.5 2.9 3.7 3.6 5.8 5.4 6.0 7.6 11.1 8.0 2.2 2.5 2.3
Intensity Change (Log2) 0.01 0.03 -0.18 -0.17 -0.04 -0.24 -0.06 -0.17 0.01 0.67 0.68 0.7 1.23 1.38 1.32 -0.06 -0.01 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.8 2.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.6 2.2 0
Cytoplasm -0.2 -3.0 -5.9 -7.8 -7.0 -9.4 -9.2 -10.3 -9.3 -1.0 -1.2 -1.1 -3.1 -3.7 -2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 6.1 3.9 9.9 7.5 9.8 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 3.1 4.1 6.7 5.1 4.8 7.1 5.1 5.3 1.0 1.4 0.6 3.3 5.1 2.5