Standard name
Human Ortholog
Description GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.17 0.14 0 0 0 0 0 0
Cytoplasm 0.98 0.95 0.97 0.98 0.95 0.92 0.8 0.72 0.7 0.72 0.6 0.99 0.99 0.99 0.92 0.88 0.94 0.96 0.97 0.87 0.93 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.08 0.26 0.35 0.55 0.46 0.54 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 2 0 2 0 0 0 1 1 0 2 0 0 0 2 0
Bud 2 2 2 3 1 2 10 10 7 15 9 0 2 0 0 0 0 0 0 0 0 3 2
Bud Neck 1 0 0 0 0 0 3 0 4 1 4 0 0 0 0 0 0 1 0 0 0 1 2
Bud Site 0 0 0 0 1 0 1 1 2 0 3 0 0 0 0 0 0
Cell Periphery 7 1 2 9 4 5 7 6 8 9 11 8 19 41 16 32 29 0 0 0 0 0 0
Cytoplasm 346 52 117 283 392 381 532 358 331 338 346 390 457 516 169 161 199 343 71 117 107 315 324
Endoplasmic Reticulum 1 0 1 0 0 3 2 2 3 1 7 1 0 3 8 11 12 0 0 1 1 5 3
Endosome 0 0 0 1 0 0 1 0 0 0 0 0 0 1 4 5 2 3 0 0 0 0 6
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 3 2 0 0 0 1 1
Mitochondria 7 1 5 28 31 107 234 272 217 253 348 2 2 0 5 7 4 1 0 3 1 5 8
Nucleus 0 0 0 0 0 1 3 1 0 1 1 2 2 0 1 0 1 0 0 0 0 1 1
Nuclear Periphery 1 1 0 0 0 0 3 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 1 2 2 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 2 1 0 6 0 0 0 1 0 1 0 0 1 0 0 2
Vac/Vac Membrane 0 1 0 0 1 2 0 2 3 0 0 0 0 2 6 15 6 0 0 5 2 8 6
Unique Cell Count 352 55 121 290 413 414 663 496 470 471 578 392 461 520 183 183 211 358 73 135 116 352 367
Labelled Cell Count 366 58 127 325 435 503 799 656 579 619 739 403 483 563 211 234 257 358 73 135 116 352 367


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.9 3.8 4.3 4.9 3.8 3.8 3.1 3.5 3.1 3.2 5.7 5.9 5.8 7.3 7.3 7.0 5.6 6.3 6.4
Std Deviation (1e-4) 0.6 0.8 0.9 1.1 1.8 0.9 1.0 0.5 0.7 0.7 0.6 0.7 0.9 1.2 1.2 1.4 1.3 0.6 1.4 1.1
Intensity Change (Log2) 0.17 0.34 -0.03 -0.01 -0.33 -0.13 -0.29 -0.27 0.57 0.61 0.6 0.91 0.92 0.85 0.53 0.7 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 2.5 2.6 4.3 3.6
Cytoplasm 0.5 -0.8 -1.8 -4.4 -5.7 -6.0 -5.8 -7.8 2.5 2.1 2.3 -1.6 -2.7 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0829 2.8243 2.3646 2.1994 2.0209 2.6099 0.6428 1.4835 1.2052 0.8181 1.0824 1.2681 1.7987 3.1056 2.4014 2.9745 2.4788 2.5262
Actin 0.0214 0.0015 0.0061 0.0012 0.0088 0.0076 0.0038 0.0012 0.0014 0.0121 0.0054 0.0014 0.0156 0.0003 0.0201 0.0162 0.0013 0.0013
Bud 0.0005 0.0026 0.0018 0.0043 0.0118 0.0024 0.0004 0.0001 0.0002 0.0008 0.0007 0.0018 0.0007 0.0002 0.0022 0.0019 0.0004 0.0001
Bud Neck 0.0029 0.0006 0.0029 0.0011 0.0041 0.0213 0.0008 0.0002 0.0006 0.0008 0.0011 0.0053 0.0067 0.0001 0.0015 0.0118 0.0002 0.0004
