Standard name
Description Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.13 0 0 0 0.07 0 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.16 0.11 0.13 0.11 0.09 0.05 0.2 0.08 0.16 0.12 0.24 0.08 0.09 0 0 0 0 0.06 0.05
Nucleus 0.23 0.18 0.09 0 0.06 0.06 0.06 0.06 0.05 0.07 0.07 0 0 0 0.14 0.15 0.06 0.07 0 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.76 0.8 0.86 0.93 0.84 0.86 0.85 0.86 0.86 0.85 0.83 0.91 0.85 0.8 0.75 0.68 0.75 0.7 0.66 0.58
Peroxisomes 0 0 0 0 0 0.08 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.09 0.12 0.18 0.24 0.16 0.22 0.16 0.23 0.07 0.12 0.08 0 0 0.07 0 0.06 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1
Bud 0 2 0 0 0 0 2 5 2 4 5 0 0 0 3 3 6 2 3 8
Bud Neck 0 1 6 3 2 1 5 2 7 2 4 0 8 1 0 2 2 1 2 0
Bud Site 1 0 0 0 0 3 4 17 2 12 41 0 2 3
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 1 0 3 0 0 0 0 0 1
Cytoplasm 11 2 9 8 9 2 7 7 6 4 10 15 36 49 0 7 0 16 7 21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Endosome 0 3 3 2 12 23 56 9 27 34 38 2 3 5 0 7 9 2 7 9
Golgi 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 1 1 1 1 1
Mitochondria 93 70 63 52 49 56 47 123 53 152 108 52 28 36 5 14 20 5 14 13
Nucleus 97 80 52 8 28 33 51 38 36 62 64 4 16 12 60 72 34 17 9 16
Nuclear Periphery 1 2 2 0 3 5 4 6 4 5 4 0 1 0 15 7 11 2 4 7
Nucleolus 315 362 481 383 363 513 742 537 580 791 752 197 305 304 332 335 450 169 168 158
Peroxisomes 0 1 7 16 9 46 43 16 31 20 21 7 7 6 0 1 0 0 2 0
SpindlePole 1 1 24 35 53 110 211 98 146 151 213 16 42 29 3 12 40 9 16 9
Vac/Vac Membrane 22 12 14 3 9 6 15 0 7 9 9 1 1 2 19 22 11 7 11 15
Unique Cell Count 417 451 559 411 433 599 871 627 673 934 909 216 358 379 445 494 601 241 254 272
Labelled Cell Count 542 536 661 510 538 800 1187 858 901 1246 1269 295 449 450 445 494 601 241 254 272


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 35.0 23.5 24.1 23.1 14.9 15.7 14.5 12.7 13.7 12.7 12.4 25.8 20.2 21.9 29.0 28.3 27.5
Std Deviation (1e-4) 14.3 9.1 8.4 8.7 5.2 5.5 4.7 4.8 4.5 4.1 4.7 11.3 10.3 10.7 12.2 10.8 11.0
Intensity Change (Log2) -0.06 -0.69 -0.62 -0.73 -0.93 -0.81 -0.92 -0.96 0.1 -0.26 -0.14 0.27 0.23 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 -0.1 0 0 0 1.4 0
Bud Site 0 0 0 0 3.9 0 0 5.1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.5 -2.2 -1.4 -0.7 -1.1 0 -0.8 3.8 5.8 7.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.9 3.8 5.5 1.5 3.9 3.7 4.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 0 -1.1 -4.1 3.9 -2.0 2.7 0.4 4.5 -1.7 -0.9
Nucleus -4.7 -1.6 -2.5 -2.5 -2.1 -2.7 -1.9 -1.6 -3.6 -2.7 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.5 -1.0 -0.2 -0.4 -0.2 0.1 -0.7 -1.7 1.9 -0.4 -2.4
Peroxisomes 2.7 1.0 5.2 3.7 1.6 3.4 1.2 1.4 0 0.8 0.4
SpindlePole 2.7 4.6 7.5 9.9 6.4 8.8 6.9 9.7 1.8 4.3 2.2
Vacuole -2.1 -0.4 -2.0 -1.0 -4.0 -2.0 -2.3 -2.3 0 -2.6 -2.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Brx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available