Standard name
Description Member of the protein disulfide isomerase (PDI) family; exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.32 0.45 0.33 0.62 0.61 0.72 0.65 0.65 0.77 0.77 0.8 0.84 0.72 0.86 0.91 0.32 0.4 0.27 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.06 0 0 0 0.07 0.08 0.08 0.09 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.09 0.08 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0.21 0.26 0.26 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.73 0.54 0.75 0.61 0.58 0.47 0.46 0.36 0.32 0.32 0.23 0.24 0.29 0.16 0.11 0.64 0.55 0.65 0.9 0.86 0.92 0.86 0.89 0.89
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 1 2 6 6 11 3 9 2 0 0 0 0 0 1 3 1 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 1 0 0 0 0 2 0 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 49 114 77 124 193 313 278 367 361 420 357 523 144 206 268 59 60 42 4 13 4 5 9 7
Endoplasmic Reticulum 1 2 0 0 1 0 0 2 1 1 0 0 7 4 3 8 1 13 0 2 5 7 3 7
Endosome 0 0 16 2 6 4 1 1 1 3 2 4 3 2 1 5 1 3 3 5 1 3 7 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 4
Mitochondria 6 31 13 1 1 13 30 46 39 48 41 32 0 0 0 5 1 2 1 10 3 3 6 5
Nucleus 13 22 18 7 7 9 27 38 14 11 6 7 5 3 3 39 39 41 1 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 3 1 9 0 2 1 3 0 0 1 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 1 0 0 0 1 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1
Vac/Vac Membrane 111 139 177 122 182 205 196 200 151 175 101 147 58 38 32 117 83 101 148 253 229 161 297 371
Unique Cell Count 153 256 236 201 315 433 430 563 471 549 446 623 200 239 293 183 151 155 164 293 249 188 333 418
Labelled Cell Count 181 308 301 257 391 548 536 670 575 671 512 727 219 254 309 237 185 203 164 293 249 188 333 418