Bud Periphery 0.0007 0.0015 0.0049 0.0085 0.0226 0.0037 0.0004 0.0001 0.0003 0.001 0.0012 0.0027 0.0011 0.0002 0.0041 0.003 0.0007 0.0001
Bud Site 0.0074 0.0253 0.0074 0.0015 0.0105 0.0036 0.0055 0.0015 0.0013 0.0068 0.0011 0.0046 0.0107 0.0021 0.0046 0.0261 0.0003 0.0001
Cell Periphery 0.0026 0.0015 0.002 0.0009 0.0014 0.0006 0.0008 0.0006 0.0006 0.0003 0.0002 0.0005 0.0016 0.0005 0.0008 0.001 0.0002 0.0002
Cytoplasm 0.7024 0.7476 0.6875 0.6399 0.7465 0.7403 0.5967 0.8242 0.7564 0.6078 0.6605 0.877 0.5227 0.7863 0.6625 0.5782 0.8228 0.7781
Cytoplasmic Foci 0.02 0.0088 0.0244 0.0309 0.0092 0.007 0.0441 0.0074 0.0063 0.0489 0.0434 0.0078 0.0129 0.0026 0.0053 0.0122 0.0063 0.0028
Eisosomes 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0 0 0.0004 0.0001 0.0004 0.0002 0 0
Endoplasmic Reticulum 0.0035 0.0027 0.0038 0.0019 0.0036 0.0031 0.0078 0.0028 0.0035 0.0067 0.0046 0.0018 0.0047 0.0016 0.0023 0.0076 0.0029 0.0011
Endosome 0.0094 0.0019 0.0057 0.011 0.008 0.0066 0.0494 0.0014 0.0094 0.0728 0.0605 0.0051 0.0202 0.0008 0.0052 0.0306 0.0166 0.001
Golgi 0.0025 0.0004 0.0013 0.0017 0.0006 0.0023 0.0095 0.0004 0.002 0.0203 0.0325 0.001 0.005 0.0001 0.0065 0.0095 0.0062 0.0004
Lipid Particles 0.0045 0.0003 0.0016 0.0116 0.0003 0.0027 0.0153 0.0005 0.0038 0.0104 0.0125 0.0005 0.0088 0.0001 0.0009 0.0021 0.0011 0.0001
Mitochondria 0.0007 0.0002 0.0004 0.0011 0.0006 0.0043 0.0069 0.0001 0.004 0.0173 0.0289 0.0007 0.0017 0.0001 0.0029 0.0093 0.035 0.0001
None 0.2052 0.1996 0.2415 0.2595 0.159 0.1856 0.2092 0.1569 0.2039 0.1675 0.1258 0.072 0.3611 0.2036 0.2763 0.2692 0.1012 0.2134
Nuclear Periphery 0.0016 0.0004 0.0007 0.0004 0.0014 0.0028 0.0085 0.0002 0.0004 0.003 0.0013 0.0005 0.0027 0.0001 0.0004 0.0069 0.0008 0.0001
Nucleolus 0.0004 0.0002 0.0003 0.0016 0.0009 0.0003 0.0009 0.0001 0.0002 0.0003 0.0002 0.0024 0.0046 0 0.0001 0.0005 0.0001 0
Nucleus 0.0014 0.0011 0.0012 0.0013 0.0035 0.0018 0.0173 0.0005 0.0005 0.0014 0.0007 0.0055 0.0049 0.0003 0.0006 0.0032 0.0006 0.0002
Peroxisomes 0.0045 0.0002 0.0014 0.0129 0.0003 0.0008 0.0051 0.0002 0.001 0.0093 0.0075 0.0003 0.0041 0 0.001 0.0013 0.0004 0
Punctate Nuclear 0.0055 0.0014 0.0024 0.0018 0.0013 0.0008 0.0078 0.0003 0.0002 0.0044 0.0004 0.0007 0.0026 0.0001 0.0002 0.0016 0.0003 0.0001
Vacuole 0.0025 0.0019 0.0025 0.0062 0.0049 0.002 0.0078 0.0011 0.0035 0.0061 0.0071 0.0078 0.0065 0.0009 0.0018 0.0054 0.0018 0.0005
Vacuole Periphery 0.0002 0.0001 0.0002 0.0005 0.0006 0.0004 0.0017 0.0001 0.0004 0.0018 0.0042 0.0008 0.0007 0.0001 0.0002 0.0022 0.0008 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.0181 29.6767 24.0809 35.4415 24.3854 26.7344 36.1314 38.2458 31.315 36.2641
