Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.07 0.05 0 0 0 0.07 0 0.08 0.19 0.22 0.09 0.11 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.14 0.25 0.2 0.4 0.29 0.49 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.94 0.94 0.94 0.88 0.84 0.85 0.82 0.83 0.73 0.81 0.9 0.77 0.67 0.8 0.82 0.84 0.94 0.91 0.93 0.73 0.67 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.06 0 0.1 0.08 0.09 0.11 0 0 0.05 0 0 0 0 0 0 0.07 0.06 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.05 0 0 0 0 0.11 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 1 0 1 6 6 2 1 4 2 1 0 0 1 0 1 3 1 0 2 5 2
Bud Neck 0 0 0 0 1 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 2 10 2 8 9 15 10 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 5 7 13 24 18 13 14 13 15 9 12 38 45 21 22 25 0 1 0 4 1 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 10 0 4 0 0 0 0 0 1
Endosome 1 0 2 0 0 0 0 0 0 0 0 0 0 0 3 4 1 1 0 2 1 0 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 0 1 0 0 1 0
Mitochondria 20 4 3 8 2 47 92 75 138 105 111 55 2 0 0 7 4 9 2 1 2 2 3 5
Nucleus 334 230 163 189 266 294 307 320 280 296 165 174 131 154 134 194 159 194 327 229 158 121 150 138
Nuclear Periphery 8 1 0 1 0 4 2 1 1 3 4 0 1 1 3 2 3 2 0 0 0 1 1 8
Nucleolus 11 1 4 0 3 8 21 13 34 28 20 24 0 3 10 5 3 2 2 3 1 10 12 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 1 0 3 0 3 1 1 1 1 1 3 0 0 2 1 0 0 2 1
Vac/Vac Membrane 7 6 1 2 1 2 3 3 6 6 1 4 1 3 16 19 9 8 10 8 4 18 37 37
Unique Cell Count 350 245 173 202 282 333 365 378 341 356 225 214 146 199 201 242 195 231 350 253 171 167 225 215
Labelled Cell Count 385 247 180 209 290 391 451 444 485 470 331 279 149 201 213 267 204 248 350 253 171 167 225 215


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 9.2 7.2 7.1 7.2 5.5 5.1 5.5 5.0 5.5 4.8 5.1 7.9 8.4 9.0 8.6 9.4 8.9 8.2 8.4 8.6
Std Deviation (1e-4) 1.5 2.0 1.6 1.8 2.0 1.8 1.5 1.4 1.7 1.9 1.9 1.5 2.0 3.2 4.7 2.4 2.4 2.6 2.5 2.9 2.7
Intensity Change (Log2) – – – -0.03 -0.01 -0.38 -0.49 -0.39 -0.53 -0.4 -0.6 -0.52 0.13 0.22 0.32 0.25 0.37 0.3 0.17 0.21 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.9 2.0 1.1 0.3 0.7 0.5 1.7 0.7 2.1 4.9 5.5 2.4 3.1 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 0 4.4 6.7 5.7 9.3 7.4 0 6.6 0 0 0 0 0 1.3
Nucleus -0.3 0 -2.1 -3.3 -3.2 -3.8 -3.5 -5.4 -3.8 -1.5 -4.6 -6.6 -4.1 -3.7 -3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1.8 0.7 3.1 2.5 0 3.4 0 0 1.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.4 3.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6987 2.6498 2.4211 2.134 1.3778 2.3895 2.7831 3.2077 2.812 2.0841 2.0031 2.8217 2.9268 2.8437 2.6049 2.0812 2.2334 2.6183
