Standard name
Description Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.07 0.05 0 0 0 0.07 0 0.08 0.19 0.22 0.09 0.11 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.14 0.25 0.2 0.4 0.29 0.49 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.94 0.94 0.94 0.88 0.84 0.85 0.82 0.83 0.73 0.81 0.9 0.77 0.67 0.8 0.82 0.84 0.94 0.91 0.93 0.73 0.67 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.06 0 0.1 0.08 0.09 0.11 0 0 0.05 0 0 0 0 0 0 0.07 0.06 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.05 0 0 0 0 0.11 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 1 0 1 6 6 2 1 4 2 1 0 0 1 0 1 3 1 0 2 5 2
Bud Neck 0 0 0 0 1 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 2 10 2 8 9 15 10 10 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 5 7 13 24 18 13 14 13 15 9 12 38 45 21 22 25 0 1 0 4 1 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 10 0 4 0 0 0 0 0 1
Endosome 1 0 2 0 0 0 0 0 0 0 0 0 0 0 3 4 1 1 0 2 1 0 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 0 1 0 0 1 0
Mitochondria 20 4 3 8 2 47 92 75 138 105 111 55 2 0 0 7 4 9 2 1 2 2 3 5
Nucleus 334 230 163 189 266 294 307 320 280 296 165 174 131 154 134 194 159 194 327 229 158 121 150 138
Nuclear Periphery 8 1 0 1 0 4 2 1 1 3 4 0 1 1 3 2 3 2 0 0 0 1 1 8
Nucleolus 11 1 4 0 3 8 21 13 34 28 20 24 0 3 10 5 3 2 2 3 1 10 12 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 1 0 3 0 3 1 1 1 1 1 3 0 0 2 1 0 0 2 1
Vac/Vac Membrane 7 6 1 2 1 2 3 3 6 6 1 4 1 3 16 19 9 8 10 8 4 18 37 37
Unique Cell Count 350 245 173 202 282 333 365 378 341 356 225 214 146 199 201 242 195 231 350 253 171 167 225 215
Labelled Cell Count 385 247 180 209 290 391 451 444 485 470 331 279 149 201 213 267 204 248 350 253 171 167 225 215


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 9.2 7.2 7.1 7.2 5.5 5.1 5.5 5.0 5.5 4.8 5.1 7.9 8.4 9.0 8.6 9.4 8.9 8.2 8.4 8.6
Std Deviation (1e-4) 1.5 2.0 1.6 1.8 2.0 1.8 1.5 1.4 1.7 1.9 1.9 1.5 2.0 3.2 4.7 2.4 2.4 2.6 2.5 2.9 2.7
Intensity Change (Log2) -0.03 -0.01 -0.38 -0.49 -0.39 -0.53 -0.4 -0.6 -0.52 0.13 0.22 0.32 0.25 0.37 0.3 0.17 0.21 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.9 2.0 1.1 0.3 0.7 0.5 1.7 0.7 2.1 4.9 5.5 2.4 3.1 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 0 4.4 6.7 5.7 9.3 7.4 0 6.6 0 0 0 0 0 1.3
Nucleus -0.3 0 -2.1 -3.3 -3.2 -3.8 -3.5 -5.4 -3.8 -1.5 -4.6 -6.6 -4.1 -3.7 -3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1.8 0.7 3.1 2.5 0 3.4 0 0 1.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.4 3.4 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available