Standard name | |
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Description | Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 2 | – | 3 | 3 | 3 | 3 | 2 | 0 | 0 | 1 | 16 | 16 | 21 | 2 | 1 | 3 |
Bud | 2 | 1 | 5 | 3 | 3 | 10 | 7 | 11 | 8 | – | 16 | 14 | 2 | 3 | 10 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 8 |
Bud Neck | 1 | 1 | 5 | 1 | 0 | 1 | 4 | 3 | 0 | – | 0 | 1 | 0 | 3 | 0 | 6 | 4 | 4 | 6 | 11 | 8 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | – | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 25 | 14 | 6 | 9 | 16 | 7 | 7 | 13 | – | 15 | 22 | 17 | 22 | 27 | 6 | 9 | 10 | 9 | 25 | 38 | 2 | 1 | 2 |
Cytoplasm | 190 | 336 | 218 | 197 | 257 | 234 | 365 | 463 | 246 | – | 200 | 151 | 202 | 262 | 317 | 346 | 230 | 387 | 144 | 272 | 97 | 136 | 168 | 252 |
Endoplasmic Reticulum | 5 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | – | 2 | 8 | 2 | 1 | 2 | 39 | 9 | 23 | 8 | 3 | 4 | 1 | 0 | 3 |
Endosome | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 6 | 7 | 2 | 2 | 0 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 4 | 1 | 2 | 1 | 0 | 3 |
Mitochondria | 0 | 5 | 1 | 7 | 17 | 60 | 101 | 147 | 144 | – | 157 | 191 | 0 | 0 | 9 | 17 | 4 | 4 | 20 | 12 | 28 | 2 | 3 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 0 | – | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 0 | 5 | – | 7 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 0 | 2 | 4 | 8 | 14 | 10 | – | 6 | 3 | 0 | 2 | 0 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 9 | 8 | 4 | 3 | 5 | 3 | 1 | 1 | 2 |
Unique Cell Count | 202 | 343 | 224 | 199 | 271 | 267 | 430 | 557 | 340 | 320 | 304 | 209 | 268 | 325 | 370 | 246 | 399 | 226 | 355 | 215 | 151 | 185 | 291 | |
Labelled Cell Count | 210 | 372 | 245 | 216 | 293 | 334 | 504 | 653 | 434 | 412 | 398 | 226 | 297 | 369 | 437 | 274 | 441 | 226 | 355 | 215 | 151 | 185 | 291 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 5.2 | 4.2 | 4.0 | 3.8 | 4.0 | 3.7 | 3.5 | 3.6 | 3.2 | – | 3.3 | 3.0 | 5.0 | 4.9 | 4.2 | 5.9 | 6.7 | 6.4 | 4.9 | 4.9 | 5.4 |
Std Deviation (1e-4) | 0.6 | 0.7 | 0.9 | 1.3 | 1.2 | 1.2 | 1.2 | 1.3 | 0.8 | – | 1.2 | 0.7 | 1.2 | 1.4 | 1.2 | 2.3 | 3.2 | 2.8 | 1.1 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.0 | -0.14 | -0.19 | -0.14 | -0.34 | – | -0.29 | -0.44 | 0.33 | 0.29 | 0.08 | 0.55 | 0.73 | 0.66 | 0.3 | 0.3 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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