Actin 0.0027 0 0.001 0.0065 0.0196 0.0001 0.0059 0 0.0007 0.0002 0.0001 0.0011 0.0182 0 0.0001 0.0024 0 0
Bud 0.0002 0 0.0001 0.0007 0.0007 0.0001 0.0002 0 0.0001 0 0 0 0.0002 0 0.0003 0.0001 0 0
Bud Neck 0.0016 0.0001 0.0005 0.0008 0.0007 0.0015 0.0044 0.0001 0.0003 0.0002 0.0005 0.0003 0.0013 0.0001 0.0007 0.0003 0.0001 0.0005
Bud Periphery 0.0004 0 0.0003 0.0012 0.002 0.0003 0.0005 0 0.0002 0.0002 0.0001 0.0001 0.0008 0 0.0006 0.0001 0 0
Bud Site 0.0014 0 0.0005 0.0023 0.0022 0.0001 0.0036 0.0001 0.0001 0.0002 0.0001 0 0.0006 0.0001 0.0004 0.0002 0 0
Cell Periphery 0.0003 0 0.0001 0.0003 0.0001 0.0001 0.0004 0 0.0001 0.0002 0 0 0.0005 0 0.0001 0 0 0
Cytoplasm 0.0079 0.0001 0.0004 0.0195 0.004 0.0003 0.0014 0.0007 0.0016 0.001 0.0014 0.0002 0.0012 0.0024 0.0013 0.0029 0.0001 0
Cytoplasmic Foci 0.004 0 0.0073 0.0039 0.0021 0.0002 0.0047 0.0002 0.0005 0.0007 0.0038 0.0001 0.0117 0 0 0.0064 0 0
Eisosomes 0 0 0 0.0001 0.0003 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0041 0 0.0003 0.002 0.0007 0.0003 0.0015 0.0001 0.0011 0.0009 0.0004 0.0002 0.0024 0.0002 0.0002 0.0011 0 0
Endosome 0.0163 0 0.0069 0.0074 0.0026 0.0009 0.0066 0.0002 0.002 0.0169 0.0146 0.0012 0.0051 0 0.0001 0.0176 0 0
Golgi 0.0015 0 0.0024 0.0044 0.0025 0.0001 0.0013 0 0.0002 0.001 0.0009 0.0003 0.0014 0 0 0.0019 0 0
Lipid Particles 0.0168 0 0.0073 0.0062 0.0139 0.0009 0.0074 0.0002 0.0002 0.0037 0.0041 0.001 0.0059 0 0 0.0088 0 0
Mitochondria 0.0017 0.0001 0.0046 0.0031 0.0034 0.0005 0.0016 0.0002 0.0012 0.0013 0.0003 0.0005 0.0091 0.0001 0.0006 0.0006 0.0005 0.0001
None 0.0025 0 0.0003 0.0304 0.042 0.0006 0.0011 0.0067 0.0008 0.0009 0.0119 0.001 0.001 0.0001 0.0001 0.0219 0 0
Nuclear Periphery 0.0105 0.0015 0.0172 0.0038 0.0255 0.0089 0.0114 0.0049 0.0044 0.0059 0.0043 0.0026 0.005 0.0025 0.0025 0.0204 0.0012 0.0004
Nucleolus 0.0172 0.0143 0.0122 0.0098 0.0353 0.0354 0.014 0.0131 0.0123 0.0159 0.0359 0.0276 0.0155 0.0122 0.0251 0.0084 0.0781 0.035
Nucleus 0.8874 0.9758 0.9175 0.8764 0.7957 0.944 0.9181 0.9584 0.9676 0.9419 0.8994 0.9576 0.9017 0.9714 0.9629 0.8851 0.9148 0.9522
Peroxisomes 0.0019 0 0.0086 0.002 0.0089 0 0.0017 0 0.0001 0.0002 0.0004 0.0001 0.0097 0 0 0.0011 0 0
Punctate Nuclear 0.0126 0.008 0.007 0.0144 0.036 0.0043 0.0114 0.0145 0.005 0.004 0.0206 0.0058 0.0043 0.0107 0.0035 0.0187 0.0049 0.0115
Vacuole 0.0075 0 0.0033 0.004 0.0008 0.0011 0.0016 0.0004 0.0007 0.0032 0.0007 0.0001 0.0025 0.0002 0.0009 0.0012 0.0001 0.0001
Vacuole Periphery 0.0014 0 0.002 0.0008 0.0011 0.0004 0.0012 0.0002 0.0007 0.0015 0.0003 0.0002 0.0016 0 0.0004 0.0007 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.3529 19.281 20.3315 25.5987 21.3414 26.0949 19.2385 18.0054 20.2433 31.2022
