Standard name | |
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Human Ortholog | |
Description | Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 2 | 0 | 0 | 1 |
Bud | 0 | 2 | 0 | 0 | 0 | 0 | 2 | – | 5 | 2 | 4 | 5 | 0 | 0 | 0 | – | – | – | 3 | 3 | 6 | 2 | 3 | 8 |
Bud Neck | 0 | 1 | 6 | 3 | 2 | 1 | 5 | – | 2 | 7 | 2 | 4 | 0 | 8 | 1 | – | – | – | 0 | 2 | 2 | 1 | 2 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 3 | 4 | – | 17 | 2 | 12 | 41 | 0 | 2 | 3 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 3 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 11 | 2 | 9 | 8 | 9 | 2 | 7 | – | 7 | 6 | 4 | 10 | 15 | 36 | 49 | – | – | – | 0 | 7 | 0 | 16 | 7 | 21 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 3 | 3 | 2 | 12 | 23 | 56 | – | 9 | 27 | 34 | 38 | 2 | 3 | 5 | – | – | – | 0 | 7 | 9 | 2 | 7 | 9 |
Golgi | 0 | 0 | 0 | 0 | 1 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 1 | 1 | 1 | 1 |
Mitochondria | 93 | 70 | 63 | 52 | 49 | 56 | 47 | – | 123 | 53 | 152 | 108 | 52 | 28 | 36 | – | – | – | 5 | 14 | 20 | 5 | 14 | 13 |
Nucleus | 97 | 80 | 52 | 8 | 28 | 33 | 51 | – | 38 | 36 | 62 | 64 | 4 | 16 | 12 | – | – | – | 60 | 72 | 34 | 17 | 9 | 16 |
Nuclear Periphery | 1 | 2 | 2 | 0 | 3 | 5 | 4 | – | 6 | 4 | 5 | 4 | 0 | 1 | 0 | – | – | – | 15 | 7 | 11 | 2 | 4 | 7 |
Nucleolus | 315 | 362 | 481 | 383 | 363 | 513 | 742 | – | 537 | 580 | 791 | 752 | 197 | 305 | 304 | – | – | – | 332 | 335 | 450 | 169 | 168 | 158 |
Peroxisomes | 0 | 1 | 7 | 16 | 9 | 46 | 43 | – | 16 | 31 | 20 | 21 | 7 | 7 | 6 | – | – | – | 0 | 1 | 0 | 0 | 2 | 0 |
SpindlePole | 1 | 1 | 24 | 35 | 53 | 110 | 211 | – | 98 | 146 | 151 | 213 | 16 | 42 | 29 | – | – | – | 3 | 12 | 40 | 9 | 16 | 9 |
Vac/Vac Membrane | 22 | 12 | 14 | 3 | 9 | 6 | 15 | – | 0 | 7 | 9 | 9 | 1 | 1 | 2 | – | – | – | 19 | 22 | 11 | 7 | 11 | 15 |
Unique Cell Count | 417 | 451 | 559 | 411 | 433 | 599 | 871 | 627 | 673 | 934 | 909 | 216 | 358 | 379 | 445 | 494 | 601 | 241 | 254 | 272 | ||||
Labelled Cell Count | 542 | 536 | 661 | 510 | 538 | 800 | 1187 | 858 | 901 | 1246 | 1269 | 295 | 449 | 450 | 445 | 494 | 601 | 241 | 254 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 35.0 | 23.5 | 24.1 | 23.1 | 14.9 | 15.7 | 14.5 | – | 12.7 | 13.7 | 12.7 | 12.4 | 25.8 | 20.2 | 21.9 | – | – | – | 29.0 | 28.3 | 27.5 |
