Standard name
Human Ortholog
Description Myo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.17 0.21 0.12 0.15 0.14 0.1 0.13 0.11 0.13 0.16 0.08 0.05 0.05 0.05 – 0.08 0.05 0.11 0.08 0.1 0.09 0.07 0.06
Bud 0 0.06 0.05 0.07 0 0 0 0.1 0.06 0.06 0.06 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0.07 0 0.05 0.05 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0.36 0.54 0.49 0.43 0.42 0.35 0.33 0.3 0.34 0.37 0.32 0.4 0.55 0.55 0.41 – 0.36 0.36 0.6 0.7 0.76 0.65 0.68 0.67
Cytoplasm 0.68 0.55 0.46 0.5 0.51 0.47 0.47 0.42 0.4 0.39 0.34 0.35 0.62 0.62 0.76 – 0.67 0.66 0.1 0.07 0 0.08 0.05 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.08 0.05 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.05 0.12 0.24 0.22 0.46 0.41 0.46 0.54 0.55 0.57 0.57 0 0 0 – 0 0.09 0 0 0 0.05 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 63 69 26 37 32 33 39 36 57 55 39 37 39 34 – 19 8 29 35 39 24 25 22
Bud 4 21 17 15 9 9 14 30 20 28 19 23 13 13 9 – 2 3 3 2 0 0 3 7
Bud Neck 1 0 3 0 0 0 0 1 0 0 0 0 8 8 1 – 3 6 14 29 12 13 18 19
Bud Site 0 0 1 0 1 1 0 2 0 0 0 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 77 201 158 92 103 82 113 92 114 163 110 190 413 413 292 – 90 57 154 307 288 175 235 241
Cytoplasm 144 205 147 107 123 110 160 127 133 169 117 164 470 470 542 – 166 104 25 29 6 23 18 27
Endoplasmic Reticulum 2 4 2 4 3 5 7 5 3 6 14 11 11 7 5 – 18 13 13 11 12 9 8 14
Endosome 1 0 0 0 1 0 1 1 1 0 0 1 1 1 0 – 2 2 2 1 2 1 1 2
Golgi 3 2 1 0 0 0 0 0 0 0 0 0 0 0 0 – 1 1 2 2 0 1 0 0
Mitochondria 10 18 39 51 53 108 140 139 181 240 194 269 0 0 0 – 11 14 7 11 8 14 20 12
Nucleus 0 0 0 0 0 0 0 2 2 2 1 1 0 0 1 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 1 2 1 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 12 16 16 8 11 5 5 9 1 10 0 0 0 – 0 2 0 0 1 0 0 0
SpindlePole 2 2 16 5 5 0 4 4 0 2 3 1 13 12 4 – 12 3 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 3 2 0 1 2 1 1 1 1 0 0 2 – 2 0 2 6 1 4 8 4
Unique Cell Count 213 373 323 214 243 235 340 304 335 438 341 474 755 754 716 249 157 259 440 378 271 345 360
Labelled Cell Count 267 516 465 319 354 356 485 451 499 678 516 712 966 963 890 326 213 259 440 378 271 345 360