Translational Efficiency 1.5519 1.4607 1.2974 1.1349 1.8092 2.1695 1.6336 1.3949 1.6914 1.4875

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
943 619 1981 1334 1078 1424 191 775 2021 2043 2172 2109

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 817.54 892.65 1003.92 1055.80 841.11 854.20 1097.46 1057.71 830.11 865.85 1012.15 1056.50
Standard Deviation 140.96 155.15 152.54 233.62 125.23 141.72 172.51 224.08 133.32 146.99 156.65 230.16
Intensity Change Log 2 — 0.126805 0.296283 0.368975 — 0.022279 0.383802 0.330578 — 0.074746 0.341328 0.349631

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000427 0.000914 0.000482 0.000672 0.000459 0.000520 0.000759 0.001239 0.000444 0.000639 0.000507 0.000880
Bud Neck 0.002853 0.016858 0.014034 0.021630 0.004798 0.009880 0.007382 0.025469 0.003891 0.011994 0.013449 0.023041
Bud Site 0.000492 0.004192 0.000947 0.004288 0.000772 0.003469 0.000732 0.015471 0.000642 0.003688 0.000928 0.008398
Cell Periphery 0.000167 0.000340 0.000133 0.000215 0.000276 0.000362 0.000059 0.000208 0.000225 0.000355 0.000126 0.000213
Cytoplasm 0.001797 0.018572 0.002008 0.015627 0.001077 0.014520 0.003400 0.031980 0.001413 0.015747 0.002130 0.021637
Cytoplasmic Foci 0.000921 0.004098 0.000019 0.000930 0.000243 0.002389 0.000337 0.006207 0.000559 0.002907 0.000047 0.002869
Eisosomes 0.000025 0.000039 0.000037 0.000028 0.000039 0.000031 0.000046 0.000037 0.000032 0.000033 0.000038 0.000031
Endoplasmic Reticulum 0.005327 0.007650 0.003819 0.003984 0.006798 0.006144 0.004285 0.004982 0.006112 0.006600 0.003860 0.004351
Endosome 0.000174 0.000811 0.000145 0.001354 0.000321 0.001196 0.000168 0.010301 0.000252 0.001080 0.000147 0.004642
Golgi 0.000099 0.000275 0.000036 0.005156 0.000023 0.000684 0.000054 0.013538 0.000058 0.000560 0.000038 0.008236
Lipid Particles 0.000182 0.000667 0.000029 0.000105 0.000120 0.000271 0.000160 0.002177 0.000149 0.000391 0.000041 0.000866
Mitochondria 0.002183 0.001639 0.001166 0.005090 0.000921 0.002795 0.000828 0.016309 0.001509 0.002445 0.001136 0.009213
Mitotic Spindle 0.000448 0.003056 0.002013 0.016935 0.000504 0.008269 0.003085 0.023884 0.000478 0.006690 0.002107 0.019489
None 0.003482 0.004698 0.001684 0.003709 0.001317 0.005165 0.004381 0.007199 0.002327 0.005024 0.001921 0.004992
Nuclear Periphery 0.000173 0.000613 0.000494 0.001862 0.000204 0.000766 0.000261 0.001740 0.000190 0.000720 0.000474 0.001817
Nuclear Periphery Foci 0.000455 0.000800 0.000058 0.000787 0.000041 0.000631 0.000110 0.002688 0.000234 0.000682 0.000063 0.001485
Nucleolus 0.010651 0.011775 0.005743 0.004230 0.009401 0.010165 0.006772 0.005746 0.009984 0.010652 0.005834 0.004787
