Standard name
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.08 0.15 0.15 0.1 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.09 0 0.15 0.06 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.87 0.89 0.93 0.87 0.82 0.82 0.74 0.74 0.74 0.74 0.84 0.75 0.77 0.78 0.84 0.79 0.76 0.84 0.76 0.63 0.6 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.27 0.13 0.13 0.07 0.11 0.14 0.17 0.31 0.32 0.26 0.32 0.19 0.16 0.12 0.12 0.07 0.11 0.17 0.07 0.18 0.29 0.29 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1
Bud 0 1 0 0 0 0 1 1 2 4 6 0 0 1 0 0 0 3 1 2 1 5 6
Bud Neck 0 0 0 0 0 0 2 1 2 1 6 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 1 0 0 0 4 7 3 20 13 20 21 0 2 1 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 5 8 11 14 17 16 18 11 16 12 9 28 76 94 18 21 24 0 2 0 0 1 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 3 0 0 0 0 2 0
Endosome 2 2 5 0 5 2 4 1 6 5 1 1 4 7 1 0 0 1 5 4 4 3 9
Golgi 0 0 0 1 0 0 0 0 0 0 0 1 0 1 1 2 0 3 2 2 2 3 4
Mitochondria 1 21 1 5 6 40 10 103 38 91 79 0 1 1 7 3 9 0 3 1 1 3 7
Nucleus 312 321 376 387 355 375 556 516 510 428 599 291 387 494 141 168 164 282 345 353 256 346 356
Nuclear Periphery 1 0 4 3 1 5 6 8 5 3 7 1 3 2 0 1 0 0 0 0 0 2 1
Nucleolus 95 48 55 27 43 64 116 217 220 152 258 65 81 75 22 14 22 64 30 82 120 167 142
Peroxisomes 1 0 0 0 0 0 0 0 1 0 0 0 5 5 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 3 1 5 4 21 2 15 8 9 1 6 15 4 2 2 1 2 3 4 6 12
Vac/Vac Membrane 2 9 2 2 9 11 10 3 5 4 6 2 6 5 4 4 2 11 13 7 14 31 36
Unique Cell Count 352 370 421 414 407 455 675 693 689 578 813 346 515 643 181 200 207 373 410 463 409 581 589
Labelled Cell Count 420 411 457 440 446 524 747 883 833 728 1001 390 571 702 201 216 227 373 410 463 409 581 589


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 7.6 8.7 8.4 8.3 6.4 7.1 6.2 6.6 6.3 6.3 9.1 8.7 9.0 9.2 10.4 8.6 8.0 8.4 8.7
Std Deviation (1e-4) 1.3 1.4 1.7 1.8 1.7 1.8 1.6 1.3 1.3 1.4 1.3 2.8 2.5 2.6 2.3 2.1 2.0 2.0 1.9 2.2
Intensity Change (Log2) -0.06 -0.08 -0.44 -0.29 -0.49 -0.41 -0.47 -0.47 0.07 -0.0 0.04 0.07 0.25 -0.01 -0.12 -0.06 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3.5 0 3.9 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 1.2 0.8 0.1 -1.2 -0.3 -0.6 -2.0 3.4 6.4 6.4 3.9 4.2 4.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.0 0 8.1 4.6 8.4 6.4 0 0 0 0 0 0
Nucleus 2.1 -0.9 -2.9 -3.1 -6.0 -6.2 -6.0 -6.4 -2.1 -5.6 -5.2 -3.7 -1.9 -3.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.2 -1.1 0.4 1.8 6.9 7.1 5.1 7.1 2.2 1.2 -0.7 -0.3 -2.2 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2.6 0 1.9 0 0 0 0 2.0 0 0 0
Vacuole 0 2.2 2.4 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Med7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available