Std Deviation (1e-4) | 14.3 | 9.1 | 8.4 | 8.7 | 5.2 | 5.5 | 4.7 | – | 4.8 | 4.5 | 4.1 | 4.7 | 11.3 | 10.3 | 10.7 | – | – | – | 12.2 | 10.8 | 11.0 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.69 | -0.62 | -0.73 | – | -0.93 | -0.81 | -0.92 | -0.96 | 0.1 | -0.26 | -0.14 | – | – | – | 0.27 | 0.23 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | -0.1 | 0 | 0 | 0 | 1.4 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | – | 3.9 | 0 | 0 | 5.1 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0.4 | 0.5 | -2.2 | -1.4 | – | -0.7 | -1.1 | 0 | -0.8 | 3.8 | 5.8 | 7.1 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 2.9 | 3.8 | 5.5 | – | 1.5 | 3.9 | 3.7 | 4.1 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0.7 | 0 | -1.1 | -4.1 | – | 3.9 | -2.0 | 2.7 | 0.4 | 4.5 | -1.7 | -0.9 | – | – | – |
Nucleus | -4.7 | -1.6 | -2.5 | -2.5 | – | -2.1 | -2.7 | -1.9 | -1.6 | -3.6 | -2.7 | -3.7 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 3.5 | -1.0 | -0.2 | -0.4 | – | -0.2 | 0.1 | -0.7 | -1.7 | 1.9 | -0.4 | -2.4 | – | – | – |
Peroxisomes | 2.7 | 1.0 | 5.2 | 3.7 | – | 1.6 | 3.4 | 1.2 | 1.4 | 0 | 0.8 | 0.4 | – | – | – |
SpindlePole | 2.7 | 4.6 | 7.5 | 9.9 | – | 6.4 | 8.8 | 6.9 | 9.7 | 1.8 | 4.3 | 2.2 | – | – | – |
Vacuole | -2.1 | -0.4 | -2.0 | -1.0 | – | -4.0 | -2.0 | -2.3 | -2.3 | 0 | -2.6 | -2.3 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 25.7178 | 32.1669 | 29.3956 | 27.5117 | 25.7885 | 29.7196 | 23.6403 | 33.5631 | 30.0399 | 26.725 | 29.7018 | 29.8867 | 20.53 | 26.0795 | 22.6963 | 24.4341 | 22.9869 | 22.794 |
Actin | 0.0041 | 0 | 0.0014 | 0.0032 | 0.0024 | 0 | 0.0434 | 0 | 0 | 0.0001 | 0.0003 | 0.0054 | 0.0109 | 0 | 0.0195 | 0 | 0.0614 | 0.0002 |
Bud | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 |
Bud Neck | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0 | 0 | 0.0001 | 0.0002 | 0.0009 | 0.0004 | 0 | 0.0001 | 0 | 0.0017 | 0.0001 |
Bud Periphery | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0006 | 0.0001 |
Bud Site | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0037 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0043 | 0.0001 |
Cell Periphery | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0006 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0009 | 0.0005 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0003 |
Cytoplasm | 0.0041 | 0 | 0.0002 | 0.0002 | 0.0008 | 0.0003 | 0.0006 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0082 | 0 | 0.0021 | 0 | 0.0003 | 0.0055 |
Cytoplasmic Foci | 0.0148 | 0.0008 | 0.0086 | 0.0015 | 0.0093 | 0.0037 | 0.0194 | 0.0005 | 0.0087 | 0.0136 | 0.024 | 0.0031 | 0.0149 | 0.0001 | 0.0112 | 0.0003 | 0.0043 | 0.0004 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0017 | 0 | 0.0001 | 0.0022 | 0.0006 | 0 | 0.0008 | 0 | 0 | 0 | 0.0001 | 0 | 0.0015 | 0 | 0.0013 | 0 | 0.0001 | 0.0006 |
