Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 1 | 2 | 4 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 2 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 1 | 0 | 0 | 0 | 4 | 7 | 3 | – | 20 | 13 | 20 | 21 | 0 | 2 | 1 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 8 | 11 | 14 | 17 | 16 | 18 | – | 11 | 16 | 12 | 9 | 28 | 76 | 94 | 18 | 21 | 24 | 0 | 2 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 2 | 0 |
Endosome | 2 | 2 | 5 | 0 | 5 | 2 | 4 | – | 1 | 6 | 5 | 1 | 1 | 4 | 7 | 1 | 0 | 0 | 1 | 5 | 4 | 4 | 3 | 9 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 3 | 2 | 2 | 2 | 3 | 4 |
Mitochondria | 1 | 21 | 1 | 5 | 6 | 40 | 10 | – | 103 | 38 | 91 | 79 | 0 | 1 | 1 | 7 | 3 | 9 | 0 | 3 | 1 | 1 | 3 | 7 |
Nucleus | 312 | 321 | 376 | 387 | 355 | 375 | 556 | – | 516 | 510 | 428 | 599 | 291 | 387 | 494 | 141 | 168 | 164 | 282 | 345 | 353 | 256 | 346 | 356 |
Nuclear Periphery | 1 | 0 | 4 | 3 | 1 | 5 | 6 | – | 8 | 5 | 3 | 7 | 1 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 95 | 48 | 55 | 27 | 43 | 64 | 116 | – | 217 | 220 | 152 | 258 | 65 | 81 | 75 | 22 | 14 | 22 | 64 | 30 | 82 | 120 | 167 | 142 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 3 | 1 | 5 | 4 | 21 | – | 2 | 15 | 8 | 9 | 1 | 6 | 15 | 4 | 2 | 2 | 1 | 2 | 3 | 4 | 6 | 12 |
Vac/Vac Membrane | 2 | 9 | 2 | 2 | 9 | 11 | 10 | – | 3 | 5 | 4 | 6 | 2 | 6 | 5 | 4 | 4 | 2 | 11 | 13 | 7 | 14 | 31 | 36 |
Unique Cell Count | 352 | 370 | 421 | 414 | 407 | 455 | 675 | 693 | 689 | 578 | 813 | 346 | 515 | 643 | 181 | 200 | 207 | 373 | 410 | 463 | 409 | 581 | 589 | |
Labelled Cell Count | 420 | 411 | 457 | 440 | 446 | 524 | 747 | 883 | 833 | 728 | 1001 | 390 | 571 | 702 | 201 | 216 | 227 | 373 | 410 | 463 | 409 | 581 | 589 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.2 | 7.6 | 8.7 | 8.4 | 8.3 | 6.4 | 7.1 | – | 6.2 | 6.6 | 6.3 | 6.3 | 9.1 | 8.7 | 9.0 | 9.2 | 10.4 | 8.6 | 8.0 | 8.4 | 8.7 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.7 | 1.8 | 1.7 | 1.8 | 1.6 | – | 1.3 | 1.3 | 1.4 | 1.3 | 2.8 | 2.5 | 2.6 | 2.3 | 2.1 | 2.0 | 2.0 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.08 | -0.44 | -0.29 | – | -0.49 | -0.41 | -0.47 | -0.47 | 0.07 | -0.0 | 0.04 | 0.07 | 0.25 | -0.01 | -0.12 | -0.06 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 3.5 | 0 | 3.9 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 1.2 | 0.8 | 0.1 | – | -1.2 | -0.3 | -0.6 | -2.0 | 3.4 | 6.4 | 6.4 | 3.9 | 4.2 | 4.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.0 | 0 | – | 8.1 | 4.6 | 8.4 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.1 | -0.9 | -2.9 | -3.1 | – | -6.0 | -6.2 | -6.0 | -6.4 | -2.1 | -5.6 | -5.2 | -3.7 | -1.9 | -3.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.2 | -1.1 | 0.4 | 1.8 | – | 6.9 | 7.1 | 5.1 | 7.1 | 2.2 | 1.2 | -0.7 | -0.3 | -2.2 | -0.9 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2.6 | – | 0 | 1.9 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 |
Vacuole | 0 | 2.2 | 2.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9677 | 3.2554 | 2.9744 | 2.7698 | 2.752 | 2.7359 | 5.8667 | 5.3278 | 4.5961 | 4.6872 | 4.4467 | 5.0203 | 7.7486 | 7.5784 | 7.3528 | 7.341 | 7.4434 | 7.3303 |
