Standard name
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.11 0.2 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.07 0.05 0.2 0.12 0.27 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.96 0.95 0.93 0.96 0.9 0.91 0.89 0.88 0.86 0.82 0.94 0.86 0.71 0.89 0.9 0.88 0.9 0.93 0.84 0.75 0.78 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.09 0 0.05 0.08 0 0.08 0.05 0.05 0.05 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.08 0.11 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 1 0 0 1 0 0 1 1 3 1 1 0 0 0 0 0 1 0 3 1 0 1 2
Bud Neck 0 0 0 0 0 0 1 0 3 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 2 4 2 7 13 0 1 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 6 9 12 6 22 21 9 17 12 9 3 20 46 10 6 13 1 0 0 1 1 4
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 0 0 0 3 2 3 0 1 0 0 1 1
Endosome 0 0 0 2 0 2 1 0 0 0 0 0 0 3 1 0 0 1 1 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 2 0
Mitochondria 21 1 1 0 11 35 31 50 49 75 64 0 0 1 8 4 13 2 0 0 0 1 3
Nucleus 159 240 203 284 254 431 562 223 365 235 300 49 154 165 268 204 287 154 235 32 27 115 120
Nuclear Periphery 0 0 1 0 1 7 4 1 4 1 5 0 2 4 1 5 3 0 0 0 0 0 0
Nucleolus 4 1 0 3 2 9 13 23 15 13 31 1 14 12 16 11 18 1 2 0 0 5 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 5 1 7 0 4 0 0 5 0 0 3 0 0 0 1 1 0
Vac/Vac Membrane 0 5 1 9 4 7 17 1 9 4 6 1 2 8 12 7 15 4 5 1 2 15 41
Unique Cell Count 169 251 213 306 265 477 617 250 414 274 366 52 180 231 302 227 327 171 253 39 37 149 185
Labelled Cell Count 190 254 215 311 279 515 659 313 475 348 434 54 193 248 321 240 357 171 253 39 37 149 185


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 8.0 7.6 7.8 6.5 6.2 6.5 5.2 5.5 5.3 5.4 8.3 8.0 7.9 10.8 11.0 9.7 7.5 8.1
Std Deviation (1e-4) 0.8 1.6 1.6 1.6 2.6 1.6 1.5 1.1 1.3 1.4 1.6 1.5 1.6 1.8 2.7 2.8 2.6 1.4 1.2
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pap2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available