Endosome | 0.006 | 0 | 0.0023 | 0.0086 | 0.0054 | 0.0002 | 0.0055 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0054 | 0 | 0.0043 | 0 | 0.0019 | 0.0006 |
Golgi | 0.0017 | 0 | 0.0022 | 0.0057 | 0.0066 | 0.0001 | 0.0093 | 0 | 0 | 0 | 0.0003 | 0.0005 | 0.0024 | 0 | 0.0009 | 0 | 0.0057 | 0.0001 |
Lipid Particles | 0.0103 | 0.0035 | 0.0046 | 0.0059 | 0.0159 | 0.0088 | 0.024 | 0.0026 | 0.0016 | 0.0093 | 0.0325 | 0.0182 | 0.0196 | 0.0005 | 0.0078 | 0.0034 | 0.0036 | 0.0066 |
Mitochondria | 0.0266 | 0.0004 | 0.0081 | 0.0148 | 0.0202 | 0.0452 | 0.0079 | 0.0002 | 0.0032 | 0.0095 | 0.0297 | 0.0103 | 0.0093 | 0.0014 | 0.0022 | 0.0066 | 0.0036 | 0.0246 |
None | 0.0031 | 0 | 0.0004 | 0.0002 | 0.001 | 0.0008 | 0.0013 | 0 | 0.0003 | 0.0008 | 0.0037 | 0.0001 | 0.0012 | 0 | 0.001 | 0 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0291 | 0.0003 | 0.003 | 0.0051 | 0.0325 | 0.0061 | 0.0032 | 0 | 0.0002 | 0.0008 | 0.0081 | 0.0122 | 0.0072 | 0.0002 | 0.0008 | 0.0001 | 0.0005 | 0.0151 |
Nucleolus | 0.7935 | 0.8932 | 0.8961 | 0.8731 | 0.7516 | 0.8459 | 0.7853 | 0.939 | 0.9457 | 0.9012 | 0.7858 | 0.8668 | 0.8074 | 0.9043 | 0.8987 | 0.9374 | 0.7734 | 0.8595 |
Nucleus | 0.0699 | 0.1002 | 0.0627 | 0.0743 | 0.1367 | 0.068 | 0.0555 | 0.0572 | 0.0368 | 0.0593 | 0.0935 | 0.0656 | 0.0875 | 0.0884 | 0.043 | 0.0495 | 0.1298 | 0.0785 |
Peroxisomes | 0.0018 | 0 | 0.0014 | 0.0005 | 0.0021 | 0.0003 | 0.0168 | 0 | 0.0003 | 0.0006 | 0.0036 | 0.0004 | 0.0016 | 0 | 0.0022 | 0.0001 | 0.005 | 0.0002 |
Punctate Nuclear | 0.0223 | 0.0003 | 0.0025 | 0.0004 | 0.0099 | 0.0014 | 0.0037 | 0.0001 | 0.0007 | 0.0009 | 0.002 | 0.0005 | 0.0084 | 0.0005 | 0.001 | 0.0002 | 0.0008 | 0.0013 |
Vacuole | 0.0027 | 0.0004 | 0.0011 | 0.0009 | 0.0008 | 0.0016 | 0.0025 | 0.0001 | 0.0005 | 0.0009 | 0.0017 | 0.0009 | 0.0022 | 0.0009 | 0.0008 | 0.0005 | 0.0005 | 0.0009 |
Vacuole Periphery | 0.0052 | 0.0007 | 0.0046 | 0.0025 | 0.003 | 0.0166 | 0.0135 | 0.0001 | 0.0014 | 0.002 | 0.0118 | 0.0139 | 0.0094 | 0.003 | 0.0026 | 0.0013 | 0.0012 | 0.0049 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 153.0407 | 112.6 | 103.6071 | 99.3575 | 97.9871 | 55.3915 | 83.1103 | 72.6147 | 53.5473 | 93.0162 |
Translational Efficiency | 1.3336 | 1.7768 | 1.5963 | 1.3044 | 1.6598 | 2.6393 | 1.7227 | 1.2333 | 1.6618 | 1.5835 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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