Standard name
Human Ortholog
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0 0 0.05 0 0 0 0 0 0.06 0.11 0.2 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 – 0 0 0 0.07 0.05 0.2 0.12 0.27 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.96 – 0.95 0.93 0.96 0.9 0.91 0.89 0.88 0.86 0.82 0.94 0.86 0.71 0.89 0.9 0.88 0.9 0.93 0.84 0.75 0.78 0.65
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0.09 0 0.05 0.08 0 0.08 0.05 0.05 0.05 0.06 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.08 0.11 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 1 0 – 0 1 0 0 1 1 3 1 1 0 0 0 0 0 1 0 3 1 0 1 2
Bud Neck 0 0 – 0 0 0 0 1 0 3 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 1 0 2 4 2 7 13 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 6 – 9 12 6 22 21 9 17 12 9 3 20 46 10 6 13 1 0 0 1 1 4
Endoplasmic Reticulum 1 1 – 0 0 0 0 0 0 0 0 0 0 0 0 3 2 3 0 1 0 0 1 1
Endosome 0 0 – 0 2 0 2 1 0 0 0 0 0 0 3 1 0 0 1 1 0 0 1 1
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 2 0
Mitochondria 21 1 – 1 0 11 35 31 50 49 75 64 0 0 1 8 4 13 2 0 0 0 1 3
Nucleus 159 240 – 203 284 254 431 562 223 365 235 300 49 154 165 268 204 287 154 235 32 27 115 120
Nuclear Periphery 0 0 – 1 0 1 7 4 1 4 1 5 0 2 4 1 5 3 0 0 0 0 0 0
Nucleolus 4 1 – 0 3 2 9 13 23 15 13 31 1 14 12 16 11 18 1 2 0 0 5 7
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 2 5 1 7 0 4 0 0 5 0 0 3 0 0 0 1 1 0
Vac/Vac Membrane 0 5 – 1 9 4 7 17 1 9 4 6 1 2 8 12 7 15 4 5 1 2 15 41
Unique Cell Count 169 251 213 306 265 477 617 250 414 274 366 52 180 231 302 227 327 171 253 39 37 149 185
Labelled Cell Count 190 254 215 311 279 515 659 313 475 348 434 54 193 248 321 240 357 171 253 39 37 149 185


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 8.0 – 7.6 7.8 6.5 6.2 6.5 5.2 5.5 5.3 5.4 8.3 8.0 7.9 10.8 11.0 9.7 – 7.5 8.1
Std Deviation (1e-4) 0.8 1.6 – 1.6 1.6 2.6 1.6 1.5 1.1 1.3 1.4 1.6 1.5 1.6 1.8 2.7 2.8 2.6 – 1.4 1.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9512 4.3079 3.802 3.4633 3.4399 3.771 3.5708 4.0713 3.8116 3.4349 3.3925 3.7495 1.669 2.5473 1.8128 1.8388 1.4578 1.5067
Actin 0.0134 0.0001 0.0123 0.0006 0.0423 0.0027 0.0418 0 0.0246 0.0017 0.079 0.017 0.0139 0.0099 0.0069 0.0097 0.0006 0
Bud 0.0008 0.0001 0.0004 0.0006 0.0007 0.0002 0.0007 0 0.0005 0.0002 0.0027 0.0001 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001
Bud Neck 0.0029 0.0003 0.0052 0.0011 0.0011 0.001 0.002 0.0001 0.0014 0.0007 0.0098 0.0026 0.0013 0.0001 0.0002 0.0003 0.0008 0.0008
Bud Periphery 0.0023 0.0003 0.0006 0.0023 0.0008 0.0004 0.0012 0 0.001 0.0002 0.0038 0.0005 0.0012 0.0001 0.0003 0.0003 0.0009 0.0002