Nucleus 0.969042* 0.918835* 0.965946* 0.898482* 0.971174* 0.928721* 0.965495* 0.797581* 0.970179* 0.925726* 0.965906* 0.861404*
Peroxisomes 0.000480 0.001486 0.000123 0.000113 0.000420 0.000482 0.000339 0.000660 0.000448 0.000786 0.000142 0.000314
Vacuole 0.000476 0.002353 0.000852 0.007938 0.000941 0.002450 0.001272 0.023220 0.000724 0.002421 0.000889 0.013554
Vacuole Periphery 0.000145 0.000329 0.000232 0.006863 0.000151 0.001089 0.000076 0.009363 0.000148 0.000859 0.000218 0.007782

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.15 -0.58 -2.79 0.83 -5.33 -0.44 -2.50 -4.07 -5.78 -2.52 -1.64 -0.96 -4.47 -3.45 -7.32
Bud Neck -6.08 -13.57 -13.44 -2.23 -4.65 -4.77 -2.55 -10.08 -7.19 -6.30 -8.10 -12.90 -16.43 -7.94 -6.62
Bud Site -4.54 -3.73 -7.15 -0.25 -6.06 -4.28 -1.86 -6.08 -4.43 -5.68 -6.15 -3.13 -8.63 -3.98 -7.97
Cell Periphery -1.96 0.87 -0.37 1.76 -1.30 -0.80 2.87 1.42 2.27 -3.93 -1.74 2.08 0.96 2.64 -1.63
Cytoplasm -5.25 -1.07 -9.56 -1.68 -8.91 -6.46 -2.31 -10.65 -6.33 -9.40 -8.24 -1.94 -14.27 -5.78 -13.01
Cytoplasmic Foci -2.30 1.26 0.07 2.64 -4.26 -3.45 -0.33 -4.52 -1.18 -4.44 -3.62 1.44 -2.85 1.79 -6.21
Eisosomes -4.38 -5.13 -2.72 2.94 3.21 1.98 -2.31 -2.62 -5.85 0.19 -0.67 -2.64 -2.45 -1.91 0.37
Endoplasmic Reticulum -3.38 3.51 1.40 3.98 -0.97 1.10 2.89 0.40 -0.63 -2.44 -1.27 5.80 1.87 2.93 -2.81
Endosome -4.36 0.37 -4.51 -0.58 -4.63 -3.19 0.10 -4.99 -3.97 -5.02 -4.48 1.12 -6.07 -3.47 -6.36
Golgi -1.30 1.56 -2.78 -2.59 -2.85 -2.67 -3.84 -3.51 -3.08 -3.47 -2.80 0.91 -4.42 -3.81 -4.45
Lipid Particles -2.59 2.18 0.07 2.86 -7.17 -1.84 -1.04 -1.73 -1.51 -1.61 -2.93 2.09 -1.75 -0.83 -2.18
Mitochondria 0.47 0.85 -1.49 -2.69 -3.44 -2.08 -0.08 -3.72 -2.64 -3.77 -1.16 0.49 -4.03 -3.11 -4.96
Mitotic Spindle -1.74 -2.64 -7.66 -6.06 -6.61 -3.81 -1.59 -6.50 -4.30 -2.82 -4.22 -2.99 -10.02 -6.59 -8.33
None -1.34 2.56 -1.65 0.06 -4.72 -5.66 -1.99 -5.15 -1.42 -1.37 -4.62 0.79 -5.56 -0.73 -5.59
Nuclear Periphery -6.64 -4.17 -5.89 -4.47 -4.62 -3.54 -2.36 -10.82 -6.52 -8.96 -4.84 -4.15 -9.03 -6.11 -7.07
Nuclear Periphery Foci -0.94 1.21 -0.62 0.47 -2.93 -2.86 -1.80 -3.21 -1.83 -2.80 -1.68 1.07 -2.57 -1.21 -4.23
Nucleolus -1.08 3.49 4.06 5.07 0.96 -0.36 1.90 2.26 2.88 0.29 -0.69 4.10 4.65 5.71 0.63
Nucleus 7.19 2.00 13.92 4.70 13.47 8.33 2.43 17.31 12.60 12.91 10.77 3.43 21.99 12.53 20.20
Peroxisomes -1.39 2.33 2.37 1.93 0.38 -0.35 0.33 -0.16 0.28 -0.95 -1.32 2.34 1.56 2.34 -2.31
Vacuole -4.49 -2.31 -9.28 -7.84 -8.78 -3.40 -1.83 -10.71 -10.36 -9.48 -5.25 -2.38 -13.74 -12.79 -13.27
Vacuole Periphery -2.53 -1.13 -3.31 -3.15 -3.23 -2.24 1.94 -3.68 -2.67 -3.73 -2.44 -1.17 -4.64 -3.65 -4.53
Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rfc4

Rfc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available