Standard name
Description 6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.91 0.85 0.87 0.83 0.75 0.53 0.55 0.42 0.46 0.34 0.43 0.87 0.91 0.88 0.5 0.67 0.67 0.59 0.78 0.82 0.76
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0.09 0 0.08 0 0 0 0 0 0 0 0 0 0.07 0.12 0.08 0.07 0.15 0.09 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0.09 0.3 0.53 0.42 0.63 0.65 0.77 0.65 0 0 0 0 0.1 0.11 0.1 0 0 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0.07 0.05 0.06 0.13 0.19 0.13 0.14 0.13 0.18 0 0 0 0.38 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 1 0 0 1 0 0 1 0 0 3 3 2 0 9 1 6 0 3 6
Bud 6 1 4 1 2 6 21 10 24 20 18 18 0 0 1 0 2 0 1 0 2 5
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 2 0 1 4 2 1 2 3
Bud Site 0 0 0 0 0 0 0 2 1 3 1 5 0 0 0 0
Cell Periphery 1 0 0 0 2 3 1 1 0 3 2 1 1 3 3 0 0 0 0 0 2 0
Cytoplasm 251 61 171 267 360 345 397 374 234 295 152 237 181 284 343 8 222 73 144 81 236 265
Endoplasmic Reticulum 11 4 5 7 2 3 5 2 0 3 2 1 20 11 11 1 8 1 5 0 1 3
Endosome 3 0 17 10 36 17 21 29 3 2 1 1 5 12 28 2 26 7 36 9 10 17
Golgi 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 2 0 0 0
Mitochondria 26 3 4 13 41 136 393 282 351 418 346 358 0 0 2 0 33 11 24 2 9 24
Nucleus 0 1 0 0 0 2 11 12 13 14 2 13 1 1 0 0 0 0 0 2 1 1
Nuclear Periphery 5 0 0 0 0 2 10 2 5 0 0 5 0 0 0 0 1 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 1 0 1 1 2 0 2 4 5 1 0 0 2 1 4 1 12 2 7 1 7 8
Vac/Vac Membrane 7 0 12 23 22 29 98 125 71 91 59 98 1 8 16 6 8 4 3 1 3 6
Unique Cell Count 292 67 200 307 432 460 746 675 555 647 452 547 208 312 392 16 332 109 245 105 288 351
Labelled Cell Count 312 70 216 324 467 543 960 845 709 851 583 739 214 323 414 18 332 109 245 105 288 351


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.7 5.3 6.3 5.4 4.6 4.3 4.4 4.0 3.9 3.7 3.7 7.3 7.1 6.4 8.1 7.0 7.1 6.9
Std Deviation (1e-4) 0.6 0.7 1.3 1.7 1.6 1.1 1.1 1.2 1.1 1.1 1.2 1.0 1.3 1.2 1.2 2.0 1.5 1.5 1.4
Intensity Change (Log2) 0.24 0.03 -0.21 -0.31 -0.28 -0.42 -0.44 -0.52 -0.52 0.46 0.42 0.26 0.61 0.39 0.41 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pfk27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available