Bud Site 0.0029 0.0006 0.0025 0.0007 0.0083 0.0001 0.0102 0.0001 0.0063 0.004 0.0215 0.0003 0.0019 0.0016 0.0004 0.0004 0.001 0.0001
Cell Periphery 0.0013 0.0001 0.0002 0.0005 0.0002 0.0001 0.0006 0.0001 0.001 0.0001 0.0005 0.0004 0.0007 0.0001 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.0026 0.0001 0.0033 0.0003 0.0006 0.0002 0.002 0.0001 0.0027 0.0001 0.0038 0.0041 0.0028 0 0.0013 0.0003 0.0005 0
Cytoplasmic Foci 0.0092 0.0002 0.0082 0.0003 0.0154 0.0001 0.0079 0 0.0045 0.0103 0.0303 0.0029 0.0121 0 0.0014 0.0142 0.0021 0
Eisosomes 0.0003 0 0.0001 0.0001 0.0003 0 0.0005 0 0.0003 0 0.0005 0.0001 0.0004 0 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0039 0.0001 0.0032 0.0007 0.0008 0.0009 0.0022 0 0.0023 0 0.0033 0.0009 0.0016 0 0.001 0.0004 0.0006 0
Endosome 0.0282 0.0005 0.0181 0.0023 0.0148 0.0013 0.0134 0 0.0033 0.0019 0.0166 0.0046 0.0066 0 0.0031 0.0282 0.0052 0
Golgi 0.0084 0.0001 0.0086 0.0004 0.0178 0.0007 0.0059 0 0.0036 0.004 0.0153 0.0027 0.0029 0 0.0005 0.0206 0.0009 0
Lipid Particles 0.0154 0.0005 0.0089 0.006 0.0154 0.0001 0.0184 0 0.0044 0.021 0.0103 0.0019 0.0102 0 0.0046 0.018 0.0042 0
Mitochondria 0.0152 0.0039 0.0052 0.018 0.0089 0.0029 0.0127 0.0002 0.0061 0.0012 0.006 0.007 0.006 0.0004 0.0013 0.007 0.0045 0.0002
None 0.0023 0.0001 0.0017 0.0004 0.0005 0.0001 0.0037 0 0.0031 0.0002 0.0065 0.0005 0.0149 0 0.0008 0.0012 0.0005 0
Nuclear Periphery 0.0221 0.0032 0.0223 0.0103 0.0055 0.0053 0.0072 0.0039 0.0064 0.0007 0.0091 0.0034 0.0048 0.0005 0.0097 0.0025 0.0107 0.0042
Nucleolus 0.0894 0.0681 0.0893 0.1508 0.0757 0.1046 0.0743 0.0869 0.0869 0.124 0.0546 0.1382 0.0722 0.0701 0.1244 0.1467 0.0975 0.1138
Nucleus 0.7568 0.9117 0.7856 0.7832 0.7787 0.8757 0.7781 0.9067 0.8287 0.7708 0.6664 0.808 0.8322 0.9153 0.8339 0.7253 0.8453 0.8796
Peroxisomes 0.0029 0.0001 0.0041 0.0003 0.0077 0 0.0088 0 0.0046 0.0443 0.0443 0.0013 0.0071 0 0.0002 0.0157 0.0004 0
Punctate Nuclear 0.0063 0.0081 0.0161 0.0091 0.0029 0.0027 0.0033 0.0014 0.0026 0.014 0.0128 0.0008 0.0035 0.001 0.0063 0.0073 0.0189 0.0003
Vacuole 0.0086 0.0011 0.0026 0.0063 0.0011 0.0004 0.0026 0.0002 0.0044 0.0004 0.002 0.0021 0.0022 0.0004 0.0021 0.0008 0.0031 0.0003
Vacuole Periphery 0.0049 0.0008 0.0016 0.0056 0.0007 0.0004 0.0024 0.0001 0.0014 0.0001 0.0011 0.0008 0.0012 0.0003 0.0012 0.0008 0.0019 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.0621 22.4815 21.6869 19.3179 26.4928 26.3916 20.7421 26.273 23.1241 23.7658
Translational Efficiency 0.9802 0.8662 0.8168 0.7052 0.615 0.6466 0.861 0.6247 0.6107 0.6904

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1836 1450 143 148 1498 1828 304 1311 3334 3278 447 1459