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.8 5.3 5.2 5.5 4.9 5.1 5.3 5.2 4.9 5.1 5.2 6.7 6.7 6.8 4.8 5.6 6.5 6.2 6.4 6.8
Std Deviation (1e-4) 0.8 0.7 1.1 1.2 1.5 1.1 1.3 1.1 1.0 0.9 1.1 0.7 0.8 0.8 0.9 1.0 1.2 2.2 1.0 1.3 1.5
Intensity Change (Log2) – – – -0.04 0.05 -0.11 -0.04 0.0 -0.04 -0.1 -0.06 -0.02 0.34 0.34 0.36 -0.13 0.07 0.31 0.23 0.27 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.7 -1.9 -2.3 -4.2 -2.8 -3.7 -3.1 -1.7 -5.3 -8.3 -8.1 -8.3 – -4.5 -4.6
Bud 0.8 -0.9 -0.8 -0.7 2.2 0.4 0.7 0.2 -0.3 -3.2 -3.2 -3.8 – -3.0 -1.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery -1.3 -1.5 -3.3 -4.1 -4.8 -3.9 -3.2 -4.4 -2.5 1.7 1.8 -2.4 – -3.1 -2.6
Cytoplasm 1.0 1.2 0.3 0.4 -0.9 -1.5 -1.9 -2.9 -3.1 5.1 5.1 9.5 – 5.0 4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 2.9 1.9 0 0 0 – 4.3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 3.6 3.1 9.0 8.4 9.3 11.4 12.1 12.1 12.7 -9.7 -9.7 -9.5 – -3.2 -1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 1.9 1.6 -0.2 -0.3 -1.6 -1.8 -1.4 -3.2 -1.4 0 0 0 – -3.1 0
SpindlePole -1.5 -1.8 -3.5 -2.8 -2.6 -4.1 -4.0 -3.1 -4.5 -3.0 -3.2 -4.8 – -0.1 -1.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8583 3.3442 3.1859 3.0275 2.9249 3.1554 3.5042 4.7827 4.833 3.9339 4.7445 4.5247 4.643 4.8481 4.068 5.1305 4.365 4.1476
Actin 0.0518 0.007 0.0162 0.0011 0.025 0.004 0.0157 0.0061 0.0222 0.0001 0.0021 0.0003 0.1388 0.0017 0.0122 0.0004 0.0691 0.0156
Bud 0.012 0.0009 0.0102 0.0007 0.0013 0.0022 0.0011 0.0004 0.0007 0.0002 0.0003 0.0003 0.001 0.0005 0.0038 0.0002 0.0006 0.001
Bud Neck 0.0092 0.0043 0.0187 0.014 0.0044 0.0067 0.017 0.0034 0.0063 0.0015 0.0065 0.003 0.0068 0.0056 0.0116 0.0014 0.0018 0.01
Bud Periphery 0.0307 0.0067 0.014 0.0145 0.0259 0.0127 0.0061 0.0033 0.0066 0.0046 0.0067 0.0059 0.0029 0.0022 0.0322 0.0035 0.0021 0.0108
Bud Site 0.0236 0.0729 0.0615 0.0062 0.0036 0.0043 0.0246 0.0903 0.0257 0.0009 0.0036 0.001 0.0058 0.0645 0.0589 0.0016 0.0046 0.0036
Cell Periphery 0.4019 0.3918 0.4425 0.6161 0.572 0.5359 0.593 0.4598 0.4726 0.7106 0.4463 0.6279 0.1637 0.3432 0.497 0.3075 0.6641 0.4503
Cytoplasm 0.2584 0.4563 0.3631 0.3268 0.3126 0.3673 0.2403 0.3371 0.3284 0.2788 0.3135 0.31 0.3847 0.4564 0.273 0.6002 0.1847 0.4091
Cytoplasmic Foci 0.012 0.0016 0.0046 0.0008 0.0039 0.0032 0.0027 0.0012 0.0014 0 0.0012 0.0003 0.04 0.0029 0.005 0.0016 0.0181 0.0028
Eisosomes 0.0055 0.0073 0.0042 0.0014 0.0021 0.0013 0.0117 0.0042 0.0071 0.0003 0.0073 0.0007 0.003 0.0008 0.0022 0.0005 0.0003 0.0011
Endoplasmic Reticulum 0.0146 0.0103 0.0102 0.0073 0.0127 0.0094 0.003 0.0028 0.0036 0.0014 0.0111 0.0016 0.0301 0.005 0.0077 0.009 0.0024 0.0033
Endosome 0.019 0.0003 0.0046 0.0001 0.0044 0.0008 0.0011 0.0001 0.0002 0 0.0001 0 0.0644 0.0097 0.0408 0.0004 0.0092 0.0193
Golgi 0.0057 0.001 0.0036 0.0002 0.0091 0.001 0.0011 0.0003 0.0007 0 0.0004 0.0001 0.0135 0.0011 0.0015 0.0002 0.0166 0.0014
Lipid Particles 0.0067 0.0042 0.004 0.0005 0.0116 0.0054 0.002 0.0015 0.0008 0.0001 0.0014 0.0004 0.0072 0.0015 0.0016 0.0016 0.0067 0.0021
Mitochondria 0.0053 0.0003 0.003 0.0002 0.0016 0.0002 0.0002 0 0.0002 0 0.0002 0 0.0045 0.001 0.0009 0.0001 0.003 0.0002
None 0.1054 0.0323 0.028 0.0066 0.0015 0.0363 0.0752 0.087 0.1199 0.0004 0.1943 0.0456 0.0216 0.0758 0.0046 0.0653 0.0027 0.0388
Nuclear Periphery 0.0096 0.0003 0.0014 0.0002 0.0006 0.0003 0.0003 0.0002 0.0002 0.0001 0.0003 0.0001 0.009 0.0013 0.0006 0.0003 0.0003 0.0005
Nucleolus 0.0017 0.0002 0.0006 0.0001 0.0001 0.0003 0.0003 0.0002 0.0004 0 0.0006 0 0.0009 0.0005 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0079 0.0004 0.0016 0.0003 0.0003 0.0005 0.0003 0.0002 0.0004 0 0.0007 0.0001 0.0026 0.0012 0.0007 0.0004 0.0002 0.0005
Peroxisomes 0.0025 0.0001 0.0016 0 0.0031 0.0036 0.0003 0 0.0005 0 0 0 0.0027 0.0001 0.0002 0 0.0106 0.0001
Punctate Nuclear 0.0057 0.0001 0.0023 0 0.0005 0.0004 0.0003 0.0001 0.0001 0 0.0001 0 0.0298 0.0002 0.0001 0.0001 0.0009 0.0002
Vacuole 0.0096 0.0018 0.0041 0.0029 0.0034 0.0041 0.0036 0.0016 0.0019 0.0011 0.0031 0.0027 0.0605 0.0242 0.0395 0.0056 0.0016 0.0204
Vacuole Periphery 0.0012 0 0.0003 0 0.0002 0.0001 0.0001 0 0 0 0 0 0.0063 0.0007 0.0054 0 0.0001 0.0086

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.6628 40.9643 36.3967 45.0153 50.4232 42.1834 42.6451 46.7142 38.6887 52.0269
Translational Efficiency 0.4501 0.4918 0.4818 0.3399 0.3447 0.4658 0.4366 0.3209 0.3595 0.328

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Myo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (28%), cytoplasm (3%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Itr2

Itr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Itr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available