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.7 5.7 5.8 6.2 5.2 5.0 5.2 4.9 4.9 4.8 4.8 5.9 5.5 5.3 7.0 6.8 7.1 5.7 5.7 6.1
Std Deviation (1e-4) 0.9 0.8 1.4 1.4 1.6 1.4 1.4 1.2 1.0 1.2 0.9 1.1 0.9 0.8 0.9 1.6 1.4 1.7 1.0 1.2 1.5
Intensity Change (Log2) – – – 0.03 0.12 -0.14 -0.18 -0.14 -0.23 -0.21 -0.26 -0.26 0.04 -0.06 -0.11 0.3 0.24 0.31 -0.0 0.01 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -2.5 -4.1 -2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3783 1.4992 1.1001 0.5779 0.9324 1.3536 1.6325 2.6156 2.4514 2.237 2.5395 2.766 0.6142 1.8015 1.253 1.4222 0.8254 1.4423
Actin 0.0098 0 0.0032 0 0.0001 0.0001 0.002 0.0001 0.0002 0 0.0001 0 0.0064 0 0.0012 0.0001 0.0001 0
Bud 0.0003 0.0003 0.0006 0.0006 0.0004 0.0004 0.0004 0.0004 0.0005 0.0007 0.0009 0.0005 0.0005 0.0004 0.0008 0.0005 0.0005 0.0007
Bud Neck 0.0011 0.0011 0.001 0.0015 0.0016 0.0018 0.0015 0.0017 0.001 0.0012 0.024 0.0028 0.0012 0.0013 0.0028 0.0013 0.0013 0.0036
Bud Periphery 0.0009 0.0006 0.0014 0.0012 0.0012 0.0012 0.0009 0.001 0.0011 0.001 0.0011 0.0016 0.0011 0.0016 0.0016 0.0018 0.0006 0.0024
Bud Site 0.0018 0.0017 0.0017 0.0028 0.0011 0.0009 0.0018 0.0021 0.0051 0.004 0.0078 0.0016 0.0051 0.0015 0.0045 0.0012 0.0023 0.0016
Cell Periphery 0.0016 0.0023 0.0013 0.0016 0.0013 0.0026 0.0015 0.0024 0.0018 0.0008 0.0019 0.0028 0.0026 0.0052 0.0027 0.0027 0.0007 0.0038
Cytoplasm 0.051 0.0489 0.0473 0.0205 0.0282 0.0349 0.0489 0.048 0.055 0.0589 0.0228 0.0395 0.0489 0.0274 0.0538 0.0159 0.0258 0.0277
Cytoplasmic Foci 0.0221 0.0176 0.0203 0.0096 0.0211 0.014 0.0266 0.0214 0.0297 0.0181 0.0123 0.0189 0.0157 0.0117 0.0305 0.0116 0.0158 0.0098
Eisosomes 0.0004 0 0.0007 0 0 0 0.0001 0.0001 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0215 0.009 0.018 0.0031 0.0112 0.0087 0.0241 0.0145 0.0201 0.027 0.0081 0.0185 0.0261 0.0285 0.0168 0.0053 0.0059 0.0109
Endosome 0.3139 0.2205 0.2653 0.2095 0.1678 0.1662 0.3038 0.1928 0.3147 0.308 0.2667 0.2031 0.2796 0.1802 0.262 0.2084 0.3268 0.1859
Golgi 0.0057 0.0026 0.0054 0.0032 0.002 0.0033 0.0063 0.0029 0.0074 0.0041 0.0049 0.0031 0.0064 0.0049 0.0142 0.0094 0.0118 0.0053
Lipid Particles 0.0207 0.009 0.0145 0.0047 0.0073 0.0093 0.016 0.0046 0.0125 0.0046 0.0123 0.01 0.0176 0.0134 0.0171 0.0087 0.0069 0.0149
Mitochondria 0.0061 0.0047 0.0058 0.0006 0.0044 0.003 0.0115 0.0043 0.0096 0.004 0.0023 0.0034 0.0069 0.0034 0.0194 0.0046 0.0019 0.0024
None 0.0309 0.0488 0.0393 0.0019 0.0494 0.0029 0.0414 0.058 0.0228 0.0035 0.0088 0.0109 0.0417 0.0208 0.0097 0.0289 0.0004 0.0072
Nuclear Periphery 0.0234 0.0073 0.0194 0.0033 0.009 0.0099 0.021 0.0108 0.0149 0.0075 0.0045 0.0172 0.0266 0.0221 0.0172 0.0041 0.0038 0.0067
Nucleolus 0.0029 0.0024 0.0023 0.0042 0.0024 0.0014 0.0028 0.004 0.0023 0.0016 0.0022 0.0028 0.002 0.0016 0.0016 0.0023 0.0011 0.003
Nucleus 0.0077 0.0059 0.0088 0.0061 0.0086 0.0079 0.0105 0.0225 0.0086 0.0124 0.0046 0.0124 0.0055 0.0051 0.0055 0.0037 0.0047 0.0171
Peroxisomes 0.0067 0.0003 0.0016 0.0003 0.0009 0.0007 0.0019 0.0004 0.0011 0.0004 0.0006 0.0006 0.0009 0.0003 0.0035 0.0007 0.0004 0.0004
Punctate Nuclear 0.0041 0.0007 0.0009 0.0003 0.0006 0.0004 0.0018 0.0018 0.0014 0.0005 0.0005 0.0027 0.001 0.0002 0.0016 0.0003 0.0002 0.0003
Vacuole 0.4154 0.5684 0.4771 0.6745 0.6164 0.6662 0.4271 0.5532 0.4304 0.4983 0.5753 0.5901 0.4518 0.6057 0.471 0.6044 0.5473 0.6385
Vacuole Periphery 0.0518 0.0477 0.064 0.0506 0.0649 0.0643 0.0481 0.0528 0.0596 0.0431 0.0384 0.0573 0.0524 0.0646 0.0623 0.0842 0.0415 0.0576

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.6444 35.4812 29.839 27.1006 28.3035 29.0905 36.1739 26.6345 34.9681 32.4261
Translational Efficiency 0.9949 1.2018 1.0719 1.2404 1.0629 1.2064 1.0644 1.2414 0.9687 0.8782

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