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 727.13 792.65 1178.63 1111.87 770.45 814.87 1071.57 987.00 746.59 805.04 1105.82 999.67
Standard Deviation 94.95 129.64 133.44 167.52 108.34 117.17 137.57 149.69 103.46 123.34 145.13 156.21
Intensity Change Log 2 — 0.124471 0.696826 0.612703 — 0.080869 0.475953 0.357349 — 0.102204 0.587420 0.486980

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000127 0.000465 0.001560 0.000903 0.000319 0.000402 0.000967 0.000738 0.000213 0.000430 0.001157 0.000755
Bud Neck 0.002899 0.006139 0.010657 0.032180 0.004833 0.006927 0.014512 0.022563 0.003768 0.006579 0.013279 0.023538
Bud Site 0.002207 0.000753 0.001206 0.001388 0.000500 0.000760 0.001086 0.001407 0.001440 0.000757 0.001124 0.001405
Cell Periphery 0.000136 0.000174 0.000056 0.000072 0.000094 0.000126 0.000049 0.000059 0.000117 0.000147 0.000051 0.000061
Cytoplasm 0.004642 0.002000 0.000411 0.000687 0.002951 0.001234 0.000672 0.000990 0.003882 0.001573 0.000588 0.000959
Cytoplasmic Foci 0.000904 0.000515 0.000046 0.000019 0.000331 0.000390 0.000038 0.000131 0.000647 0.000446 0.000041 0.000120
Eisosomes 0.000013 0.000023 0.000056 0.000034 0.000020 0.000022 0.000040 0.000027 0.000016 0.000023 0.000045 0.000027
Endoplasmic Reticulum 0.003892 0.008198 0.006776 0.003085 0.004155 0.008060 0.005266 0.004372 0.004010 0.008121 0.005749 0.004241
Endosome 0.000380 0.000439 0.000196 0.000143 0.000426 0.000868 0.000116 0.000519 0.000401 0.000678 0.000142 0.000481
Golgi 0.000124 0.000104 0.000096 0.000073 0.000156 0.000502 0.000082 0.000232 0.000139 0.000326 0.000087 0.000216
Lipid Particles 0.000158 0.000198 0.000202 0.000048 0.000175 0.000229 0.000101 0.000055 0.000165 0.000215 0.000133 0.000054
Mitochondria 0.001449 0.001612 0.001423 0.001528 0.001267 0.002125 0.001080 0.001538 0.001367 0.001898 0.001190 0.001537
Mitotic Spindle 0.000787 0.000260 0.000093 0.003451 0.000828 0.002914 0.000561 0.008120 0.000806 0.001740 0.000412 0.007646
None 0.005522 0.003170 0.000577 0.006323 0.005684 0.001487 0.001754 0.001619 0.005595 0.002232 0.001378 0.002096
Nuclear Periphery 0.000681 0.000606 0.000305 0.000350 0.000475 0.001336 0.000220 0.000840 0.000588 0.001013 0.000247 0.000791
Nuclear Periphery Foci 0.000287 0.000238 0.000113 0.000040 0.001026 0.000179 0.000058 0.000434 0.000619 0.000205 0.000076 0.000394
Nucleolus 0.011606 0.017155 0.014740 0.034797 0.047198 0.014507 0.025264 0.026571 0.027598 0.015678 0.021897 0.027406
Nucleus 0.962670* 0.956646* 0.957393* 0.913100* 0.927736* 0.954335* 0.946167* 0.925945* 0.946973* 0.955357* 0.949758* 0.924642*
