Standard name
Description Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0.1 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0.06 0 0 0.06 0 0 0 – 0.05 0.07 0.08 0.08 0.08 0 0 0 0 0.07 0.18 0 0 0
Cytoplasm 0.94 0.98 0.97 0.99 0.95 0.88 0.85 0.83 0.72 – 0.62 0.5 0.97 0.98 0.98 0.94 0.93 0.97 0.64 0.77 0.46 0.9 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.22 0.23 0.26 0.42 – 0.49 0.63 0 0 0 0.05 0 0 0.09 0 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 0 1 1 0 1 2 2 – 3 3 3 3 2 0 0 1 16 16 21 2 1 3
Bud 2 1 5 3 3 10 7 11 8 – 16 14 2 3 10 0 0 0 1 0 1 1 2 8
Bud Neck 1 1 5 1 0 1 4 3 0 – 0 1 0 3 0 6 4 4 6 11 8 0 0 2
Bud Site 0 0 0 0 0 2 0 1 2 – 4 0 0 0 1 0 0 0 – – – – – –
Cell Periphery 6 25 14 6 9 16 7 7 13 – 15 22 17 22 27 6 9 10 9 25 38 2 1 2
Cytoplasm 190 336 218 197 257 234 365 463 246 – 200 151 202 262 317 346 230 387 144 272 97 136 168 252
Endoplasmic Reticulum 5 2 0 0 1 0 0 0 3 – 2 8 2 1 2 39 9 23 8 3 4 1 0 3
Endosome 1 0 0 0 1 1 1 0 0 – 0 0 0 0 1 4 5 6 7 2 2 0 0 2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 5 0 1 4 1 2 1 0 3
Mitochondria 0 5 1 7 17 60 101 147 144 – 157 191 0 0 9 17 4 4 20 12 28 2 3 5
Nucleus 0 0 0 0 0 2 3 4 0 – 1 2 0 0 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 0 1 1 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 3 5 0 5 – 7 3 0 1 0 1 0 0 1 0 0 0 0 0
SpindlePole 0 1 1 0 2 4 8 14 10 – 6 3 0 2 0 2 3 1 1 0 0 0 0 1
Vac/Vac Membrane 2 0 1 0 0 1 1 0 0 – 0 0 0 0 0 9 8 4 3 5 3 1 1 2
Unique Cell Count 202 343 224 199 271 267 430 557 340 320 304 209 268 325 370 246 399 226 355 215 151 185 291
Labelled Cell Count 210 372 245 216 293 334 504 653 434 412 398 226 297 369 437 274 441 226 355 215 151 185 291


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.2 4.0 3.8 4.0 3.7 3.5 3.6 3.2 – 3.3 3.0 5.0 4.9 4.2 5.9 6.7 6.4 4.9 4.9 5.4
Std Deviation (1e-4) 0.6 0.7 0.9 1.3 1.2 1.2 1.2 1.3 0.8 – 1.2 0.7 1.2 1.4 1.2 2.3 3.2 2.8 1.1 1.4 1.4
Intensity Change (Log2) – – – -0.09 -0.0 -0.14 -0.19 -0.14 -0.34 – -0.29 -0.44 0.33 0.29 0.08 0.55 0.73 0.66 0.3 0.3 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pkh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available