Translational Efficiency 0.1723 0.1551 0.1876 0.1129 0.1257 0.1727 0.1563 0.0958 0.135 0.1099

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
36 1417 448 159 1180 1374 1317 51 1216 2791 1765 210

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 850.90 789.39 965.91 974.34 691.96 783.33 776.02 833.15 696.67 786.41 824.22 940.05
Standard Deviation 120.06 114.09 108.11 101.26 91.12 99.40 92.21 79.88 95.96 107.15 127.05 113.93
Intensity Change Log 2 -0.108251 0.182899 0.195436 0.178932 0.165405 0.267888 0.027655 0.175079 0.228381

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000646 0.000319 0.000261 0.000291 0.000367 0.000324 0.000296 0.000213 0.000375 0.000322 0.000288 0.000272
Bud Neck 0.002583 0.013866 0.002243 0.003543 0.022208 0.032034 0.006150 0.003414 0.021627 0.022810 0.005158 0.003511
Bud Site 0.004629 0.023830 0.017147 0.052381 0.010946 0.024164 0.034428 0.052057 0.010759 0.023994 0.030042 0.052302
Cell Periphery 0.000689 0.000151 0.000240 0.000130 0.000296 0.000174 0.000150 0.000864 0.000308 0.000162 0.000173 0.000308
Cytoplasm 0.478631* 0.589360* 0.598822* 0.464220* 0.498218* 0.507579* 0.686619* 0.503101* 0.497638* 0.549100* 0.664334* 0.473662*
Cytoplasmic Foci 0.175570 0.203605* 0.131543 0.080465 0.283700* 0.244915* 0.089533 0.102847 0.280499* 0.223942* 0.100196 0.085900
Eisosomes 0.000390 0.000125 0.000075 0.000054 0.000608 0.000224 0.000064 0.000131 0.000602 0.000174 0.000066 0.000073
Endoplasmic Reticulum 0.008129 0.000663 0.001058 0.000517 0.001483 0.000700 0.000309 0.000613 0.001680 0.000681 0.000499 0.000540
Endosome 0.007146 0.025060 0.013878 0.009414 0.029983 0.037498 0.006907 0.016896 0.029307 0.031183 0.008677 0.011231
Golgi 0.000542 0.008043 0.000335 0.000176 0.009431 0.008055 0.001062 0.016437 0.009168 0.008049 0.000878 0.004125
Lipid Particles 0.009986 0.003077 0.002108 0.001084 0.011407 0.004155 0.000868 0.001935 0.011365 0.003608 0.001183 0.001291
Mitochondria 0.001264 0.003672 0.000062 0.000535 0.002654 0.004188 0.000811 0.004295 0.002613 0.003926 0.000621 0.001448
Mitotic Spindle 0.028576 0.003375 0.001004 0.007776 0.000304 0.001983 0.004864 0.000164 0.001141 0.002690 0.003884 0.005928
None 0.000378 0.001609 0.001105 0.000679 0.000825 0.000978 0.000564 0.000818 0.000812 0.001298 0.000702 0.000713
Nuclear Periphery 0.008562 0.000197 0.000241 0.000284 0.000234 0.000097 0.000072 0.000391 0.000480 0.000148 0.000115 0.000310
Nuclear Periphery Foci 0.013098 0.000836 0.005050 0.002077 0.000486 0.000239 0.000683 0.001467 0.000859 0.000542 0.001792 0.001929