Peroxisomes 0.000179 0.000394 0.000783 0.000351 0.000324 0.000437 0.000600 0.000250 0.000244 0.000418 0.000659 0.000260
Vacuole 0.001018 0.000694 0.003153 0.001216 0.000855 0.001879 0.001210 0.002009 0.000945 0.001355 0.001832 0.001929
Vacuole Periphery 0.000319 0.000217 0.000158 0.000211 0.000648 0.001279 0.000156 0.001580 0.000467 0.000809 0.000157 0.001441

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.90 -10.80 -10.44 -6.95 3.53 -0.58 -10.35 -5.74 -2.85 1.69 -2.71 -15.19 -7.86 -4.09 3.58
Bud Neck -5.29 -5.58 -7.73 -7.08 -5.48 -2.82 -6.11 -15.79 -14.03 -5.66 -5.67 -8.08 -18.17 -16.13 -7.95
Bud Site 1.72 0.77 -0.85 -2.92 -1.97 -1.17 -5.20 -6.06 -4.14 -2.50 1.40 -0.09 -1.71 -5.24 -2.97
Cell Periphery -0.92 3.18 1.88 2.27 -1.23 -0.86 2.29 1.76 1.91 -0.83 -1.08 4.07 3.37 3.31 -0.83
Cytoplasm 2.43 4.61 0.93 -0.32 -1.22 2.54 1.18 2.87 0.22 0.34 3.55 2.92 4.08 0.73 -0.16
Cytoplasmic Foci 0.52 2.55 1.52 1.07 -1.02 -0.17 2.12 1.57 2.01 -1.56 0.59 3.13 2.62 2.56 -1.80
Eisosomes -6.99 -11.45 -8.12 -4.77 3.61 -1.46 -8.76 -7.14 -4.73 5.20 -6.26 -14.42 -12.87 -5.91 7.70
Endoplasmic Reticulum -8.70 -4.63 0.39 8.45 4.72 -7.41 -1.34 -1.10 7.10 0.94 -11.05 -3.27 -1.54 10.46 2.65
Endosome -0.83 1.41 1.41 2.54 -0.09 -1.84 2.74 -0.28 1.51 -2.33 -2.07 3.27 -0.37 1.34 -2.16
Golgi -0.00 0.12 -0.80 -1.08 -1.42 -1.42 1.08 -0.69 1.08 -1.25 -1.41 0.64 -0.95 0.71 -1.34
Lipid Particles -0.88 -1.51 0.42 1.13 1.86 -0.61 1.90 3.06 2.06 1.63 -1.08 0.40 2.98 2.67 3.33
Mitochondria -0.20 -0.26 -1.13 -0.72 -2.45 -1.27 0.14 -1.04 0.70 -4.46 -1.02 0.13 -1.20 0.22 -4.20
Mitotic Spindle 1.64 2.04 -2.05 -2.11 -2.12 -1.81 -1.28 -3.86 -2.78 -2.24 -1.46 -1.15 -4.38 -3.87 -3.25
None 2.29 5.26 0.29 -0.55 -1.50 4.70 2.56 4.46 -0.53 0.84 4.88 4.15 4.76 0.44 -0.17
Nuclear Periphery 0.60 2.58 -0.94 -1.35 -2.47 -3.93 2.71 -3.65 1.50 -5.09 -3.09 3.79 -2.90 0.31 -5.34
Nuclear Periphery Foci -0.44 1.03 1.10 1.35 0.13 2.20 2.37 1.45 -1.44 -1.81 1.83 2.65 0.95 -0.80 -1.74
Nucleolus -3.80 -1.33 -3.86 -3.18 -3.11 12.61* 1.97 2.56 -7.86 -0.39 8.45 -0.37 -3.63 -8.23 -1.93
Nucleus 1.87 2.13 5.85 5.44 4.93 -6.24 0.00 4.65 9.72 3.08 -2.95 2.09 9.75 11.63 5.36
Peroxisomes -2.39 -5.98 -3.51 0.19 3.27 -0.64 -2.05 0.04 0.68 2.11 -2.00 -3.67 -2.05 1.10 3.07
Vacuole 1.20 -2.39 -2.19 -2.56 0.54 -2.42 -1.98 -3.41 -1.47 -2.27 -1.56 -2.94 -3.68 -2.79 -0.86
Vacuole Periphery 1.15 1.74 0.55 -0.87 -1.95 -1.35 2.50 -1.08 -0.00 -2.03 -1.32 2.99 -1.41 -0.68 -2.05
Standard name
Human Ortholog
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pap2

Pap2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pap2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available