Actin | 0.0219 | 0.0002 | 0.0035 | 0.0004 | 0.0032 | 0.0012 | 0.0394 | 0 | 0.0073 | 0.0137 | 0.0363 | 0.005 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0003 | 0.0002 | 0.001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.001 | 0.0034 | 0.0001 | 0.0003 | 0.0005 | 0.0016 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0006 |
Bud Periphery | 0.0004 | 0 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0019 | 0 | 0.0007 | 0.0002 | 0.0021 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0.0005 | 0.0001 | 0.0008 | 0.0004 | 0.0006 | 0.0001 | 0.0038 | 0 | 0.001 | 0.0012 | 0.0031 | 0.0002 | 0 | 0 | 0 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0011 | 0.0001 | 0.0042 | 0.0002 | 0.0002 | 0.0002 | 0.0175 | 0 | 0.0029 | 0.0008 | 0.0021 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0021 | 0.0005 | 0.0019 | 0.0007 | 0.0093 | 0.0001 | 0.0075 | 0 | 0.0037 | 0.0102 | 0.0104 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.0002 | 0.0004 | 0.0006 | 0 | 0.0004 | 0.0075 | 0 | 0.0029 | 0.0002 | 0.0022 | 0.001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0098 | 0.0006 | 0.0011 | 0.0024 | 0.0031 | 0.0017 | 0.0216 | 0 | 0.0102 | 0.0027 | 0.0186 | 0.0043 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0033 | 0.0002 | 0.0005 | 0.0006 | 0.0066 | 0.0002 | 0.0067 | 0 | 0.0023 | 0.0012 | 0.0153 | 0.0018 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0055 | 0.0038 | 0.0007 | 0.0051 | 0.0034 | 0.0001 | 0.0078 | 0 | 0.0082 | 0.0034 | 0.0487 | 0.0021 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0031 | 0.0008 | 0.0068 | 0.005 | 0.0014 | 0.0005 | 0.0055 | 0.0001 | 0.0125 | 0.0009 | 0.0137 | 0.003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
None | 0.0024 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0001 | 0.0197 | 0 | 0.0019 | 0.0019 | 0.0015 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0091 | 0.0052 | 0.0059 | 0.0073 | 0.0039 | 0.0018 | 0.044 | 0.0003 | 0.0121 | 0.0041 | 0.0153 | 0.0109 | 0.0007 | 0.0003 | 0.0009 | 0.0004 | 0.0022 | 0.0001 |
Nucleolus | 0.03 | 0.0266 | 0.0206 | 0.0267 | 0.1527 | 0.0908 | 0.0227 | 0.0189 | 0.0198 | 0.0247 | 0.1174 | 0.0564 | 0.0263 | 0.0467 | 0.0264 | 0.0183 | 0.1311 | 0.0737 |
Nucleus | 0.8926 | 0.9515 | 0.9473 | 0.9426 | 0.781 | 0.8995 | 0.7605 | 0.9801 | 0.9018 | 0.9111 | 0.6594 | 0.894 | 0.9717 | 0.9516 | 0.9715 | 0.9808 | 0.8552 | 0.9212 |
Peroxisomes | 0.0069 | 0.0002 | 0.0003 | 0.0003 | 0.013 | 0 | 0.003 | 0 | 0.0021 | 0.0091 | 0.0173 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0053 | 0.0087 | 0.0034 | 0.0028 | 0.02 | 0.0018 | 0.0187 | 0.0005 | 0.0071 | 0.0128 | 0.0292 | 0.0179 | 0.0007 | 0.0012 | 0.0008 | 0.0004 | 0.0102 | 0.004 |
Vacuole | 0.0017 | 0.0005 | 0.0006 | 0.002 | 0.0003 | 0.0002 | 0.0046 | 0 | 0.0016 | 0.0006 | 0.0021 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Vacuole Periphery | 0.0012 | 0.0003 | 0.0005 | 0.0015 | 0.0002 | 0.0001 | 0.0022 | 0 | 0.0009 | 0.0004 | 0.0019 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.37 | 14.744 | 10.9407 | 17.3368 | 24.592 | 18.1326 | 9.69 | 16.6018 | 18.3824 | 13.9422 |
Translational Efficiency | 2.2456 | 1.8285 | 1.9941 | 1.2275 | 1.243 | 1.4693 | 2.4028 | 1.5536 | 1.214 | 1.8468 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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