Nucleolus 0.001068 0.000387 0.000192 0.000152 0.000377 0.000295 0.000104 0.000327 0.000397 0.000342 0.000126 0.000194
Nucleus 0.105114 0.020442 0.032944 0.013171 0.034887 0.022686 0.023184 0.068652 0.036966 0.021547 0.025661 0.026645
Peroxisomes 0.001353 0.004312 0.001531 0.000953 0.004024 0.005065 0.001217 0.000468 0.003945 0.004683 0.001297 0.000835
Vacuole 0.151072 0.095412 0.189892 0.361356* 0.085668 0.102292 0.141268 0.223750* 0.087605 0.098799 0.153610 0.327937*
Vacuole Periphery 0.000574 0.001658 0.000268 0.000743 0.001892 0.002353 0.000848 0.001160 0.001853 0.002000 0.000701 0.000844

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.10 1.32 1.56 3.24 1.12 0.48 1.08 1.47 1.01 0.74 0.78 1.39 2.69 2.63 1.70
Bud Neck -9.83 0.22 0.51 11.00 0.53 -3.82 9.71 10.77 12.88 2.29 -0.61 10.59 12.64 17.01 3.70
Bud Site -9.01 -3.41 -2.21 0.62 -0.51 -7.87 -6.81 -1.11 -0.53 -0.23 -10.00 -6.88 -1.70 0.06 0.46
Cell Periphery 4.13 3.52 4.77 5.90 7.17 8.15 9.28 -0.89 -1.20 -1.29 9.92 9.06 0.95 -0.52 -0.55
Cytoplasm -2.62 -2.29 1.44 8.79 7.54 -0.93 -17.61 0.82 1.07 5.54 -6.09 -16.15 4.83 7.43 11.93
Cytoplasmic Foci -1.20 1.57 3.72 18.75 7.71 5.28 32.48 14.52 11.71 -0.14 8.93 31.45 28.38 24.25 4.82
Eisosomes 3.76 4.54 4.95 9.99 4.66 8.01 12.36 9.91 3.49 -3.72 9.71 12.49 12.31 9.29 -0.58
Endoplasmic Reticulum 1.31 1.22 1.35 0.96 5.45 3.29 5.77 1.32 -0.66 -1.80 3.56 4.63 4.10 0.67 -0.66
Endosome -7.75 -2.82 1.39 10.26 5.20 -3.04 13.86 3.54 5.20 -2.17 -0.91 12.87 10.74 13.36 0.59
Golgi -8.65 1.35 2.92 9.16 3.76 1.34 12.27 -0.14 -0.27 -0.93 1.36 12.79 2.37 1.95 -0.75
Lipid Particles 2.24 2.62 3.00 6.07 4.81 7.45 11.25 7.16 2.05 -1.75 8.31 11.20 10.84 7.51 -0.57
Mitochondria -2.08 1.23 1.04 3.67 -0.82 -1.33 2.78 -0.09 0.46 -0.82 -1.40 3.09 1.90 3.28 -0.46
Mitotic Spindle 0.87 0.97 0.78 -0.77 -1.76 -2.02 -3.26 0.40 2.11 3.32 -1.78 -2.21 -1.55 -0.72 -0.19
None -3.82 -3.20 -2.14 2.23 0.94 -0.36 2.21 -0.33 0.06 -1.17 -1.75 1.16 0.01 1.60 -0.81
Nuclear Periphery 1.03 1.02 1.00 -2.39 -1.95 3.31 3.50 -2.37 -3.44 -3.48 1.41 1.47 0.08 -4.84 -5.82
Nuclear Periphery Foci 1.23 0.76 1.12 -3.31 6.65 3.46 -2.50 -1.56 -1.75 -1.41 0.94 -3.25 -2.65 -3.93 -0.62
Nucleolus 0.79 1.13 1.24 5.21 2.33 1.73 6.60 -0.11 -0.65 -1.91 0.90 6.07 3.49 3.32 -1.23
Nucleus 3.37 2.74 3.55 2.33 6.32 6.25 4.51 -3.83 -4.83 -4.57 8.20 4.17 1.14 -2.75 -0.97
Peroxisomes -6.98 -0.14 2.40 10.99 1.30 -1.83 6.24 10.36 8.73 2.54 -1.77 6.41 10.86 13.36 2.80
Vacuole 2.39 -0.63 -6.58 -16.77 -10.83 -3.70 -12.94 -6.06 -5.54 -3.65 -2.72 -15.66 -17.06 -16.66 -12.78
Vacuole Periphery -3.59 0.98 0.65 4.94 -0.19 -2.31 3.48 2.08 3.01 -0.17 -0.76 4.69 5.94 6.99 0.66